Deinococcus marmoris
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4906 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1U7P0H0|A0A1U7P0H0_9DEIO NADH-ubiquinone oxidoreductase chain H OS=Deinococcus marmoris OX=249408 GN=BOO71_0005029 PE=3 SV=1
MM1 pKa = 7.75 RR2 pKa = 11.84 LKK4 pKa = 10.4 FVPVLLAAALGVATAIPIGPSAPIAINANVQDD36 pKa = 3.72 ACEE39 pKa = 3.86 ITAAPSITFSYY50 pKa = 9.85 EE51 pKa = 3.45 AASATAITGGSDD63 pKa = 3.39 VSVHH67 pKa = 6.22 CNQDD71 pKa = 3.09 TTFLAGYY78 pKa = 9.48 WNDD81 pKa = 3.35 TQANADD87 pKa = 3.75 GTVDD91 pKa = 4.9 LIGPGGQVLQVKK103 pKa = 10.29 LDD105 pKa = 3.67 TEE107 pKa = 4.74 FDD109 pKa = 4.62 PIIQSSDD116 pKa = 3.01 ATDD119 pKa = 3.98 GSRR122 pKa = 11.84 LTFGITATAEE132 pKa = 4.09 PGQWTAAVGAYY143 pKa = 9.83 SATFDD148 pKa = 4.73 YY149 pKa = 10.79 FVGWW153 pKa = 3.4
Molecular weight: 15.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.745
IPC_protein 3.706
Toseland 3.49
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.706
Rodwell 3.541
Grimsley 3.401
Solomon 3.694
Lehninger 3.656
Nozaki 3.859
DTASelect 4.113
Thurlkill 3.567
EMBOSS 3.706
Sillero 3.834
Patrickios 0.769
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.765
Protein with the highest isoelectric point:
>tr|A0A1U7P4J9|A0A1U7P4J9_9DEIO GSDH domain-containing protein OS=Deinococcus marmoris OX=249408 GN=BOO71_0000880 PE=4 SV=1
MM1 pKa = 7.58 TSTTEE6 pKa = 4.42 LIVRR10 pKa = 11.84 LCLAVVLGTLLGLDD24 pKa = 3.68 RR25 pKa = 11.84 LRR27 pKa = 11.84 VEE29 pKa = 4.38 PAAGPRR35 pKa = 11.84 THH37 pKa = 7.07 ALVALGSALLLIVSSEE53 pKa = 3.45 GFMRR57 pKa = 11.84 VIQLPHH63 pKa = 5.87 VVLDD67 pKa = 4.03 PSRR70 pKa = 11.84 VAAQVVSGIGFLGAGVILLRR90 pKa = 11.84 RR91 pKa = 11.84 EE92 pKa = 4.0 TEE94 pKa = 4.13 TIRR97 pKa = 11.84 NLTTAASLWVVAAIGLAVGAGLYY120 pKa = 10.11 VGAVVSTVLALLILRR135 pKa = 11.84 GVKK138 pKa = 10.17 RR139 pKa = 11.84 LEE141 pKa = 3.88 QTQKK145 pKa = 10.92 SLTRR149 pKa = 11.84 RR150 pKa = 11.84 HH151 pKa = 5.79 SLTLRR156 pKa = 11.84 FEE158 pKa = 4.41 TGAEE162 pKa = 3.97 GAAEE166 pKa = 4.05 IEE168 pKa = 4.15 RR169 pKa = 11.84 RR170 pKa = 11.84 AVKK173 pKa = 10.37 AGARR177 pKa = 11.84 CRR179 pKa = 11.84 YY180 pKa = 9.5 RR181 pKa = 11.84 RR182 pKa = 11.84 LQLNNKK188 pKa = 8.94 KK189 pKa = 10.72 GYY191 pKa = 10.1 DD192 pKa = 3.53 EE193 pKa = 5.0 YY194 pKa = 11.03 EE195 pKa = 4.37 LEE197 pKa = 4.37 VSHH200 pKa = 7.08 LSTPALMALSRR211 pKa = 11.84 NCRR214 pKa = 11.84 NVPGLLDD221 pKa = 3.72 LSMRR225 pKa = 4.16
Molecular weight: 24.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.516
IPC_protein 10.496
Toseland 10.526
ProMoST 10.57
Dawson 10.643
Bjellqvist 10.423
Wikipedia 10.906
Rodwell 10.687
Grimsley 10.716
Solomon 10.789
Lehninger 10.745
Nozaki 10.54
DTASelect 10.409
Thurlkill 10.54
EMBOSS 10.935
Sillero 10.584
Patrickios 10.409
IPC_peptide 10.789
IPC2_peptide 9.648
IPC2.peptide.svr19 8.719
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4906
0
4906
1434284
33
2604
292.4
31.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.093 ± 0.047
0.617 ± 0.01
5.239 ± 0.029
5.472 ± 0.037
3.221 ± 0.022
9.11 ± 0.037
2.099 ± 0.02
3.898 ± 0.026
2.852 ± 0.03
11.593 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.055 ± 0.015
2.66 ± 0.023
5.631 ± 0.028
3.941 ± 0.025
6.785 ± 0.034
5.347 ± 0.025
5.987 ± 0.031
7.614 ± 0.031
1.404 ± 0.014
2.378 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here