Chlamydia trachomatis
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1102 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A655N281|A0A655N281_CHLTH NIF3-like protein OS=Chlamydia trachomatis OX=813 GN=ERS133252_00387 PE=3 SV=1
MM1 pKa = 7.8 VPDD4 pKa = 5.03 DD5 pKa = 4.77 DD6 pKa = 5.5 VGFSSCEE13 pKa = 3.97 SPPDD17 pKa = 3.61 PEE19 pKa = 5.32 LPPKK23 pKa = 10.56 LLLFGLTYY31 pKa = 10.45 FSFNTDD37 pKa = 2.95 PVACTGICSVQDD49 pKa = 3.41 CPEE52 pKa = 3.9 ASEE55 pKa = 4.15 NFLVIGSGVVGVVGVGDD72 pKa = 4.16 GVADD76 pKa = 3.7 RR77 pKa = 11.84 LYY79 pKa = 10.43 FLEE82 pKa = 4.35 KK83 pKa = 10.72 VFVIDD88 pKa = 4.1 AVVGPLVAGDD98 pKa = 4.07 DD99 pKa = 3.92 VASEE103 pKa = 4.45 VVFFGEE109 pKa = 4.77 DD110 pKa = 3.26 GGDD113 pKa = 3.33 LGVVSVFGGVTVSSRR128 pKa = 11.84 EE129 pKa = 3.83 IGSVDD134 pKa = 3.17 VGVVLFGWFQVPCMEE149 pKa = 4.59 KK150 pKa = 10.22 VRR152 pKa = 11.84 IPVFF156 pKa = 3.43
Molecular weight: 16.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.772
IPC2_protein 3.63
IPC_protein 3.605
Toseland 3.389
ProMoST 3.795
Dawson 3.605
Bjellqvist 3.77
Wikipedia 3.567
Rodwell 3.439
Grimsley 3.312
Solomon 3.592
Lehninger 3.541
Nozaki 3.732
DTASelect 3.961
Thurlkill 3.465
EMBOSS 3.567
Sillero 3.732
Patrickios 1.825
IPC_peptide 3.579
IPC2_peptide 3.706
IPC2.peptide.svr19 3.709
Protein with the highest isoelectric point:
>tr|A0A655N2I8|A0A655N2I8_CHLTH Uncharacterized protein OS=Chlamydia trachomatis OX=813 GN=ERS133252_00734 PE=4 SV=1
MM1 pKa = 6.76 VAVAAAHH8 pKa = 5.32 TTTTLAQALTGQGRR22 pKa = 11.84 SSSCKK27 pKa = 7.68 IWEE30 pKa = 4.96 VYY32 pKa = 8.64 TQMQDD37 pKa = 3.09 FLHH40 pKa = 6.43 FRR42 pKa = 11.84 THH44 pKa = 4.98 FHH46 pKa = 6.32 LGFRR50 pKa = 11.84 RR51 pKa = 11.84 LRR53 pKa = 11.84 DD54 pKa = 3.15 AKK56 pKa = 8.07 TARR59 pKa = 11.84 RR60 pKa = 11.84 RR61 pKa = 11.84 PHH63 pKa = 5.81 SFPWEE68 pKa = 3.96 KK69 pKa = 10.82 GSALVRR75 pKa = 11.84 GGAHH79 pKa = 5.52 VNFLVAHH86 pKa = 6.17 LNVVRR91 pKa = 11.84 HH92 pKa = 6.43 ADD94 pKa = 3.48 LPLEE98 pKa = 4.12 PVEE101 pKa = 4.55 RR102 pKa = 11.84 HH103 pKa = 5.69 DD104 pKa = 4.94 QIAAYY109 pKa = 9.73 GRR111 pKa = 11.84 VIARR115 pKa = 11.84 LNEE118 pKa = 3.52 IRR120 pKa = 11.84 RR121 pKa = 11.84 LSVLLLQRR129 pKa = 11.84 GAGFLHH135 pKa = 6.74 HH136 pKa = 6.69 AAEE139 pKa = 4.1 RR140 pKa = 11.84 HH141 pKa = 5.32 EE142 pKa = 4.41 PATLQDD148 pKa = 4.22 CCQKK152 pKa = 10.8 LDD154 pKa = 3.9 PLVRR158 pKa = 11.84 AAGVCFIDD166 pKa = 4.58 PLAGLHH172 pKa = 6.2 RR173 pKa = 11.84 VLAHH177 pKa = 6.94 LGHH180 pKa = 6.06 SVRR183 pKa = 11.84 TLALNRR189 pKa = 11.84 VNLDD193 pKa = 2.7 RR194 pKa = 11.84 WVVVGAKK201 pKa = 9.78 RR202 pKa = 11.84 PAPFGHH208 pKa = 6.98 PAVMVDD214 pKa = 3.1 VVGTSRR220 pKa = 11.84 VEE222 pKa = 3.74 VALTHH227 pKa = 6.19 QHH229 pKa = 6.36 DD230 pKa = 4.54 CVNVRR235 pKa = 11.84 MPLARR240 pKa = 11.84 LLVVSDD246 pKa = 4.21 FF247 pKa = 3.92
Molecular weight: 27.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.385
IPC_protein 10.35
Toseland 10.657
ProMoST 10.482
Dawson 10.716
Bjellqvist 10.511
Wikipedia 10.979
Rodwell 10.716
Grimsley 10.76
Solomon 10.921
Lehninger 10.877
Nozaki 10.687
DTASelect 10.482
Thurlkill 10.643
EMBOSS 11.082
Sillero 10.672
Patrickios 10.467
IPC_peptide 10.935
IPC2_peptide 9.911
IPC2.peptide.svr19 8.718
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1102
0
1102
229585
31
1442
208.3
23.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.449 ± 0.075
1.653 ± 0.039
4.554 ± 0.057
6.52 ± 0.09
4.803 ± 0.068
6.267 ± 0.08
2.311 ± 0.041
6.604 ± 0.071
5.878 ± 0.074
10.994 ± 0.111
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.35 ± 0.038
3.637 ± 0.062
4.299 ± 0.066
4.129 ± 0.066
4.987 ± 0.066
7.841 ± 0.087
5.065 ± 0.082
6.331 ± 0.072
1.045 ± 0.03
3.254 ± 0.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here