Devosia limi DSM 17137
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3824 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F5LVP1|A0A0F5LVP1_9RHIZ Flagellar motor protein MotA OS=Devosia limi DSM 17137 OX=1121477 GN=SAMN02745223_01446 PE=4 SV=1
MM1 pKa = 6.6 NTSTIGIAVVLCSLSAALPALAGDD25 pKa = 3.97 VRR27 pKa = 11.84 AQLTPDD33 pKa = 5.08 LIYY36 pKa = 10.7 SHH38 pKa = 6.67 CQAAGVGSQTEE49 pKa = 4.47 STFMLPGGRR58 pKa = 11.84 VTGTVLCTEE67 pKa = 4.75 ADD69 pKa = 3.51 LVAANMMAPTQYY81 pKa = 11.35 GEE83 pKa = 5.59 DD84 pKa = 3.93 EE85 pKa = 5.67 DD86 pKa = 6.74 GDD88 pKa = 4.43 DD89 pKa = 4.79 DD90 pKa = 3.91 NQARR94 pKa = 11.84 RR95 pKa = 11.84 GHH97 pKa = 7.07 DD98 pKa = 3.94 DD99 pKa = 3.21 NHH101 pKa = 7.31 DD102 pKa = 3.69 DD103 pKa = 3.92
Molecular weight: 10.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.751
IPC2_protein 3.923
IPC_protein 3.884
Toseland 3.668
ProMoST 3.999
Dawson 3.897
Bjellqvist 4.113
Wikipedia 3.872
Rodwell 3.719
Grimsley 3.579
Solomon 3.884
Lehninger 3.846
Nozaki 4.024
DTASelect 4.291
Thurlkill 3.745
EMBOSS 3.872
Sillero 4.024
Patrickios 1.939
IPC_peptide 3.884
IPC2_peptide 3.986
IPC2.peptide.svr19 3.933
Protein with the highest isoelectric point:
>tr|A0A0F5LW26|A0A0F5LW26_9RHIZ Uncharacterized protein OS=Devosia limi DSM 17137 OX=1121477 GN=VW29_02130 PE=3 SV=1
MM1 pKa = 7.7 SKK3 pKa = 10.36 RR4 pKa = 11.84 FAYY7 pKa = 9.75 RR8 pKa = 11.84 ATMVALALASLSGPAFAQMQQDD30 pKa = 3.18 RR31 pKa = 11.84 AMAGGNANLAVTTDD45 pKa = 3.07 IVNVRR50 pKa = 11.84 KK51 pKa = 9.48 GAGMRR56 pKa = 11.84 FPVVDD61 pKa = 3.55 VLPRR65 pKa = 11.84 GAQVQIVRR73 pKa = 11.84 CNSDD77 pKa = 3.22 FCLVDD82 pKa = 3.29 QDD84 pKa = 5.19 GPSGWVAQQFLKK96 pKa = 10.87 RR97 pKa = 11.84 LILRR101 pKa = 4.16
Molecular weight: 10.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.346
IPC2_protein 9.428
IPC_protein 10.116
Toseland 10.599
ProMoST 10.818
Dawson 10.687
Bjellqvist 10.394
Wikipedia 10.877
Rodwell 10.833
Grimsley 10.716
Solomon 10.818
Lehninger 10.804
Nozaki 10.613
DTASelect 10.379
Thurlkill 10.599
EMBOSS 11.008
Sillero 10.628
Patrickios 10.672
IPC_peptide 10.833
IPC2_peptide 9.648
IPC2.peptide.svr19 8.736
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3824
0
3824
1172979
40
3450
306.7
33.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.587 ± 0.055
0.681 ± 0.01
5.665 ± 0.034
5.374 ± 0.036
3.831 ± 0.03
8.679 ± 0.041
1.968 ± 0.02
5.68 ± 0.027
3.041 ± 0.03
10.376 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.601 ± 0.02
2.768 ± 0.024
4.988 ± 0.025
3.262 ± 0.023
6.394 ± 0.044
5.434 ± 0.027
5.481 ± 0.032
7.656 ± 0.03
1.297 ± 0.016
2.238 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here