Beihai narna-like virus 15
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KHW5|A0A1L3KHW5_9VIRU RNA-dependent RNA polymerase OS=Beihai narna-like virus 15 OX=1922442 PE=4 SV=1
MM1 pKa = 7.22 QSKK4 pKa = 10.28 ALAATVPEE12 pKa = 4.27 SFILEE17 pKa = 4.22 SLLKK21 pKa = 10.57 HH22 pKa = 6.41 CEE24 pKa = 4.06 TVSKK28 pKa = 9.39 VTQTSKK34 pKa = 11.08 EE35 pKa = 3.8 LLEE38 pKa = 4.25 EE39 pKa = 4.11 FKK41 pKa = 10.67 EE42 pKa = 4.14 YY43 pKa = 10.41 CRR45 pKa = 11.84 PIMRR49 pKa = 11.84 DD50 pKa = 2.7 AAQRR54 pKa = 11.84 LSSTYY59 pKa = 10.59 SGIPSNHH66 pKa = 6.33 ASYY69 pKa = 8.02 EE70 pKa = 4.14 TTRR73 pKa = 11.84 EE74 pKa = 3.79 KK75 pKa = 11.22 GGVGRR80 pKa = 11.84 NYY82 pKa = 10.54 KK83 pKa = 10.3 LKK85 pKa = 10.71 RR86 pKa = 11.84 NLFFQPEE93 pKa = 4.2 NPRR96 pKa = 11.84 LDD98 pKa = 5.09 PITTLISGKK107 pKa = 10.52 AGIGKK112 pKa = 9.36 SLLQAKK118 pKa = 9.26 IVSKK122 pKa = 10.16 MADD125 pKa = 3.17 VLGRR129 pKa = 11.84 SPFQTSYY136 pKa = 11.09 NRR138 pKa = 11.84 NSDD141 pKa = 3.59 TEE143 pKa = 3.83 HH144 pKa = 6.43 WDD146 pKa = 3.69 GYY148 pKa = 7.83 NQQPIVMIDD157 pKa = 3.54 DD158 pKa = 4.59 FQQLRR163 pKa = 11.84 TRR165 pKa = 11.84 NYY167 pKa = 10.7 DD168 pKa = 3.23 VQQEE172 pKa = 4.19 EE173 pKa = 5.12 KK174 pKa = 11.09 EE175 pKa = 4.4 FITLNSTVDD184 pKa = 3.12 KK185 pKa = 9.68 VLPMAHH191 pKa = 6.79 LKK193 pKa = 10.79 DD194 pKa = 3.44 KK195 pKa = 10.82 GMKK198 pKa = 9.67 FNSPFIIYY206 pKa = 10.21 SSNHH210 pKa = 4.65 TLGEE214 pKa = 4.54 TICEE218 pKa = 4.16 LQRR221 pKa = 11.84 NFHH224 pKa = 5.83 SFRR227 pKa = 11.84 AIEE230 pKa = 3.79 RR231 pKa = 11.84 RR232 pKa = 11.84 FDD234 pKa = 3.45 YY235 pKa = 11.26 LIDD238 pKa = 4.12 YY239 pKa = 8.91 DD240 pKa = 4.34 PKK242 pKa = 11.22 GIGYY246 pKa = 9.37 WMVKK250 pKa = 9.94 KK251 pKa = 10.05 VVPYY255 pKa = 10.22 QYY257 pKa = 10.83 SDD259 pKa = 3.24 SQRR262 pKa = 11.84 QTNPFMVKK270 pKa = 9.37 MEE272 pKa = 3.95 QMPLTPNLDD281 pKa = 2.96 RR282 pKa = 11.84 HH283 pKa = 5.79 FQRR286 pKa = 11.84 QTIFGSSSIDD296 pKa = 3.48 EE297 pKa = 3.99 IASFFTHH304 pKa = 6.93 LMIKK308 pKa = 8.78 EE309 pKa = 4.21 WEE311 pKa = 4.27 VKK313 pKa = 10.15 IIFYY317 pKa = 10.62 QNEE320 pKa = 3.71 IRR322 pKa = 11.84 NLNLVPIGPGIHH334 pKa = 6.54 GEE336 pKa = 4.02 WISNEE341 pKa = 3.43 KK342 pKa = 9.3 HH343 pKa = 6.28 NKK345 pKa = 9.25 VKK347 pKa = 10.59 VKK349 pKa = 10.46 PILEE353 pKa = 4.14 PLKK356 pKa = 10.92 VRR358 pKa = 11.84 TITIGSSQNFMLKK371 pKa = 10.02 PISKK375 pKa = 10.51 AIFQSLQKK383 pKa = 9.92 YY384 pKa = 10.18 PEE386 pKa = 4.24 MKK388 pKa = 9.95 PCFTPEE394 pKa = 4.16 YY395 pKa = 8.39 IDD397 pKa = 3.38 SVEE400 pKa = 4.12 RR401 pKa = 11.84 LKK403 pKa = 11.03 ALPGLWLSGDD413 pKa = 3.7 YY414 pKa = 11.06 SSATDD419 pKa = 4.12 GLHH422 pKa = 6.89 SDD424 pKa = 4.47 LFRR427 pKa = 11.84 AGLSTLEE434 pKa = 4.36 EE435 pKa = 4.27 YY436 pKa = 10.74 LPCEE440 pKa = 3.44 LWKK443 pKa = 10.55 LVKK446 pKa = 10.27 RR447 pKa = 11.84 EE448 pKa = 4.14 MDD450 pKa = 3.33 PHH452 pKa = 7.47 LCEE455 pKa = 4.06 YY456 pKa = 10.48 PEE458 pKa = 4.36 GLGIPDD464 pKa = 3.79 VFQTNGQLMGSLLSFPLLSLANAFTLSKK492 pKa = 9.95 TLDD495 pKa = 3.65 RR496 pKa = 11.84 PLGDD500 pKa = 4.54 LPCLIHH506 pKa = 7.96 GDD508 pKa = 3.75 DD509 pKa = 4.5 LLAKK513 pKa = 10.42 VSLDD517 pKa = 4.32 QYY519 pKa = 11.1 LDD521 pKa = 3.15 WKK523 pKa = 10.66 AFCPKK528 pKa = 9.88 IGLSLSVGKK537 pKa = 10.41 NYY539 pKa = 9.78 ISEE542 pKa = 4.1 HH543 pKa = 5.85 WGSIDD548 pKa = 3.32 SQVYY552 pKa = 9.46 FEE554 pKa = 4.52 SEE556 pKa = 3.78 KK557 pKa = 11.15 LGTGKK562 pKa = 10.33 FSALFSKK569 pKa = 10.64 EE570 pKa = 3.84 ITCLPTLVRR579 pKa = 11.84 RR580 pKa = 11.84 GVPKK584 pKa = 10.2 PLIVSLLKK592 pKa = 10.63 KK593 pKa = 10.08 EE594 pKa = 4.23 IEE596 pKa = 4.07 RR597 pKa = 11.84 TPRR600 pKa = 11.84 SLDD603 pKa = 3.14 VSQEE607 pKa = 3.79 FGGLGIHH614 pKa = 6.63 GEE616 pKa = 4.37 PYY618 pKa = 10.27 DD619 pKa = 3.75 SRR621 pKa = 11.84 SRR623 pKa = 11.84 AVFHH627 pKa = 6.93 NMVLKK632 pKa = 10.88 SFGKK636 pKa = 9.26 RR637 pKa = 11.84 EE638 pKa = 3.69 VWGGYY643 pKa = 9.27 IYY645 pKa = 10.31 TISEE649 pKa = 4.33 SLKK652 pKa = 10.89 DD653 pKa = 3.84 MVPQRR658 pKa = 11.84 YY659 pKa = 8.59 QSPIEE664 pKa = 4.23 IPDD667 pKa = 3.84 DD668 pKa = 3.9 LEE670 pKa = 4.39 VYY672 pKa = 10.05 KK673 pKa = 10.97 KK674 pKa = 10.94 NEE676 pKa = 3.32 FRR678 pKa = 11.84 EE679 pKa = 4.15 IKK681 pKa = 10.32 RR682 pKa = 11.84 FEE684 pKa = 4.27 NLTFSQNYY692 pKa = 7.67 TPITKK697 pKa = 9.53 WSTFSEE703 pKa = 4.58 SEE705 pKa = 4.12 NPFLEE710 pKa = 4.08 NLISQSRR717 pKa = 11.84 NNYY720 pKa = 9.97 SEE722 pKa = 4.22 VLTTFDD728 pKa = 3.57 FF729 pKa = 4.72
Molecular weight: 83.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.545
IPC2_protein 6.678
IPC_protein 6.737
Toseland 6.795
ProMoST 7.146
Dawson 7.249
Bjellqvist 7.249
Wikipedia 7.22
Rodwell 7.249
Grimsley 6.927
Solomon 7.278
Lehninger 7.293
Nozaki 7.527
DTASelect 7.541
Thurlkill 7.571
EMBOSS 7.6
Sillero 7.673
Patrickios 4.329
IPC_peptide 7.293
IPC2_peptide 7.0
IPC2.peptide.svr19 6.97
Protein with the highest isoelectric point:
>tr|A0A1L3KHW5|A0A1L3KHW5_9VIRU RNA-dependent RNA polymerase OS=Beihai narna-like virus 15 OX=1922442 PE=4 SV=1
MM1 pKa = 7.22 QSKK4 pKa = 10.28 ALAATVPEE12 pKa = 4.27 SFILEE17 pKa = 4.22 SLLKK21 pKa = 10.57 HH22 pKa = 6.41 CEE24 pKa = 4.06 TVSKK28 pKa = 9.39 VTQTSKK34 pKa = 11.08 EE35 pKa = 3.8 LLEE38 pKa = 4.25 EE39 pKa = 4.11 FKK41 pKa = 10.67 EE42 pKa = 4.14 YY43 pKa = 10.41 CRR45 pKa = 11.84 PIMRR49 pKa = 11.84 DD50 pKa = 2.7 AAQRR54 pKa = 11.84 LSSTYY59 pKa = 10.59 SGIPSNHH66 pKa = 6.33 ASYY69 pKa = 8.02 EE70 pKa = 4.14 TTRR73 pKa = 11.84 EE74 pKa = 3.79 KK75 pKa = 11.22 GGVGRR80 pKa = 11.84 NYY82 pKa = 10.54 KK83 pKa = 10.3 LKK85 pKa = 10.71 RR86 pKa = 11.84 NLFFQPEE93 pKa = 4.2 NPRR96 pKa = 11.84 LDD98 pKa = 5.09 PITTLISGKK107 pKa = 10.52 AGIGKK112 pKa = 9.36 SLLQAKK118 pKa = 9.26 IVSKK122 pKa = 10.16 MADD125 pKa = 3.17 VLGRR129 pKa = 11.84 SPFQTSYY136 pKa = 11.09 NRR138 pKa = 11.84 NSDD141 pKa = 3.59 TEE143 pKa = 3.83 HH144 pKa = 6.43 WDD146 pKa = 3.69 GYY148 pKa = 7.83 NQQPIVMIDD157 pKa = 3.54 DD158 pKa = 4.59 FQQLRR163 pKa = 11.84 TRR165 pKa = 11.84 NYY167 pKa = 10.7 DD168 pKa = 3.23 VQQEE172 pKa = 4.19 EE173 pKa = 5.12 KK174 pKa = 11.09 EE175 pKa = 4.4 FITLNSTVDD184 pKa = 3.12 KK185 pKa = 9.68 VLPMAHH191 pKa = 6.79 LKK193 pKa = 10.79 DD194 pKa = 3.44 KK195 pKa = 10.82 GMKK198 pKa = 9.67 FNSPFIIYY206 pKa = 10.21 SSNHH210 pKa = 4.65 TLGEE214 pKa = 4.54 TICEE218 pKa = 4.16 LQRR221 pKa = 11.84 NFHH224 pKa = 5.83 SFRR227 pKa = 11.84 AIEE230 pKa = 3.79 RR231 pKa = 11.84 RR232 pKa = 11.84 FDD234 pKa = 3.45 YY235 pKa = 11.26 LIDD238 pKa = 4.12 YY239 pKa = 8.91 DD240 pKa = 4.34 PKK242 pKa = 11.22 GIGYY246 pKa = 9.37 WMVKK250 pKa = 9.94 KK251 pKa = 10.05 VVPYY255 pKa = 10.22 QYY257 pKa = 10.83 SDD259 pKa = 3.24 SQRR262 pKa = 11.84 QTNPFMVKK270 pKa = 9.37 MEE272 pKa = 3.95 QMPLTPNLDD281 pKa = 2.96 RR282 pKa = 11.84 HH283 pKa = 5.79 FQRR286 pKa = 11.84 QTIFGSSSIDD296 pKa = 3.48 EE297 pKa = 3.99 IASFFTHH304 pKa = 6.93 LMIKK308 pKa = 8.78 EE309 pKa = 4.21 WEE311 pKa = 4.27 VKK313 pKa = 10.15 IIFYY317 pKa = 10.62 QNEE320 pKa = 3.71 IRR322 pKa = 11.84 NLNLVPIGPGIHH334 pKa = 6.54 GEE336 pKa = 4.02 WISNEE341 pKa = 3.43 KK342 pKa = 9.3 HH343 pKa = 6.28 NKK345 pKa = 9.25 VKK347 pKa = 10.59 VKK349 pKa = 10.46 PILEE353 pKa = 4.14 PLKK356 pKa = 10.92 VRR358 pKa = 11.84 TITIGSSQNFMLKK371 pKa = 10.02 PISKK375 pKa = 10.51 AIFQSLQKK383 pKa = 9.92 YY384 pKa = 10.18 PEE386 pKa = 4.24 MKK388 pKa = 9.95 PCFTPEE394 pKa = 4.16 YY395 pKa = 8.39 IDD397 pKa = 3.38 SVEE400 pKa = 4.12 RR401 pKa = 11.84 LKK403 pKa = 11.03 ALPGLWLSGDD413 pKa = 3.7 YY414 pKa = 11.06 SSATDD419 pKa = 4.12 GLHH422 pKa = 6.89 SDD424 pKa = 4.47 LFRR427 pKa = 11.84 AGLSTLEE434 pKa = 4.36 EE435 pKa = 4.27 YY436 pKa = 10.74 LPCEE440 pKa = 3.44 LWKK443 pKa = 10.55 LVKK446 pKa = 10.27 RR447 pKa = 11.84 EE448 pKa = 4.14 MDD450 pKa = 3.33 PHH452 pKa = 7.47 LCEE455 pKa = 4.06 YY456 pKa = 10.48 PEE458 pKa = 4.36 GLGIPDD464 pKa = 3.79 VFQTNGQLMGSLLSFPLLSLANAFTLSKK492 pKa = 9.95 TLDD495 pKa = 3.65 RR496 pKa = 11.84 PLGDD500 pKa = 4.54 LPCLIHH506 pKa = 7.96 GDD508 pKa = 3.75 DD509 pKa = 4.5 LLAKK513 pKa = 10.42 VSLDD517 pKa = 4.32 QYY519 pKa = 11.1 LDD521 pKa = 3.15 WKK523 pKa = 10.66 AFCPKK528 pKa = 9.88 IGLSLSVGKK537 pKa = 10.41 NYY539 pKa = 9.78 ISEE542 pKa = 4.1 HH543 pKa = 5.85 WGSIDD548 pKa = 3.32 SQVYY552 pKa = 9.46 FEE554 pKa = 4.52 SEE556 pKa = 3.78 KK557 pKa = 11.15 LGTGKK562 pKa = 10.33 FSALFSKK569 pKa = 10.64 EE570 pKa = 3.84 ITCLPTLVRR579 pKa = 11.84 RR580 pKa = 11.84 GVPKK584 pKa = 10.2 PLIVSLLKK592 pKa = 10.63 KK593 pKa = 10.08 EE594 pKa = 4.23 IEE596 pKa = 4.07 RR597 pKa = 11.84 TPRR600 pKa = 11.84 SLDD603 pKa = 3.14 VSQEE607 pKa = 3.79 FGGLGIHH614 pKa = 6.63 GEE616 pKa = 4.37 PYY618 pKa = 10.27 DD619 pKa = 3.75 SRR621 pKa = 11.84 SRR623 pKa = 11.84 AVFHH627 pKa = 6.93 NMVLKK632 pKa = 10.88 SFGKK636 pKa = 9.26 RR637 pKa = 11.84 EE638 pKa = 3.69 VWGGYY643 pKa = 9.27 IYY645 pKa = 10.31 TISEE649 pKa = 4.33 SLKK652 pKa = 10.89 DD653 pKa = 3.84 MVPQRR658 pKa = 11.84 YY659 pKa = 8.59 QSPIEE664 pKa = 4.23 IPDD667 pKa = 3.84 DD668 pKa = 3.9 LEE670 pKa = 4.39 VYY672 pKa = 10.05 KK673 pKa = 10.97 KK674 pKa = 10.94 NEE676 pKa = 3.32 FRR678 pKa = 11.84 EE679 pKa = 4.15 IKK681 pKa = 10.32 RR682 pKa = 11.84 FEE684 pKa = 4.27 NLTFSQNYY692 pKa = 7.67 TPITKK697 pKa = 9.53 WSTFSEE703 pKa = 4.58 SEE705 pKa = 4.12 NPFLEE710 pKa = 4.08 NLISQSRR717 pKa = 11.84 NNYY720 pKa = 9.97 SEE722 pKa = 4.22 VLTTFDD728 pKa = 3.57 FF729 pKa = 4.72
Molecular weight: 83.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.545
IPC2_protein 6.678
IPC_protein 6.737
Toseland 6.795
ProMoST 7.146
Dawson 7.249
Bjellqvist 7.249
Wikipedia 7.22
Rodwell 7.249
Grimsley 6.927
Solomon 7.278
Lehninger 7.293
Nozaki 7.527
DTASelect 7.541
Thurlkill 7.571
EMBOSS 7.6
Sillero 7.673
Patrickios 4.329
IPC_peptide 7.293
IPC2_peptide 7.0
IPC2.peptide.svr19 6.97
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
729
729
729
729.0
83.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.018 ± 0.0
1.235 ± 0.0
4.801 ± 0.0
7.27 ± 0.0
5.35 ± 0.0
5.624 ± 0.0
2.195 ± 0.0
6.584 ± 0.0
7.27 ± 0.0
10.425 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.332 ± 0.0
4.252 ± 0.0
5.761 ± 0.0
4.39 ± 0.0
4.801 ± 0.0
9.191 ± 0.0
5.35 ± 0.0
4.801 ± 0.0
1.372 ± 0.0
3.978 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here