Streptomyces phage Vondra

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M9Z4D8|A0A6M9Z4D8_9CAUD Uncharacterized protein OS=Streptomyces phage Vondra OX=2736273 GN=21 PE=4 SV=1
MM1 pKa = 7.86TYY3 pKa = 9.38TADD6 pKa = 2.92ITTPAGRR13 pKa = 11.84LVCWEE18 pKa = 4.25CNGTGRR24 pKa = 11.84TANPYY29 pKa = 9.93AHH31 pKa = 7.49EE32 pKa = 4.49CTGPLCQGEE41 pKa = 4.41PPVIEE46 pKa = 4.72CTTCDD51 pKa = 3.9GQGHH55 pKa = 6.65TDD57 pKa = 3.13TDD59 pKa = 3.93GFAA62 pKa = 3.99

Molecular weight: 6.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M9Z3R5|A0A6M9Z3R5_9CAUD Uncharacterized protein OS=Streptomyces phage Vondra OX=2736273 GN=46 PE=4 SV=1
MM1 pKa = 7.69RR2 pKa = 11.84ASDD5 pKa = 3.92LVPRR9 pKa = 11.84PVEE12 pKa = 3.93LSDD15 pKa = 3.94GVPITPRR22 pKa = 11.84PVTGCAVCAALMKK35 pKa = 10.0QWRR38 pKa = 11.84QATSPGSPAFDD49 pKa = 4.24PSHH52 pKa = 5.91ATDD55 pKa = 3.65LAVEE59 pKa = 4.65IRR61 pKa = 11.84RR62 pKa = 11.84HH63 pKa = 3.87PHH65 pKa = 4.04QYY67 pKa = 10.15KK68 pKa = 9.83RR69 pKa = 11.84RR70 pKa = 11.84QRR72 pKa = 11.84TRR74 pKa = 2.78

Molecular weight: 8.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

11603

38

1116

203.6

21.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.66 ± 0.498

1.051 ± 0.168

6.645 ± 0.281

5.076 ± 0.25

1.465 ± 0.114

8.265 ± 0.534

2.112 ± 0.216

3.378 ± 0.246

2.396 ± 0.409

8.075 ± 0.295

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.982 ± 0.113

1.594 ± 0.137

6.464 ± 0.504

3.956 ± 0.282

9.205 ± 0.5

4.499 ± 0.261

7.843 ± 0.411

6.197 ± 0.286

1.896 ± 0.166

2.241 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski