Frankliniella occidentalis (Western flower thrips) (Euthrips occidentalis)
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 20529 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6J1T7W2|A0A6J1T7W2_FRAOC myotubularin-related protein 10-A isoform X1 OS=Frankliniella occidentalis OX=133901 GN=LOC113214529 PE=3 SV=1
MM1 pKa = 7.67 PSLMEE6 pKa = 4.24 LVGEE10 pKa = 4.51 PGAAPGVVVDD20 pKa = 6.24 DD21 pKa = 4.48 EE22 pKa = 4.53 VLDD25 pKa = 3.96 YY26 pKa = 11.32 DD27 pKa = 4.34 ALLNVSANGSLPRR40 pKa = 11.84 NASLQEE46 pKa = 3.89 DD47 pKa = 4.82 VVFDD51 pKa = 3.86 RR52 pKa = 11.84 TDD54 pKa = 2.87 VRR56 pKa = 11.84 ALFIVLYY63 pKa = 9.31 TLVFCCCFFGEE74 pKa = 4.84 SLDD77 pKa = 4.33 CGPPGPRR84 pKa = 11.84 PGPGPAVHH92 pKa = 6.65 FQDD95 pKa = 4.21 QGAPP99 pKa = 3.41
Molecular weight: 10.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.74
IPC2_protein 3.859
IPC_protein 3.795
Toseland 3.592
ProMoST 3.986
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.757
Rodwell 3.63
Grimsley 3.503
Solomon 3.783
Lehninger 3.732
Nozaki 3.935
DTASelect 4.151
Thurlkill 3.668
EMBOSS 3.77
Sillero 3.923
Patrickios 0.769
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.819
Protein with the highest isoelectric point:
>tr|A0A6J1T5S9|A0A6J1T5S9_FRAOC general odorant-binding protein 71 OS=Frankliniella occidentalis OX=133901 GN=LOC113212498 PE=4 SV=1
MM1 pKa = 7.74 AKK3 pKa = 9.94 QNAAAEE9 pKa = 4.57 GNRR12 pKa = 11.84 TLARR16 pKa = 11.84 ASPGPSGPGPRR27 pKa = 11.84 RR28 pKa = 11.84 GPRR31 pKa = 11.84 RR32 pKa = 11.84 AASPGRR38 pKa = 11.84 GRR40 pKa = 11.84 GPGRR44 pKa = 11.84 GEE46 pKa = 3.83 SLGRR50 pKa = 11.84 GASPGRR56 pKa = 11.84 GPGPGRR62 pKa = 11.84 GPGRR66 pKa = 11.84 GASPGRR72 pKa = 11.84 GPAPASHH79 pKa = 6.73 TARR82 pKa = 11.84 HH83 pKa = 5.73 RR84 pKa = 11.84 PVVAPRR90 pKa = 11.84 GSGGLISASRR100 pKa = 11.84 HH101 pKa = 3.93
Molecular weight: 9.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.43
IPC2_protein 10.921
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.223
Grimsley 12.735
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 11.959
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.147
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15639
4890
20529
12625742
34
19827
615.0
68.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.768 ± 0.023
1.966 ± 0.018
5.486 ± 0.014
6.425 ± 0.022
3.206 ± 0.012
6.543 ± 0.026
2.726 ± 0.013
3.981 ± 0.014
5.398 ± 0.022
9.129 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.197 ± 0.008
3.894 ± 0.012
6.387 ± 0.028
4.594 ± 0.022
6.022 ± 0.02
8.769 ± 0.027
5.53 ± 0.018
6.456 ± 0.016
1.066 ± 0.006
2.457 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here