Smittium megazygosporum

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Zoopagomycota; Kickxellomycotina; Harpellomycetes; Harpellales; Legeriomycetaceae; Smittium

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6835 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T9ZD40|A0A2T9ZD40_9FUNG Uncharacterized protein OS=Smittium megazygosporum OX=133381 GN=BB560_003038 PE=4 SV=1
MM1 pKa = 7.75LILFLILVLFGAQNISAQNEE21 pKa = 4.04FSCVNGRR28 pKa = 11.84AVYY31 pKa = 9.06TYY33 pKa = 10.44DD34 pKa = 4.34GNITLSGDD42 pKa = 3.62PNGGTDD48 pKa = 4.97AFIKK52 pKa = 10.12DD53 pKa = 3.97LSMLCDD59 pKa = 3.38SPSFQITVTGYY70 pKa = 10.51GGSDD74 pKa = 3.44PLISLHH80 pKa = 5.84NQASSADD87 pKa = 4.53AIYY90 pKa = 9.9MDD92 pKa = 4.04YY93 pKa = 11.07GFEE96 pKa = 3.88SGYY99 pKa = 11.01YY100 pKa = 8.92SVCIKK105 pKa = 10.7QSDD108 pKa = 3.81SMGACTKK115 pKa = 8.58TQSTKK120 pKa = 10.65YY121 pKa = 9.77VNKK124 pKa = 9.19LTTIYY129 pKa = 11.01VNVNGTIIQMGTVTVKK145 pKa = 10.57GVNTKK150 pKa = 8.23ITQTTFPSGYY160 pKa = 8.54NVDD163 pKa = 4.1YY164 pKa = 10.96IRR166 pKa = 11.84FSVWEE171 pKa = 4.03SLFAYY176 pKa = 9.29TNIQLRR182 pKa = 11.84CDD184 pKa = 4.88DD185 pKa = 3.88ICDD188 pKa = 3.49NSTVSNKK195 pKa = 6.62TWKK198 pKa = 9.9PIPTTSSTTPVLTTSAPTTTATPALTTSAPITTATTLTTPTTPVLTTSDD247 pKa = 4.48SITTANPLNTTTTSITTSVIPTSSSAIISLTTTIGIDD284 pKa = 3.41VSISDD289 pKa = 3.64TAMITAEE296 pKa = 4.18TTFDD300 pKa = 3.17VSTISTGDD308 pKa = 3.28TKK310 pKa = 11.6SNTNLNNITTNTYY323 pKa = 10.25TMVDD327 pKa = 3.34TALTSTIYY335 pKa = 7.9TTTDD339 pKa = 2.71TAAGTDD345 pKa = 3.15RR346 pKa = 11.84GIISTGLTTTEE357 pKa = 3.97TDD359 pKa = 3.19SFSASTGISSADD371 pKa = 3.15TGAPGTDD378 pKa = 2.74TSVARR383 pKa = 11.84TTSGIDD389 pKa = 3.06EE390 pKa = 4.79TNTVDD395 pKa = 2.93TSTISTVLVDD405 pKa = 3.36TSTNYY410 pKa = 10.4SYY412 pKa = 11.32SVSSSTGTNIIISTEE427 pKa = 4.04YY428 pKa = 10.2TEE430 pKa = 5.1SSSTTITSGTGAPDD444 pKa = 2.89ITGFTDD450 pKa = 3.15TNTVADD456 pKa = 3.85ITVSASTEE464 pKa = 3.91AKK466 pKa = 9.02STVSSSTQTLITTGSTGSIGTTDD489 pKa = 3.67SFNGPTDD496 pKa = 3.31TRR498 pKa = 11.84TMSTSATEE506 pKa = 4.06STYY509 pKa = 10.45LTDD512 pKa = 5.22IYY514 pKa = 9.69TQSGNTTEE522 pKa = 5.25SEE524 pKa = 4.4TTNLTNIDD532 pKa = 4.11TEE534 pKa = 4.53STSAGATSLIGATEE548 pKa = 4.35SNNSEE553 pKa = 4.15STGKK557 pKa = 10.92SNTTSLTNGNTEE569 pKa = 4.18STNSSMTEE577 pKa = 3.61EE578 pKa = 4.49TIATSSIGTEE588 pKa = 4.01DD589 pKa = 3.38RR590 pKa = 11.84TTGTGATDD598 pKa = 3.67TGATGTLTGATDD610 pKa = 3.74TGATGTLTGATDD622 pKa = 3.74TGATGTLTT630 pKa = 3.45

Molecular weight: 64.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T9ZAZ1|A0A2T9ZAZ1_9FUNG ABC transporter domain-containing protein OS=Smittium megazygosporum OX=133381 GN=BB560_003791 PE=4 SV=1
MM1 pKa = 6.44TVKK4 pKa = 10.26RR5 pKa = 11.84RR6 pKa = 11.84NHH8 pKa = 4.84GRR10 pKa = 11.84NKK12 pKa = 9.9KK13 pKa = 10.13GRR15 pKa = 11.84GKK17 pKa = 9.21VTFIRR22 pKa = 11.84CDD24 pKa = 3.14NCSRR28 pKa = 11.84CCPKK32 pKa = 10.77DD33 pKa = 3.45KK34 pKa = 10.56AIKK37 pKa = 10.36RR38 pKa = 11.84FTIRR42 pKa = 11.84NMVEE46 pKa = 3.45AAGQRR51 pKa = 11.84DD52 pKa = 3.55MADD55 pKa = 2.72ASVYY59 pKa = 7.35EE60 pKa = 4.57TYY62 pKa = 11.02VLPKK66 pKa = 10.24LYY68 pKa = 10.88LKK70 pKa = 9.37MQYY73 pKa = 10.28CVACAIHH80 pKa = 5.39NHH82 pKa = 4.57IVRR85 pKa = 11.84VRR87 pKa = 11.84STEE90 pKa = 3.48GRR92 pKa = 11.84RR93 pKa = 11.84IRR95 pKa = 11.84TPPPRR100 pKa = 11.84VRR102 pKa = 11.84YY103 pKa = 9.99NKK105 pKa = 9.9DD106 pKa = 2.82GKK108 pKa = 10.61KK109 pKa = 10.0INPAVAAQSAA119 pKa = 3.62

Molecular weight: 13.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6835

0

6835

3127516

49

4877

457.6

51.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.222 ± 0.028

1.21 ± 0.012

5.423 ± 0.019

6.031 ± 0.027

4.753 ± 0.022

4.858 ± 0.028

1.868 ± 0.013

6.841 ± 0.021

7.515 ± 0.029

9.311 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.823 ± 0.011

6.623 ± 0.027

4.646 ± 0.028

3.828 ± 0.02

3.984 ± 0.022

10.888 ± 0.047

5.552 ± 0.028

5.317 ± 0.023

0.819 ± 0.009

3.488 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski