Mesorhizobium phage vB_MloP_Lo5R7ANS

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Pairvirus; Mesorhizobium virus Lo5R7ANS

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A076YQK9|A0A076YQK9_9CAUD Uncharacterized protein OS=Mesorhizobium phage vB_MloP_Lo5R7ANS OX=1527771 GN=Lo5R7ANS_61 PE=4 SV=1
MM1 pKa = 7.71NITAQNTFTTPVLIQGGNTFDD22 pKa = 3.69VSVSGTFVATVTLQRR37 pKa = 11.84SKK39 pKa = 11.3DD40 pKa = 3.52GTTNWVDD47 pKa = 2.85VDD49 pKa = 3.88TLTEE53 pKa = 4.43PGEE56 pKa = 4.19WTGDD60 pKa = 3.21AGSAWFYY67 pKa = 11.26RR68 pKa = 11.84LGVKK72 pKa = 9.1TGEE75 pKa = 4.3FTSGTVVVDD84 pKa = 4.64LFDD87 pKa = 3.44

Molecular weight: 9.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A076YJ67|A0A076YJ67_9CAUD Uncharacterized protein OS=Mesorhizobium phage vB_MloP_Lo5R7ANS OX=1527771 GN=Lo5R7ANS_58 PE=4 SV=1
MM1 pKa = 6.59ITIRR5 pKa = 11.84RR6 pKa = 11.84AKK8 pKa = 9.95VADD11 pKa = 5.01AISLAPRR18 pKa = 11.84LRR20 pKa = 11.84SADD23 pKa = 3.7RR24 pKa = 11.84DD25 pKa = 3.31EE26 pKa = 4.23CRR28 pKa = 11.84AYY30 pKa = 10.81LGIEE34 pKa = 4.14PEE36 pKa = 4.3VVLPQLVQQGNYY48 pKa = 6.73VWAGVEE54 pKa = 3.92PNGEE58 pKa = 4.15VFGLFGVDD66 pKa = 3.27PVKK69 pKa = 10.98GNPHH73 pKa = 6.73LGIVWMVSSPAIMKK87 pKa = 9.84HH88 pKa = 4.35RR89 pKa = 11.84RR90 pKa = 11.84EE91 pKa = 4.63LIGMTPKK98 pKa = 9.61WLKK101 pKa = 9.86RR102 pKa = 11.84LHH104 pKa = 6.15KK105 pKa = 10.35VRR107 pKa = 11.84PLLGNHH113 pKa = 5.71IDD115 pKa = 3.8ARR117 pKa = 11.84NTTHH121 pKa = 6.92IRR123 pKa = 11.84WLKK126 pKa = 10.33RR127 pKa = 11.84MGFSFLRR134 pKa = 11.84THH136 pKa = 7.22AEE138 pKa = 3.68FGVEE142 pKa = 3.59KK143 pKa = 10.73RR144 pKa = 11.84PFHH147 pKa = 5.84EE148 pKa = 4.58FARR151 pKa = 11.84LRR153 pKa = 11.84SS154 pKa = 3.66

Molecular weight: 17.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

13813

38

1241

215.8

23.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.367 ± 0.439

0.767 ± 0.11

6.248 ± 0.232

5.567 ± 0.304

3.837 ± 0.173

8.449 ± 0.417

1.803 ± 0.198

5.082 ± 0.15

5.27 ± 0.353

8.347 ± 0.317

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.461 ± 0.163

3.808 ± 0.273

4.539 ± 0.194

3.822 ± 0.215

6.602 ± 0.238

5.683 ± 0.245

6.168 ± 0.296

6.602 ± 0.29

1.585 ± 0.137

2.99 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski