Amino acid dipepetide frequency for Raphanus sativus (Radish) (Raphanus raphanistrum var. sativus)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.158AlaAla: 6.158 ± 0.026	1.143AlaCys: 1.143 ± 0.008	2.95AlaAsp: 2.95 ± 0.012	4.272AlaGlu: 4.272 ± 0.016	2.663AlaPhe: 2.663 ± 0.013	3.84AlaGly: 3.84 ± 0.017	1.204AlaHis: 1.204 ± 0.008	3.349AlaIle: 3.349 ± 0.013	3.973AlaLys: 3.973 ± 0.015	6.186AlaLeu: 6.186 ± 0.02	1.764AlaMet: 1.764 ± 0.008	2.393AlaAsn: 2.393 ± 0.011	2.747AlaPro: 2.747 ± 0.014	1.966AlaGln: 1.966 ± 0.01	3.392AlaArg: 3.392 ± 0.014	5.981AlaSer: 5.981 ± 0.019	3.625AlaThr: 3.625 ± 0.014	4.813AlaVal: 4.813 ± 0.019	0.748AlaTrp: 0.748 ± 0.006	1.757AlaTyr: 1.757 ± 0.01	0.002AlaXaa: 0.002 ± 0.0
Cys	0.88CysAla: 0.88 ± 0.007	0.52CysCys: 0.52 ± 0.006	0.911CysAsp: 0.911 ± 0.007	0.914CysGlu: 0.914 ± 0.007	0.927CysPhe: 0.927 ± 0.007	1.391CysGly: 1.391 ± 0.009	0.433CysHis: 0.433 ± 0.005	0.911CysIle: 0.911 ± 0.006	1.119CysLys: 1.119 ± 0.007	1.88CysLeu: 1.88 ± 0.011	0.409CysMet: 0.409 ± 0.005	0.786CysAsn: 0.786 ± 0.007	0.901CysPro: 0.901 ± 0.008	0.552CysGln: 0.552 ± 0.005	1.044CysArg: 1.044 ± 0.008	1.761CysSer: 1.761 ± 0.011	0.813CysThr: 0.813 ± 0.007	1.198CysVal: 1.198 ± 0.008	0.229CysTrp: 0.229 ± 0.003	0.559CysTyr: 0.559 ± 0.006	0.001CysXaa: 0.001 ± 0.0
Asp	3.359AspAla: 3.359 ± 0.015	0.936AspCys: 0.936 ± 0.007	4.051AspAsp: 4.051 ± 0.019	4.296AspGlu: 4.296 ± 0.017	2.298AspPhe: 2.298 ± 0.01	3.779AspGly: 3.779 ± 0.015	1.339AspHis: 1.339 ± 0.009	2.861AspIle: 2.861 ± 0.013	2.921AspLys: 2.921 ± 0.014	5.167AspLeu: 5.167 ± 0.018	1.339AspMet: 1.339 ± 0.008	2.047AspAsn: 2.047 ± 0.011	2.666AspPro: 2.666 ± 0.012	1.82AspGln: 1.82 ± 0.01	2.442AspArg: 2.442 ± 0.011	4.342AspSer: 4.342 ± 0.018	2.337AspThr: 2.337 ± 0.011	3.893AspVal: 3.893 ± 0.012	0.702AspTrp: 0.702 ± 0.006	1.577AspTyr: 1.577 ± 0.009	0.001AspXaa: 0.001 ± 0.0
Glu	5.02GluAla: 5.02 ± 0.018	0.905GluCys: 0.905 ± 0.007	4.309GluAsp: 4.309 ± 0.019	7.463GluGlu: 7.463 ± 0.039	2.402GluPhe: 2.402 ± 0.012	3.629GluGly: 3.629 ± 0.014	1.255GluHis: 1.255 ± 0.007	3.774GluIle: 3.774 ± 0.013	5.093GluLys: 5.093 ± 0.023	5.801GluLeu: 5.801 ± 0.022	1.847GluMet: 1.847 ± 0.009	2.973GluAsn: 2.973 ± 0.013	2.26GluPro: 2.26 ± 0.012	2.104GluGln: 2.104 ± 0.012	3.651GluArg: 3.651 ± 0.016	4.944GluSer: 4.944 ± 0.02	3.796GluThr: 3.796 ± 0.018	4.321GluVal: 4.321 ± 0.017	0.774GluTrp: 0.774 ± 0.006	1.664GluTyr: 1.664 ± 0.009	0.002GluXaa: 0.002 ± 0.0
Phe	2.435PheAla: 2.435 ± 0.012	0.865PheCys: 0.865 ± 0.006	2.352PheAsp: 2.352 ± 0.011	2.313PheGlu: 2.313 ± 0.012	1.957PhePhe: 1.957 ± 0.011	2.992PheGly: 2.992 ± 0.013	1.05PheHis: 1.05 ± 0.008	1.96PheIle: 1.96 ± 0.011	2.214PheLys: 2.214 ± 0.009	4.22PheLeu: 4.22 ± 0.016	0.988PheMet: 0.988 ± 0.006	1.675PheAsn: 1.675 ± 0.009	2.047PhePro: 2.047 ± 0.01	1.456PheGln: 1.456 ± 0.008	2.089PheArg: 2.089 ± 0.009	4.05PheSer: 4.05 ± 0.014	2.164PheThr: 2.164 ± 0.011	2.785PheVal: 2.785 ± 0.013	0.571PheTrp: 0.571 ± 0.005	1.236PheTyr: 1.236 ± 0.007	0.002PheXaa: 0.002 ± 0.0
Gly	3.564GlyAla: 3.564 ± 0.016	1.242GlyCys: 1.242 ± 0.01	3.586GlyAsp: 3.586 ± 0.013	3.99GlyGlu: 3.99 ± 0.015	3.171GlyPhe: 3.171 ± 0.013	6.016GlyGly: 6.016 ± 0.045	1.432GlyHis: 1.432 ± 0.01	3.25GlyIle: 3.25 ± 0.015	4.151GlyLys: 4.151 ± 0.018	5.684GlyLeu: 5.684 ± 0.021	1.469GlyMet: 1.469 ± 0.01	2.956GlyAsn: 2.956 ± 0.013	2.287GlyPro: 2.287 ± 0.012	1.958GlyGln: 1.958 ± 0.01	3.64GlyArg: 3.64 ± 0.018	5.93GlySer: 5.93 ± 0.023	3.206GlyThr: 3.206 ± 0.014	4.28GlyVal: 4.28 ± 0.015	0.857GlyTrp: 0.857 ± 0.006	2.117GlyTyr: 2.117 ± 0.013	0.002GlyXaa: 0.002 ± 0.0
His	1.246HisAla: 1.246 ± 0.008	0.493HisCys: 0.493 ± 0.005	1.117HisAsp: 1.117 ± 0.008	1.317HisGlu: 1.317 ± 0.008	0.941HisPhe: 0.941 ± 0.007	1.659HisGly: 1.659 ± 0.01	1.08HisHis: 1.08 ± 0.01	1.135HisIle: 1.135 ± 0.008	1.205HisLys: 1.205 ± 0.008	2.305HisLeu: 2.305 ± 0.012	0.572HisMet: 0.572 ± 0.005	0.947HisAsn: 0.947 ± 0.006	1.284HisPro: 1.284 ± 0.01	1.058HisGln: 1.058 ± 0.008	1.391HisArg: 1.391 ± 0.008	1.808HisSer: 1.808 ± 0.01	0.972HisThr: 0.972 ± 0.007	1.572HisVal: 1.572 ± 0.009	0.297HisTrp: 0.297 ± 0.004	0.684HisTyr: 0.684 ± 0.006	0.001HisXaa: 0.001 ± 0.0
Ile	3.296IleAla: 3.296 ± 0.014	1.041IleCys: 1.041 ± 0.007	2.808IleAsp: 2.808 ± 0.012	3.005IleGlu: 3.005 ± 0.014	2.068IlePhe: 2.068 ± 0.009	3.169IleGly: 3.169 ± 0.013	1.217IleHis: 1.217 ± 0.007	2.546IleIle: 2.546 ± 0.012	2.899IleLys: 2.899 ± 0.013	4.702IleLeu: 4.702 ± 0.015	1.098IleMet: 1.098 ± 0.007	2.088IleAsn: 2.088 ± 0.011	2.747IlePro: 2.747 ± 0.016	1.85IleGln: 1.85 ± 0.01	2.631IleArg: 2.631 ± 0.011	4.599IleSer: 4.599 ± 0.014	2.682IleThr: 2.682 ± 0.013	3.315IleVal: 3.315 ± 0.015	0.682IleTrp: 0.682 ± 0.005	1.426IleTyr: 1.426 ± 0.008	0.002IleXaa: 0.002 ± 0.0
Lys	4.103LysAla: 4.103 ± 0.018	0.976LysCys: 0.976 ± 0.007	3.337LysAsp: 3.337 ± 0.014	4.949LysGlu: 4.949 ± 0.023	2.069LysPhe: 2.069 ± 0.01	3.553LysGly: 3.553 ± 0.014	1.364LysHis: 1.364 ± 0.009	3.265LysIle: 3.265 ± 0.013	5.396LysLys: 5.396 ± 0.025	6.025LysLeu: 6.025 ± 0.021	1.564LysMet: 1.564 ± 0.01	2.647LysAsn: 2.647 ± 0.013	2.99LysPro: 2.99 ± 0.016	2.311LysGln: 2.311 ± 0.011	3.984LysArg: 3.984 ± 0.019	4.738LysSer: 4.738 ± 0.016	3.417LysThr: 3.417 ± 0.014	3.862LysVal: 3.862 ± 0.015	0.83LysTrp: 0.83 ± 0.007	1.569LysTyr: 1.569 ± 0.01	0.002LysXaa: 0.002 ± 0.0
Leu	6.199LeuAla: 6.199 ± 0.02	1.853LeuCys: 1.853 ± 0.01	4.98LeuAsp: 4.98 ± 0.017	6.209LeuGlu: 6.209 ± 0.022	3.807LeuPhe: 3.807 ± 0.013	5.543LeuGly: 5.543 ± 0.019	2.388LeuHis: 2.388 ± 0.011	4.372LeuIle: 4.372 ± 0.018	6.1LeuLys: 6.1 ± 0.02	9.548LeuLeu: 9.548 ± 0.03	2.188LeuMet: 2.188 ± 0.009	3.717LeuAsn: 3.717 ± 0.015	5.024LeuPro: 5.024 ± 0.017	3.858LeuGln: 3.858 ± 0.016	5.479LeuArg: 5.479 ± 0.021	8.473LeuSer: 8.473 ± 0.028	4.642LeuThr: 4.642 ± 0.015	6.481LeuVal: 6.481 ± 0.021	1.17LeuTrp: 1.17 ± 0.008	2.407LeuTyr: 2.407 ± 0.013	0.002LeuXaa: 0.002 ± 0.0
Met	1.994MetAla: 1.994 ± 0.01	0.34MetCys: 0.34 ± 0.004	1.429MetAsp: 1.429 ± 0.009	2.004MetGlu: 2.004 ± 0.009	0.941MetPhe: 0.941 ± 0.007	1.526MetGly: 1.526 ± 0.01	0.479MetHis: 0.479 ± 0.005	1.268MetIle: 1.268 ± 0.008	1.667MetLys: 1.667 ± 0.01	2.078MetLeu: 2.078 ± 0.01	0.806MetMet: 0.806 ± 0.007	1.06MetAsn: 1.06 ± 0.008	0.946MetPro: 0.946 ± 0.008	0.817MetGln: 0.817 ± 0.007	1.299MetArg: 1.299 ± 0.008	1.98MetSer: 1.98 ± 0.01	1.132MetThr: 1.132 ± 0.008	1.73MetVal: 1.73 ± 0.009	0.276MetTrp: 0.276 ± 0.004	0.619MetTyr: 0.619 ± 0.006	0.001MetXaa: 0.001 ± 0.0
Asn	2.538AsnAla: 2.538 ± 0.013	0.747AsnCys: 0.747 ± 0.006	2.007AsnAsp: 2.007 ± 0.011	2.429AsnGlu: 2.429 ± 0.012	1.636AsnPhe: 1.636 ± 0.01	3.129AsnGly: 3.129 ± 0.014	1.138AsnHis: 1.138 ± 0.008	2.225AsnIle: 2.225 ± 0.01	2.482AsnLys: 2.482 ± 0.012	4.265AsnLeu: 4.265 ± 0.02	1.097AsnMet: 1.097 ± 0.008	2.512AsnAsn: 2.512 ± 0.018	2.356AsnPro: 2.356 ± 0.013	1.707AsnGln: 1.707 ± 0.011	2.176AsnArg: 2.176 ± 0.012	3.502AsnSer: 3.502 ± 0.014	2.072AsnThr: 2.072 ± 0.009	2.827AsnVal: 2.827 ± 0.011	0.536AsnTrp: 0.536 ± 0.005	1.191AsnTyr: 1.191 ± 0.008	0.001AsnXaa: 0.001 ± 0.0
Pro	2.885ProAla: 2.885 ± 0.013	0.755ProCys: 0.755 ± 0.006	2.473ProAsp: 2.473 ± 0.012	3.366ProGlu: 3.366 ± 0.014	1.915ProPhe: 1.915 ± 0.011	2.687ProGly: 2.687 ± 0.014	1.06ProHis: 1.06 ± 0.008	2.098ProIle: 2.098 ± 0.008	2.831ProLys: 2.831 ± 0.013	4.253ProLeu: 4.253 ± 0.016	0.975ProMet: 0.975 ± 0.007	2.121ProAsn: 2.121 ± 0.011	4.347ProPro: 4.347 ± 0.041	1.817ProGln: 1.817 ± 0.015	2.626ProArg: 2.626 ± 0.013	5.176ProSer: 5.176 ± 0.022	2.67ProThr: 2.67 ± 0.014	3.311ProVal: 3.311 ± 0.014	0.597ProTrp: 0.597 ± 0.006	1.335ProTyr: 1.335 ± 0.01	0.002ProXaa: 0.002 ± 0.0
Gln	2.312GlnAla: 2.312 ± 0.012	0.538GlnCys: 0.538 ± 0.005	1.688GlnAsp: 1.688 ± 0.009	2.551GlnGlu: 2.551 ± 0.013	1.276GlnPhe: 1.276 ± 0.008	2.117GlnGly: 2.117 ± 0.01	0.857GlnHis: 0.857 ± 0.008	1.823GlnIle: 1.823 ± 0.01	2.184GlnLys: 2.184 ± 0.012	3.191GlnLeu: 3.191 ± 0.015	0.918GlnMet: 0.918 ± 0.008	1.586GlnAsn: 1.586 ± 0.01	1.716GlnPro: 1.716 ± 0.012	2.128GlnGln: 2.128 ± 0.028	2.211GlnArg: 2.211 ± 0.012	2.787GlnSer: 2.787 ± 0.013	1.881GlnThr: 1.881 ± 0.01	2.316GlnVal: 2.316 ± 0.012	0.439GlnTrp: 0.439 ± 0.005	0.873GlnTyr: 0.873 ± 0.007	0.001GlnXaa: 0.001 ± 0.0
Arg	3.165ArgAla: 3.165 ± 0.013	1.056ArgCys: 1.056 ± 0.007	2.879ArgAsp: 2.879 ± 0.013	3.605ArgGlu: 3.605 ± 0.015	2.485ArgPhe: 2.485 ± 0.011	3.472ArgGly: 3.472 ± 0.017	1.256ArgHis: 1.256 ± 0.009	2.821ArgIle: 2.821 ± 0.012	3.897ArgLys: 3.897 ± 0.017	5.226ArgLeu: 5.226 ± 0.016	1.327ArgMet: 1.327 ± 0.008	2.488ArgAsn: 2.488 ± 0.012	2.397ArgPro: 2.397 ± 0.012	1.871ArgGln: 1.871 ± 0.011	4.349ArgArg: 4.349 ± 0.021	4.789ArgSer: 4.789 ± 0.019	2.578ArgThr: 2.578 ± 0.012	3.629ArgVal: 3.629 ± 0.015	0.765ArgTrp: 0.765 ± 0.006	1.536ArgTyr: 1.536 ± 0.009	0.002ArgXaa: 0.002 ± 0.0
Ser	5.154SerAla: 5.154 ± 0.018	1.681SerCys: 1.681 ± 0.009	4.674SerAsp: 4.674 ± 0.017	5.019SerGlu: 5.019 ± 0.021	4.047SerPhe: 4.047 ± 0.015	6.034SerGly: 6.034 ± 0.024	2.024SerHis: 2.024 ± 0.01	4.072SerIle: 4.072 ± 0.014	5.015SerLys: 5.015 ± 0.018	8.919SerLeu: 8.919 ± 0.028	1.998SerMet: 1.998 ± 0.009	3.791SerAsn: 3.791 ± 0.014	4.884SerPro: 4.884 ± 0.025	3.021SerGln: 3.021 ± 0.014	4.773SerArg: 4.773 ± 0.019	12.148SerSer: 12.148 ± 0.043	4.62SerThr: 4.62 ± 0.016	5.675SerVal: 5.675 ± 0.018	1.157SerTrp: 1.157 ± 0.008	2.427SerTyr: 2.427 ± 0.012	0.003SerXaa: 0.003 ± 0.0
Thr	3.419ThrAla: 3.419 ± 0.018	1.01ThrCys: 1.01 ± 0.007	2.486ThrAsp: 2.486 ± 0.011	3.181ThrGlu: 3.181 ± 0.015	2.086ThrPhe: 2.086 ± 0.009	3.465ThrGly: 3.465 ± 0.014	1.073ThrHis: 1.073 ± 0.007	2.627ThrIle: 2.627 ± 0.012	3.019ThrLys: 3.019 ± 0.013	4.729ThrLeu: 4.729 ± 0.015	1.241ThrMet: 1.241 ± 0.007	2.155ThrAsn: 2.155 ± 0.011	2.735ThrPro: 2.735 ± 0.015	1.626ThrGln: 1.626 ± 0.01	2.727ThrArg: 2.727 ± 0.011	4.86ThrSer: 4.86 ± 0.017	3.468ThrThr: 3.468 ± 0.016	3.652ThrVal: 3.652 ± 0.016	0.705ThrTrp: 0.705 ± 0.006	1.405ThrTyr: 1.405 ± 0.008	0.002ThrXaa: 0.002 ± 0.0
Val	4.785ValAla: 4.785 ± 0.017	1.204ValCys: 1.204 ± 0.007	3.863ValAsp: 3.863 ± 0.015	4.655ValGlu: 4.655 ± 0.015	2.853ValPhe: 2.853 ± 0.012	3.955ValGly: 3.955 ± 0.016	1.456ValHis: 1.456 ± 0.009	3.371ValIle: 3.371 ± 0.014	4.251ValLys: 4.251 ± 0.017	6.359ValLeu: 6.359 ± 0.02	1.692ValMet: 1.692 ± 0.009	2.674ValAsn: 2.674 ± 0.011	3.239ValPro: 3.239 ± 0.014	2.148ValGln: 2.148 ± 0.011	3.293ValArg: 3.293 ± 0.013	5.964ValSer: 5.964 ± 0.018	3.591ValThr: 3.591 ± 0.013	5.234ValVal: 5.234 ± 0.019	0.851ValTrp: 0.851 ± 0.007	2.05ValTyr: 2.05 ± 0.012	0.002ValXaa: 0.002 ± 0.0
Trp	0.702TrpAla: 0.702 ± 0.006	0.243TrpCys: 0.243 ± 0.003	0.714TrpAsp: 0.714 ± 0.007	0.802TrpGlu: 0.802 ± 0.007	0.601TrpPhe: 0.601 ± 0.006	0.715TrpGly: 0.715 ± 0.007	0.268TrpHis: 0.268 ± 0.004	0.733TrpIle: 0.733 ± 0.006	0.944TrpLys: 0.944 ± 0.008	1.193TrpLeu: 1.193 ± 0.008	0.336TrpMet: 0.336 ± 0.003	0.692TrpAsn: 0.692 ± 0.006	0.456TrpPro: 0.456 ± 0.004	0.392TrpGln: 0.392 ± 0.004	0.933TrpArg: 0.933 ± 0.007	1.069TrpSer: 1.069 ± 0.007	0.665TrpThr: 0.665 ± 0.006	0.781TrpVal: 0.781 ± 0.006	0.24TrpTrp: 0.24 ± 0.004	0.354TrpTyr: 0.354 ± 0.004	0.001TrpXaa: 0.001 ± 0.0
Tyr	1.712TyrAla: 1.712 ± 0.01	0.589TyrCys: 0.589 ± 0.006	1.56TyrAsp: 1.56 ± 0.01	1.646TyrGlu: 1.646 ± 0.008	1.277TyrPhe: 1.277 ± 0.007	2.114TyrGly: 2.114 ± 0.011	0.711TyrHis: 0.711 ± 0.006	1.41TyrIle: 1.41 ± 0.008	1.584TyrLys: 1.584 ± 0.009	2.705TyrLeu: 2.705 ± 0.012	0.761TyrMet: 0.761 ± 0.006	1.262TyrAsn: 1.262 ± 0.008	1.271TyrPro: 1.271 ± 0.01	0.932TyrGln: 0.932 ± 0.006	1.46TyrArg: 1.46 ± 0.008	2.231TyrSer: 2.231 ± 0.011	1.339TyrThr: 1.339 ± 0.008	1.816TyrVal: 1.816 ± 0.01	0.406TyrTrp: 0.406 ± 0.005	1.016TyrTyr: 1.016 ± 0.009	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.0	0.001XaaCys: 0.001 ± 0.0	0.001XaaAsp: 0.001 ± 0.0	0.002XaaGlu: 0.002 ± 0.0	0.002XaaPhe: 0.002 ± 0.0	0.002XaaGly: 0.002 ± 0.0	0.001XaaHis: 0.001 ± 0.0	0.002XaaIle: 0.002 ± 0.0	0.003XaaLys: 0.003 ± 0.0	0.003XaaLeu: 0.003 ± 0.0	0.001XaaMet: 0.001 ± 0.0	0.001XaaAsn: 0.001 ± 0.0	0.001XaaPro: 0.001 ± 0.0	0.001XaaGln: 0.001 ± 0.0	0.003XaaArg: 0.003 ± 0.0	0.003XaaSer: 0.003 ± 0.0	0.002XaaThr: 0.002 ± 0.0	0.002XaaVal: 0.002 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.0	0.002XaaXaa: 0.002 ± 0.001

Statistics based on 52798 proteins (21790941 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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