Beihai shrimp virus 4

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEC0|A0A1L3KEC0_9VIRU RNA-directed RNA polymerase OS=Beihai shrimp virus 4 OX=1922670 PE=4 SV=1
MM1 pKa = 6.8QRR3 pKa = 11.84SHH5 pKa = 6.8AKK7 pKa = 10.07LGARR11 pKa = 11.84VARR14 pKa = 11.84ATGNWTEE21 pKa = 4.42RR22 pKa = 11.84YY23 pKa = 9.95LDD25 pKa = 4.89FITPNLPTTSGGTEE39 pKa = 3.71RR40 pKa = 11.84SQWSPGQEE48 pKa = 4.02HH49 pKa = 6.57QDD51 pKa = 3.07QHH53 pKa = 6.55ARR55 pKa = 11.84PEE57 pKa = 4.18LQSQQEE63 pKa = 4.09QTVPVCDD70 pKa = 3.69VQNPTGRR77 pKa = 11.84GQSAQAQMPQDD88 pKa = 3.84WDD90 pKa = 3.43RR91 pKa = 11.84TIYY94 pKa = 10.41DD95 pKa = 3.29VRR97 pKa = 11.84TNNLTDD103 pKa = 3.18VDD105 pKa = 3.97IDD107 pKa = 3.66GGTAMKK113 pKa = 9.86TKK115 pKa = 10.29LRR117 pKa = 11.84SGLDD121 pKa = 3.14WKK123 pKa = 10.94IEE125 pKa = 3.88DD126 pKa = 4.16LGIEE130 pKa = 4.42GFRR133 pKa = 11.84ALMKK137 pKa = 10.44KK138 pKa = 9.82HH139 pKa = 6.14VDD141 pKa = 3.2PTTSPGHH148 pKa = 5.57PWKK151 pKa = 10.64EE152 pKa = 3.65LADD155 pKa = 3.84TNGQLFGYY163 pKa = 10.17DD164 pKa = 3.85PILGFVGEE172 pKa = 4.33QVEE175 pKa = 4.47TLYY178 pKa = 10.96EE179 pKa = 3.9AVKK182 pKa = 10.33IRR184 pKa = 11.84LRR186 pKa = 11.84EE187 pKa = 3.85LAEE190 pKa = 4.26KK191 pKa = 10.6PFIDD195 pKa = 6.23DD196 pKa = 3.0INLFIKK202 pKa = 10.51DD203 pKa = 4.08EE204 pKa = 3.92PHH206 pKa = 6.67KK207 pKa = 10.92AKK209 pKa = 10.66KK210 pKa = 10.31LEE212 pKa = 4.0NEE214 pKa = 4.11AYY216 pKa = 10.34RR217 pKa = 11.84LISGVGITDD226 pKa = 3.46QMVSTIMFEE235 pKa = 4.1EE236 pKa = 4.37LLEE239 pKa = 4.12EE240 pKa = 4.72VISKK244 pKa = 9.96PGEE247 pKa = 3.89YY248 pKa = 9.19STMIGWGIHH257 pKa = 5.41CEE259 pKa = 3.87EE260 pKa = 5.28GLLSMNRR267 pKa = 11.84AVRR270 pKa = 11.84SRR272 pKa = 11.84FGQNLASADD281 pKa = 3.56KK282 pKa = 11.22SSWDD286 pKa = 3.06WTAQPWTCSLLRR298 pKa = 11.84RR299 pKa = 11.84HH300 pKa = 6.58LMWLHH305 pKa = 5.99RR306 pKa = 11.84HH307 pKa = 6.3LDD309 pKa = 3.64NSSLVQMSNHH319 pKa = 5.13VLAMLGPKK327 pKa = 9.86SVISSDD333 pKa = 3.45YY334 pKa = 10.75KK335 pKa = 10.91VFDD338 pKa = 4.47IIEE341 pKa = 4.34GGMASGWKK349 pKa = 8.41LTILGNSWMQLILHH363 pKa = 6.79HH364 pKa = 6.64LAQLRR369 pKa = 11.84AGTTGPPPLCMGDD382 pKa = 3.72DD383 pKa = 4.82TIQTTMPDD391 pKa = 3.7EE392 pKa = 4.38YY393 pKa = 10.25WNEE396 pKa = 3.67LAATGCILKK405 pKa = 10.05EE406 pKa = 4.3VNNHH410 pKa = 6.22LDD412 pKa = 3.34GSPYY416 pKa = 10.09EE417 pKa = 4.05FCGLHH422 pKa = 6.06FGEE425 pKa = 4.9EE426 pKa = 4.6FYY428 pKa = 11.19EE429 pKa = 3.88PSYY432 pKa = 10.44EE433 pKa = 4.2GKK435 pKa = 10.04HH436 pKa = 5.46SFLLRR441 pKa = 11.84FLEE444 pKa = 4.43PEE446 pKa = 4.27LFRR449 pKa = 11.84DD450 pKa = 4.15VMISYY455 pKa = 10.44QLLYY459 pKa = 11.06LFDD462 pKa = 4.38PVKK465 pKa = 10.79LLALRR470 pKa = 11.84RR471 pKa = 11.84WLIISGLQDD480 pKa = 3.32ALIDD484 pKa = 3.63VDD486 pKa = 3.01AWQYY490 pKa = 10.97RR491 pKa = 11.84LRR493 pKa = 11.84GLSRR497 pKa = 11.84WPLNPKK503 pKa = 9.68PRR505 pKa = 11.84ILL507 pKa = 4.11

Molecular weight: 57.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEC0|A0A1L3KEC0_9VIRU RNA-directed RNA polymerase OS=Beihai shrimp virus 4 OX=1922670 PE=4 SV=1
MM1 pKa = 7.08KK2 pKa = 9.97TDD4 pKa = 4.32FANWTWISSQLANIIHH20 pKa = 6.15PQCDD24 pKa = 3.66LPKK27 pKa = 10.1PADD30 pKa = 4.64LIQIAILIAVILWACAKK47 pKa = 9.76TDD49 pKa = 3.94LASLLVSLLKK59 pKa = 10.88SSWNRR64 pKa = 11.84FVEE67 pKa = 4.37AQSDD71 pKa = 4.54LIRR74 pKa = 11.84WIQKK78 pKa = 8.41LAPTLLLMTNILIEE92 pKa = 4.14GVPNFWYY99 pKa = 8.54ITIAAQIILLVVGFGVVSVMPQAKK123 pKa = 9.8LIPMLEE129 pKa = 4.15LQAVLPTSPFMDD141 pKa = 4.91DD142 pKa = 3.11KK143 pKa = 11.76SKK145 pKa = 10.98VPVVAVANANGDD157 pKa = 3.34GSHH160 pKa = 6.83IVGQAVRR167 pKa = 11.84IGTLKK172 pKa = 10.66GKK174 pKa = 10.01AAFATVAHH182 pKa = 5.91VAKK185 pKa = 10.31ALTGTPYY192 pKa = 10.95LVNLDD197 pKa = 3.57EE198 pKa = 4.42NVAVKK203 pKa = 10.54VLEE206 pKa = 3.96VVICVGVPDD215 pKa = 5.04AALLLCDD222 pKa = 4.25PKK224 pKa = 10.89KK225 pKa = 10.57VSQLGGIAKK234 pKa = 8.03TAQISCGAKK243 pKa = 8.85PAVLHH248 pKa = 5.07SHH250 pKa = 6.03GLKK253 pKa = 10.18ISTLSSLKK261 pKa = 10.41ACSKK265 pKa = 11.38ANVEE269 pKa = 4.27NNGNCTFVYY278 pKa = 10.23YY279 pKa = 10.69GSALPGFSGGPVSVLGRR296 pKa = 11.84VVGIHH301 pKa = 6.1TGATGATSSGYY312 pKa = 10.88LIDD315 pKa = 6.0GLLAWMRR322 pKa = 11.84RR323 pKa = 11.84FEE325 pKa = 4.07ARR327 pKa = 11.84AGRR330 pKa = 11.84VVMEE334 pKa = 4.18EE335 pKa = 4.14HH336 pKa = 7.27SPGAWMQKK344 pKa = 10.54LLDD347 pKa = 4.08RR348 pKa = 11.84DD349 pKa = 3.68EE350 pKa = 6.33DD351 pKa = 3.55VDD353 pKa = 3.53QYY355 pKa = 11.78YY356 pKa = 11.03DD357 pKa = 3.36FEE359 pKa = 4.88EE360 pKa = 4.61FVEE363 pKa = 4.46PNHH366 pKa = 7.0DD367 pKa = 3.46RR368 pKa = 11.84FLRR371 pKa = 11.84VSDD374 pKa = 3.51RR375 pKa = 11.84KK376 pKa = 8.05TGKK379 pKa = 10.4VYY381 pKa = 10.57LYY383 pKa = 10.93DD384 pKa = 4.18EE385 pKa = 5.79DD386 pKa = 5.8DD387 pKa = 4.46VDD389 pKa = 5.43DD390 pKa = 4.65EE391 pKa = 5.0LYY393 pKa = 11.17KK394 pKa = 10.94LALKK398 pKa = 10.48RR399 pKa = 11.84AGNTRR404 pKa = 11.84TRR406 pKa = 11.84TEE408 pKa = 3.84FEE410 pKa = 4.18LNSFKK415 pKa = 10.82KK416 pKa = 9.85HH417 pKa = 5.99APTPDD422 pKa = 2.97EE423 pKa = 4.37PVNYY427 pKa = 9.98DD428 pKa = 3.26YY429 pKa = 11.63VLRR432 pKa = 11.84GQQAMARR439 pKa = 11.84YY440 pKa = 8.02SAVVATLSRR449 pKa = 11.84SIMALQTVLGQVRR462 pKa = 11.84KK463 pKa = 9.73EE464 pKa = 3.99PSQTAQVGVDD474 pKa = 3.0VNAFNEE480 pKa = 4.45FAHH483 pKa = 6.56RR484 pKa = 11.84MSNVGVAMSQRR495 pKa = 11.84LVEE498 pKa = 4.04ICRR501 pKa = 11.84LGVTDD506 pKa = 4.72VVDD509 pKa = 3.68KK510 pKa = 11.32NRR512 pKa = 11.84AEE514 pKa = 4.18RR515 pKa = 11.84WRR517 pKa = 11.84ARR519 pKa = 11.84HH520 pKa = 5.88PSPPVSTEE528 pKa = 3.3TRR530 pKa = 11.84EE531 pKa = 3.95EE532 pKa = 4.0LRR534 pKa = 11.84RR535 pKa = 11.84MEE537 pKa = 4.34LALEE541 pKa = 3.96EE542 pKa = 5.01AYY544 pKa = 11.05NAMEE548 pKa = 4.3QIPVRR553 pKa = 11.84GDD555 pKa = 3.21DD556 pKa = 4.9DD557 pKa = 5.35FEE559 pKa = 4.35QHH561 pKa = 6.28TSIFCNSLQARR572 pKa = 11.84YY573 pKa = 8.96GVKK576 pKa = 10.38LGTEE580 pKa = 3.88FHH582 pKa = 6.81GDD584 pKa = 3.06SFLEE588 pKa = 3.91EE589 pKa = 4.18RR590 pKa = 11.84FASTSMTEE598 pKa = 3.99STKK601 pKa = 10.27MSTQPTPKK609 pKa = 9.83EE610 pKa = 3.89EE611 pKa = 4.17SASRR615 pKa = 11.84SQTSFSKK622 pKa = 10.8LSPYY626 pKa = 10.4PNLTDD631 pKa = 3.3GHH633 pKa = 5.64QSEE636 pKa = 5.13EE637 pKa = 4.6KK638 pKa = 10.23PFQLPSPITKK648 pKa = 9.32TDD650 pKa = 2.94AEE652 pKa = 4.24LRR654 pKa = 11.84RR655 pKa = 11.84NALEE659 pKa = 4.28NPTKK663 pKa = 10.63EE664 pKa = 4.21SLEE667 pKa = 4.06QSKK670 pKa = 7.79QQCKK674 pKa = 8.51EE675 pKa = 3.38AMRR678 pKa = 11.84NLEE681 pKa = 4.1LASPVPQEE689 pKa = 4.15TGQKK693 pKa = 8.78DD694 pKa = 3.83TLTSSPQIYY703 pKa = 8.3QQHH706 pKa = 5.26RR707 pKa = 11.84VEE709 pKa = 5.11LSDD712 pKa = 3.8HH713 pKa = 6.34NGVPVKK719 pKa = 10.55NIKK722 pKa = 10.27INMLDD727 pKa = 3.61RR728 pKa = 11.84NSNPNKK734 pKa = 9.94NRR736 pKa = 11.84PFQCATCKK744 pKa = 10.22TPLDD748 pKa = 3.73AGKK751 pKa = 10.11VLKK754 pKa = 10.33HH755 pKa = 6.01RR756 pKa = 11.84CPKK759 pKa = 10.22IGTGQSTMM767 pKa = 3.8

Molecular weight: 84.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1274

507

767

637.0

71.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.3 ± 1.053

1.491 ± 0.164

5.573 ± 0.394

6.279 ± 0.228

3.14 ± 0.009

6.515 ± 0.628

2.59 ± 0.302

4.867 ± 0.35

5.259 ± 0.491

10.361 ± 0.681

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.747 ± 0.218

4.003 ± 0.347

5.416 ± 0.048

4.945 ± 0.098

5.416 ± 0.162

6.986 ± 0.36

5.965 ± 0.131

6.75 ± 1.286

1.962 ± 0.637

2.433 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski