Podoviridae sp. ctKoA10
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5Q2WA12|A0A5Q2WA12_9CAUD Uncharacterized protein OS=Podoviridae sp. ctKoA10 OX=2656709 PE=4 SV=1
MM1 pKa = 6.92 ITKK4 pKa = 10.5 GDD6 pKa = 4.25 LIKK9 pKa = 11.03 GAMQHH14 pKa = 6.16 LAVDD18 pKa = 4.48 GLLMQPMAQDD28 pKa = 3.34 NQSALQHH35 pKa = 6.64 LDD37 pKa = 3.81 DD38 pKa = 5.25 FAATYY43 pKa = 10.51 SAIGQDD49 pKa = 2.91 IGYY52 pKa = 8.19 LQPLEE57 pKa = 4.52 YY58 pKa = 9.38 GTSTEE63 pKa = 4.19 TDD65 pKa = 3.18 DD66 pKa = 4.91 SGVDD70 pKa = 3.5 VSLVGPIKK78 pKa = 10.72 VLLAGYY84 pKa = 8.72 IANMFGKK91 pKa = 9.77 EE92 pKa = 4.0 LNPNKK97 pKa = 10.39 LSWAEE102 pKa = 3.81 NMMMRR107 pKa = 11.84 QLTTVEE113 pKa = 4.15 SCSYY117 pKa = 10.55 PVTLPVGSGNYY128 pKa = 9.18 EE129 pKa = 3.92 AMDD132 pKa = 3.79 DD133 pKa = 3.58 QQYY136 pKa = 10.36 YY137 pKa = 10.38 RR138 pKa = 11.84 GGLPFEE144 pKa = 4.48 NN145 pKa = 4.3
Molecular weight: 15.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.874
IPC2_protein 4.139
IPC_protein 4.075
Toseland 3.884
ProMoST 4.24
Dawson 4.062
Bjellqvist 4.215
Wikipedia 3.999
Rodwell 3.91
Grimsley 3.795
Solomon 4.05
Lehninger 4.012
Nozaki 4.177
DTASelect 4.406
Thurlkill 3.923
EMBOSS 3.999
Sillero 4.202
Patrickios 0.998
IPC_peptide 4.05
IPC2_peptide 4.177
IPC2.peptide.svr19 4.075
Protein with the highest isoelectric point:
>tr|A0A5Q2WAG1|A0A5Q2WAG1_9CAUD D-alanyl-D-alanine carboxypeptidase OS=Podoviridae sp. ctKoA10 OX=2656709 PE=4 SV=1
MM1 pKa = 7.17 TLNRR5 pKa = 11.84 QKK7 pKa = 11.32 LDD9 pKa = 2.93 IGFSDD14 pKa = 3.3 RR15 pKa = 11.84 HH16 pKa = 5.52 NFGEE20 pKa = 4.43 AVRR23 pKa = 11.84 QRR25 pKa = 11.84 LTVGSNNQQGAFKK38 pKa = 10.82
Molecular weight: 4.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.297
IPC2_protein 9.531
IPC_protein 10.394
Toseland 11.052
ProMoST 11.125
Dawson 11.067
Bjellqvist 10.833
Wikipedia 11.345
Rodwell 11.199
Grimsley 11.096
Solomon 11.345
Lehninger 11.301
Nozaki 11.023
DTASelect 10.833
Thurlkill 11.023
EMBOSS 11.477
Sillero 11.023
Patrickios 11.082
IPC_peptide 11.359
IPC2_peptide 9.736
IPC2.peptide.svr19 9.053
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
50
0
50
10255
29
700
205.1
22.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.122 ± 0.516
1.112 ± 0.195
5.792 ± 0.257
5.802 ± 0.463
3.686 ± 0.216
7.557 ± 0.517
1.482 ± 0.2
5.617 ± 0.324
5.89 ± 0.444
8.133 ± 0.329
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.506 ± 0.205
4.378 ± 0.3
3.618 ± 0.215
5.266 ± 0.411
4.281 ± 0.221
6.894 ± 0.385
6.348 ± 0.397
6.533 ± 0.304
1.385 ± 0.15
3.598 ± 0.229
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here