bacterium HR16

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3294 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5X5P5|A0A2H5X5P5_9BACT Uncharacterized protein OS=bacterium HR16 OX=2035411 GN=HRbin16_02315 PE=4 SV=1
MM1 pKa = 7.36WAFFAYY7 pKa = 10.46LIAATAFYY15 pKa = 9.56TILVMIAQPDD25 pKa = 4.35PYY27 pKa = 10.93DD28 pKa = 3.89SEE30 pKa = 4.26AWEE33 pKa = 4.3MPDD36 pKa = 5.38DD37 pKa = 4.13VEE39 pKa = 5.53LPIPTAEE46 pKa = 4.35EE47 pKa = 3.73EE48 pKa = 4.31VEE50 pKa = 4.49TYY52 pKa = 10.53RR53 pKa = 11.84KK54 pKa = 9.65AAA56 pKa = 3.7

Molecular weight: 6.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5X7P2|A0A2H5X7P2_9BACT NTP_transf_2 domain-containing protein OS=bacterium HR16 OX=2035411 GN=HRbin16_02947 PE=4 SV=1
MM1 pKa = 7.44NGQPRR6 pKa = 11.84VIVFSTPNCPYY17 pKa = 10.66CNMAKK22 pKa = 10.18RR23 pKa = 11.84YY24 pKa = 9.21LRR26 pKa = 11.84EE27 pKa = 3.45RR28 pKa = 11.84GIRR31 pKa = 11.84FRR33 pKa = 11.84DD34 pKa = 3.19VDD36 pKa = 3.75VSRR39 pKa = 11.84DD40 pKa = 3.24PAAARR45 pKa = 11.84DD46 pKa = 3.67MVRR49 pKa = 11.84RR50 pKa = 11.84SGQQGVPVIDD60 pKa = 3.52INGKK64 pKa = 9.22IVVGFDD70 pKa = 2.95RR71 pKa = 11.84AKK73 pKa = 10.36INQLLGLKK81 pKa = 9.88

Molecular weight: 9.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3294

0

3294

1088270

29

3688

330.4

36.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.529 ± 0.046

1.059 ± 0.017

4.785 ± 0.026

6.617 ± 0.059

3.559 ± 0.026

7.751 ± 0.048

2.307 ± 0.026

5.004 ± 0.031

3.166 ± 0.034

10.154 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.33 ± 0.021

2.82 ± 0.029

5.487 ± 0.031

4.242 ± 0.03

7.615 ± 0.041

4.968 ± 0.032

5.299 ± 0.038

8.324 ± 0.041

1.886 ± 0.026

3.1 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski