Pepper leaf curl Lahore virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G9FJA9|G9FJA9_9GEMI C4 protein OS=Pepper leaf curl Lahore virus OX=884610 GN=AC4 PE=3 SV=1
MM1 pKa = 7.57PRR3 pKa = 11.84LNSFCVNAKK12 pKa = 10.44NIFLTYY18 pKa = 8.78PKK20 pKa = 10.44CPIPKK25 pKa = 9.32EE26 pKa = 3.87QMLEE30 pKa = 3.65ILQSINCPSDD40 pKa = 3.1KK41 pKa = 10.94LFIRR45 pKa = 11.84VAQEE49 pKa = 3.46KK50 pKa = 9.49HH51 pKa = 5.19QDD53 pKa = 3.38GSLHH57 pKa = 5.16IHH59 pKa = 6.91ALIQFKK65 pKa = 10.72GKK67 pKa = 10.47SKK69 pKa = 10.22FRR71 pKa = 11.84NPRR74 pKa = 11.84HH75 pKa = 6.17FDD77 pKa = 3.03VTHH80 pKa = 7.16PNNSTQFHH88 pKa = 7.21PNFQGAKK95 pKa = 9.08SSSDD99 pKa = 3.06VKK101 pKa = 11.23SYY103 pKa = 10.83IEE105 pKa = 4.63KK106 pKa = 10.79DD107 pKa = 2.7SDD109 pKa = 4.11YY110 pKa = 11.07IDD112 pKa = 3.27WGQFQIDD119 pKa = 3.44GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQTANDD132 pKa = 3.91AAAEE136 pKa = 3.92ALNAGSAEE144 pKa = 3.87AALAIIRR151 pKa = 11.84EE152 pKa = 4.23KK153 pKa = 10.87LPKK156 pKa = 10.46DD157 pKa = 4.77FIFQYY162 pKa = 11.21HH163 pKa = 5.5NLKK166 pKa = 10.79CNLDD170 pKa = 4.1RR171 pKa = 11.84IFTPPVEE178 pKa = 4.6VYY180 pKa = 10.33VSPFLSSSFDD190 pKa = 3.44QVPEE194 pKa = 4.0EE195 pKa = 4.1LEE197 pKa = 3.76EE198 pKa = 4.06WAVEE202 pKa = 4.1NVMVAAARR210 pKa = 11.84PLRR213 pKa = 11.84PMSIVIEE220 pKa = 4.37GDD222 pKa = 3.13SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 3.81LSPRR249 pKa = 11.84VYY251 pKa = 10.92RR252 pKa = 11.84NDD254 pKa = 2.33AWYY257 pKa = 10.88NVIDD261 pKa = 5.2DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.6YY287 pKa = 10.57GKK289 pKa = 9.12PVQIKK294 pKa = 10.41GGIPTIFLCDD304 pKa = 3.98PGPNSSYY311 pKa = 11.3KK312 pKa = 10.63EE313 pKa = 3.91FLDD316 pKa = 3.98EE317 pKa = 4.98PKK319 pKa = 9.68NTALKK324 pKa = 10.38AWAIRR329 pKa = 11.84NATFISLDD337 pKa = 3.39GPLYY341 pKa = 10.81SSADD345 pKa = 3.37QGSTQGSQEE354 pKa = 4.02EE355 pKa = 4.6EE356 pKa = 4.12QTSSCC361 pKa = 4.73

Molecular weight: 40.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G9FJA6|G9FJA6_9GEMI Replication enhancer OS=Pepper leaf curl Lahore virus OX=884610 GN=AC3 PE=3 SV=2
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.72IIISTTASKK16 pKa = 10.44VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 2.99SPYY27 pKa = 10.47ASRR30 pKa = 11.84AAAPTVRR37 pKa = 11.84VTKK40 pKa = 10.57ARR42 pKa = 11.84AWVNRR47 pKa = 11.84PMNRR51 pKa = 11.84KK52 pKa = 7.74PRR54 pKa = 11.84MYY56 pKa = 10.68RR57 pKa = 11.84MYY59 pKa = 10.44RR60 pKa = 11.84SPDD63 pKa = 3.21VPRR66 pKa = 11.84GCEE69 pKa = 4.34GPCKK73 pKa = 10.01VQSFEE78 pKa = 4.02SRR80 pKa = 11.84HH81 pKa = 5.54DD82 pKa = 3.51VVHH85 pKa = 6.75IGKK88 pKa = 9.41VMCISDD94 pKa = 3.67VTRR97 pKa = 11.84GTGLTHH103 pKa = 7.09RR104 pKa = 11.84VGKK107 pKa = 9.66RR108 pKa = 11.84FCVKK112 pKa = 9.94SVYY115 pKa = 10.52VLGKK119 pKa = 9.41IWMDD123 pKa = 3.35EE124 pKa = 4.04NIKK127 pKa = 9.32TKK129 pKa = 10.6NHH131 pKa = 5.78TNSVMFFLVRR141 pKa = 11.84DD142 pKa = 3.84RR143 pKa = 11.84RR144 pKa = 11.84PVDD147 pKa = 3.13KK148 pKa = 10.28PQDD151 pKa = 3.56FGEE154 pKa = 4.33VFNMFDD160 pKa = 4.29NEE162 pKa = 4.11PSTATVKK169 pKa = 10.61NMHH172 pKa = 7.0RR173 pKa = 11.84DD174 pKa = 3.31RR175 pKa = 11.84YY176 pKa = 8.78QVLRR180 pKa = 11.84KK181 pKa = 8.43WHH183 pKa = 5.58ATVTGGQYY191 pKa = 10.85ASKK194 pKa = 10.24EE195 pKa = 3.73QALVKK200 pKa = 10.68KK201 pKa = 9.39FVRR204 pKa = 11.84VNNYY208 pKa = 7.82VVYY211 pKa = 9.96NQQEE215 pKa = 3.8AGKK218 pKa = 10.1YY219 pKa = 8.23EE220 pKa = 4.02NHH222 pKa = 6.47TEE224 pKa = 3.99NALMLYY230 pKa = 7.52MACTHH235 pKa = 7.06ASNPVYY241 pKa = 9.86ATLKK245 pKa = 9.47IRR247 pKa = 11.84IYY249 pKa = 10.67FYY251 pKa = 11.26DD252 pKa = 3.51SVSNN256 pKa = 3.9

Molecular weight: 29.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1091

85

361

181.8

20.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.683 ± 0.817

2.383 ± 0.633

5.225 ± 0.524

4.583 ± 0.542

4.033 ± 0.51

4.95 ± 0.481

3.758 ± 0.678

4.583 ± 0.917

5.225 ± 0.931

7.058 ± 1.198

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.208 ± 0.704

5.133 ± 0.612

6.416 ± 0.87

5.133 ± 0.985

7.149 ± 1.038

8.433 ± 1.523

5.408 ± 0.741

6.874 ± 1.366

1.192 ± 0.201

3.575 ± 0.631
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski