Amino acid dipepetide frequency for Methylobacterium sp. WL8

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.063AlaAla: 22.063 ± 0.19
1.363AlaCys: 1.363 ± 0.035
8.001AlaAsp: 8.001 ± 0.088
8.631AlaGlu: 8.631 ± 0.099
4.965AlaPhe: 4.965 ± 0.061
13.056AlaGly: 13.056 ± 0.167
2.45AlaHis: 2.45 ± 0.049
6.517AlaIle: 6.517 ± 0.072
3.897AlaLys: 3.897 ± 0.071
15.719AlaLeu: 15.719 ± 0.127
3.629AlaMet: 3.629 ± 0.047
2.718AlaAsn: 2.718 ± 0.063
7.25AlaPro: 7.25 ± 0.098
4.03AlaGln: 4.03 ± 0.057
11.058AlaArg: 11.058 ± 0.108
6.913AlaSer: 6.913 ± 0.07
7.225AlaThr: 7.225 ± 0.097
10.459AlaVal: 10.459 ± 0.098
1.773AlaTrp: 1.773 ± 0.035
2.836AlaTyr: 2.836 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
1.075CysAla: 1.075 ± 0.032
0.097CysCys: 0.097 ± 0.008
0.495CysAsp: 0.495 ± 0.017
0.429CysGlu: 0.429 ± 0.017
0.273CysPhe: 0.273 ± 0.012
0.875CysGly: 0.875 ± 0.029
0.184CysHis: 0.184 ± 0.013
0.314CysIle: 0.314 ± 0.016
0.154CysLys: 0.154 ± 0.011
0.901CysLeu: 0.901 ± 0.023
0.13CysMet: 0.13 ± 0.01
0.196CysAsn: 0.196 ± 0.013
0.461CysPro: 0.461 ± 0.018
0.179CysGln: 0.179 ± 0.01
0.731CysArg: 0.731 ± 0.024
0.378CysSer: 0.378 ± 0.015
0.424CysThr: 0.424 ± 0.014
0.587CysVal: 0.587 ± 0.021
0.092CysTrp: 0.092 ± 0.008
0.142CysTyr: 0.142 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
8.819AspAla: 8.819 ± 0.076
0.389AspCys: 0.389 ± 0.017
3.163AspAsp: 3.163 ± 0.059
2.951AspGlu: 2.951 ± 0.052
1.898AspPhe: 1.898 ± 0.038
5.999AspGly: 5.999 ± 0.066
1.24AspHis: 1.24 ± 0.031
2.19AspIle: 2.19 ± 0.045
1.357AspLys: 1.357 ± 0.039
6.659AspLeu: 6.659 ± 0.068
0.977AspMet: 0.977 ± 0.026
0.908AspAsn: 0.908 ± 0.025
4.038AspPro: 4.038 ± 0.06
1.359AspGln: 1.359 ± 0.032
4.852AspArg: 4.852 ± 0.058
1.772AspSer: 1.772 ± 0.033
2.539AspThr: 2.539 ± 0.044
4.198AspVal: 4.198 ± 0.054
0.832AspTrp: 0.832 ± 0.023
1.26AspTyr: 1.26 ± 0.031
0.0AspXaa: 0.0 ± 0.0
Glu
9.209GluAla: 9.209 ± 0.089
0.286GluCys: 0.286 ± 0.015
2.43GluAsp: 2.43 ± 0.046
2.358GluGlu: 2.358 ± 0.044
1.333GluPhe: 1.333 ± 0.033
4.333GluGly: 4.333 ± 0.06
1.072GluHis: 1.072 ± 0.033
2.931GluIle: 2.931 ± 0.048
1.541GluLys: 1.541 ± 0.04
4.026GluLeu: 4.026 ± 0.06
1.235GluMet: 1.235 ± 0.033
1.158GluAsn: 1.158 ± 0.029
3.081GluPro: 3.081 ± 0.051
1.505GluGln: 1.505 ± 0.036
5.398GluArg: 5.398 ± 0.069
2.093GluSer: 2.093 ± 0.038
3.65GluThr: 3.65 ± 0.058
3.633GluVal: 3.633 ± 0.049
0.526GluTrp: 0.526 ± 0.021
0.739GluTyr: 0.739 ± 0.026
0.0GluXaa: 0.0 ± 0.0
Phe
4.737PheAla: 4.737 ± 0.067
0.338PheCys: 0.338 ± 0.017
2.364PheAsp: 2.364 ± 0.042
1.93PheGlu: 1.93 ± 0.034
1.156PhePhe: 1.156 ± 0.036
3.615PheGly: 3.615 ± 0.062
0.684PheHis: 0.684 ± 0.025
1.128PheIle: 1.128 ± 0.034
0.82PheLys: 0.82 ± 0.024
3.195PheLeu: 3.195 ± 0.053
0.582PheMet: 0.582 ± 0.024
0.757PheAsn: 0.757 ± 0.022
1.534PhePro: 1.534 ± 0.034
0.82PheGln: 0.82 ± 0.025
2.307PheArg: 2.307 ± 0.039
2.028PheSer: 2.028 ± 0.038
1.908PheThr: 1.908 ± 0.038
2.897PheVal: 2.897 ± 0.049
0.451PheTrp: 0.451 ± 0.021
0.764PheTyr: 0.764 ± 0.027
0.0PheXaa: 0.0 ± 0.0
Gly
11.317GlyAla: 11.317 ± 0.134
0.83GlyCys: 0.83 ± 0.027
4.639GlyAsp: 4.639 ± 0.06
4.464GlyGlu: 4.464 ± 0.055
3.755GlyPhe: 3.755 ± 0.057
8.182GlyGly: 8.182 ± 0.152
1.941GlyHis: 1.941 ± 0.039
4.326GlyIle: 4.326 ± 0.062
2.634GlyLys: 2.634 ± 0.05
10.49GlyLeu: 10.49 ± 0.093
2.042GlyMet: 2.042 ± 0.041
1.878GlyAsn: 1.878 ± 0.064
4.292GlyPro: 4.292 ± 0.056
2.763GlyGln: 2.763 ± 0.053
7.789GlyArg: 7.789 ± 0.082
4.803GlySer: 4.803 ± 0.081
5.573GlyThr: 5.573 ± 0.191
6.29GlyVal: 6.29 ± 0.073
1.377GlyTrp: 1.377 ± 0.033
2.33GlyTyr: 2.33 ± 0.053
0.0GlyXaa: 0.0 ± 0.0
His
2.797HisAla: 2.797 ± 0.053
0.187HisCys: 0.187 ± 0.011
1.251HisAsp: 1.251 ± 0.03
0.916HisGlu: 0.916 ± 0.024
0.692HisPhe: 0.692 ± 0.021
2.04HisGly: 2.04 ± 0.045
0.565HisHis: 0.565 ± 0.026
0.615HisIle: 0.615 ± 0.023
0.33HisLys: 0.33 ± 0.015
2.073HisLeu: 2.073 ± 0.042
0.356HisMet: 0.356 ± 0.015
0.356HisAsn: 0.356 ± 0.016
1.445HisPro: 1.445 ± 0.034
0.471HisGln: 0.471 ± 0.019
1.639HisArg: 1.639 ± 0.041
0.692HisSer: 0.692 ± 0.024
0.754HisThr: 0.754 ± 0.022
1.549HisVal: 1.549 ± 0.032
0.293HisTrp: 0.293 ± 0.015
0.458HisTyr: 0.458 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
6.907IleAla: 6.907 ± 0.069
0.397IleCys: 0.397 ± 0.018
3.102IleAsp: 3.102 ± 0.046
3.14IleGlu: 3.14 ± 0.044
1.154IlePhe: 1.154 ± 0.028
4.305IleGly: 4.305 ± 0.057
0.817IleHis: 0.817 ± 0.024
1.306IleIle: 1.306 ± 0.035
1.032IleLys: 1.032 ± 0.033
4.271IleLeu: 4.271 ± 0.057
0.647IleMet: 0.647 ± 0.022
1.011IleAsn: 1.011 ± 0.031
2.099IlePro: 2.099 ± 0.042
1.101IleGln: 1.101 ± 0.03
3.222IleArg: 3.222 ± 0.052
1.928IleSer: 1.928 ± 0.039
2.295IleThr: 2.295 ± 0.052
3.842IleVal: 3.842 ± 0.06
0.441IleTrp: 0.441 ± 0.02
0.796IleTyr: 0.796 ± 0.025
0.0IleXaa: 0.0 ± 0.0
Lys
4.095LysAla: 4.095 ± 0.074
0.119LysCys: 0.119 ± 0.01
1.452LysAsp: 1.452 ± 0.041
1.077LysGlu: 1.077 ± 0.033
0.687LysPhe: 0.687 ± 0.021
2.307LysGly: 2.307 ± 0.044
0.424LysHis: 0.424 ± 0.018
1.182LysIle: 1.182 ± 0.031
0.837LysLys: 0.837 ± 0.031
2.545LysLeu: 2.545 ± 0.044
0.522LysMet: 0.522 ± 0.019
0.641LysAsn: 0.641 ± 0.02
2.059LysPro: 2.059 ± 0.046
0.688LysGln: 0.688 ± 0.022
2.155LysArg: 2.155 ± 0.044
1.361LysSer: 1.361 ± 0.033
1.515LysThr: 1.515 ± 0.038
2.077LysVal: 2.077 ± 0.043
0.233LysTrp: 0.233 ± 0.013
0.436LysTyr: 0.436 ± 0.02
0.0LysXaa: 0.0 ± 0.0
Leu
16.082LeuAla: 16.082 ± 0.15
0.941LeuCys: 0.941 ± 0.028
6.767LeuAsp: 6.767 ± 0.079
4.388LeuGlu: 4.388 ± 0.064
3.373LeuPhe: 3.373 ± 0.057
9.064LeuGly: 9.064 ± 0.085
1.914LeuHis: 1.914 ± 0.041
4.322LeuIle: 4.322 ± 0.053
2.995LeuLys: 2.995 ± 0.057
8.883LeuLeu: 8.883 ± 0.119
2.08LeuMet: 2.08 ± 0.039
2.179LeuAsn: 2.179 ± 0.037
5.953LeuPro: 5.953 ± 0.069
2.4LeuGln: 2.4 ± 0.041
7.739LeuArg: 7.739 ± 0.08
6.021LeuSer: 6.021 ± 0.061
5.651LeuThr: 5.651 ± 0.088
8.547LeuVal: 8.547 ± 0.088
1.087LeuTrp: 1.087 ± 0.027
1.96LeuTyr: 1.96 ± 0.042
0.0LeuXaa: 0.0 ± 0.0
Met
2.726MetAla: 2.726 ± 0.043
0.123MetCys: 0.123 ± 0.01
0.894MetAsp: 0.894 ± 0.023
0.911MetGlu: 0.911 ± 0.026
0.516MetPhe: 0.516 ± 0.021
1.468MetGly: 1.468 ± 0.037
0.384MetHis: 0.384 ± 0.016
1.022MetIle: 1.022 ± 0.031
0.679MetLys: 0.679 ± 0.02
2.148MetLeu: 2.148 ± 0.042
0.536MetMet: 0.536 ± 0.021
0.572MetAsn: 0.572 ± 0.019
1.58MetPro: 1.58 ± 0.035
0.682MetGln: 0.682 ± 0.021
2.02MetArg: 2.02 ± 0.043
1.416MetSer: 1.416 ± 0.031
1.529MetThr: 1.529 ± 0.035
1.435MetVal: 1.435 ± 0.033
0.154MetTrp: 0.154 ± 0.01
0.22MetTyr: 0.22 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
3.015AsnAla: 3.015 ± 0.081
0.169AsnCys: 0.169 ± 0.011
1.096AsnAsp: 1.096 ± 0.033
0.916AsnGlu: 0.916 ± 0.026
0.723AsnPhe: 0.723 ± 0.027
2.111AsnGly: 2.111 ± 0.074
0.441AsnHis: 0.441 ± 0.018
0.855AsnIle: 0.855 ± 0.026
0.509AsnLys: 0.509 ± 0.018
2.309AsnLeu: 2.309 ± 0.041
0.378AsnMet: 0.378 ± 0.017
0.535AsnAsn: 0.535 ± 0.025
1.637AsnPro: 1.637 ± 0.04
0.605AsnGln: 0.605 ± 0.026
1.659AsnArg: 1.659 ± 0.034
0.854AsnSer: 0.854 ± 0.036
1.099AsnThr: 1.099 ± 0.045
1.717AsnVal: 1.717 ± 0.039
0.273AsnTrp: 0.273 ± 0.016
0.477AsnTyr: 0.477 ± 0.02
0.0AsnXaa: 0.0 ± 0.0
Pro
8.099ProAla: 8.099 ± 0.102
0.362ProCys: 0.362 ± 0.016
4.203ProAsp: 4.203 ± 0.061
3.898ProGlu: 3.898 ± 0.064
2.022ProPhe: 2.022 ± 0.04
5.457ProGly: 5.457 ± 0.065
1.059ProHis: 1.059 ± 0.027
2.199ProIle: 2.199 ± 0.035
1.641ProLys: 1.641 ± 0.034
4.827ProLeu: 4.827 ± 0.062
1.251ProMet: 1.251 ± 0.03
1.276ProAsn: 1.276 ± 0.032
3.12ProPro: 3.12 ± 0.06
1.443ProGln: 1.443 ± 0.031
3.657ProArg: 3.657 ± 0.061
2.93ProSer: 2.93 ± 0.048
2.808ProThr: 2.808 ± 0.049
4.715ProVal: 4.715 ± 0.061
0.729ProTrp: 0.729 ± 0.028
1.129ProTyr: 1.129 ± 0.028
0.0ProXaa: 0.0 ± 0.0
Gln
4.443GlnAla: 4.443 ± 0.06
0.17GlnCys: 0.17 ± 0.011
1.361GlnAsp: 1.361 ± 0.032
1.112GlnGlu: 1.112 ± 0.032
0.837GlnPhe: 0.837 ± 0.024
2.481GlnGly: 2.481 ± 0.045
0.473GlnHis: 0.473 ± 0.017
1.394GlnIle: 1.394 ± 0.03
0.732GlnLys: 0.732 ± 0.027
2.103GlnLeu: 2.103 ± 0.036
0.56GlnMet: 0.56 ± 0.021
0.662GlnAsn: 0.662 ± 0.023
1.565GlnPro: 1.565 ± 0.037
0.828GlnGln: 0.828 ± 0.031
2.128GlnArg: 2.128 ± 0.043
1.413GlnSer: 1.413 ± 0.032
1.573GlnThr: 1.573 ± 0.034
2.297GlnVal: 2.297 ± 0.043
0.287GlnTrp: 0.287 ± 0.014
0.467GlnTyr: 0.467 ± 0.019
0.0GlnXaa: 0.0 ± 0.0
Arg
10.154ArgAla: 10.154 ± 0.096
0.541ArgCys: 0.541 ± 0.022
4.719ArgAsp: 4.719 ± 0.055
4.173ArgGlu: 4.173 ± 0.058
3.037ArgPhe: 3.037 ± 0.043
5.765ArgGly: 5.765 ± 0.067
1.868ArgHis: 1.868 ± 0.041
4.267ArgIle: 4.267 ± 0.053
1.981ArgLys: 1.981 ± 0.042
9.031ArgLeu: 9.031 ± 0.091
1.894ArgMet: 1.894 ± 0.041
1.702ArgAsn: 1.702 ± 0.034
4.546ArgPro: 4.546 ± 0.063
2.288ArgGln: 2.288 ± 0.043
7.118ArgArg: 7.118 ± 0.087
3.862ArgSer: 3.862 ± 0.057
4.299ArgThr: 4.299 ± 0.057
5.646ArgVal: 5.646 ± 0.068
1.031ArgTrp: 1.031 ± 0.029
1.715ArgTyr: 1.715 ± 0.035
0.0ArgXaa: 0.0 ± 0.0
Ser
6.426SerAla: 6.426 ± 0.075
0.381SerCys: 0.381 ± 0.017
2.742SerAsp: 2.742 ± 0.043
2.648SerGlu: 2.648 ± 0.041
1.85SerPhe: 1.85 ± 0.035
5.363SerGly: 5.363 ± 0.096
0.937SerHis: 0.937 ± 0.026
2.077SerIle: 2.077 ± 0.04
1.247SerLys: 1.247 ± 0.034
5.17SerLeu: 5.17 ± 0.062
0.948SerMet: 0.948 ± 0.026
1.113SerAsn: 1.113 ± 0.036
2.787SerPro: 2.787 ± 0.041
1.34SerGln: 1.34 ± 0.031
3.589SerArg: 3.589 ± 0.054
2.356SerSer: 2.356 ± 0.049
2.543SerThr: 2.543 ± 0.055
3.812SerVal: 3.812 ± 0.055
0.593SerTrp: 0.593 ± 0.021
1.077SerTyr: 1.077 ± 0.029
0.0SerXaa: 0.0 ± 0.0
Thr
6.998ThrAla: 6.998 ± 0.086
0.443ThrCys: 0.443 ± 0.019
2.719ThrAsp: 2.719 ± 0.045
2.535ThrGlu: 2.535 ± 0.046
1.919ThrPhe: 1.919 ± 0.041
5.53ThrGly: 5.53 ± 0.135
0.954ThrHis: 0.954 ± 0.027
2.737ThrIle: 2.737 ± 0.055
1.405ThrLys: 1.405 ± 0.033
6.505ThrLeu: 6.505 ± 0.087
1.039ThrMet: 1.039 ± 0.026
1.23ThrAsn: 1.23 ± 0.068
3.373ThrPro: 3.373 ± 0.049
1.432ThrGln: 1.432 ± 0.036
3.713ThrArg: 3.713 ± 0.05
2.44ThrSer: 2.44 ± 0.046
2.982ThrThr: 2.982 ± 0.069
4.857ThrVal: 4.857 ± 0.088
0.686ThrTrp: 0.686 ± 0.019
1.229ThrTyr: 1.229 ± 0.033
0.0ThrXaa: 0.0 ± 0.0
Val
11.559ValAla: 11.559 ± 0.09
0.633ValCys: 0.633 ± 0.021
4.071ValAsp: 4.071 ± 0.054
4.553ValGlu: 4.553 ± 0.063
2.609ValPhe: 2.609 ± 0.044
6.731ValGly: 6.731 ± 0.066
1.376ValHis: 1.376 ± 0.032
3.273ValIle: 3.273 ± 0.051
1.875ValLys: 1.875 ± 0.044
7.834ValLeu: 7.834 ± 0.092
1.606ValMet: 1.606 ± 0.036
1.669ValAsn: 1.669 ± 0.049
4.391ValPro: 4.391 ± 0.062
2.008ValGln: 2.008 ± 0.037
5.754ValArg: 5.754 ± 0.066
4.123ValSer: 4.123 ± 0.048
4.616ValThr: 4.616 ± 0.069
6.926ValVal: 6.926 ± 0.079
0.864ValTrp: 0.864 ± 0.026
1.356ValTyr: 1.356 ± 0.032
0.0ValXaa: 0.0 ± 0.0
Trp
1.219TrpAla: 1.219 ± 0.035
0.14TrpCys: 0.14 ± 0.01
0.585TrpAsp: 0.585 ± 0.023
0.44TrpGlu: 0.44 ± 0.016
0.496TrpPhe: 0.496 ± 0.02
0.808TrpGly: 0.808 ± 0.02
0.298TrpHis: 0.298 ± 0.013
0.591TrpIle: 0.591 ± 0.02
0.3TrpLys: 0.3 ± 0.012
1.519TrpLeu: 1.519 ± 0.035
0.305TrpMet: 0.305 ± 0.014
0.362TrpAsn: 0.362 ± 0.017
0.652TrpPro: 0.652 ± 0.023
0.417TrpGln: 0.417 ± 0.019
1.268TrpArg: 1.268 ± 0.031
0.775TrpSer: 0.775 ± 0.023
0.781TrpThr: 0.781 ± 0.023
0.76TrpVal: 0.76 ± 0.023
0.214TrpTrp: 0.214 ± 0.011
0.273TrpTyr: 0.273 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.813TyrAla: 2.813 ± 0.046
0.21TyrCys: 0.21 ± 0.012
1.347TyrAsp: 1.347 ± 0.032
1.065TyrGlu: 1.065 ± 0.028
0.72TyrPhe: 0.72 ± 0.022
2.222TyrGly: 2.222 ± 0.05
0.399TyrHis: 0.399 ± 0.016
0.566TyrIle: 0.566 ± 0.021
0.45TyrLys: 0.45 ± 0.017
2.136TyrLeu: 2.136 ± 0.037
0.299TyrMet: 0.299 ± 0.013
0.455TyrAsn: 0.455 ± 0.019
0.964TyrPro: 0.964 ± 0.027
0.525TyrGln: 0.525 ± 0.02
1.841TyrArg: 1.841 ± 0.036
0.838TyrSer: 0.838 ± 0.022
0.964TyrThr: 0.964 ± 0.031
1.584TyrVal: 1.584 ± 0.033
0.269TyrTrp: 0.269 ± 0.014
0.452TyrTyr: 0.452 ± 0.018
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4923 proteins (1474374 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski