Candidatus Portiera aleyrodidarum MED (Bemisia tabaci)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Zymobacter group; Candidatus Portiera; Candidatus Portiera aleyrodidarum

Average proteome isoelectric point is 8.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 254 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A249DZX6|A0A249DZX6_9GAMM RNA polymerase sigma factor RpoD OS=Candidatus Portiera aleyrodidarum MED (Bemisia tabaci) OX=1163752 GN=rpoD PE=3 SV=1
MM1 pKa = 8.17RR2 pKa = 11.84DD3 pKa = 3.11TFLIDD8 pKa = 3.98AFLLLWFYY16 pKa = 10.11FYY18 pKa = 10.51IYY20 pKa = 10.26FFYY23 pKa = 10.83VLCFMFYY30 pKa = 9.87VVVVVVDD37 pKa = 3.11ICILFFIFDD46 pKa = 4.38LGCYY50 pKa = 8.92LMCMFYY56 pKa = 10.62LWYY59 pKa = 8.89MYY61 pKa = 10.8FDD63 pKa = 2.87ICFRR67 pKa = 11.84YY68 pKa = 9.89

Molecular weight: 8.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A249E1J7|A0A249E1J7_9GAMM 30S ribosomal protein S16 OS=Candidatus Portiera aleyrodidarum MED (Bemisia tabaci) OX=1163752 GN=rpsP PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.62IKK11 pKa = 10.12IKK13 pKa = 10.23RR14 pKa = 11.84VNGFRR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84MSTKK25 pKa = 8.39IGRR28 pKa = 11.84RR29 pKa = 11.84IISNRR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.45KK37 pKa = 10.2KK38 pKa = 10.7RR39 pKa = 11.84NILCKK44 pKa = 10.49

Molecular weight: 5.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

254

0

254

78248

37

1372

308.1

35.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.444 ± 0.106

1.544 ± 0.065

3.799 ± 0.082

4.694 ± 0.165

4.607 ± 0.123

5.812 ± 0.144

1.838 ± 0.07

12.123 ± 0.155

11.003 ± 0.171

10.095 ± 0.15

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.989 ± 0.059

7.247 ± 0.147

2.702 ± 0.074

2.679 ± 0.074

4.021 ± 0.109

5.889 ± 0.09

5.111 ± 0.085

5.307 ± 0.109

0.787 ± 0.059

4.309 ± 0.104
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski