Polaribacter gangjinensis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Polaribacter

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2512 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S7WC78|A0A2S7WC78_9FLAO Two-component sensor histidine kinase OS=Polaribacter gangjinensis OX=574710 GN=BTO13_08210 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.39KK3 pKa = 10.39NYY5 pKa = 9.54FLSLFTLLFITVSAFAQDD23 pKa = 3.68PVINEE28 pKa = 4.57LDD30 pKa = 3.7ADD32 pKa = 4.22TPGTDD37 pKa = 2.77TAEE40 pKa = 4.5FIEE43 pKa = 5.14IKK45 pKa = 8.13WTPNTSLTGYY55 pKa = 10.06IVVLYY60 pKa = 10.51NGSNDD65 pKa = 3.13LSYY68 pKa = 10.73YY69 pKa = 10.54TIDD72 pKa = 4.16LSGKK76 pKa = 8.39STDD79 pKa = 3.28ANGFFMIASAGLASGDD95 pKa = 4.88DD96 pKa = 3.44IVIPDD101 pKa = 3.5NTLQNGADD109 pKa = 3.54AVALYY114 pKa = 9.77QAAAGDD120 pKa = 4.37FPNGTAVTSTNLIDD134 pKa = 4.41ALIYY138 pKa = 8.11DD139 pKa = 4.64TNDD142 pKa = 2.9QDD144 pKa = 5.33DD145 pKa = 4.22AGLLSGLGEE154 pKa = 3.97TVQYY158 pKa = 11.34NEE160 pKa = 5.06DD161 pKa = 3.44GGGNKK166 pKa = 9.78DD167 pKa = 3.05NHH169 pKa = 6.51SIQRR173 pKa = 11.84KK174 pKa = 9.58DD175 pKa = 3.37DD176 pKa = 3.18GTYY179 pKa = 10.58AVAIPTYY186 pKa = 9.67RR187 pKa = 11.84AEE189 pKa = 4.35NVFGDD194 pKa = 3.94PCVLNLTTINTTCDD208 pKa = 3.15ANTNGVDD215 pKa = 3.55TYY217 pKa = 9.32TTTIDD222 pKa = 3.56FTGGNTGITYY232 pKa = 8.32TITAKK237 pKa = 10.59DD238 pKa = 3.82GSSADD243 pKa = 3.53VGSIGGDD250 pKa = 3.08NPNSVEE256 pKa = 4.19SGTIIITGVNEE267 pKa = 4.19VVNFTVNVKK276 pKa = 10.34GGAGSSCDD284 pKa = 3.01IDD286 pKa = 4.73RR287 pKa = 11.84SINAPTCLPAATCPAQGAVIITEE310 pKa = 4.22IMQNPNFVGDD320 pKa = 3.69DD321 pKa = 3.13AGEE324 pKa = 3.97YY325 pKa = 10.46FEE327 pKa = 6.23IYY329 pKa = 8.82NTTNTPIDD337 pKa = 3.73LKK339 pKa = 11.13GWIIKK344 pKa = 7.74TASTGSPVEE353 pKa = 4.04HH354 pKa = 6.89VIPTSLILPANGYY367 pKa = 9.69LVLGEE372 pKa = 4.17NADD375 pKa = 3.62INSNGGVTVDD385 pKa = 3.71YY386 pKa = 10.8QYY388 pKa = 11.85DD389 pKa = 3.42AALFLGNSTSTVTIEE404 pKa = 4.25CSQSVFDD411 pKa = 4.22TVTYY415 pKa = 10.9DD416 pKa = 4.58DD417 pKa = 5.0GATFPDD423 pKa = 4.03PSGASMEE430 pKa = 4.3LATNKK435 pKa = 9.98YY436 pKa = 10.33SHH438 pKa = 7.13TDD440 pKa = 3.02NDD442 pKa = 4.07NGINWATATAEE453 pKa = 4.44IITGGDD459 pKa = 2.85KK460 pKa = 9.86GTPGAANSFVLSVIKK475 pKa = 10.89NNIIEE480 pKa = 4.44GFATYY485 pKa = 9.1PNPVISDD492 pKa = 3.48NFTITSNSADD502 pKa = 3.5KK503 pKa = 11.38KK504 pKa = 10.3EE505 pKa = 3.91VTIFNVLGKK514 pKa = 10.11KK515 pKa = 9.77VLSTTFSGTKK525 pKa = 9.96SDD527 pKa = 3.74INVATISSGIYY538 pKa = 8.74ILKK541 pKa = 10.15VSEE544 pKa = 4.17GSKK547 pKa = 10.01MSTSKK552 pKa = 11.17LIIKK556 pKa = 9.74

Molecular weight: 58.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S7WBL6|A0A2S7WBL6_9FLAO Malate dehydrogenase OS=Polaribacter gangjinensis OX=574710 GN=mdh PE=3 SV=1
MM1 pKa = 7.54SVRR4 pKa = 11.84KK5 pKa = 9.68LKK7 pKa = 10.5PITPGQRR14 pKa = 11.84FRR16 pKa = 11.84VVNGFDD22 pKa = 5.49AITTDD27 pKa = 3.6KK28 pKa = 10.83PEE30 pKa = 4.14KK31 pKa = 10.66SLISSKK37 pKa = 10.75KK38 pKa = 7.9STGGRR43 pKa = 11.84NSQGRR48 pKa = 11.84MTTRR52 pKa = 11.84NIGGGHH58 pKa = 4.61KK59 pKa = 9.66QKK61 pKa = 10.66YY62 pKa = 8.25RR63 pKa = 11.84AIDD66 pKa = 3.77FKK68 pKa = 10.9RR69 pKa = 11.84DD70 pKa = 3.24KK71 pKa = 10.39TGIPATVKK79 pKa = 10.04TIEE82 pKa = 4.0YY83 pKa = 9.86DD84 pKa = 3.47PNRR87 pKa = 11.84TAFIALLFYY96 pKa = 11.17ADD98 pKa = 3.45GEE100 pKa = 4.23KK101 pKa = 10.28RR102 pKa = 11.84YY103 pKa = 10.74VIAQNGLEE111 pKa = 4.0VDD113 pKa = 3.61QVIISGSGIAPEE125 pKa = 4.4IGNTLPLSEE134 pKa = 4.99IPLGTTISCIEE145 pKa = 4.03LRR147 pKa = 11.84PGQGAVMARR156 pKa = 11.84SAGSFAQLMARR167 pKa = 11.84DD168 pKa = 3.96GKK170 pKa = 10.52YY171 pKa = 9.12ATVKK175 pKa = 10.42LPSGEE180 pKa = 4.1TRR182 pKa = 11.84LVLLTCMATIGVVSNSDD199 pKa = 3.21HH200 pKa = 6.09QLLVSGKK207 pKa = 8.88AGRR210 pKa = 11.84RR211 pKa = 11.84RR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84VNAVRR224 pKa = 11.84MNPVDD229 pKa = 3.4HH230 pKa = 7.07PMGGGEE236 pKa = 4.08GRR238 pKa = 11.84SSGGHH243 pKa = 4.7PRR245 pKa = 11.84SRR247 pKa = 11.84NGIPAKK253 pKa = 10.28GYY255 pKa = 7.21KK256 pKa = 8.8TRR258 pKa = 11.84SKK260 pKa = 9.63TKK262 pKa = 10.14ASNKK266 pKa = 9.84YY267 pKa = 8.91IVEE270 pKa = 4.08RR271 pKa = 11.84RR272 pKa = 11.84KK273 pKa = 10.24KK274 pKa = 10.1

Molecular weight: 29.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2512

0

2512

883914

38

2858

351.9

39.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.206 ± 0.048

0.675 ± 0.014

5.225 ± 0.029

6.549 ± 0.05

5.806 ± 0.048

6.122 ± 0.047

1.704 ± 0.02

8.615 ± 0.042

8.349 ± 0.064

9.045 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.087 ± 0.024

6.622 ± 0.054

3.243 ± 0.026

3.527 ± 0.028

3.072 ± 0.032

6.517 ± 0.042

5.864 ± 0.051

5.979 ± 0.036

1.012 ± 0.018

3.779 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski