Thiohalospira halophila DSM 15071
Average proteome isoelectric point is 5.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2641 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I1N820|A0A1I1N820_9GAMM Transposase (Fragment) OS=Thiohalospira halophila DSM 15071 OX=1123397 GN=SAMN05660831_00001 PE=4 SV=1
MM1 pKa = 7.02 QRR3 pKa = 11.84 TSGFTLIEE11 pKa = 3.99 LVIVLVILGVLASVAVPQFSDD32 pKa = 3.15 LGEE35 pKa = 4.23 EE36 pKa = 4.34 AEE38 pKa = 4.49 STSVRR43 pKa = 11.84 AQANAITSANSVNVANCRR61 pKa = 11.84 LSEE64 pKa = 4.13 TDD66 pKa = 3.89 CVTGLSSCDD75 pKa = 3.36 AEE77 pKa = 4.55 KK78 pKa = 11.26 VNSLMDD84 pKa = 3.94 NFDD87 pKa = 3.38 TTTWGVADD95 pKa = 5.13 GACDD99 pKa = 4.02 DD100 pKa = 4.03 NSGGTFEE107 pKa = 4.55 ITDD110 pKa = 3.79 TSGDD114 pKa = 3.73 PYY116 pKa = 9.26 TTSTCCLTRR125 pKa = 11.84 NN126 pKa = 3.65
Molecular weight: 13.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.757
IPC_protein 3.694
Toseland 3.49
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.401
Solomon 3.668
Lehninger 3.63
Nozaki 3.821
DTASelect 4.037
Thurlkill 3.567
EMBOSS 3.656
Sillero 3.821
Patrickios 0.769
IPC_peptide 3.668
IPC2_peptide 3.795
IPC2.peptide.svr19 3.735
Protein with the highest isoelectric point:
>tr|A0A1I1WG97|A0A1I1WG97_9GAMM 1 4-dihydroxy-2-naphthoate octaprenyltransferase OS=Thiohalospira halophila DSM 15071 OX=1123397 GN=SAMN05660831_02641 PE=4 SV=1
MM1 pKa = 7.6 RR2 pKa = 11.84 LWPRR6 pKa = 11.84 STDD9 pKa = 3.1 KK10 pKa = 11.16 KK11 pKa = 10.61 SATLEE16 pKa = 4.46 AIFLAPEE23 pKa = 4.2 AGAPVVAVAKK33 pKa = 9.36 ATVGEE38 pKa = 4.3 AGLVGDD44 pKa = 5.3 RR45 pKa = 11.84 YY46 pKa = 10.46 AAQKK50 pKa = 10.3 GFWRR54 pKa = 11.84 GPDD57 pKa = 3.2 ACPITLIRR65 pKa = 11.84 AEE67 pKa = 4.85 DD68 pKa = 3.58 LDD70 pKa = 4.86 LIQRR74 pKa = 11.84 RR75 pKa = 11.84 HH76 pKa = 5.67 HH77 pKa = 6.13 LAVTAGEE84 pKa = 4.03 HH85 pKa = 5.8 RR86 pKa = 11.84 RR87 pKa = 11.84 NLVVRR92 pKa = 11.84 GLPSRR97 pKa = 11.84 QLTDD101 pKa = 3.34 GVLVLGDD108 pKa = 3.19 VRR110 pKa = 11.84 LRR112 pKa = 11.84 LTVPRR117 pKa = 11.84 PPCLYY122 pKa = 10.59 LEE124 pKa = 4.84 RR125 pKa = 11.84 LTQRR129 pKa = 11.84 GMGRR133 pKa = 11.84 ALRR136 pKa = 11.84 KK137 pKa = 10.13 SGGVCARR144 pKa = 11.84 IIQPGSLHH152 pKa = 7.2 AGMAIHH158 pKa = 7.1 KK159 pKa = 7.74 EE160 pKa = 3.91 HH161 pKa = 7.03 TEE163 pKa = 4.03 RR164 pKa = 11.84 EE165 pKa = 4.51 SPP167 pKa = 3.53
Molecular weight: 18.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.399
IPC_protein 10.072
Toseland 10.584
ProMoST 10.262
Dawson 10.657
Bjellqvist 10.379
Wikipedia 10.877
Rodwell 10.804
Grimsley 10.701
Solomon 10.804
Lehninger 10.774
Nozaki 10.599
DTASelect 10.365
Thurlkill 10.584
EMBOSS 10.994
Sillero 10.599
Patrickios 10.555
IPC_peptide 10.804
IPC2_peptide 9.531
IPC2.peptide.svr19 8.624
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2641
0
2641
821077
39
1778
310.9
33.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.429 ± 0.075
0.697 ± 0.017
5.981 ± 0.038
7.915 ± 0.059
3.065 ± 0.034
9.067 ± 0.048
2.37 ± 0.026
3.908 ± 0.036
2.007 ± 0.038
10.679 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.085 ± 0.022
2.196 ± 0.026
5.378 ± 0.037
3.201 ± 0.03
8.462 ± 0.057
4.391 ± 0.033
5.107 ± 0.028
7.393 ± 0.039
1.457 ± 0.021
2.212 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here