Plantago lanceolata latent virus
Average proteome isoelectric point is 7.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A166V5R3|A0A166V5R3_9GEMI Replication-associated protein OS=Plantago lanceolata latent virus OX=1830242 PE=3 SV=1
MM1 pKa = 7.62 PRR3 pKa = 11.84 QPLTYY8 pKa = 10.0 RR9 pKa = 11.84 FQGKK13 pKa = 8.62 SAFLTYY19 pKa = 9.2 PKK21 pKa = 10.34 CDD23 pKa = 3.57 LTPTSVLDD31 pKa = 3.44 YY32 pKa = 10.88 LYY34 pKa = 11.3 NLLKK38 pKa = 10.91 NYY40 pKa = 10.16 DD41 pKa = 2.97 IFYY44 pKa = 10.99 ARR46 pKa = 11.84 VCQEE50 pKa = 3.42 NHH52 pKa = 5.66 QDD54 pKa = 3.62 GTKK57 pKa = 10.14 HH58 pKa = 5.53 LHH60 pKa = 6.69 CLVQLAKK67 pKa = 10.69 RR68 pKa = 11.84 LTTRR72 pKa = 11.84 DD73 pKa = 3.39 PKK75 pKa = 11.36 YY76 pKa = 10.98 FDD78 pKa = 2.88 ITTANTITSGDD89 pKa = 3.95 AQPHH93 pKa = 5.96 HH94 pKa = 7.34 PNIQVPRR101 pKa = 11.84 SDD103 pKa = 3.46 AHH105 pKa = 5.36 VADD108 pKa = 5.39 YY109 pKa = 10.38 IAKK112 pKa = 10.19 DD113 pKa = 3.47 GNFVEE118 pKa = 4.93 RR119 pKa = 11.84 GVLKK123 pKa = 9.79 ATRR126 pKa = 11.84 RR127 pKa = 11.84 SPKK130 pKa = 9.83 KK131 pKa = 10.49 SRR133 pKa = 11.84 DD134 pKa = 4.09 TIWTAILQEE143 pKa = 4.52 STSKK147 pKa = 10.73 PDD149 pKa = 3.22 FLARR153 pKa = 11.84 VQSEE157 pKa = 4.23 QPYY160 pKa = 8.52 TWATQLRR167 pKa = 11.84 NLEE170 pKa = 4.05 YY171 pKa = 10.28 AANSRR176 pKa = 11.84 WPEE179 pKa = 3.72 PPVLYY184 pKa = 9.99 EE185 pKa = 3.41 PRR187 pKa = 11.84 YY188 pKa = 9.56 RR189 pKa = 11.84 AFPNLPEE196 pKa = 5.47 PIKK199 pKa = 10.65 QWAEE203 pKa = 3.85 TNLYY207 pKa = 8.41 TVSFNSMQLIDD218 pKa = 4.76 PDD220 pKa = 4.16 IEE222 pKa = 4.46 HH223 pKa = 7.61 ADD225 pKa = 4.19 LDD227 pKa = 4.22 WASNTAQEE235 pKa = 4.25 EE236 pKa = 4.47 LEE238 pKa = 4.18 EE239 pKa = 3.93 QLLLNSQQPITVSDD253 pKa = 4.43 AGPQTT258 pKa = 3.5
Molecular weight: 29.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.692
IPC2_protein 5.69
IPC_protein 5.728
Toseland 6.148
ProMoST 6.059
Dawson 5.982
Bjellqvist 5.982
Wikipedia 5.982
Rodwell 5.957
Grimsley 6.313
Solomon 5.982
Lehninger 5.97
Nozaki 6.237
DTASelect 6.427
Thurlkill 6.427
EMBOSS 6.402
Sillero 6.338
Patrickios 3.973
IPC_peptide 5.995
IPC2_peptide 6.326
IPC2.peptide.svr19 6.288
Protein with the highest isoelectric point:
>tr|A0A166V5P1|A0A166V5P1_9GEMI C3 OS=Plantago lanceolata latent virus OX=1830242 PE=4 SV=1
MM1 pKa = 7.38 VKK3 pKa = 10.13 RR4 pKa = 11.84 KK5 pKa = 9.88 RR6 pKa = 11.84 STGVTTTYY14 pKa = 11.07 AKK16 pKa = 10.26 RR17 pKa = 11.84 SRR19 pKa = 11.84 VGSGYY24 pKa = 9.23 RR25 pKa = 11.84 RR26 pKa = 11.84 RR27 pKa = 11.84 WSRR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 RR33 pKa = 11.84 IVRR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.55 GVYY41 pKa = 9.24 ARR43 pKa = 11.84 HH44 pKa = 6.21 RR45 pKa = 11.84 SQSVLHH51 pKa = 6.16 SLSNATATKK60 pKa = 10.46 GKK62 pKa = 10.28 AGYY65 pKa = 8.93 GWHH68 pKa = 6.78 LNEE71 pKa = 4.7 IPLGSSYY78 pKa = 10.73 PEE80 pKa = 3.67 RR81 pKa = 11.84 HH82 pKa = 5.41 SDD84 pKa = 3.24 KK85 pKa = 11.26 VKK87 pKa = 10.02 VKK89 pKa = 9.98 SFRR92 pKa = 11.84 FRR94 pKa = 11.84 MQFRR98 pKa = 11.84 DD99 pKa = 3.58 GANGGTNAANVHH111 pKa = 5.68 NLYY114 pKa = 10.72 LYY116 pKa = 10.34 LVKK119 pKa = 10.73 DD120 pKa = 3.66 NSGGTEE126 pKa = 3.81 VPSFDD131 pKa = 4.81 KK132 pKa = 10.72 IAMMDD137 pKa = 3.65 YY138 pKa = 11.02 GNVATAIIDD147 pKa = 3.51 HH148 pKa = 7.0 DD149 pKa = 4.1 SEE151 pKa = 4.28 KK152 pKa = 10.68 RR153 pKa = 11.84 FTIVRR158 pKa = 11.84 QWKK161 pKa = 7.72 YY162 pKa = 9.11 TFTGSCNSQYY172 pKa = 10.15 PGKK175 pKa = 10.46 DD176 pKa = 2.51 RR177 pKa = 11.84 IDD179 pKa = 4.13 FNDD182 pKa = 3.08 VVYY185 pKa = 10.91 INVDD189 pKa = 3.68 TEE191 pKa = 4.55 FKK193 pKa = 10.75 SATDD197 pKa = 3.32 GKK199 pKa = 10.72 YY200 pKa = 11.07 ANTQKK205 pKa = 10.77 NAWVLYY211 pKa = 9.65 IIPQSYY217 pKa = 9.9 DD218 pKa = 3.32 CVIDD222 pKa = 3.0 GHH224 pKa = 5.74 VNVRR228 pKa = 11.84 FEE230 pKa = 4.3 SLVV233 pKa = 3.3
Molecular weight: 26.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.261
IPC2_protein 9.633
IPC_protein 9.97
Toseland 10.16
ProMoST 9.897
Dawson 10.379
Bjellqvist 10.072
Wikipedia 10.57
Rodwell 10.716
Grimsley 10.467
Solomon 10.409
Lehninger 10.365
Nozaki 10.145
DTASelect 10.072
Thurlkill 10.218
EMBOSS 10.57
Sillero 10.292
Patrickios 10.233
IPC_peptide 10.409
IPC2_peptide 8.843
IPC2.peptide.svr19 8.64
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1196
86
335
199.3
22.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.355 ± 0.756
1.589 ± 0.414
5.1 ± 0.778
5.435 ± 1.016
3.261 ± 0.372
4.515 ± 0.955
2.425 ± 0.394
5.1 ± 0.347
5.268 ± 0.957
8.027 ± 1.104
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.421 ± 0.342
4.849 ± 0.409
6.522 ± 1.594
5.1 ± 0.82
7.358 ± 0.865
7.776 ± 1.773
7.441 ± 1.04
5.268 ± 1.007
1.756 ± 0.232
5.435 ± 0.434
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here