Hubei sobemo-like virus 5

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 5.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEP6|A0A1L3KEP6_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 5 OX=1923238 PE=4 SV=1
MM1 pKa = 7.48VDD3 pKa = 3.37RR4 pKa = 11.84VLFGPWSAADD14 pKa = 3.25TATAMEE20 pKa = 4.39RR21 pKa = 11.84AGKK24 pKa = 10.01SGWSLIPNGFYY35 pKa = 10.96DD36 pKa = 4.37LLAYY40 pKa = 10.45FPDD43 pKa = 4.73DD44 pKa = 4.56VLATDD49 pKa = 4.97CSAFDD54 pKa = 3.34WTYY57 pKa = 9.32PAWVIDD63 pKa = 4.17PIIDD67 pKa = 3.59VKK69 pKa = 10.81LEE71 pKa = 4.11QMGSLPDD78 pKa = 4.27GYY80 pKa = 10.11ATAVRR85 pKa = 11.84TRR87 pKa = 11.84ITEE90 pKa = 4.01VLGVNCTLRR99 pKa = 11.84LPDD102 pKa = 3.82GQRR105 pKa = 11.84LRR107 pKa = 11.84QLVPGIMKK115 pKa = 10.37SGWFLTINLNSDD127 pKa = 3.6AQDD130 pKa = 3.26MLTTLAYY137 pKa = 9.57RR138 pKa = 11.84RR139 pKa = 11.84AYY141 pKa = 10.32GSEE144 pKa = 4.33CPLLWAMGDD153 pKa = 3.71DD154 pKa = 4.38VLMRR158 pKa = 11.84WPQGQDD164 pKa = 2.79SAPLVQQLRR173 pKa = 11.84RR174 pKa = 11.84AGILSKK180 pKa = 10.45FATPSRR186 pKa = 11.84EE187 pKa = 3.64FSGFAVKK194 pKa = 9.37RR195 pKa = 11.84TGSDD199 pKa = 2.59IAVNPLYY206 pKa = 10.04PNKK209 pKa = 10.14HH210 pKa = 6.22KK211 pKa = 10.98YY212 pKa = 10.05LLAHH216 pKa = 6.54TSATDD221 pKa = 3.57LEE223 pKa = 4.42EE224 pKa = 4.64VITSFGMIYY233 pKa = 10.75ALAEE237 pKa = 4.39PDD239 pKa = 3.32VKK241 pKa = 11.0AWLEE245 pKa = 3.78PLLRR249 pKa = 11.84QYY251 pKa = 11.24SRR253 pKa = 11.84WPQSSFRR260 pKa = 11.84AWAHH264 pKa = 5.73GLLSHH269 pKa = 7.24APLLQTGDD277 pKa = 3.51AAGSFGLEE285 pKa = 3.84

Molecular weight: 31.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEP6|A0A1L3KEP6_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 5 OX=1923238 PE=4 SV=1
MM1 pKa = 7.28TPVEE5 pKa = 4.09MFKK8 pKa = 10.8FVIKK12 pKa = 9.9TISDD16 pKa = 3.86FLKK19 pKa = 10.55LLYY22 pKa = 8.98FTAVSMAVLHH32 pKa = 6.04LLIAFSSYY40 pKa = 10.6QFLTSYY46 pKa = 8.99WLCEE50 pKa = 3.94PKK52 pKa = 10.32NRR54 pKa = 11.84SYY56 pKa = 11.52YY57 pKa = 10.61EE58 pKa = 3.91PLNCLGMLTYY68 pKa = 10.05WGFGEE73 pKa = 4.32VLLFATFIAYY83 pKa = 9.84CLLAWFNSPPRR94 pKa = 11.84LTFSPEE100 pKa = 3.39DD101 pKa = 3.53AEE103 pKa = 4.16RR104 pKa = 11.84LEE106 pKa = 4.0RR107 pKa = 11.84DD108 pKa = 3.11RR109 pKa = 11.84ARR111 pKa = 11.84RR112 pKa = 11.84GAYY115 pKa = 9.35GSNEE119 pKa = 3.6ALVRR123 pKa = 11.84EE124 pKa = 4.49FMAWAGTPEE133 pKa = 4.27SYY135 pKa = 10.59KK136 pKa = 10.49EE137 pKa = 3.88DD138 pKa = 3.51SPYY141 pKa = 11.29VSMTPPSWQFSVWRR155 pKa = 11.84AQTEE159 pKa = 4.18AKK161 pKa = 8.29KK162 pKa = 8.9TLIGHH167 pKa = 6.02GCRR170 pKa = 11.84INGHH174 pKa = 6.05LVINYY179 pKa = 8.41HH180 pKa = 5.65VLNTAPLDD188 pKa = 3.72QLYY191 pKa = 10.46LAIIRR196 pKa = 11.84PGKK199 pKa = 7.88EE200 pKa = 3.53TVVQSLATFQFSEE213 pKa = 4.8LLPDD217 pKa = 3.53ICVCPIAQLKK227 pKa = 9.69GIQLTGLKK235 pKa = 8.85EE236 pKa = 4.06AKK238 pKa = 9.53VKK240 pKa = 10.28HH241 pKa = 4.91VQGFQPAYY249 pKa = 9.49IATDD253 pKa = 3.9FPDD256 pKa = 3.93NNASTAAVINHH267 pKa = 6.19PEE269 pKa = 3.66AWGMLQYY276 pKa = 10.68KK277 pKa = 10.42GSTRR281 pKa = 11.84PGFSGATYY289 pKa = 10.36VHH291 pKa = 6.83GSSLFGIHH299 pKa = 5.97CHH301 pKa = 6.75GGIQNVGYY309 pKa = 8.89SASYY313 pKa = 10.17IALKK317 pKa = 10.55LKK319 pKa = 10.74NNEE322 pKa = 3.78SSDD325 pKa = 4.07YY326 pKa = 10.74YY327 pKa = 11.35ALQAMLRR334 pKa = 11.84SSRR337 pKa = 11.84DD338 pKa = 2.59RR339 pKa = 11.84DD340 pKa = 3.73YY341 pKa = 11.46QSQRR345 pKa = 11.84VNPDD349 pKa = 2.49EE350 pKa = 4.47FEE352 pKa = 4.04IRR354 pKa = 11.84FQGRR358 pKa = 11.84YY359 pKa = 9.31FIIEE363 pKa = 3.92TEE365 pKa = 4.44EE366 pKa = 3.99YY367 pKa = 10.63QEE369 pKa = 5.91LEE371 pKa = 4.29DD372 pKa = 4.4NYY374 pKa = 11.44GDD376 pKa = 4.25GSDD379 pKa = 3.4FHH381 pKa = 6.96PRR383 pKa = 11.84SKK385 pKa = 10.64KK386 pKa = 9.44KK387 pKa = 10.29AWRR390 pKa = 11.84NRR392 pKa = 11.84QDD394 pKa = 3.07WEE396 pKa = 4.25NNTAFDD402 pKa = 4.29PMLLPWEE409 pKa = 4.44NNNVSPSSPSQQPPSTTEE427 pKa = 3.25IATQTEE433 pKa = 4.74MEE435 pKa = 4.46WEE437 pKa = 4.08NAPVFDD443 pKa = 5.45DD444 pKa = 4.44GFRR447 pKa = 11.84IQQLTARR454 pKa = 11.84VDD456 pKa = 3.51ALMAQCDD463 pKa = 3.87SEE465 pKa = 4.95RR466 pKa = 11.84EE467 pKa = 3.7QRR469 pKa = 11.84LALRR473 pKa = 11.84QQCEE477 pKa = 4.24RR478 pKa = 11.84EE479 pKa = 4.11WANTTMWIEE488 pKa = 3.99ALNDD492 pKa = 3.58KK493 pKa = 10.46IMRR496 pKa = 11.84LWDD499 pKa = 3.66RR500 pKa = 11.84EE501 pKa = 4.16LGLEE505 pKa = 5.07SEE507 pKa = 5.37DD508 pKa = 3.67EE509 pKa = 4.26DD510 pKa = 5.17DD511 pKa = 4.7PRR513 pKa = 11.84EE514 pKa = 4.09LFPGVVFEE522 pKa = 4.72NADD525 pKa = 3.64PEE527 pKa = 4.42PTVPEE532 pKa = 3.87IEE534 pKa = 3.93ITEE537 pKa = 4.28VVEE540 pKa = 4.63PEE542 pKa = 3.99APNANSPTQNTMGGATSGMQEE563 pKa = 4.39PTSSHH568 pKa = 6.44PPSPNQAHH576 pKa = 7.27LSTSQMDD583 pKa = 3.84SLISGLQQHH592 pKa = 5.59QQQSARR598 pKa = 11.84ALQSIQSILAQQTALLSRR616 pKa = 11.84LTPEE620 pKa = 3.68NHH622 pKa = 6.3SSQEE626 pKa = 4.02RR627 pKa = 11.84GSGSGTAPVTRR638 pKa = 11.84RR639 pKa = 11.84GNRR642 pKa = 11.84WSRR645 pKa = 11.84RR646 pKa = 11.84TRR648 pKa = 11.84RR649 pKa = 11.84PGSRR653 pKa = 11.84QPGASTGPEE662 pKa = 3.96QQASAPSRR670 pKa = 11.84VTQPSRR676 pKa = 11.84TRR678 pKa = 11.84LDD680 pKa = 3.45GTASASPTVRR690 pKa = 11.84TGRR693 pKa = 11.84SSSNSSQPALTPSQRR708 pKa = 11.84AAAAALRR715 pKa = 11.84RR716 pKa = 11.84NPRR719 pKa = 11.84VQPAQQTSSSSS730 pKa = 3.11

Molecular weight: 81.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1015

285

730

507.5

56.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.064 ± 0.758

1.182 ± 0.067

4.828 ± 1.136

6.01 ± 1.297

3.842 ± 0.009

6.108 ± 0.653

1.773 ± 0.192

3.941 ± 0.042

2.66 ± 0.076

9.261 ± 1.566

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.365 ± 0.229

3.842 ± 0.901

6.798 ± 0.25

6.01 ± 1.115

6.601 ± 0.33

9.064 ± 1.061

6.404 ± 0.228

4.532 ± 0.379

2.463 ± 0.542

3.251 ± 0.134
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski