Halobiforma nitratireducens JCM 10879

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Natrialbales; Natrialbaceae; Halobiforma; Halobiforma nitratireducens

Average proteome isoelectric point is 4.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3534 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M0LCS2|M0LCS2_9EURY Polysulfide reductase NrfD (Fragment) OS=Halobiforma nitratireducens JCM 10879 OX=1227454 GN=C446_15383 PE=4 SV=1
MM1 pKa = 6.54TASVALAGCIGGNGNGDD18 pKa = 3.9DD19 pKa = 4.55NGDD22 pKa = 3.74DD23 pKa = 3.51VADD26 pKa = 3.51ISEE29 pKa = 4.13YY30 pKa = 10.55QYY32 pKa = 11.69DD33 pKa = 3.85RR34 pKa = 11.84EE35 pKa = 4.25EE36 pKa = 4.81PDD38 pKa = 4.32DD39 pKa = 4.05EE40 pKa = 4.88DD41 pKa = 6.14RR42 pKa = 11.84SDD44 pKa = 3.78SLEE47 pKa = 3.73FLQPAEE53 pKa = 4.36RR54 pKa = 11.84DD55 pKa = 3.27EE56 pKa = 5.9DD57 pKa = 4.11FDD59 pKa = 4.38PVVSFDD65 pKa = 3.72SYY67 pKa = 11.19SAQVMNLVFDD77 pKa = 5.28NLYY80 pKa = 10.65EE81 pKa = 4.21WDD83 pKa = 4.53DD84 pKa = 3.82EE85 pKa = 4.44MGLEE89 pKa = 4.09PKK91 pKa = 10.05IADD94 pKa = 3.91GMPEE98 pKa = 3.96EE99 pKa = 4.23QDD101 pKa = 3.76DD102 pKa = 4.36GEE104 pKa = 4.71TYY106 pKa = 10.82VFEE109 pKa = 4.48IKK111 pKa = 10.46EE112 pKa = 4.38GIEE115 pKa = 3.77FHH117 pKa = 7.05NGDD120 pKa = 4.21EE121 pKa = 4.23VTAADD126 pKa = 3.78VAHH129 pKa = 6.86SFTAPVEE136 pKa = 4.23EE137 pKa = 4.5EE138 pKa = 3.83TDD140 pKa = 3.54NAASYY145 pKa = 11.83AMIEE149 pKa = 4.17STEE152 pKa = 4.13VIDD155 pKa = 5.15DD156 pKa = 3.56HH157 pKa = 5.6TLEE160 pKa = 4.42VNLEE164 pKa = 4.03HH165 pKa = 7.46PYY167 pKa = 11.31GPFTMVTMGVEE178 pKa = 4.24VVPEE182 pKa = 3.95DD183 pKa = 3.85VRR185 pKa = 11.84TDD187 pKa = 3.37DD188 pKa = 4.55RR189 pKa = 11.84DD190 pKa = 3.64AFNTDD195 pKa = 4.4PIGSGPFQFADD206 pKa = 4.48FQQGEE211 pKa = 4.52YY212 pKa = 10.85VEE214 pKa = 4.38LEE216 pKa = 3.77RR217 pKa = 11.84WDD219 pKa = 5.08DD220 pKa = 3.72YY221 pKa = 11.12WDD223 pKa = 3.88EE224 pKa = 4.03PHH226 pKa = 6.66PHH228 pKa = 6.73IEE230 pKa = 4.2EE231 pKa = 4.01IRR233 pKa = 11.84FEE235 pKa = 4.23AAPDD239 pKa = 3.67DD240 pKa = 4.51ANRR243 pKa = 11.84IAQILAGDD251 pKa = 3.91TDD253 pKa = 4.4VIDD256 pKa = 3.9TVPATEE262 pKa = 3.78WDD264 pKa = 3.98EE265 pKa = 4.79VEE267 pKa = 4.39GADD270 pKa = 4.76DD271 pKa = 4.07VVIHH275 pKa = 6.31GSRR278 pKa = 11.84SPSYY282 pKa = 9.87MYY284 pKa = 10.97LAFNCNEE291 pKa = 4.16GEE293 pKa = 4.33TTNPDD298 pKa = 2.58VRR300 pKa = 11.84RR301 pKa = 11.84AVGHH305 pKa = 5.93SFSMHH310 pKa = 6.6DD311 pKa = 3.96FVNEE315 pKa = 4.01HH316 pKa = 7.03LGPAADD322 pKa = 4.13PLVSPVPEE330 pKa = 3.97ITNDD334 pKa = 3.27SGDD337 pKa = 3.14WDD339 pKa = 4.36FPVDD343 pKa = 3.73DD344 pKa = 4.53WAEE347 pKa = 3.94MMPEE351 pKa = 4.0YY352 pKa = 10.95DD353 pKa = 3.51PDD355 pKa = 3.46QAQEE359 pKa = 4.08LLEE362 pKa = 4.52DD363 pKa = 3.96AGVPEE368 pKa = 4.78DD369 pKa = 3.91WEE371 pKa = 4.19PTIIAPEE378 pKa = 4.35GGPRR382 pKa = 11.84EE383 pKa = 4.1ALAEE387 pKa = 4.42RR388 pKa = 11.84IGSRR392 pKa = 11.84LTEE395 pKa = 3.74IGYY398 pKa = 9.32GADD401 pKa = 3.41VQGMSFATLVDD412 pKa = 4.09TYY414 pKa = 9.2TTGNADD420 pKa = 3.84DD421 pKa = 4.4YY422 pKa = 11.96EE423 pKa = 4.43MYY425 pKa = 11.09LLGWTGGPDD434 pKa = 3.29PDD436 pKa = 3.86AYY438 pKa = 10.57FYY440 pKa = 11.64NLFHH444 pKa = 7.18EE445 pKa = 4.69SQEE448 pKa = 4.47GVGQGHH454 pKa = 6.76FYY456 pKa = 10.71EE457 pKa = 4.91GQGDD461 pKa = 3.89FHH463 pKa = 8.55DD464 pKa = 5.14SIMNARR470 pKa = 11.84QSADD474 pKa = 3.12QDD476 pKa = 3.68DD477 pKa = 3.95RR478 pKa = 11.84RR479 pKa = 11.84EE480 pKa = 3.99LYY482 pKa = 10.06IDD484 pKa = 3.4VTEE487 pKa = 5.15EE488 pKa = 3.54ILDD491 pKa = 3.84YY492 pKa = 10.9MPVLPAYY499 pKa = 10.22SEE501 pKa = 4.58HH502 pKa = 5.6NTMAARR508 pKa = 11.84EE509 pKa = 4.07EE510 pKa = 4.4VKK512 pKa = 10.63DD513 pKa = 3.85LHH515 pKa = 6.5AHH517 pKa = 6.27PAVSFNPRR525 pKa = 11.84IVSNYY530 pKa = 7.25QNTWIDD536 pKa = 3.42EE537 pKa = 4.24

Molecular weight: 60.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M0MRF1|M0MRF1_9EURY 50S ribosomal protein L4 OS=Halobiforma nitratireducens JCM 10879 OX=1227454 GN=rpl4lp PE=3 SV=1
MM1 pKa = 7.69TGTQTRR7 pKa = 11.84ATALLEE13 pKa = 4.11PVRR16 pKa = 11.84DD17 pKa = 3.59RR18 pKa = 11.84VPVVLVPVLLGGVLLLYY35 pKa = 9.69FVVPLAAFFLQTGTVDD51 pKa = 3.68VIGGLGDD58 pKa = 3.69PVTRR62 pKa = 11.84DD63 pKa = 3.8AIATSLLTAPVATAISTVFGVPLAYY88 pKa = 10.34VLSRR92 pKa = 11.84FSFRR96 pKa = 11.84GKK98 pKa = 10.18RR99 pKa = 11.84LVLAAVLVPLVLPPVVGGAVLLSVLGQFTPIGAAAADD136 pKa = 3.8AGVPLTDD143 pKa = 3.13SRR145 pKa = 11.84LGIVLAQTFVAAPFLVITAKK165 pKa = 10.8AGFDD169 pKa = 3.6DD170 pKa = 3.68VDD172 pKa = 3.59PRR174 pKa = 11.84LEE176 pKa = 3.87EE177 pKa = 3.86AARR180 pKa = 11.84TMGYY184 pKa = 10.79GPIATVRR191 pKa = 11.84RR192 pKa = 11.84VSLPLARR199 pKa = 11.84NAIAAGIVLTFVRR212 pKa = 11.84AIGEE216 pKa = 4.27FGATMMVAYY225 pKa = 10.14NPRR228 pKa = 11.84TMPTRR233 pKa = 11.84IWVAFVSRR241 pKa = 11.84GLEE244 pKa = 3.64AVVPIVVALLVITILVVVAVQLLVGTPKK272 pKa = 10.63RR273 pKa = 11.84FF274 pKa = 3.18

Molecular weight: 28.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3534

0

3534

1004006

24

2023

284.1

30.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.431 ± 0.047

0.739 ± 0.014

9.03 ± 0.057

9.542 ± 0.067

3.14 ± 0.029

8.448 ± 0.048

1.99 ± 0.02

4.101 ± 0.031

1.625 ± 0.025

8.746 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.63 ± 0.019

2.171 ± 0.021

4.75 ± 0.027

2.33 ± 0.024

6.667 ± 0.054

5.542 ± 0.042

6.481 ± 0.034

8.882 ± 0.048

1.109 ± 0.018

2.642 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski