Amino acid dipepetide frequency for Mycoplasma putrefaciens

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.219AlaAla: 3.219 ± 0.157
0.609AlaCys: 0.609 ± 0.051
2.995AlaAsp: 2.995 ± 0.122
3.041AlaGlu: 3.041 ± 0.144
2.489AlaPhe: 2.489 ± 0.123
3.253AlaGly: 3.253 ± 0.147
0.793AlaHis: 0.793 ± 0.065
5.857AlaIle: 5.857 ± 0.202
6.248AlaLys: 6.248 ± 0.196
5.213AlaLeu: 5.213 ± 0.157
1.121AlaMet: 1.121 ± 0.078
3.771AlaAsn: 3.771 ± 0.133
1.224AlaPro: 1.224 ± 0.086
1.753AlaGln: 1.753 ± 0.103
2.035AlaArg: 2.035 ± 0.123
3.506AlaSer: 3.506 ± 0.138
3.075AlaThr: 3.075 ± 0.111
3.42AlaVal: 3.42 ± 0.151
0.322AlaTrp: 0.322 ± 0.046
2.017AlaTyr: 2.017 ± 0.1
0.0AlaXaa: 0.0 ± 0.0
Cys
0.305CysAla: 0.305 ± 0.042
0.069CysCys: 0.069 ± 0.019
0.402CysAsp: 0.402 ± 0.046
0.368CysGlu: 0.368 ± 0.05
0.362CysPhe: 0.362 ± 0.048
0.414CysGly: 0.414 ± 0.043
0.103CysHis: 0.103 ± 0.024
0.425CysIle: 0.425 ± 0.051
0.414CysLys: 0.414 ± 0.046
0.707CysLeu: 0.707 ± 0.062
0.109CysMet: 0.109 ± 0.022
0.414CysAsn: 0.414 ± 0.045
0.149CysPro: 0.149 ± 0.03
0.339CysGln: 0.339 ± 0.044
0.092CysArg: 0.092 ± 0.023
0.448CysSer: 0.448 ± 0.053
0.351CysThr: 0.351 ± 0.039
0.287CysVal: 0.287 ± 0.045
0.063CysTrp: 0.063 ± 0.018
0.218CysTyr: 0.218 ± 0.034
0.0CysXaa: 0.0 ± 0.0
Asp
2.891AspAla: 2.891 ± 0.132
0.259AspCys: 0.259 ± 0.04
3.127AspAsp: 3.127 ± 0.143
3.443AspGlu: 3.443 ± 0.162
3.541AspPhe: 3.541 ± 0.156
2.299AspGly: 2.299 ± 0.115
1.178AspHis: 1.178 ± 0.089
4.495AspIle: 4.495 ± 0.179
5.713AspLys: 5.713 ± 0.196
6.857AspLeu: 6.857 ± 0.212
0.908AspMet: 0.908 ± 0.07
3.529AspAsn: 3.529 ± 0.151
1.73AspPro: 1.73 ± 0.099
4.15AspGln: 4.15 ± 0.158
1.356AspArg: 1.356 ± 0.095
3.552AspSer: 3.552 ± 0.132
1.897AspThr: 1.897 ± 0.121
3.374AspVal: 3.374 ± 0.155
0.466AspTrp: 0.466 ± 0.053
2.644AspTyr: 2.644 ± 0.122
0.0AspXaa: 0.0 ± 0.0
Glu
2.88GluAla: 2.88 ± 0.149
0.276GluCys: 0.276 ± 0.041
2.219GluAsp: 2.219 ± 0.123
3.495GluGlu: 3.495 ± 0.155
3.276GluPhe: 3.276 ± 0.133
1.96GluGly: 1.96 ± 0.111
0.793GluHis: 0.793 ± 0.06
7.667GluIle: 7.667 ± 0.227
5.782GluLys: 5.782 ± 0.209
6.937GluLeu: 6.937 ± 0.209
1.42GluMet: 1.42 ± 0.088
4.035GluAsn: 4.035 ± 0.146
1.701GluPro: 1.701 ± 0.123
3.38GluGln: 3.38 ± 0.154
1.627GluArg: 1.627 ± 0.094
3.058GluSer: 3.058 ± 0.126
2.747GluThr: 2.747 ± 0.123
3.759GluVal: 3.759 ± 0.165
0.356GluTrp: 0.356 ± 0.05
2.115GluTyr: 2.115 ± 0.103
0.0GluXaa: 0.0 ± 0.0
Phe
2.811PheAla: 2.811 ± 0.129
0.431PheCys: 0.431 ± 0.053
3.725PheAsp: 3.725 ± 0.141
3.219PheGlu: 3.219 ± 0.132
2.138PhePhe: 2.138 ± 0.125
2.42PheGly: 2.42 ± 0.12
0.512PheHis: 0.512 ± 0.064
4.684PheIle: 4.684 ± 0.2
5.213PheLys: 5.213 ± 0.159
4.788PheLeu: 4.788 ± 0.172
0.77PheMet: 0.77 ± 0.07
3.736PheAsn: 3.736 ± 0.156
0.977PhePro: 0.977 ± 0.079
1.466PheGln: 1.466 ± 0.08
1.253PheArg: 1.253 ± 0.081
3.627PheSer: 3.627 ± 0.157
2.506PheThr: 2.506 ± 0.127
3.098PheVal: 3.098 ± 0.144
0.316PheTrp: 0.316 ± 0.047
1.765PheTyr: 1.765 ± 0.102
0.0PheXaa: 0.0 ± 0.0
Gly
2.995GlyAla: 2.995 ± 0.165
0.356GlyCys: 0.356 ± 0.044
2.23GlyAsp: 2.23 ± 0.113
2.46GlyGlu: 2.46 ± 0.146
2.546GlyPhe: 2.546 ± 0.127
2.759GlyGly: 2.759 ± 0.152
0.69GlyHis: 0.69 ± 0.059
4.661GlyIle: 4.661 ± 0.15
3.771GlyLys: 3.771 ± 0.164
4.834GlyLeu: 4.834 ± 0.168
1.219GlyMet: 1.219 ± 0.07
2.121GlyAsn: 2.121 ± 0.116
1.138GlyPro: 1.138 ± 0.084
1.776GlyGln: 1.776 ± 0.101
1.667GlyArg: 1.667 ± 0.103
3.311GlySer: 3.311 ± 0.136
2.563GlyThr: 2.563 ± 0.13
3.374GlyVal: 3.374 ± 0.14
0.362GlyTrp: 0.362 ± 0.045
2.104GlyTyr: 2.104 ± 0.113
0.0GlyXaa: 0.0 ± 0.0
His
0.862HisAla: 0.862 ± 0.073
0.132HisCys: 0.132 ± 0.028
0.701HisAsp: 0.701 ± 0.071
0.678HisGlu: 0.678 ± 0.061
0.701HisPhe: 0.701 ± 0.058
0.833HisGly: 0.833 ± 0.076
0.276HisHis: 0.276 ± 0.048
1.31HisIle: 1.31 ± 0.078
1.276HisLys: 1.276 ± 0.086
1.425HisLeu: 1.425 ± 0.079
0.207HisMet: 0.207 ± 0.033
1.04HisAsn: 1.04 ± 0.081
0.598HisPro: 0.598 ± 0.054
0.764HisGln: 0.764 ± 0.069
0.431HisArg: 0.431 ± 0.048
0.805HisSer: 0.805 ± 0.062
0.621HisThr: 0.621 ± 0.056
0.655HisVal: 0.655 ± 0.059
0.08HisTrp: 0.08 ± 0.021
0.437HisTyr: 0.437 ± 0.051
0.0HisXaa: 0.0 ± 0.0
Ile
5.949IleAla: 5.949 ± 0.2
0.793IleCys: 0.793 ± 0.067
6.271IleAsp: 6.271 ± 0.208
5.598IleGlu: 5.598 ± 0.173
4.633IlePhe: 4.633 ± 0.176
4.851IleGly: 4.851 ± 0.171
0.897IleHis: 0.897 ± 0.073
8.92IleIle: 8.92 ± 0.262
9.668IleLys: 9.668 ± 0.24
8.645IleLeu: 8.645 ± 0.216
1.362IleMet: 1.362 ± 0.078
7.277IleAsn: 7.277 ± 0.184
2.655IlePro: 2.655 ± 0.123
3.023IleGln: 3.023 ± 0.122
2.983IleArg: 2.983 ± 0.128
7.357IleSer: 7.357 ± 0.227
5.65IleThr: 5.65 ± 0.172
5.788IleVal: 5.788 ± 0.212
0.448IleTrp: 0.448 ± 0.055
3.368IleTyr: 3.368 ± 0.147
0.0IleXaa: 0.0 ± 0.0
Lys
5.133LysAla: 5.133 ± 0.172
0.299LysCys: 0.299 ± 0.043
6.156LysAsp: 6.156 ± 0.188
6.38LysGlu: 6.38 ± 0.22
4.133LysPhe: 4.133 ± 0.168
3.42LysGly: 3.42 ± 0.147
1.408LysHis: 1.408 ± 0.095
9.898LysIle: 9.898 ± 0.231
9.961LysLys: 9.961 ± 0.27
8.949LysLeu: 8.949 ± 0.245
2.017LysMet: 2.017 ± 0.11
8.104LysAsn: 8.104 ± 0.219
2.885LysPro: 2.885 ± 0.108
5.725LysGln: 5.725 ± 0.162
2.724LysArg: 2.724 ± 0.16
5.288LysSer: 5.288 ± 0.175
6.409LysThr: 6.409 ± 0.189
5.828LysVal: 5.828 ± 0.175
0.644LysTrp: 0.644 ± 0.063
4.213LysTyr: 4.213 ± 0.157
0.0LysXaa: 0.0 ± 0.0
Leu
5.868LeuAla: 5.868 ± 0.183
0.466LeuCys: 0.466 ± 0.051
6.88LeuAsp: 6.88 ± 0.206
7.248LeuGlu: 7.248 ± 0.206
4.627LeuPhe: 4.627 ± 0.166
4.541LeuGly: 4.541 ± 0.178
1.046LeuHis: 1.046 ± 0.079
9.553LeuIle: 9.553 ± 0.262
10.484LeuLys: 10.484 ± 0.269
9.104LeuLeu: 9.104 ± 0.263
1.69LeuMet: 1.69 ± 0.1
7.202LeuAsn: 7.202 ± 0.206
2.563LeuPro: 2.563 ± 0.122
2.88LeuGln: 2.88 ± 0.123
2.719LeuArg: 2.719 ± 0.112
6.759LeuSer: 6.759 ± 0.203
6.357LeuThr: 6.357 ± 0.182
6.11LeuVal: 6.11 ± 0.182
0.546LeuTrp: 0.546 ± 0.05
2.868LeuTyr: 2.868 ± 0.148
0.0LeuXaa: 0.0 ± 0.0
Met
1.379MetAla: 1.379 ± 0.09
0.121MetCys: 0.121 ± 0.024
0.948MetAsp: 0.948 ± 0.061
0.563MetGlu: 0.563 ± 0.058
1.397MetPhe: 1.397 ± 0.083
1.138MetGly: 1.138 ± 0.094
0.368MetHis: 0.368 ± 0.044
2.707MetIle: 2.707 ± 0.134
2.299MetLys: 2.299 ± 0.123
2.563MetLeu: 2.563 ± 0.127
0.425MetMet: 0.425 ± 0.051
1.575MetAsn: 1.575 ± 0.098
0.931MetPro: 0.931 ± 0.065
1.115MetGln: 1.115 ± 0.085
0.69MetArg: 0.69 ± 0.062
1.535MetSer: 1.535 ± 0.096
1.282MetThr: 1.282 ± 0.092
1.362MetVal: 1.362 ± 0.094
0.115MetTrp: 0.115 ± 0.029
0.759MetTyr: 0.759 ± 0.082
0.0MetXaa: 0.0 ± 0.0
Asn
3.178AsnAla: 3.178 ± 0.146
0.322AsnCys: 0.322 ± 0.045
4.0AsnAsp: 4.0 ± 0.152
3.437AsnGlu: 3.437 ± 0.137
3.748AsnPhe: 3.748 ± 0.152
2.903AsnGly: 2.903 ± 0.125
1.523AsnHis: 1.523 ± 0.09
5.897AsnIle: 5.897 ± 0.192
8.185AsnLys: 8.185 ± 0.233
7.127AsnLeu: 7.127 ± 0.183
0.931AsnMet: 0.931 ± 0.07
6.144AsnAsn: 6.144 ± 0.248
2.305AsnPro: 2.305 ± 0.105
4.477AsnGln: 4.477 ± 0.168
1.971AsnArg: 1.971 ± 0.103
4.569AsnSer: 4.569 ± 0.167
3.029AsnThr: 3.029 ± 0.116
3.155AsnVal: 3.155 ± 0.15
0.598AsnTrp: 0.598 ± 0.057
3.058AsnTyr: 3.058 ± 0.15
0.0AsnXaa: 0.0 ± 0.0
Pro
1.362ProAla: 1.362 ± 0.087
0.103ProCys: 0.103 ± 0.026
1.54ProAsp: 1.54 ± 0.114
2.029ProGlu: 2.029 ± 0.126
1.247ProPhe: 1.247 ± 0.084
1.489ProGly: 1.489 ± 0.095
0.408ProHis: 0.408 ± 0.045
2.868ProIle: 2.868 ± 0.13
2.397ProLys: 2.397 ± 0.114
2.334ProLeu: 2.334 ± 0.112
0.437ProMet: 0.437 ± 0.056
2.092ProAsn: 2.092 ± 0.104
0.477ProPro: 0.477 ± 0.063
0.868ProGln: 0.868 ± 0.087
0.776ProArg: 0.776 ± 0.076
1.971ProSer: 1.971 ± 0.113
1.971ProThr: 1.971 ± 0.114
1.73ProVal: 1.73 ± 0.099
0.109ProTrp: 0.109 ± 0.025
0.782ProTyr: 0.782 ± 0.076
0.0ProXaa: 0.0 ± 0.0
Gln
3.161GlnAla: 3.161 ± 0.145
0.103GlnCys: 0.103 ± 0.026
2.046GlnAsp: 2.046 ± 0.107
3.046GlnGlu: 3.046 ± 0.148
1.971GlnPhe: 1.971 ± 0.091
1.598GlnGly: 1.598 ± 0.093
0.546GlnHis: 0.546 ± 0.06
4.564GlnIle: 4.564 ± 0.177
4.529GlnLys: 4.529 ± 0.163
4.443GlnLeu: 4.443 ± 0.156
0.661GlnMet: 0.661 ± 0.055
3.259GlnAsn: 3.259 ± 0.128
1.121GlnPro: 1.121 ± 0.1
2.546GlnGln: 2.546 ± 0.145
1.408GlnArg: 1.408 ± 0.088
1.994GlnSer: 1.994 ± 0.109
2.926GlnThr: 2.926 ± 0.137
2.655GlnVal: 2.655 ± 0.117
0.276GlnTrp: 0.276 ± 0.037
1.437GlnTyr: 1.437 ± 0.097
0.0GlnXaa: 0.0 ± 0.0
Arg
1.558ArgAla: 1.558 ± 0.096
0.213ArgCys: 0.213 ± 0.035
1.546ArgAsp: 1.546 ± 0.098
1.937ArgGlu: 1.937 ± 0.106
1.644ArgPhe: 1.644 ± 0.096
1.379ArgGly: 1.379 ± 0.09
0.437ArgHis: 0.437 ± 0.045
2.615ArgIle: 2.615 ± 0.118
2.529ArgLys: 2.529 ± 0.111
2.816ArgLeu: 2.816 ± 0.114
0.856ArgMet: 0.856 ± 0.077
1.627ArgAsn: 1.627 ± 0.104
0.977ArgPro: 0.977 ± 0.079
1.42ArgGln: 1.42 ± 0.09
1.104ArgArg: 1.104 ± 0.082
1.719ArgSer: 1.719 ± 0.096
1.494ArgThr: 1.494 ± 0.105
1.799ArgVal: 1.799 ± 0.104
0.213ArgTrp: 0.213 ± 0.036
1.138ArgTyr: 1.138 ± 0.08
0.0ArgXaa: 0.0 ± 0.0
Ser
3.541SerAla: 3.541 ± 0.131
0.471SerCys: 0.471 ± 0.06
3.477SerAsp: 3.477 ± 0.124
4.029SerGlu: 4.029 ± 0.136
3.339SerPhe: 3.339 ± 0.172
3.535SerGly: 3.535 ± 0.153
0.77SerHis: 0.77 ± 0.061
5.702SerIle: 5.702 ± 0.186
6.391SerLys: 6.391 ± 0.191
7.018SerLeu: 7.018 ± 0.213
1.092SerMet: 1.092 ± 0.079
4.363SerAsn: 4.363 ± 0.2
1.466SerPro: 1.466 ± 0.109
2.552SerGln: 2.552 ± 0.114
1.793SerArg: 1.793 ± 0.108
4.771SerSer: 4.771 ± 0.166
3.489SerThr: 3.489 ± 0.141
3.684SerVal: 3.684 ± 0.145
0.604SerTrp: 0.604 ± 0.05
2.483SerTyr: 2.483 ± 0.117
0.0SerXaa: 0.0 ± 0.0
Thr
2.949ThrAla: 2.949 ± 0.138
0.241ThrCys: 0.241 ± 0.04
3.127ThrAsp: 3.127 ± 0.119
2.736ThrGlu: 2.736 ± 0.117
2.604ThrPhe: 2.604 ± 0.122
3.259ThrGly: 3.259 ± 0.149
0.713ThrHis: 0.713 ± 0.06
5.397ThrIle: 5.397 ± 0.159
5.437ThrLys: 5.437 ± 0.197
4.771ThrLeu: 4.771 ± 0.174
0.851ThrMet: 0.851 ± 0.072
4.431ThrAsn: 4.431 ± 0.159
1.874ThrPro: 1.874 ± 0.141
1.719ThrGln: 1.719 ± 0.099
1.615ThrArg: 1.615 ± 0.095
3.828ThrSer: 3.828 ± 0.126
3.449ThrThr: 3.449 ± 0.155
3.184ThrVal: 3.184 ± 0.146
0.299ThrTrp: 0.299 ± 0.044
1.719ThrTyr: 1.719 ± 0.103
0.0ThrXaa: 0.0 ± 0.0
Val
3.719ValAla: 3.719 ± 0.141
0.477ValCys: 0.477 ± 0.056
3.627ValAsp: 3.627 ± 0.154
3.977ValGlu: 3.977 ± 0.157
2.96ValPhe: 2.96 ± 0.143
3.316ValGly: 3.316 ± 0.143
0.713ValHis: 0.713 ± 0.061
5.805ValIle: 5.805 ± 0.172
5.506ValLys: 5.506 ± 0.21
6.259ValLeu: 6.259 ± 0.181
1.058ValMet: 1.058 ± 0.082
3.684ValAsn: 3.684 ± 0.155
1.448ValPro: 1.448 ± 0.085
1.793ValGln: 1.793 ± 0.111
1.684ValArg: 1.684 ± 0.108
4.087ValSer: 4.087 ± 0.131
2.615ValThr: 2.615 ± 0.118
4.477ValVal: 4.477 ± 0.178
0.299ValTrp: 0.299 ± 0.045
2.104ValTyr: 2.104 ± 0.108
0.0ValXaa: 0.0 ± 0.0
Trp
0.017TrpAla: 0.017 ± 0.009
0.011TrpCys: 0.011 ± 0.007
0.011TrpAsp: 0.011 ± 0.008
0.006TrpGlu: 0.006 ± 0.006
0.086TrpPhe: 0.086 ± 0.024
0.023TrpGly: 0.023 ± 0.011
0.011TrpHis: 0.011 ± 0.008
0.121TrpIle: 0.121 ± 0.028
0.034TrpLys: 0.034 ± 0.014
0.138TrpLeu: 0.138 ± 0.034
5.639TrpMet: 5.639 ± 0.16
0.029TrpAsn: 0.029 ± 0.013
0.029TrpPro: 0.029 ± 0.013
0.006TrpGln: 0.006 ± 0.005
0.017TrpArg: 0.017 ± 0.01
0.023TrpSer: 0.023 ± 0.013
0.046TrpThr: 0.046 ± 0.014
0.017TrpVal: 0.017 ± 0.01
0.011TrpTrp: 0.011 ± 0.009
0.052TrpTyr: 0.052 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.184TyrAla: 2.184 ± 0.108
0.287TyrCys: 0.287 ± 0.042
2.38TyrAsp: 2.38 ± 0.126
1.793TyrGlu: 1.793 ± 0.099
2.012TyrPhe: 2.012 ± 0.115
1.546TyrGly: 1.546 ± 0.085
0.649TyrHis: 0.649 ± 0.056
2.989TyrIle: 2.989 ± 0.126
3.742TyrLys: 3.742 ± 0.147
4.581TyrLeu: 4.581 ± 0.168
0.494TyrMet: 0.494 ± 0.05
2.127TyrAsn: 2.127 ± 0.106
0.741TyrPro: 0.741 ± 0.064
2.54TyrGln: 2.54 ± 0.123
1.075TyrArg: 1.075 ± 0.063
2.362TyrSer: 2.362 ± 0.118
1.724TyrThr: 1.724 ± 0.097
1.851TyrVal: 1.851 ± 0.104
0.201TyrTrp: 0.201 ± 0.034
1.54TyrTyr: 1.54 ± 0.114
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1378 proteins (173983 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski