Youcai mosaic virus (YoMV)
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q66222|CAPSD_YOMV Capsid protein OS=Youcai mosaic virus OX=228578 GN=CP PE=2 SV=3
MM1 pKa = 7.52 VYY3 pKa = 10.67 NITSSNQYY11 pKa = 10.63 QYY13 pKa = 10.8 FAAMWAEE20 pKa = 4.06 PTAMLNQCVSALSQSYY36 pKa = 7.22 QTQAARR42 pKa = 11.84 DD43 pKa = 3.67 TVRR46 pKa = 11.84 QQFSNLLSAIVTPNQRR62 pKa = 11.84 FPEE65 pKa = 4.32 AGYY68 pKa = 10.16 RR69 pKa = 11.84 VYY71 pKa = 10.85 INSAVLKK78 pKa = 9.44 PLYY81 pKa = 9.69 EE82 pKa = 4.29 SLMKK86 pKa = 10.92 SFDD89 pKa = 3.23 TRR91 pKa = 11.84 NRR93 pKa = 11.84 IIEE96 pKa = 4.26 TEE98 pKa = 4.13 EE99 pKa = 3.68 EE100 pKa = 4.31 SRR102 pKa = 11.84 PSASEE107 pKa = 3.81 VANATQRR114 pKa = 11.84 VDD116 pKa = 3.42 DD117 pKa = 3.9 ATVAIRR123 pKa = 11.84 SQIQLLLNEE132 pKa = 4.95 LSNGHH137 pKa = 5.86 GLMNRR142 pKa = 11.84 AEE144 pKa = 4.44 FEE146 pKa = 4.33 VLLPWATAPATT157 pKa = 3.61
Molecular weight: 17.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.063
IPC2_protein 5.258
IPC_protein 5.067
Toseland 4.978
ProMoST 5.156
Dawson 5.016
Bjellqvist 5.156
Wikipedia 4.838
Rodwell 4.94
Grimsley 4.889
Solomon 5.003
Lehninger 4.965
Nozaki 5.13
DTASelect 5.207
Thurlkill 4.965
EMBOSS 4.876
Sillero 5.207
Patrickios 3.859
IPC_peptide 5.016
IPC2_peptide 5.207
IPC2.peptide.svr19 5.247
Protein with the highest isoelectric point:
>sp|Q66222|CAPSD_YOMV Capsid protein OS=Youcai mosaic virus OX=228578 GN=CP PE=2 SV=3
MM1 pKa = 7.94 SYY3 pKa = 10.28 EE4 pKa = 4.08 PKK6 pKa = 10.69 VSDD9 pKa = 4.73 FLALTKK15 pKa = 10.38 KK16 pKa = 10.85 EE17 pKa = 4.14 EE18 pKa = 4.2 ILPKK22 pKa = 10.55 ALTRR26 pKa = 11.84 LKK28 pKa = 9.72 TVSISTKK35 pKa = 10.4 DD36 pKa = 3.46 VISVKK41 pKa = 10.08 EE42 pKa = 4.16 SEE44 pKa = 4.5 SLCDD48 pKa = 3.89 IDD50 pKa = 6.04 LLVNVPLDD58 pKa = 3.17 KK59 pKa = 10.78 YY60 pKa = 10.67 RR61 pKa = 11.84 YY62 pKa = 8.15 VGVLGVVFTGEE73 pKa = 3.77 WLVPDD78 pKa = 4.61 FVKK81 pKa = 10.99 GGVTVSVIDD90 pKa = 4.22 KK91 pKa = 10.23 RR92 pKa = 11.84 LEE94 pKa = 3.82 NSKK97 pKa = 10.48 EE98 pKa = 4.22 CIIGTYY104 pKa = 9.64 RR105 pKa = 11.84 AAAKK109 pKa = 10.17 DD110 pKa = 3.26 RR111 pKa = 11.84 RR112 pKa = 11.84 FQFKK116 pKa = 10.22 LVPNYY121 pKa = 9.44 FVSVADD127 pKa = 4.32 AKK129 pKa = 10.32 RR130 pKa = 11.84 KK131 pKa = 7.58 PWQVHH136 pKa = 4.64 VRR138 pKa = 11.84 IQNLKK143 pKa = 9.94 IEE145 pKa = 5.03 AGWQPLALEE154 pKa = 4.52 VVSVAMVTNNVVVKK168 pKa = 9.49 GLRR171 pKa = 11.84 EE172 pKa = 3.73 KK173 pKa = 10.91 VIAVNDD179 pKa = 4.06 PNVEE183 pKa = 4.0 GFEE186 pKa = 4.26 GVVDD190 pKa = 4.18 DD191 pKa = 5.83 FVDD194 pKa = 3.91 SVAAFKK200 pKa = 10.9 AIDD203 pKa = 3.49 SFRR206 pKa = 11.84 KK207 pKa = 8.15 KK208 pKa = 10.03 KK209 pKa = 10.47 KK210 pKa = 10.04 RR211 pKa = 11.84 IGGRR215 pKa = 11.84 DD216 pKa = 3.46 VNSNKK221 pKa = 9.53 YY222 pKa = 8.94 RR223 pKa = 11.84 YY224 pKa = 9.6 RR225 pKa = 11.84 PEE227 pKa = 5.01 RR228 pKa = 11.84 YY229 pKa = 9.44 AGPDD233 pKa = 2.87 SLQYY237 pKa = 10.63 KK238 pKa = 9.31 EE239 pKa = 4.9 EE240 pKa = 4.37 NGLQHH245 pKa = 6.97 HH246 pKa = 6.38 EE247 pKa = 4.32 LEE249 pKa = 4.52 SVPVFRR255 pKa = 11.84 SDD257 pKa = 2.84 VGRR260 pKa = 11.84 AHH262 pKa = 7.36 SDD264 pKa = 2.78 AA265 pKa = 5.51
Molecular weight: 29.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.534
IPC2_protein 8.419
IPC_protein 8.346
Toseland 9.107
ProMoST 8.873
Dawson 9.37
Bjellqvist 9.107
Wikipedia 9.516
Rodwell 9.663
Grimsley 9.428
Solomon 9.458
Lehninger 9.414
Nozaki 9.238
DTASelect 9.063
Thurlkill 9.238
EMBOSS 9.545
Sillero 9.341
Patrickios 5.003
IPC_peptide 9.443
IPC2_peptide 7.629
IPC2.peptide.svr19 7.646
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
2019
157
1597
673.0
76.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.33 ± 0.884
1.981 ± 0.609
6.241 ± 0.697
6.439 ± 0.207
5.052 ± 0.605
4.408 ± 0.482
2.13 ± 0.456
4.755 ± 0.121
7.083 ± 1.07
9.411 ± 0.692
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.179 ± 0.422
4.26 ± 0.557
3.814 ± 0.216
3.269 ± 0.801
5.25 ± 0.552
6.786 ± 0.58
5.745 ± 0.83
8.866 ± 1.668
1.189 ± 0.019
3.764 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here