Micromonospora sp. MW-13

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Micromonospora; unclassified Micromonospora

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6184 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3E2YFG5|A0A3E2YFG5_9ACTN Uncharacterized protein OS=Micromonospora sp. MW-13 OX=2094022 GN=C5N14_30425 PE=4 SV=1
MM1 pKa = 7.7TDD3 pKa = 3.06QNFMNFFLEE12 pKa = 4.12QAAPDD17 pKa = 3.77AAVVLACSRR26 pKa = 11.84ACGLPLEE33 pKa = 4.69SEE35 pKa = 4.93AYY37 pKa = 9.64LQEE40 pKa = 4.24GSVGFVQVIPYY51 pKa = 9.99PEE53 pKa = 4.42GFEE56 pKa = 4.04MGLCIIWPPSTPVSLSQAAVAQDD79 pKa = 3.08IARR82 pKa = 11.84EE83 pKa = 3.88LRR85 pKa = 11.84QRR87 pKa = 11.84VLFDD91 pKa = 3.54VEE93 pKa = 4.89DD94 pKa = 3.96PATASGEE101 pKa = 3.96RR102 pKa = 11.84WMLATPDD109 pKa = 3.82GALLTVDD116 pKa = 2.67VWEE119 pKa = 4.63YY120 pKa = 11.54DD121 pKa = 3.57DD122 pKa = 6.09GVGLAQADD130 pKa = 3.64

Molecular weight: 14.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3E2YG33|A0A3E2YG33_9ACTN D-3-phosphoglycerate dehydrogenase OS=Micromonospora sp. MW-13 OX=2094022 GN=serA2 PE=3 SV=1
MM1 pKa = 7.28GSVVKK6 pKa = 10.49KK7 pKa = 9.45RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.48RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.7LLRR22 pKa = 11.84KK23 pKa = 7.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84LGKK33 pKa = 10.04

Molecular weight: 4.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6184

0

6184

2048476

29

9976

331.3

35.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.398 ± 0.053

0.771 ± 0.009

6.044 ± 0.024

4.964 ± 0.029

2.575 ± 0.016

9.714 ± 0.041

2.132 ± 0.017

2.899 ± 0.023

1.524 ± 0.023

10.536 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.532 ± 0.012

1.673 ± 0.018

6.6 ± 0.034

2.664 ± 0.023

8.749 ± 0.037

4.584 ± 0.025

5.932 ± 0.029

9.128 ± 0.03

1.578 ± 0.011

2.003 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski