Thermodesulforhabdus norvegica
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2661 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I4SNQ6|A0A1I4SNQ6_9DELT Thiol:disulfide interchange protein OS=Thermodesulforhabdus norvegica OX=39841 GN=SAMN05660836_01076 PE=3 SV=1
MM1 pKa = 7.77 RR2 pKa = 11.84 KK3 pKa = 9.37 YY4 pKa = 10.99 LVMLIALVAAVAIAVPSFAVEE25 pKa = 4.36 FKK27 pKa = 10.84 YY28 pKa = 10.9 GGMYY32 pKa = 9.76 RR33 pKa = 11.84 WRR35 pKa = 11.84 IHH37 pKa = 6.64 ANEE40 pKa = 3.85 NMKK43 pKa = 10.43 DD44 pKa = 3.68 ANSDD48 pKa = 3.86 LDD50 pKa = 4.57 DD51 pKa = 3.74 TANWIDD57 pKa = 3.35 QRR59 pKa = 11.84 LRR61 pKa = 11.84 LYY63 pKa = 8.77 FTFVGSEE70 pKa = 3.75 NLQLVTKK77 pKa = 10.12 WEE79 pKa = 4.25 ADD81 pKa = 3.87 TLWGLEE87 pKa = 4.09 KK88 pKa = 10.75 GAGRR92 pKa = 11.84 HH93 pKa = 6.42 GGGDD97 pKa = 3.09 WGADD101 pKa = 3.11 AVNLEE106 pKa = 4.25 MKK108 pKa = 10.35 NVYY111 pKa = 10.32 LDD113 pKa = 3.81 FMIPNTPVRR122 pKa = 11.84 ALVGVQGLSYY132 pKa = 10.97 LDD134 pKa = 3.35 GWIVADD140 pKa = 4.05 DD141 pKa = 3.85 ASAFVLKK148 pKa = 9.52 TAFDD152 pKa = 3.91 PVKK155 pKa = 11.08 VEE157 pKa = 4.08 LGYY160 pKa = 10.43 IAGVNSDD167 pKa = 4.14 PVTDD171 pKa = 4.05 YY172 pKa = 11.85 NDD174 pKa = 3.21 MDD176 pKa = 3.96 DD177 pKa = 3.68 WFLALKK183 pKa = 9.85 YY184 pKa = 10.91 AEE186 pKa = 4.9 GPFNAAAVLFYY197 pKa = 10.69 QYY199 pKa = 11.62 AHH201 pKa = 7.22 DD202 pKa = 4.95 ADD204 pKa = 3.75 YY205 pKa = 11.0 SYY207 pKa = 10.97 PNSIGYY213 pKa = 10.01 GGTGAVDD220 pKa = 3.4 KK221 pKa = 10.68 EE222 pKa = 4.35 DD223 pKa = 3.42 NHH225 pKa = 7.98 LVDD228 pKa = 5.38 LGFSLGYY235 pKa = 10.8 KK236 pKa = 9.4 MDD238 pKa = 3.36 WLGAKK243 pKa = 10.08 VNFVKK248 pKa = 10.88 NLGGYY253 pKa = 9.99 DD254 pKa = 3.14 IAGTDD259 pKa = 3.25 EE260 pKa = 4.91 DD261 pKa = 4.26 EE262 pKa = 5.96 DD263 pKa = 4.62 YY264 pKa = 11.16 EE265 pKa = 4.55 GWMVEE270 pKa = 3.95 AALDD274 pKa = 4.11 FYY276 pKa = 10.96 MGAFTFTLGGFWTSDD291 pKa = 3.15 DD292 pKa = 3.52 FAYY295 pKa = 9.77 PAGRR299 pKa = 11.84 SHH301 pKa = 6.42 YY302 pKa = 8.2 WAEE305 pKa = 3.83 IAGLGTLDD313 pKa = 3.92 VNVAGNDD320 pKa = 3.59 WYY322 pKa = 10.02 TSSYY326 pKa = 10.73 GMGGGLPNRR335 pKa = 11.84 GDD337 pKa = 3.83 YY338 pKa = 10.93 DD339 pKa = 4.24 AGDD342 pKa = 3.75 APRR345 pKa = 11.84 NLWTIHH351 pKa = 6.99 AGVAWQALDD360 pKa = 3.52 TTKK363 pKa = 9.61 VTFNYY368 pKa = 10.28 YY369 pKa = 10.83 YY370 pKa = 10.74 LGTDD374 pKa = 3.7 DD375 pKa = 6.14 DD376 pKa = 4.41 VLANEE381 pKa = 4.71 ATDD384 pKa = 4.52 EE385 pKa = 4.19 YY386 pKa = 10.86 DD387 pKa = 4.05 DD388 pKa = 5.42 SIGHH392 pKa = 6.23 EE393 pKa = 4.13 LDD395 pKa = 4.5 LYY397 pKa = 10.56 IDD399 pKa = 4.06 HH400 pKa = 7.36 KK401 pKa = 11.69 LMDD404 pKa = 4.52 GLTLRR409 pKa = 11.84 LVGAYY414 pKa = 10.55 LIGDD418 pKa = 4.11 DD419 pKa = 4.18 ALSTNEE425 pKa = 4.17 EE426 pKa = 4.04 DD427 pKa = 4.15 DD428 pKa = 4.27 NIYY431 pKa = 10.84 EE432 pKa = 4.08 VGARR436 pKa = 11.84 LLWKK440 pKa = 10.18 FF441 pKa = 3.6
Molecular weight: 48.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.78
IPC2_protein 4.012
IPC_protein 4.05
Toseland 3.821
ProMoST 4.202
Dawson 4.037
Bjellqvist 4.19
Wikipedia 3.973
Rodwell 3.859
Grimsley 3.719
Solomon 4.037
Lehninger 3.999
Nozaki 4.139
DTASelect 4.418
Thurlkill 3.872
EMBOSS 3.986
Sillero 4.164
Patrickios 1.507
IPC_peptide 4.037
IPC2_peptide 4.139
IPC2.peptide.svr19 4.034
Protein with the highest isoelectric point:
>tr|A0A1I4T512|A0A1I4T512_9DELT Pyruvate formate lyase activating enzyme OS=Thermodesulforhabdus norvegica OX=39841 GN=SAMN05660836_01234 PE=4 SV=1
MM1 pKa = 7.62 AVPKK5 pKa = 10.38 RR6 pKa = 11.84 RR7 pKa = 11.84 TSRR10 pKa = 11.84 SRR12 pKa = 11.84 RR13 pKa = 11.84 NNRR16 pKa = 11.84 RR17 pKa = 11.84 SHH19 pKa = 6.59 DD20 pKa = 4.73 AISAPALYY28 pKa = 10.3 LCPRR32 pKa = 11.84 CKK34 pKa = 10.29 SPKK37 pKa = 9.66 LPHH40 pKa = 6.4 RR41 pKa = 11.84 VCPNCGTYY49 pKa = 10.04 RR50 pKa = 11.84 GRR52 pKa = 11.84 DD53 pKa = 3.28 VLKK56 pKa = 10.61 IEE58 pKa = 4.42 EE59 pKa = 4.2 EE60 pKa = 4.13
Molecular weight: 6.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.633
IPC_protein 10.526
Toseland 10.716
ProMoST 10.467
Dawson 10.804
Bjellqvist 10.57
Wikipedia 11.038
Rodwell 10.935
Grimsley 10.847
Solomon 10.965
Lehninger 10.935
Nozaki 10.745
DTASelect 10.54
Thurlkill 10.716
EMBOSS 11.125
Sillero 10.76
Patrickios 10.716
IPC_peptide 10.965
IPC2_peptide 9.955
IPC2.peptide.svr19 8.517
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2661
0
2661
865482
39
1866
325.2
36.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.719 ± 0.042
1.385 ± 0.021
5.031 ± 0.035
7.526 ± 0.049
4.409 ± 0.032
7.641 ± 0.041
1.979 ± 0.018
6.693 ± 0.042
5.361 ± 0.041
10.2 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.409 ± 0.02
2.94 ± 0.022
4.742 ± 0.027
2.543 ± 0.027
6.958 ± 0.041
5.577 ± 0.035
4.507 ± 0.027
7.991 ± 0.041
1.224 ± 0.02
3.166 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here