Gordonia phage GRU3
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 26 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K0N694|A0A0K0N694_9CAUD Uncharacterized protein OS=Gordonia phage GRU3 OX=1647473 GN=GRU3_24 PE=4 SV=1
MM1 pKa = 7.14 STLTDD6 pKa = 3.36 ALGRR10 pKa = 11.84 VVDD13 pKa = 4.34 ALTAAGIAATTNPADD28 pKa = 4.04 VIAPGAWVIPHH39 pKa = 7.6 DD40 pKa = 3.68 ITDD43 pKa = 3.67 PTLCGGLTVRR53 pKa = 11.84 ADD55 pKa = 3.6 VCLVAPDD62 pKa = 4.19 NGTANAIAILGDD74 pKa = 4.37 LLDD77 pKa = 3.66 QAAPVLTFDD86 pKa = 3.56 EE87 pKa = 5.23 PVRR90 pKa = 11.84 SMAVTPPGLSALPALVITTTTDD112 pKa = 2.52
Molecular weight: 11.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.207
IPC2_protein 3.668
IPC_protein 3.63
Toseland 3.401
ProMoST 3.77
Dawson 3.668
Bjellqvist 3.91
Wikipedia 3.694
Rodwell 3.465
Grimsley 3.325
Solomon 3.63
Lehninger 3.592
Nozaki 3.808
DTASelect 4.113
Thurlkill 3.503
EMBOSS 3.681
Sillero 3.77
Patrickios 3.465
IPC_peptide 3.617
IPC2_peptide 3.732
IPC2.peptide.svr19 3.789
Protein with the highest isoelectric point:
>tr|A0A0K0N6J0|A0A0K0N6J0_9CAUD Uncharacterized protein OS=Gordonia phage GRU3 OX=1647473 GN=GRU3_22 PE=4 SV=1
MM1 pKa = 7.5 KK2 pKa = 10.24 GATRR6 pKa = 11.84 SRR8 pKa = 11.84 FSPKK12 pKa = 10.27 VIAQQADD19 pKa = 3.71 RR20 pKa = 11.84 AEE22 pKa = 4.97 LDD24 pKa = 3.55 DD25 pKa = 5.08 CSDD28 pKa = 3.29 MFTWSAAIAAFISHH42 pKa = 7.1 LSAGGRR48 pKa = 11.84 ARR50 pKa = 11.84 GTRR53 pKa = 11.84 EE54 pKa = 3.35 LRR56 pKa = 11.84 RR57 pKa = 11.84 YY58 pKa = 9.7 HH59 pKa = 6.82 LLCFAEE65 pKa = 4.59 VVEE68 pKa = 4.58 PLSPLAVTVTDD79 pKa = 4.12 LEE81 pKa = 4.24 AWLGRR86 pKa = 11.84 EE87 pKa = 3.84 DD88 pKa = 2.96 WTANTRR94 pKa = 11.84 RR95 pKa = 11.84 SARR98 pKa = 11.84 STLVGFFSWVSANGWRR114 pKa = 11.84 ADD116 pKa = 3.54 NPAALLATVTGVLGRR131 pKa = 11.84 PRR133 pKa = 11.84 PCPEE137 pKa = 3.32 QFLRR141 pKa = 11.84 SAVIAAGPRR150 pKa = 11.84 EE151 pKa = 4.27 RR152 pKa = 11.84 LMLALGAGCGLRR164 pKa = 11.84 RR165 pKa = 11.84 GEE167 pKa = 4.04 IARR170 pKa = 11.84 VRR172 pKa = 11.84 GDD174 pKa = 3.53 HH175 pKa = 7.09 VEE177 pKa = 4.04 DD178 pKa = 4.96 TIDD181 pKa = 3.87 GPILRR186 pKa = 11.84 VLGKK190 pKa = 10.34 GNKK193 pKa = 7.92 TRR195 pKa = 11.84 TVPISDD201 pKa = 3.82 DD202 pKa = 2.82 MAMRR206 pKa = 11.84 LRR208 pKa = 11.84 EE209 pKa = 3.83 RR210 pKa = 11.84 AGYY213 pKa = 10.08 AFPSPRR219 pKa = 11.84 GGHH222 pKa = 5.94 LTPAHH227 pKa = 5.76 VGKK230 pKa = 10.26 LVSRR234 pKa = 11.84 LLPPGWTTHH243 pKa = 4.72 TLRR246 pKa = 11.84 HH247 pKa = 5.89 RR248 pKa = 11.84 FASAAYY254 pKa = 9.79 RR255 pKa = 11.84 ADD257 pKa = 3.86 RR258 pKa = 11.84 DD259 pKa = 3.24 IRR261 pKa = 11.84 AVQEE265 pKa = 4.27 LLGHH269 pKa = 6.66 ASVATTQIYY278 pKa = 7.84 TAIPDD283 pKa = 3.83 DD284 pKa = 3.59 ATRR287 pKa = 11.84 RR288 pKa = 11.84 ASLAAAIVAA297 pKa = 4.31
Molecular weight: 32.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.34
IPC2_protein 9.428
IPC_protein 10.57
Toseland 10.613
ProMoST 10.555
Dawson 10.701
Bjellqvist 10.526
Wikipedia 11.008
Rodwell 10.628
Grimsley 10.76
Solomon 10.921
Lehninger 10.877
Nozaki 10.628
DTASelect 10.511
Thurlkill 10.628
EMBOSS 11.052
Sillero 10.657
Patrickios 10.335
IPC_peptide 10.935
IPC2_peptide 9.882
IPC2.peptide.svr19 8.72
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
26
0
26
5377
34
656
206.8
22.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.116 ± 0.795
0.763 ± 0.151
6.379 ± 0.609
4.036 ± 0.351
2.808 ± 0.18
8.369 ± 0.328
1.804 ± 0.255
5.133 ± 0.528
2.827 ± 0.26
7.532 ± 0.327
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.176 ± 0.174
3.199 ± 0.374
6.156 ± 0.524
2.548 ± 0.238
6.974 ± 0.725
5.505 ± 0.278
7.104 ± 0.42
8.22 ± 0.559
2.12 ± 0.28
2.232 ± 0.268
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here