Odonata-associated circular virus-3 
Average proteome isoelectric point is 7.35 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A0B4UI27|A0A0B4UI27_9VIRU ATP-dependent helicase Rep OS=Odonata-associated circular virus-3 OX=1592123 PE=3 SV=1MM1 pKa = 7.7  AEE3 pKa = 5.09  LIRR6 pKa = 11.84  TEE8 pKa = 4.1  NPKK11 pKa = 10.36  KK12 pKa = 10.06  RR13 pKa = 11.84  DD14 pKa = 3.72  RR15 pKa = 11.84  NWCFTINNWTPEE27 pKa = 4.1  EE28 pKa = 3.84  QDD30 pKa = 3.16  QLRR33 pKa = 11.84  TLVPADD39 pKa = 3.27  AEE41 pKa = 4.55  YY42 pKa = 10.41  IVWGRR47 pKa = 11.84  EE48 pKa = 3.65  EE49 pKa = 4.73  GDD51 pKa = 3.85  AEE53 pKa = 4.42  HH54 pKa = 6.72  TPHH57 pKa = 6.64  LQGFVKK63 pKa = 10.01  TKK65 pKa = 10.94  NEE67 pKa = 3.57  VSMKK71 pKa = 10.14  KK72 pKa = 10.14  LKK74 pKa = 10.68  KK75 pKa = 10.31  LGFARR80 pKa = 11.84  AHH82 pKa = 5.94  LEE84 pKa = 4.0  IMGGTHH90 pKa = 6.99  AAAIAYY96 pKa = 7.5  CKK98 pKa = 10.21  KK99 pKa = 10.67  DD100 pKa = 3.6  GDD102 pKa = 3.65  WEE104 pKa = 4.5  EE105 pKa = 4.73  YY106 pKa = 10.75  GNQPLQGYY114 pKa = 7.62  RR115 pKa = 11.84  TDD117 pKa = 4.16  LVAFKK122 pKa = 10.61  HH123 pKa = 5.52  QIEE126 pKa = 4.34  LGKK129 pKa = 10.55  RR130 pKa = 11.84  PLEE133 pKa = 4.04  VAEE136 pKa = 4.07  QDD138 pKa = 3.79  SHH140 pKa = 7.51  FGAYY144 pKa = 8.69  VKK146 pKa = 8.61  YY147 pKa = 10.48  HH148 pKa = 6.7  RR149 pKa = 11.84  GFDD152 pKa = 3.35  NYY154 pKa = 9.78  HH155 pKa = 5.67  AHH157 pKa = 5.56  IQEE160 pKa = 4.37  SKK162 pKa = 10.62  RR163 pKa = 11.84  LRR165 pKa = 11.84  TGFKK169 pKa = 9.92  PPQVYY174 pKa = 10.27  LRR176 pKa = 11.84  VGDD179 pKa = 3.97  PGVGKK184 pKa = 10.27  SRR186 pKa = 11.84  HH187 pKa = 5.6  VYY189 pKa = 8.07  DD190 pKa = 3.26  THH192 pKa = 8.4  GYY194 pKa = 7.39  EE195 pKa = 5.77  HH196 pKa = 7.16  VYY198 pKa = 8.82  TWEE201 pKa = 3.81  SDD203 pKa = 2.98  MGNFFDD209 pKa = 5.3  GYY211 pKa = 9.06  RR212 pKa = 11.84  GQSVVHH218 pKa = 6.54  FEE220 pKa = 4.26  DD221 pKa = 3.64  VQKK224 pKa = 11.34  GQLPPLAKK232 pKa = 10.4  FKK234 pKa = 10.81  RR235 pKa = 11.84  LLDD238 pKa = 3.5  GHH240 pKa = 6.18  PVRR243 pKa = 11.84  VSVKK247 pKa = 9.63  GSSQIWSPDD256 pKa = 3.34  YY257 pKa = 10.72  IYY259 pKa = 10.45  ISSNHH264 pKa = 6.3  KK265 pKa = 8.9  PCTWYY270 pKa = 10.09  DD271 pKa = 3.81  YY272 pKa = 11.63  KK273 pKa = 11.42  DD274 pKa = 3.44  GDD276 pKa = 3.94  YY277 pKa = 10.49  EE278 pKa = 6.36  AIMSRR283 pKa = 11.84  IKK285 pKa = 10.08  EE286 pKa = 3.87  ARR288 pKa = 11.84  VVYY291 pKa = 10.15  KK292 pKa = 10.55  DD293 pKa = 3.55  RR294 pKa = 11.84  PDD296 pKa = 3.62  EE297 pKa = 4.15  VFHH300 pKa = 6.11  TSSRR304 pKa = 11.84  FDD306 pKa = 2.88  AWFQAQEE313 pKa = 4.11  DD314 pKa = 3.91  QQ315 pKa = 4.4  
 36.72 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  6.403 
IPC2_protein 6.313 
IPC_protein 6.478 
Toseland    6.883 
ProMoST     6.81 
Dawson      6.825 
Bjellqvist  6.766 
Wikipedia   6.854 
Rodwell     6.825 
Grimsley    7.059 
Solomon     6.839 
Lehninger   6.839 
Nozaki      7.073 
DTASelect   7.263 
Thurlkill   7.293 
EMBOSS      7.293 
Sillero     7.22 
Patrickios  4.215 
IPC_peptide 6.854 
IPC2_peptide  6.971 
IPC2.peptide.svr19  6.93 
 Protein with the highest isoelectric point: 
>tr|A0A0B4UI27|A0A0B4UI27_9VIRU ATP-dependent helicase Rep OS=Odonata-associated circular virus-3 OX=1592123 PE=3 SV=1MM1 pKa = 7.9  LFGQKK6 pKa = 8.2  EE7 pKa = 3.89  DD8 pKa = 3.76  RR9 pKa = 11.84  MNVTYY14 pKa = 10.14  RR15 pKa = 11.84  VVVLKK20 pKa = 9.67  CTEE23 pKa = 4.04  DD24 pKa = 3.48  QQPSSTVDD32 pKa = 3.44  DD33 pKa = 5.04  LIEE36 pKa = 4.27  NVTGNILLDD45 pKa = 3.35  GTNRR49 pKa = 11.84  DD50 pKa = 3.41  RR51 pKa = 11.84  GNIVYY56 pKa = 10.06  QKK58 pKa = 10.06  YY59 pKa = 9.42  IKK61 pKa = 9.5  KK62 pKa = 8.92  TITPQLAPAGQEE74 pKa = 3.74  RR75 pKa = 11.84  EE76 pKa = 4.05  LTFTHH81 pKa = 6.8  KK82 pKa = 10.47  FWIPYY87 pKa = 9.21  KK88 pKa = 10.61  KK89 pKa = 10.43  LIKK92 pKa = 10.49  FSGNNGQQFSGPKK105 pKa = 9.44  LYY107 pKa = 10.73  LYY109 pKa = 10.6  VFAYY113 pKa = 10.12  DD114 pKa = 3.75  AYY116 pKa = 9.83  GTAITDD122 pKa = 3.93  NIAYY126 pKa = 9.03  YY127 pKa = 9.78  QMWHH131 pKa = 6.15  KK132 pKa = 10.66  LYY134 pKa = 11.01  YY135 pKa = 9.79  RR136 pKa = 11.84  DD137 pKa = 3.68  PP138 pKa = 4.83  
 16.14 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  8.306 
IPC2_protein 8.331 
IPC_protein 8.375 
Toseland    8.712 
ProMoST     8.697 
Dawson      9.019 
Bjellqvist  8.873 
Wikipedia   9.224 
Rodwell     9.092 
Grimsley    9.063 
Solomon     9.194 
Lehninger   9.136 
Nozaki      8.799 
DTASelect   8.829 
Thurlkill   8.858 
EMBOSS      9.136 
Sillero     9.033 
Patrickios  3.897 
IPC_peptide 9.18 
IPC2_peptide  7.629 
IPC2.peptide.svr19  7.666 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        2 
0
2 
453
138
315
226.5
26.43
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        5.519 ± 0.614
0.883 ± 0.083
7.064 ± 0.284
6.181 ± 1.342
4.415 ± 0.035
7.285 ± 0.4
3.974 ± 1.324
4.636 ± 0.989
7.726 ± 0.128
6.181 ± 0.939
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        1.766 ± 0.214
3.753 ± 1.072
4.636 ± 0.151
5.519 ± 0.526
5.74 ± 0.73
4.194 ± 0.68
5.298 ± 1.402
6.402 ± 0.317
2.208 ± 0.398
6.623 ± 1.087
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here