Bifidobacterium commune
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1238 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1C4H5B3|A0A1C4H5B3_9BIFI NAD(+) diphosphatase OS=Bifidobacterium commune OX=1505727 GN=GA0061077_0913 PE=4 SV=1
MM1 pKa = 7.8 AYY3 pKa = 10.28 VIAQPCIDD11 pKa = 4.47 VKK13 pKa = 11.31 DD14 pKa = 3.83 KK15 pKa = 11.59 ACVDD19 pKa = 4.0 EE20 pKa = 5.6 CPVDD24 pKa = 4.76 CIYY27 pKa = 11.04 EE28 pKa = 4.31 GNRR31 pKa = 11.84 TLYY34 pKa = 10.27 INPNEE39 pKa = 4.32 CVDD42 pKa = 4.29 CGACEE47 pKa = 3.97 PVCPTEE53 pKa = 4.64 AIFYY57 pKa = 10.04 EE58 pKa = 4.75 DD59 pKa = 4.8 DD60 pKa = 5.52 VPDD63 pKa = 3.22 EE64 pKa = 4.13 WAWYY68 pKa = 9.6 KK69 pKa = 10.86 DD70 pKa = 3.21 AAVTYY75 pKa = 10.42 FNDD78 pKa = 4.05 LGDD81 pKa = 3.87 LGGAQVAGPSGKK93 pKa = 10.17 DD94 pKa = 3.11 EE95 pKa = 4.15 GRR97 pKa = 11.84 IAALPPQVV105 pKa = 3.42
Molecular weight: 11.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.884
IPC2_protein 3.732
IPC_protein 3.694
Toseland 3.49
ProMoST 3.884
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.401
Solomon 3.681
Lehninger 3.63
Nozaki 3.821
DTASelect 4.037
Thurlkill 3.554
EMBOSS 3.656
Sillero 3.821
Patrickios 0.006
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.757
Protein with the highest isoelectric point:
>tr|A0A1C4H6M6|A0A1C4H6M6_9BIFI 8-oxo-dGTP pyrophosphatase MutT NUDIX family OS=Bifidobacterium commune OX=1505727 GN=GA0061077_1296 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 HH13 pKa = 4.79 MKK15 pKa = 9.36 HH16 pKa = 5.81 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MRR23 pKa = 11.84 TRR25 pKa = 11.84 AGRR28 pKa = 11.84 AVINRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.86 GRR39 pKa = 11.84 KK40 pKa = 8.63 NLTVV44 pKa = 3.1
Molecular weight: 5.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1238
0
1238
459946
36
3134
371.5
40.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.901 ± 0.063
0.938 ± 0.022
6.567 ± 0.062
5.812 ± 0.078
3.376 ± 0.046
7.973 ± 0.058
2.213 ± 0.029
5.502 ± 0.051
4.432 ± 0.06
8.631 ± 0.068
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.585 ± 0.026
3.77 ± 0.044
4.51 ± 0.044
3.706 ± 0.038
5.7 ± 0.066
6.807 ± 0.061
5.964 ± 0.064
7.847 ± 0.054
1.225 ± 0.024
2.539 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here