Thiohalospira halophila DSM 15071 
Average proteome isoelectric point is 5.78 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 2641 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|A0A1I1N820|A0A1I1N820_9GAMM Transposase (Fragment) OS=Thiohalospira halophila DSM 15071 OX=1123397 GN=SAMN05660831_00001 PE=4 SV=1 
MM1 pKa = 7.02  QRR3 pKa = 11.84  TSGFTLIEE11 pKa = 3.99  LVIVLVILGVLASVAVPQFSDD32 pKa = 3.15  LGEE35 pKa = 4.23  EE36 pKa = 4.34  AEE38 pKa = 4.49  STSVRR43 pKa = 11.84  AQANAITSANSVNVANCRR61 pKa = 11.84  LSEE64 pKa = 4.13  TDD66 pKa = 3.89  CVTGLSSCDD75 pKa = 3.36  AEE77 pKa = 4.55  KK78 pKa = 11.26  VNSLMDD84 pKa = 3.94  NFDD87 pKa = 3.38  TTTWGVADD95 pKa = 5.13  GACDD99 pKa = 4.02  DD100 pKa = 4.03  NSGGTFEE107 pKa = 4.55  ITDD110 pKa = 3.79  TSGDD114 pKa = 3.73  PYY116 pKa = 9.26  TTSTCCLTRR125 pKa = 11.84  NN126 pKa = 3.65   
 Molecular weight: 13.16 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.734 
IPC2_protein 3.757 
IPC_protein 3.694 
Toseland    3.49 
ProMoST     3.872 
Dawson      3.694 
Bjellqvist  3.859 
Wikipedia   3.643 
Rodwell     3.528 
Grimsley    3.401 
Solomon     3.668 
Lehninger   3.63 
Nozaki      3.821 
DTASelect   4.037 
Thurlkill   3.567 
EMBOSS      3.656 
Sillero     3.821 
Patrickios  0.769 
IPC_peptide 3.668 
IPC2_peptide  3.795 
IPC2.peptide.svr19  3.735 
 Protein with the highest isoelectric point: 
>tr|A0A1I1WG97|A0A1I1WG97_9GAMM 1 4-dihydroxy-2-naphthoate octaprenyltransferase OS=Thiohalospira halophila DSM 15071 OX=1123397 GN=SAMN05660831_02641 PE=4 SV=1 
MM1 pKa = 7.6  RR2 pKa = 11.84  LWPRR6 pKa = 11.84  STDD9 pKa = 3.1  KK10 pKa = 11.16  KK11 pKa = 10.61  SATLEE16 pKa = 4.46  AIFLAPEE23 pKa = 4.2  AGAPVVAVAKK33 pKa = 9.36  ATVGEE38 pKa = 4.3  AGLVGDD44 pKa = 5.3  RR45 pKa = 11.84  YY46 pKa = 10.46  AAQKK50 pKa = 10.3  GFWRR54 pKa = 11.84  GPDD57 pKa = 3.2  ACPITLIRR65 pKa = 11.84  AEE67 pKa = 4.85  DD68 pKa = 3.58  LDD70 pKa = 4.86  LIQRR74 pKa = 11.84  RR75 pKa = 11.84  HH76 pKa = 5.67  HH77 pKa = 6.13  LAVTAGEE84 pKa = 4.03  HH85 pKa = 5.8  RR86 pKa = 11.84  RR87 pKa = 11.84  NLVVRR92 pKa = 11.84  GLPSRR97 pKa = 11.84  QLTDD101 pKa = 3.34  GVLVLGDD108 pKa = 3.19  VRR110 pKa = 11.84  LRR112 pKa = 11.84  LTVPRR117 pKa = 11.84  PPCLYY122 pKa = 10.59  LEE124 pKa = 4.84  RR125 pKa = 11.84  LTQRR129 pKa = 11.84  GMGRR133 pKa = 11.84  ALRR136 pKa = 11.84  KK137 pKa = 10.13  SGGVCARR144 pKa = 11.84  IIQPGSLHH152 pKa = 7.2  AGMAIHH158 pKa = 7.1  KK159 pKa = 7.74  EE160 pKa = 3.91  HH161 pKa = 7.03  TEE163 pKa = 4.03  RR164 pKa = 11.84  EE165 pKa = 4.51  SPP167 pKa = 3.53   
 Molecular weight: 18.26 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.345 
IPC2_protein 9.399 
IPC_protein 10.072 
Toseland    10.584 
ProMoST     10.262 
Dawson      10.657 
Bjellqvist  10.379 
Wikipedia   10.877 
Rodwell     10.804 
Grimsley    10.701 
Solomon     10.804 
Lehninger   10.774 
Nozaki      10.599 
DTASelect   10.365 
Thurlkill   10.584 
EMBOSS      10.994 
Sillero     10.599 
Patrickios  10.555 
IPC_peptide 10.804 
IPC2_peptide  9.531 
IPC2.peptide.svr19  8.624 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        2641 
 
        
        0
 
        
        2641 
         
        821077
 
        39
 
        1778
 
        310.9
 
        33.93
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        12.429 ± 0.075
0.697 ± 0.017
 
        5.981 ± 0.038
7.915 ± 0.059
 
        3.065 ± 0.034
9.067 ± 0.048
 
        2.37 ± 0.026
3.908 ± 0.036
       
        2.007 ± 0.038
10.679 ± 0.067
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        2.085 ± 0.022
2.196 ± 0.026
 
        5.378 ± 0.037
3.201 ± 0.03
 
        8.462 ± 0.057
4.391 ± 0.033
 
        5.107 ± 0.028
7.393 ± 0.039
       
        1.457 ± 0.021
2.212 ± 0.026
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here