Mycobacterium phage Ekdilam

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TND9|A0A5J6TND9_9CAUD Uncharacterized protein OS=Mycobacterium phage Ekdilam OX=2599862 GN=13 PE=4 SV=1
MM1 pKa = 7.85RR2 pKa = 11.84APSTVEE8 pKa = 4.21VPCPACGEE16 pKa = 4.33PIEE19 pKa = 4.95LSLAFEE25 pKa = 4.49SVDD28 pKa = 3.88APVEE32 pKa = 4.12GLNVASVVVTTVDD45 pKa = 3.24LQDD48 pKa = 3.47RR49 pKa = 11.84VQSHH53 pKa = 6.39GEE55 pKa = 4.0VCPVLAGGGRR65 pKa = 11.84DD66 pKa = 3.55GG67 pKa = 4.83

Molecular weight: 6.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TNG9|A0A5J6TNG9_9CAUD Cro protein OS=Mycobacterium phage Ekdilam OX=2599862 GN=43 PE=4 SV=1
MM1 pKa = 6.98MRR3 pKa = 11.84CADD6 pKa = 4.53RR7 pKa = 11.84AWLALGAGVVAYY19 pKa = 9.05EE20 pKa = 4.06VAAPRR25 pKa = 11.84GEE27 pKa = 4.07LLSEE31 pKa = 4.22GVDD34 pKa = 3.11RR35 pKa = 11.84YY36 pKa = 8.79LTRR39 pKa = 11.84RR40 pKa = 11.84KK41 pKa = 6.54WTTRR45 pKa = 11.84VVVVGLAAHH54 pKa = 7.46LLNLIPQRR62 pKa = 11.84FDD64 pKa = 3.23PLTRR68 pKa = 11.84LAQATRR74 pKa = 11.84GSS76 pKa = 3.56

Molecular weight: 8.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

92

0

92

19075

32

1396

207.3

22.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.117 ± 0.473

1.211 ± 0.167

6.752 ± 0.238

5.531 ± 0.286

2.569 ± 0.151

8.828 ± 0.509

2.139 ± 0.227

3.565 ± 0.182

3.481 ± 0.218

8.55 ± 0.263

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.092 ± 0.098

2.716 ± 0.196

5.573 ± 0.242

3.256 ± 0.163

7.104 ± 0.379

5.232 ± 0.221

5.767 ± 0.206

8.194 ± 0.262

2.071 ± 0.113

2.254 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski