Phocoena phocoena papillomavirus 1
Average proteome isoelectric point is 5.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F2VIQ8|F2VIQ8_PSPV Regulatory protein E2 OS=Phocoena phocoena papillomavirus 1 OX=706525 GN=E2 PE=3 SV=1
MM1 pKa = 8.2 DD2 pKa = 4.19 NTQGTDD8 pKa = 3.38 PLEE11 pKa = 4.91 GGSGMDD17 pKa = 3.6 GGWVLLEE24 pKa = 4.19 ATDD27 pKa = 3.56 VDD29 pKa = 4.62 GAGGDD34 pKa = 3.75 DD35 pKa = 6.2 DD36 pKa = 5.17 EE37 pKa = 7.61 DD38 pKa = 4.07 EE39 pKa = 5.39 DD40 pKa = 4.3 EE41 pKa = 6.29 DD42 pKa = 4.19 YY43 pKa = 11.91 GEE45 pKa = 5.4 DD46 pKa = 3.51 FTDD49 pKa = 6.7 FIDD52 pKa = 4.38 DD53 pKa = 3.79 CAKK56 pKa = 10.03 EE57 pKa = 4.25 FSEE60 pKa = 4.49 EE61 pKa = 4.02 THH63 pKa = 6.24 TSLFSQQQIEE73 pKa = 4.12 EE74 pKa = 4.31 DD75 pKa = 3.7 DD76 pKa = 4.05 RR77 pKa = 11.84 AVQALKK83 pKa = 10.59 RR84 pKa = 11.84 KK85 pKa = 9.09 FLEE88 pKa = 4.35 SPKK91 pKa = 10.78 SKK93 pKa = 10.49 VDD95 pKa = 3.6 SEE97 pKa = 4.24 LSPRR101 pKa = 11.84 LAAISLVQRR110 pKa = 11.84 TGKK113 pKa = 10.06 AKK115 pKa = 10.03 KK116 pKa = 9.77 RR117 pKa = 11.84 LYY119 pKa = 10.32 RR120 pKa = 11.84 DD121 pKa = 3.21 QTQDD125 pKa = 3.05 SGHH128 pKa = 6.24 GNSEE132 pKa = 3.81 EE133 pKa = 4.16 SGATEE138 pKa = 4.03 VVVSTQVQKK147 pKa = 11.41 GGLQRR152 pKa = 11.84 VGQTPVVVRR161 pKa = 11.84 ASTQQAAEE169 pKa = 4.22 SAGSQEE175 pKa = 5.17 DD176 pKa = 3.72 YY177 pKa = 10.4 TGRR180 pKa = 11.84 VTQLLRR186 pKa = 11.84 SAQPRR191 pKa = 11.84 CALLGIFKK199 pKa = 10.44 EE200 pKa = 4.33 LFACSFMDD208 pKa = 3.73 LTRR211 pKa = 11.84 CFKK214 pKa = 10.76 SDD216 pKa = 3.25 STAGEE221 pKa = 4.01 DD222 pKa = 3.42 WVCFVGGVPCSLADD236 pKa = 3.75 GVVDD240 pKa = 5.41 LMAPHH245 pKa = 6.54 VLYY248 pKa = 11.1 SHH250 pKa = 6.82 ITSSTCSLGIAVLMLVRR267 pKa = 11.84 WKK269 pKa = 8.92 TAKK272 pKa = 9.32 TRR274 pKa = 11.84 ATVKK278 pKa = 10.56 KK279 pKa = 10.59 LLGSLLAVEE288 pKa = 4.86 SGQMLLEE295 pKa = 4.4 PPRR298 pKa = 11.84 LRR300 pKa = 11.84 NPAAAMFWYY309 pKa = 9.49 KK310 pKa = 10.81 KK311 pKa = 10.5 AMCNSTVVTGEE322 pKa = 3.93 TPQWILRR329 pKa = 11.84 EE330 pKa = 3.98 VSIQDD335 pKa = 3.49 QVGEE339 pKa = 4.0 QCLFSLSDD347 pKa = 3.67 MVQWAYY353 pKa = 11.28 DD354 pKa = 3.48 NGIEE358 pKa = 4.23 TEE360 pKa = 4.07 SAAAYY365 pKa = 9.04 EE366 pKa = 4.25 YY367 pKa = 11.58 ALLADD372 pKa = 4.03 EE373 pKa = 5.67 DD374 pKa = 4.38 KK375 pKa = 11.52 NADD378 pKa = 3.1 AFLRR382 pKa = 11.84 SNQQAKK388 pKa = 8.18 WVGDD392 pKa = 3.59 CMRR395 pKa = 11.84 MVRR398 pKa = 11.84 LYY400 pKa = 10.71 RR401 pKa = 11.84 RR402 pKa = 11.84 AEE404 pKa = 4.03 MNKK407 pKa = 7.28 MTMGQWIKK415 pKa = 10.45 HH416 pKa = 5.29 RR417 pKa = 11.84 SEE419 pKa = 3.94 KK420 pKa = 9.39 THH422 pKa = 7.36 GEE424 pKa = 4.34 GNWKK428 pKa = 10.38 EE429 pKa = 3.63 IFKK432 pKa = 10.4 FLKK435 pKa = 10.04 FQGIEE440 pKa = 3.59 ITSFLTYY447 pKa = 10.17 FRR449 pKa = 11.84 LFLKK453 pKa = 10.23 GVPKK457 pKa = 10.31 KK458 pKa = 10.72 NCIAICGPPNTGKK471 pKa = 10.57 SLFGMSLIDD480 pKa = 4.62 FIDD483 pKa = 3.73 GRR485 pKa = 11.84 VISHH489 pKa = 6.72 CNSNSHH495 pKa = 6.72 FWLQPLTEE503 pKa = 4.77 CKK505 pKa = 10.13 LALLDD510 pKa = 5.16 DD511 pKa = 4.53 ATPPTWDD518 pKa = 3.48 YY519 pKa = 11.59 FDD521 pKa = 3.58 TYY523 pKa = 11.17 LRR525 pKa = 11.84 NLADD529 pKa = 4.28 GNPVSVDD536 pKa = 3.3 TKK538 pKa = 10.81 HH539 pKa = 6.94 RR540 pKa = 11.84 APTQMKK546 pKa = 9.94 CPPLLITTNTDD557 pKa = 2.23 ISQGDD562 pKa = 3.07 RR563 pKa = 11.84 WTYY566 pKa = 9.23 LKK568 pKa = 10.91 SRR570 pKa = 11.84 IKK572 pKa = 10.89 VFTFPEE578 pKa = 4.07 QMPLTEE584 pKa = 4.44 MGDD587 pKa = 3.42 PAFALTRR594 pKa = 11.84 QNWKK598 pKa = 10.67 SFFQRR603 pKa = 11.84 CWSSLGLEE611 pKa = 4.51 DD612 pKa = 5.6 PEE614 pKa = 4.28 QEE616 pKa = 4.56 AANGDD621 pKa = 3.87 PRR623 pKa = 11.84 EE624 pKa = 4.0 VLQPLRR630 pKa = 11.84 CTARR634 pKa = 11.84 RR635 pKa = 11.84 ADD637 pKa = 3.65 GLSS640 pKa = 3.1
Molecular weight: 71.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.043
IPC2_protein 5.08
IPC_protein 5.041
Toseland 4.94
ProMoST 5.169
Dawson 5.016
Bjellqvist 5.156
Wikipedia 4.902
Rodwell 4.927
Grimsley 4.863
Solomon 5.016
Lehninger 4.978
Nozaki 5.143
DTASelect 5.308
Thurlkill 4.952
EMBOSS 4.94
Sillero 5.207
Patrickios 4.126
IPC_peptide 5.029
IPC2_peptide 5.194
IPC2.peptide.svr19 5.206
Protein with the highest isoelectric point:
>tr|F2VIR0|F2VIR0_PSPV Minor capsid protein L2 OS=Phocoena phocoena papillomavirus 1 OX=706525 GN=L2 PE=3 SV=1
LL1 pKa = 7.21 LHH3 pKa = 6.89 LCLAPRR9 pKa = 11.84 AVTSPLYY16 pKa = 10.43 QLLEE20 pKa = 3.9 EE21 pKa = 4.39 TRR23 pKa = 11.84 RR24 pKa = 11.84 TRR26 pKa = 11.84 QRR28 pKa = 11.84 TSEE31 pKa = 4.02 GHH33 pKa = 6.1 PKK35 pKa = 10.23 SGQGPMSAGILSQTQTQDD53 pKa = 3.11 QSPTPQHH60 pKa = 6.51 HH61 pKa = 5.97 PHH63 pKa = 6.89 ILCTPPRR70 pKa = 11.84 SPTSSGSAATEE81 pKa = 4.06 LTSPPPVSVSLTVGVPGAPEE101 pKa = 3.57 ITLSFLLSS109 pKa = 3.46
Molecular weight: 11.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.175
IPC2_protein 7.073
IPC_protein 7.556
Toseland 7.293
ProMoST 7.556
Dawson 7.995
Bjellqvist 8.097
Wikipedia 7.922
Rodwell 7.936
Grimsley 7.307
Solomon 8.273
Lehninger 8.302
Nozaki 8.317
DTASelect 8.083
Thurlkill 8.112
EMBOSS 8.273
Sillero 8.404
Patrickios 4.596
IPC_peptide 8.258
IPC2_peptide 8.039
IPC2.peptide.svr19 7.801
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2425
109
640
404.2
44.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.309 ± 0.429
2.474 ± 0.614
6.68 ± 0.461
5.526 ± 0.453
4.289 ± 0.339
7.918 ± 0.65
2.557 ± 0.405
3.546 ± 0.259
4.619 ± 0.619
8.412 ± 0.505
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.897 ± 0.317
2.763 ± 0.716
6.969 ± 0.98
3.835 ± 0.588
5.567 ± 0.212
8.742 ± 0.789
7.052 ± 0.423
6.639 ± 0.482
1.649 ± 0.353
2.557 ± 0.397
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here