Sulfolobus turreted icosahedral virus 1
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 36 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6Q0K9|Q6Q0K9_9VIRU Uncharacterized protein OS=Sulfolobus turreted icosahedral virus 1 OX=269145 GN=B116 PE=1 SV=1
MM1 pKa = 7.26 KK2 pKa = 10.52 VEE4 pKa = 4.21 EE5 pKa = 4.44 DD6 pKa = 3.33 WEE8 pKa = 4.33 EE9 pKa = 3.68 VDD11 pKa = 4.73 EE12 pKa = 4.96 IEE14 pKa = 5.06 EE15 pKa = 4.19 EE16 pKa = 3.97 VDD18 pKa = 4.11 EE19 pKa = 4.48 ICSRR23 pKa = 11.84 VTCEE27 pKa = 3.88 DD28 pKa = 3.93 CVAEE32 pKa = 4.12 ICGDD36 pKa = 3.45 LCEE39 pKa = 4.44 ILCEE43 pKa = 4.05 EE44 pKa = 4.55 EE45 pKa = 3.65 QDD47 pKa = 4.18 LMEE50 pKa = 4.43 EE51 pKa = 4.44 VEE53 pKa = 4.17
Molecular weight: 6.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.227
IPC2_protein 3.376
IPC_protein 3.249
Toseland 3.109
ProMoST 3.401
Dawson 3.236
Bjellqvist 3.465
Wikipedia 3.134
Rodwell 3.109
Grimsley 3.032
Solomon 3.16
Lehninger 3.109
Nozaki 3.376
DTASelect 3.427
Thurlkill 3.16
EMBOSS 3.147
Sillero 3.376
Patrickios 0.006
IPC_peptide 3.16
IPC2_peptide 3.338
IPC2.peptide.svr19 3.606
Protein with the highest isoelectric point:
>tr|Q6Q0J9|Q6Q0J9_9VIRU Uncharacterized protein OS=Sulfolobus turreted icosahedral virus 1 OX=269145 GN=F93 PE=1 SV=1
MM1 pKa = 7.54 PFSVRR6 pKa = 11.84 NKK8 pKa = 8.28 TFPSGASSSLSILSSINEE26 pKa = 4.27 SIKK29 pKa = 10.87 GYY31 pKa = 7.43 NTPSEE36 pKa = 4.12 MSISNLFNISLLSRR50 pKa = 11.84 FFSSLLPFITNTFRR64 pKa = 11.84 IMM66 pKa = 3.57
Molecular weight: 7.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.255
IPC2_protein 9.619
IPC_protein 10.145
Toseland 10.482
ProMoST 10.101
Dawson 10.613
Bjellqvist 10.262
Wikipedia 10.774
Rodwell 10.965
Grimsley 10.657
Solomon 10.701
Lehninger 10.687
Nozaki 10.438
DTASelect 10.262
Thurlkill 10.482
EMBOSS 10.877
Sillero 10.526
Patrickios 10.906
IPC_peptide 10.701
IPC2_peptide 8.931
IPC2.peptide.svr19 8.647
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
36
0
36
5062
51
557
140.6
15.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.709 ± 0.578
0.77 ± 0.246
4.03 ± 0.294
9.305 ± 0.919
4.07 ± 0.357
6.025 ± 0.536
1.6 ± 0.259
9.008 ± 0.5
8.514 ± 0.909
10.016 ± 0.393
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.995 ± 0.251
4.82 ± 0.288
3.872 ± 0.641
4.089 ± 0.327
3.991 ± 0.417
6.717 ± 0.53
4.721 ± 0.434
5.946 ± 0.348
0.751 ± 0.168
4.05 ± 0.447
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here