Amino acid dipepetide frequency for Paenibacillus sp. D9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	14.308AlaAla: 14.308 ± 0.208	0.844AlaCys: 0.844 ± 0.022	5.349AlaAsp: 5.349 ± 0.066	6.783AlaGlu: 6.783 ± 0.095	3.707AlaPhe: 3.707 ± 0.06	9.854AlaGly: 9.854 ± 0.125	1.488AlaHis: 1.488 ± 0.037	4.954AlaIle: 4.954 ± 0.076	4.248AlaLys: 4.248 ± 0.062	9.593AlaLeu: 9.593 ± 0.114	2.734AlaMet: 2.734 ± 0.051	2.297AlaAsn: 2.297 ± 0.055	3.431AlaPro: 3.431 ± 0.068	2.705AlaGln: 2.705 ± 0.049	4.841AlaArg: 4.841 ± 0.063	6.851AlaSer: 6.851 ± 0.072	3.467AlaThr: 3.467 ± 0.053	8.047AlaVal: 8.047 ± 0.086	1.225AlaTrp: 1.225 ± 0.032	2.9AlaTyr: 2.9 ± 0.057	0.002AlaXaa: 0.002 ± 0.001
Cys	0.571CysAla: 0.571 ± 0.02	0.107CysCys: 0.107 ± 0.01	0.344CysAsp: 0.344 ± 0.017	0.361CysGlu: 0.361 ± 0.016	0.267CysPhe: 0.267 ± 0.015	0.816CysGly: 0.816 ± 0.025	0.168CysHis: 0.168 ± 0.014	0.383CysIle: 0.383 ± 0.017	0.231CysLys: 0.231 ± 0.013	0.756CysLeu: 0.756 ± 0.028	0.206CysMet: 0.206 ± 0.013	0.195CysAsn: 0.195 ± 0.012	0.356CysPro: 0.356 ± 0.017	0.167CysGln: 0.167 ± 0.01	0.564CysArg: 0.564 ± 0.022	0.583CysSer: 0.583 ± 0.023	0.364CysThr: 0.364 ± 0.015	0.408CysVal: 0.408 ± 0.018	0.103CysTrp: 0.103 ± 0.008	0.204CysTyr: 0.204 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	4.732AspAla: 4.732 ± 0.06	0.342AspCys: 0.342 ± 0.019	2.325AspAsp: 2.325 ± 0.048	3.662AspGlu: 3.662 ± 0.058	1.967AspPhe: 1.967 ± 0.038	4.644AspGly: 4.644 ± 0.072	0.905AspHis: 0.905 ± 0.03	3.169AspIle: 3.169 ± 0.05	2.56AspLys: 2.56 ± 0.045	4.87AspLeu: 4.87 ± 0.065	1.334AspMet: 1.334 ± 0.034	1.583AspAsn: 1.583 ± 0.036	2.558AspPro: 2.558 ± 0.039	1.516AspGln: 1.516 ± 0.034	3.314AspArg: 3.314 ± 0.051	3.194AspSer: 3.194 ± 0.053	2.323AspThr: 2.323 ± 0.043	3.322AspVal: 3.322 ± 0.049	0.831AspTrp: 0.831 ± 0.026	2.077AspTyr: 2.077 ± 0.048	0.0AspXaa: 0.0 ± 0.0
Glu	7.384GluAla: 7.384 ± 0.095	0.33GluCys: 0.33 ± 0.019	3.081GluAsp: 3.081 ± 0.052	5.451GluGlu: 5.451 ± 0.079	1.967GluPhe: 1.967 ± 0.035	4.982GluGly: 4.982 ± 0.073	1.399GluHis: 1.399 ± 0.039	3.81GluIle: 3.81 ± 0.056	3.448GluLys: 3.448 ± 0.061	7.692GluLeu: 7.692 ± 0.093	1.923GluMet: 1.923 ± 0.042	1.916GluAsn: 1.916 ± 0.042	2.607GluPro: 2.607 ± 0.046	3.136GluGln: 3.136 ± 0.054	4.519GluArg: 4.519 ± 0.072	3.541GluSer: 3.541 ± 0.054	3.048GluThr: 3.048 ± 0.046	4.008GluVal: 4.008 ± 0.061	0.949GluTrp: 0.949 ± 0.029	1.613GluTyr: 1.613 ± 0.037	0.0GluXaa: 0.0 ± 0.0
Phe	3.443PheAla: 3.443 ± 0.052	0.314PheCys: 0.314 ± 0.014	2.069PheAsp: 2.069 ± 0.043	2.056PheGlu: 2.056 ± 0.037	1.711PhePhe: 1.711 ± 0.042	3.38PheGly: 3.38 ± 0.051	0.865PheHis: 0.865 ± 0.027	2.304PheIle: 2.304 ± 0.048	1.715PheLys: 1.715 ± 0.042	3.77PheLeu: 3.77 ± 0.073	1.077PheMet: 1.077 ± 0.031	1.37PheAsn: 1.37 ± 0.032	1.559PhePro: 1.559 ± 0.036	1.27PheGln: 1.27 ± 0.031	2.361PheArg: 2.361 ± 0.043	2.658PheSer: 2.658 ± 0.055	2.089PheThr: 2.089 ± 0.048	2.723PheVal: 2.723 ± 0.046	0.518PheTrp: 0.518 ± 0.021	1.307PheTyr: 1.307 ± 0.033	0.0PheXaa: 0.0 ± 0.0
Gly	7.34GlyAla: 7.34 ± 0.101	0.805GlyCys: 0.805 ± 0.027	3.861GlyAsp: 3.861 ± 0.063	4.945GlyGlu: 4.945 ± 0.074	3.411GlyPhe: 3.411 ± 0.052	7.261GlyGly: 7.261 ± 0.126	1.511GlyHis: 1.511 ± 0.039	5.749GlyIle: 5.749 ± 0.062	4.573GlyLys: 4.573 ± 0.066	8.258GlyLeu: 8.258 ± 0.097	2.791GlyMet: 2.791 ± 0.051	2.516GlyAsn: 2.516 ± 0.048	2.339GlyPro: 2.339 ± 0.043	2.723GlyGln: 2.723 ± 0.044	4.959GlyArg: 4.959 ± 0.082	6.225GlySer: 6.225 ± 0.085	4.815GlyThr: 4.815 ± 0.071	5.3GlyVal: 5.3 ± 0.064	1.315GlyTrp: 1.315 ± 0.034	2.954GlyTyr: 2.954 ± 0.047	0.003GlyXaa: 0.003 ± 0.002
His	1.806HisAla: 1.806 ± 0.036	0.174HisCys: 0.174 ± 0.011	0.975HisAsp: 0.975 ± 0.027	1.253HisGlu: 1.253 ± 0.035	0.889HisPhe: 0.889 ± 0.03	1.551HisGly: 1.551 ± 0.035	0.537HisHis: 0.537 ± 0.022	1.078HisIle: 1.078 ± 0.038	0.663HisLys: 0.663 ± 0.025	1.947HisLeu: 1.947 ± 0.048	0.497HisMet: 0.497 ± 0.019	0.512HisAsn: 0.512 ± 0.019	1.289HisPro: 1.289 ± 0.032	0.585HisGln: 0.585 ± 0.021	1.125HisArg: 1.125 ± 0.029	1.087HisSer: 1.087 ± 0.029	0.864HisThr: 0.864 ± 0.026	1.343HisVal: 1.343 ± 0.033	0.256HisTrp: 0.256 ± 0.014	0.76HisTyr: 0.76 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.93IleAla: 5.93 ± 0.08	0.467IleCys: 0.467 ± 0.02	3.153IleAsp: 3.153 ± 0.048	3.603IleGlu: 3.603 ± 0.059	1.953IlePhe: 1.953 ± 0.046	5.451IleGly: 5.451 ± 0.077	1.204IleHis: 1.204 ± 0.03	3.099IleIle: 3.099 ± 0.06	2.368IleLys: 2.368 ± 0.05	5.29IleLeu: 5.29 ± 0.084	1.421IleMet: 1.421 ± 0.034	1.817IleAsn: 1.817 ± 0.04	2.922IlePro: 2.922 ± 0.045	2.01IleGln: 2.01 ± 0.044	4.129IleArg: 4.129 ± 0.056	3.717IleSer: 3.717 ± 0.058	2.84IleThr: 2.84 ± 0.05	4.689IleVal: 4.689 ± 0.069	0.618IleTrp: 0.618 ± 0.024	1.634IleTyr: 1.634 ± 0.036	0.0IleXaa: 0.0 ± 0.0
Lys	4.466LysAla: 4.466 ± 0.069	0.179LysCys: 0.179 ± 0.012	2.647LysAsp: 2.647 ± 0.05	3.802LysGlu: 3.802 ± 0.065	1.31LysPhe: 1.31 ± 0.033	3.584LysGly: 3.584 ± 0.054	0.886LysHis: 0.886 ± 0.025	2.371LysIle: 2.371 ± 0.053	2.545LysLys: 2.545 ± 0.061	5.212LysLeu: 5.212 ± 0.061	1.307LysMet: 1.307 ± 0.037	1.542LysAsn: 1.542 ± 0.042	2.404LysPro: 2.404 ± 0.044	1.931LysGln: 1.931 ± 0.041	2.727LysArg: 2.727 ± 0.047	2.719LysSer: 2.719 ± 0.05	2.485LysThr: 2.485 ± 0.05	3.01LysVal: 3.01 ± 0.049	0.592LysTrp: 0.592 ± 0.022	1.347LysTyr: 1.347 ± 0.035	0.0LysXaa: 0.0 ± 0.0
Leu	10.408LeuAla: 10.408 ± 0.131	0.753LeuCys: 0.753 ± 0.027	5.592LeuAsp: 5.592 ± 0.064	6.883LeuGlu: 6.883 ± 0.085	4.361LeuPhe: 4.361 ± 0.069	7.511LeuGly: 7.511 ± 0.089	2.085LeuHis: 2.085 ± 0.043	5.837LeuIle: 5.837 ± 0.089	4.888LeuLys: 4.888 ± 0.062	12.241LeuLeu: 12.241 ± 0.147	2.708LeuMet: 2.708 ± 0.052	3.175LeuAsn: 3.175 ± 0.059	5.076LeuPro: 5.076 ± 0.063	4.091LeuGln: 4.091 ± 0.067	5.957LeuArg: 5.957 ± 0.077	7.156LeuSer: 7.156 ± 0.076	5.254LeuThr: 5.254 ± 0.069	6.518LeuVal: 6.518 ± 0.074	1.015LeuTrp: 1.015 ± 0.029	3.11LeuTyr: 3.11 ± 0.052	0.0LeuXaa: 0.0 ± 0.0
Met	2.749MetAla: 2.749 ± 0.051	0.123MetCys: 0.123 ± 0.01	1.594MetAsp: 1.594 ± 0.035	1.999MetGlu: 1.999 ± 0.049	0.934MetPhe: 0.934 ± 0.028	1.864MetGly: 1.864 ± 0.035	0.44MetHis: 0.44 ± 0.021	1.671MetIle: 1.671 ± 0.043	1.794MetLys: 1.794 ± 0.034	3.097MetLeu: 3.097 ± 0.056	0.82MetMet: 0.82 ± 0.025	1.289MetAsn: 1.289 ± 0.031	1.346MetPro: 1.346 ± 0.035	0.923MetGln: 0.923 ± 0.025	1.51MetArg: 1.51 ± 0.035	1.856MetSer: 1.856 ± 0.038	1.646MetThr: 1.646 ± 0.037	1.588MetVal: 1.588 ± 0.035	0.198MetTrp: 0.198 ± 0.013	0.666MetTyr: 0.666 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	2.791AsnAla: 2.791 ± 0.051	0.2AsnCys: 0.2 ± 0.014	1.49AsnAsp: 1.49 ± 0.036	1.917AsnGlu: 1.917 ± 0.042	1.037AsnPhe: 1.037 ± 0.029	2.791AsnGly: 2.791 ± 0.059	0.589AsnHis: 0.589 ± 0.022	1.836AsnIle: 1.836 ± 0.042	1.46AsnLys: 1.46 ± 0.035	2.776AsnLeu: 2.776 ± 0.053	0.86AsnMet: 0.86 ± 0.029	1.066AsnAsn: 1.066 ± 0.036	1.899AsnPro: 1.899 ± 0.041	0.983AsnGln: 0.983 ± 0.029	1.963AsnArg: 1.963 ± 0.039	1.744AsnSer: 1.744 ± 0.04	1.493AsnThr: 1.493 ± 0.044	2.153AsnVal: 2.153 ± 0.044	0.399AsnTrp: 0.399 ± 0.016	1.034AsnTyr: 1.034 ± 0.03	0.0AsnXaa: 0.0 ± 0.0
Pro	4.758ProAla: 4.758 ± 0.072	0.268ProCys: 0.268 ± 0.014	2.893ProAsp: 2.893 ± 0.051	3.695ProGlu: 3.695 ± 0.057	1.858ProPhe: 1.858 ± 0.039	3.832ProGly: 3.832 ± 0.062	0.872ProHis: 0.872 ± 0.025	2.198ProIle: 2.198 ± 0.041	1.63ProLys: 1.63 ± 0.039	4.352ProLeu: 4.352 ± 0.056	1.096ProMet: 1.096 ± 0.029	1.229ProAsn: 1.229 ± 0.031	1.531ProPro: 1.531 ± 0.034	1.362ProGln: 1.362 ± 0.033	1.765ProArg: 1.765 ± 0.039	3.0ProSer: 3.0 ± 0.055	1.866ProThr: 1.866 ± 0.053	3.455ProVal: 3.455 ± 0.047	0.566ProTrp: 0.566 ± 0.019	1.528ProTyr: 1.528 ± 0.036	0.003ProXaa: 0.003 ± 0.002
Gln	3.87GlnAla: 3.87 ± 0.061	0.185GlnCys: 0.185 ± 0.011	1.54GlnAsp: 1.54 ± 0.036	2.452GlnGlu: 2.452 ± 0.052	1.243GlnPhe: 1.243 ± 0.032	2.639GlnGly: 2.639 ± 0.053	0.628GlnHis: 0.628 ± 0.02	1.883GlnIle: 1.883 ± 0.04	1.554GlnLys: 1.554 ± 0.04	3.95GlnLeu: 3.95 ± 0.062	0.929GlnMet: 0.929 ± 0.028	1.016GlnAsn: 1.016 ± 0.029	1.639GlnPro: 1.639 ± 0.034	1.746GlnGln: 1.746 ± 0.046	2.009GlnArg: 2.009 ± 0.038	2.105GlnSer: 2.105 ± 0.041	1.69GlnThr: 1.69 ± 0.038	2.19GlnVal: 2.19 ± 0.044	0.46GlnTrp: 0.46 ± 0.021	1.028GlnTyr: 1.028 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	4.228ArgAla: 4.228 ± 0.057	0.402ArgCys: 0.402 ± 0.019	2.798ArgAsp: 2.798 ± 0.051	4.405ArgGlu: 4.405 ± 0.065	2.419ArgPhe: 2.419 ± 0.047	3.761ArgGly: 3.761 ± 0.057	1.312ArgHis: 1.312 ± 0.03	4.095ArgIle: 4.095 ± 0.055	3.123ArgLys: 3.123 ± 0.047	6.717ArgLeu: 6.717 ± 0.079	1.988ArgMet: 1.988 ± 0.042	1.835ArgAsn: 1.835 ± 0.042	2.285ArgPro: 2.285 ± 0.04	2.437ArgGln: 2.437 ± 0.047	4.027ArgArg: 4.027 ± 0.077	4.182ArgSer: 4.182 ± 0.066	3.115ArgThr: 3.115 ± 0.042	3.272ArgVal: 3.272 ± 0.053	0.774ArgTrp: 0.774 ± 0.024	2.038ArgTyr: 2.038 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	6.077SerAla: 6.077 ± 0.081	0.465SerCys: 0.465 ± 0.02	2.965SerAsp: 2.965 ± 0.05	3.696SerGlu: 3.696 ± 0.054	2.919SerPhe: 2.919 ± 0.051	6.786SerGly: 6.786 ± 0.077	1.153SerHis: 1.153 ± 0.031	3.98SerIle: 3.98 ± 0.064	2.875SerLys: 2.875 ± 0.053	6.922SerLeu: 6.922 ± 0.084	1.938SerMet: 1.938 ± 0.04	1.771SerAsn: 1.771 ± 0.046	2.835SerPro: 2.835 ± 0.054	1.932SerGln: 1.932 ± 0.043	4.2SerArg: 4.2 ± 0.053	4.624SerSer: 4.624 ± 0.072	2.811SerThr: 2.811 ± 0.051	4.707SerVal: 4.707 ± 0.082	0.888SerTrp: 0.888 ± 0.026	2.205SerTyr: 2.205 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	5.283ThrAla: 5.283 ± 0.073	0.289ThrCys: 0.289 ± 0.016	2.64ThrAsp: 2.64 ± 0.05	2.831ThrGlu: 2.831 ± 0.056	1.984ThrPhe: 1.984 ± 0.044	4.732ThrGly: 4.732 ± 0.066	0.838ThrHis: 0.838 ± 0.025	3.083ThrIle: 3.083 ± 0.054	1.899ThrLys: 1.899 ± 0.044	4.934ThrLeu: 4.934 ± 0.057	1.27ThrMet: 1.27 ± 0.032	1.42ThrAsn: 1.42 ± 0.041	2.528ThrPro: 2.528 ± 0.075	1.224ThrGln: 1.224 ± 0.032	2.251ThrArg: 2.251 ± 0.044	2.878ThrSer: 2.878 ± 0.055	2.378ThrThr: 2.378 ± 0.049	4.229ThrVal: 4.229 ± 0.073	0.539ThrTrp: 0.539 ± 0.022	1.521ThrTyr: 1.521 ± 0.037	0.001ThrXaa: 0.001 ± 0.001
Val	5.661ValAla: 5.661 ± 0.071	0.569ValCys: 0.569 ± 0.024	3.593ValAsp: 3.593 ± 0.061	4.129ValGlu: 4.129 ± 0.052	2.767ValPhe: 2.767 ± 0.05	4.584ValGly: 4.584 ± 0.069	1.434ValHis: 1.434 ± 0.033	4.233ValIle: 4.233 ± 0.071	3.437ValLys: 3.437 ± 0.053	7.577ValLeu: 7.577 ± 0.087	1.929ValMet: 1.929 ± 0.041	2.314ValAsn: 2.314 ± 0.045	3.274ValPro: 3.274 ± 0.047	2.507ValGln: 2.507 ± 0.047	4.062ValArg: 4.062 ± 0.055	4.75ValSer: 4.75 ± 0.056	3.881ValThr: 3.881 ± 0.067	4.852ValVal: 4.852 ± 0.08	0.844ValTrp: 0.844 ± 0.026	2.212ValTyr: 2.212 ± 0.047	0.001ValXaa: 0.001 ± 0.001
Trp	0.924TrpAla: 0.924 ± 0.026	0.094TrpCys: 0.094 ± 0.007	0.61TrpAsp: 0.61 ± 0.023	0.796TrpGlu: 0.796 ± 0.028	0.504TrpPhe: 0.504 ± 0.02	0.84TrpGly: 0.84 ± 0.025	0.274TrpHis: 0.274 ± 0.015	0.891TrpIle: 0.891 ± 0.028	0.676TrpLys: 0.676 ± 0.024	1.545TrpLeu: 1.545 ± 0.042	0.449TrpMet: 0.449 ± 0.017	0.586TrpAsn: 0.586 ± 0.024	0.411TrpPro: 0.411 ± 0.018	0.493TrpGln: 0.493 ± 0.017	0.691TrpArg: 0.691 ± 0.023	0.867TrpSer: 0.867 ± 0.027	0.767TrpThr: 0.767 ± 0.027	0.697TrpVal: 0.697 ± 0.023	0.17TrpTrp: 0.17 ± 0.013	0.383TrpTyr: 0.383 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.899TyrAla: 2.899 ± 0.05	0.244TyrCys: 0.244 ± 0.012	1.709TyrAsp: 1.709 ± 0.04	2.086TyrGlu: 2.086 ± 0.042	1.35TyrPhe: 1.35 ± 0.035	2.791TyrGly: 2.791 ± 0.05	0.602TyrHis: 0.602 ± 0.025	1.69TyrIle: 1.69 ± 0.032	1.38TyrLys: 1.38 ± 0.033	3.121TyrLeu: 3.121 ± 0.05	0.829TyrMet: 0.829 ± 0.03	1.031TyrAsn: 1.031 ± 0.029	1.47TyrPro: 1.47 ± 0.038	1.014TyrGln: 1.014 ± 0.028	2.235TyrArg: 2.235 ± 0.047	2.01TyrSer: 2.01 ± 0.044	1.554TyrThr: 1.554 ± 0.038	2.101TyrVal: 2.101 ± 0.037	0.405TyrTrp: 0.405 ± 0.018	1.121TyrTyr: 1.121 ± 0.034	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.004XaaGly: 0.004 ± 0.002	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.031XaaXaa: 0.031 ± 0.008

Statistics based on 4351 proteins (1342033 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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