Amino acid dipepetide frequency for Streptococcus phage phiNJ3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.918AlaAla: 3.918 ± 0.941
0.429AlaCys: 0.429 ± 0.154
3.796AlaAsp: 3.796 ± 0.441
4.775AlaGlu: 4.775 ± 0.457
2.877AlaPhe: 2.877 ± 0.36
3.612AlaGly: 3.612 ± 0.414
0.429AlaHis: 0.429 ± 0.152
5.448AlaIle: 5.448 ± 0.715
5.632AlaLys: 5.632 ± 0.596
6.428AlaLeu: 6.428 ± 0.607
1.53AlaMet: 1.53 ± 0.223
3.122AlaAsn: 3.122 ± 0.64
1.469AlaPro: 1.469 ± 0.3
2.51AlaGln: 2.51 ± 0.603
2.694AlaArg: 2.694 ± 0.341
4.775AlaSer: 4.775 ± 0.523
3.551AlaThr: 3.551 ± 0.61
4.102AlaVal: 4.102 ± 0.613
0.918AlaTrp: 0.918 ± 0.319
2.816AlaTyr: 2.816 ± 0.462
0.0AlaXaa: 0.0 ± 0.0
Cys
0.612CysAla: 0.612 ± 0.23
0.306CysCys: 0.306 ± 0.125
0.49CysAsp: 0.49 ± 0.159
0.429CysGlu: 0.429 ± 0.16
0.429CysPhe: 0.429 ± 0.18
0.673CysGly: 0.673 ± 0.223
0.184CysHis: 0.184 ± 0.103
0.306CysIle: 0.306 ± 0.129
0.857CysLys: 0.857 ± 0.27
0.673CysLeu: 0.673 ± 0.208
0.122CysMet: 0.122 ± 0.085
0.367CysAsn: 0.367 ± 0.161
0.49CysPro: 0.49 ± 0.161
0.673CysGln: 0.673 ± 0.219
0.796CysArg: 0.796 ± 0.23
0.673CysSer: 0.673 ± 0.243
0.122CysThr: 0.122 ± 0.082
0.735CysVal: 0.735 ± 0.249
0.0CysTrp: 0.0 ± 0.0
0.612CysTyr: 0.612 ± 0.241
0.0CysXaa: 0.0 ± 0.0
Asp
2.938AspAla: 2.938 ± 0.419
0.796AspCys: 0.796 ± 0.274
2.938AspAsp: 2.938 ± 0.718
5.142AspGlu: 5.142 ± 0.759
3.796AspPhe: 3.796 ± 0.503
4.653AspGly: 4.653 ± 0.466
0.918AspHis: 0.918 ± 0.236
4.836AspIle: 4.836 ± 0.48
3.857AspLys: 3.857 ± 0.49
4.346AspLeu: 4.346 ± 0.631
1.959AspMet: 1.959 ± 0.33
2.877AspAsn: 2.877 ± 0.48
1.53AspPro: 1.53 ± 0.327
1.775AspGln: 1.775 ± 0.345
2.877AspArg: 2.877 ± 0.555
3.0AspSer: 3.0 ± 0.534
2.51AspThr: 2.51 ± 0.396
3.489AspVal: 3.489 ± 0.429
0.735AspTrp: 0.735 ± 0.213
2.938AspTyr: 2.938 ± 0.459
0.0AspXaa: 0.0 ± 0.0
Glu
3.979GluAla: 3.979 ± 0.453
0.49GluCys: 0.49 ± 0.178
4.469GluAsp: 4.469 ± 0.533
6.061GluGlu: 6.061 ± 0.839
2.204GluPhe: 2.204 ± 0.384
4.653GluGly: 4.653 ± 0.511
1.163GluHis: 1.163 ± 0.282
5.755GluIle: 5.755 ± 0.488
6.367GluLys: 6.367 ± 0.694
7.713GluLeu: 7.713 ± 0.681
2.51GluMet: 2.51 ± 0.413
3.796GluAsn: 3.796 ± 0.699
1.592GluPro: 1.592 ± 0.405
4.346GluGln: 4.346 ± 0.332
3.367GluArg: 3.367 ± 0.447
3.857GluSer: 3.857 ± 0.454
4.653GluThr: 4.653 ± 0.586
4.224GluVal: 4.224 ± 0.485
0.612GluTrp: 0.612 ± 0.201
2.326GluTyr: 2.326 ± 0.484
0.0GluXaa: 0.0 ± 0.0
Phe
2.143PheAla: 2.143 ± 0.37
0.551PheCys: 0.551 ± 0.179
2.632PheAsp: 2.632 ± 0.453
3.367PheGlu: 3.367 ± 0.443
1.653PhePhe: 1.653 ± 0.287
2.449PheGly: 2.449 ± 0.325
1.041PheHis: 1.041 ± 0.248
2.755PheIle: 2.755 ± 0.415
3.673PheLys: 3.673 ± 0.599
3.122PheLeu: 3.122 ± 0.428
0.918PheMet: 0.918 ± 0.219
1.898PheAsn: 1.898 ± 0.292
0.796PhePro: 0.796 ± 0.228
1.653PheGln: 1.653 ± 0.278
1.775PheArg: 1.775 ± 0.421
2.081PheSer: 2.081 ± 0.368
2.143PheThr: 2.143 ± 0.362
2.326PheVal: 2.326 ± 0.38
0.612PheTrp: 0.612 ± 0.199
2.081PheTyr: 2.081 ± 0.373
0.0PheXaa: 0.0 ± 0.0
Gly
3.428GlyAla: 3.428 ± 0.517
0.367GlyCys: 0.367 ± 0.126
3.489GlyAsp: 3.489 ± 0.504
3.734GlyGlu: 3.734 ± 0.465
2.51GlyPhe: 2.51 ± 0.376
3.489GlyGly: 3.489 ± 0.468
2.265GlyHis: 2.265 ± 0.354
6.122GlyIle: 6.122 ± 0.756
4.469GlyLys: 4.469 ± 0.554
5.204GlyLeu: 5.204 ± 0.604
1.775GlyMet: 1.775 ± 0.397
3.183GlyAsn: 3.183 ± 0.449
0.612GlyPro: 0.612 ± 0.178
2.938GlyGln: 2.938 ± 0.459
3.367GlyArg: 3.367 ± 0.368
3.489GlySer: 3.489 ± 0.46
4.224GlyThr: 4.224 ± 0.509
3.796GlyVal: 3.796 ± 0.457
0.551GlyTrp: 0.551 ± 0.142
3.0GlyTyr: 3.0 ± 0.385
0.0GlyXaa: 0.0 ± 0.0
His
0.796HisAla: 0.796 ± 0.219
0.306HisCys: 0.306 ± 0.125
1.347HisAsp: 1.347 ± 0.3
1.163HisGlu: 1.163 ± 0.272
1.163HisPhe: 1.163 ± 0.246
1.469HisGly: 1.469 ± 0.312
0.49HisHis: 0.49 ± 0.189
1.653HisIle: 1.653 ± 0.304
1.163HisLys: 1.163 ± 0.26
1.714HisLeu: 1.714 ± 0.309
0.429HisMet: 0.429 ± 0.215
0.735HisAsn: 0.735 ± 0.227
1.163HisPro: 1.163 ± 0.25
0.673HisGln: 0.673 ± 0.181
0.979HisArg: 0.979 ± 0.202
0.796HisSer: 0.796 ± 0.228
1.224HisThr: 1.224 ± 0.318
1.286HisVal: 1.286 ± 0.259
0.245HisTrp: 0.245 ± 0.106
0.673HisTyr: 0.673 ± 0.262
0.0HisXaa: 0.0 ± 0.0
Ile
4.775IleAla: 4.775 ± 0.373
0.612IleCys: 0.612 ± 0.218
5.51IleAsp: 5.51 ± 0.588
4.653IleGlu: 4.653 ± 0.663
1.959IlePhe: 1.959 ± 0.382
4.836IleGly: 4.836 ± 0.621
1.286IleHis: 1.286 ± 0.242
3.796IleIle: 3.796 ± 0.495
4.897IleLys: 4.897 ± 0.539
6.122IleLeu: 6.122 ± 0.564
1.347IleMet: 1.347 ± 0.342
3.367IleAsn: 3.367 ± 0.362
2.816IlePro: 2.816 ± 0.478
2.877IleGln: 2.877 ± 0.374
3.183IleArg: 3.183 ± 0.489
5.632IleSer: 5.632 ± 1.094
4.591IleThr: 4.591 ± 0.7
4.714IleVal: 4.714 ± 0.693
1.041IleTrp: 1.041 ± 0.309
2.265IleTyr: 2.265 ± 0.416
0.0IleXaa: 0.0 ± 0.0
Lys
5.755LysAla: 5.755 ± 0.469
0.367LysCys: 0.367 ± 0.149
3.979LysAsp: 3.979 ± 0.531
4.959LysGlu: 4.959 ± 0.573
2.877LysPhe: 2.877 ± 0.447
4.04LysGly: 4.04 ± 0.448
1.53LysHis: 1.53 ± 0.333
5.387LysIle: 5.387 ± 0.579
4.653LysLys: 4.653 ± 0.639
6.612LysLeu: 6.612 ± 0.573
1.837LysMet: 1.837 ± 0.249
3.183LysAsn: 3.183 ± 0.403
2.265LysPro: 2.265 ± 0.369
3.428LysGln: 3.428 ± 0.413
4.04LysArg: 4.04 ± 0.533
5.142LysSer: 5.142 ± 0.632
4.469LysThr: 4.469 ± 0.515
4.775LysVal: 4.775 ± 0.576
1.102LysTrp: 1.102 ± 0.3
2.51LysTyr: 2.51 ± 0.405
0.0LysXaa: 0.0 ± 0.0
Leu
5.938LeuAla: 5.938 ± 0.678
0.551LeuCys: 0.551 ± 0.169
5.326LeuAsp: 5.326 ± 0.591
7.652LeuGlu: 7.652 ± 0.736
3.489LeuPhe: 3.489 ± 0.496
5.265LeuGly: 5.265 ± 0.549
1.53LeuHis: 1.53 ± 0.288
5.204LeuIle: 5.204 ± 0.603
7.101LeuLys: 7.101 ± 0.745
8.142LeuLeu: 8.142 ± 0.881
1.653LeuMet: 1.653 ± 0.241
4.408LeuAsn: 4.408 ± 0.59
3.489LeuPro: 3.489 ± 0.571
3.612LeuGln: 3.612 ± 0.407
3.551LeuArg: 3.551 ± 0.388
7.469LeuSer: 7.469 ± 0.601
6.489LeuThr: 6.489 ± 0.646
5.938LeuVal: 5.938 ± 0.738
0.429LeuTrp: 0.429 ± 0.145
3.551LeuTyr: 3.551 ± 0.57
0.0LeuXaa: 0.0 ± 0.0
Met
2.265MetAla: 2.265 ± 0.395
0.184MetCys: 0.184 ± 0.099
1.53MetAsp: 1.53 ± 0.3
1.775MetGlu: 1.775 ± 0.362
0.918MetPhe: 0.918 ± 0.204
1.898MetGly: 1.898 ± 0.361
0.122MetHis: 0.122 ± 0.077
1.653MetIle: 1.653 ± 0.315
1.775MetLys: 1.775 ± 0.351
1.714MetLeu: 1.714 ± 0.288
0.796MetMet: 0.796 ± 0.216
1.102MetAsn: 1.102 ± 0.276
0.673MetPro: 0.673 ± 0.204
0.857MetGln: 0.857 ± 0.236
1.102MetArg: 1.102 ± 0.225
2.143MetSer: 2.143 ± 0.329
1.653MetThr: 1.653 ± 0.4
1.469MetVal: 1.469 ± 0.324
0.184MetTrp: 0.184 ± 0.104
0.429MetTyr: 0.429 ± 0.149
0.0MetXaa: 0.0 ± 0.0
Asn
4.346AsnAla: 4.346 ± 0.641
0.367AsnCys: 0.367 ± 0.162
2.571AsnAsp: 2.571 ± 0.359
2.816AsnGlu: 2.816 ± 0.512
1.775AsnPhe: 1.775 ± 0.292
4.591AsnGly: 4.591 ± 0.519
1.347AsnHis: 1.347 ± 0.214
2.51AsnIle: 2.51 ± 0.297
2.816AsnLys: 2.816 ± 0.423
4.53AsnLeu: 4.53 ± 0.699
0.918AsnMet: 0.918 ± 0.238
1.959AsnAsn: 1.959 ± 0.29
2.326AsnPro: 2.326 ± 0.364
2.265AsnGln: 2.265 ± 0.372
2.081AsnArg: 2.081 ± 0.368
2.755AsnSer: 2.755 ± 0.332
2.388AsnThr: 2.388 ± 0.452
2.265AsnVal: 2.265 ± 0.459
1.041AsnTrp: 1.041 ± 0.264
1.347AsnTyr: 1.347 ± 0.254
0.0AsnXaa: 0.0 ± 0.0
Pro
1.592ProAla: 1.592 ± 0.337
0.367ProCys: 0.367 ± 0.132
1.837ProAsp: 1.837 ± 0.404
2.388ProGlu: 2.388 ± 0.354
1.286ProPhe: 1.286 ± 0.288
0.857ProGly: 0.857 ± 0.284
0.735ProHis: 0.735 ± 0.198
1.775ProIle: 1.775 ± 0.318
2.081ProLys: 2.081 ± 0.414
3.122ProLeu: 3.122 ± 0.543
0.735ProMet: 0.735 ± 0.192
1.469ProAsn: 1.469 ± 0.305
0.979ProPro: 0.979 ± 0.242
1.041ProGln: 1.041 ± 0.237
1.959ProArg: 1.959 ± 0.246
2.388ProSer: 2.388 ± 0.459
2.265ProThr: 2.265 ± 0.415
2.449ProVal: 2.449 ± 0.439
0.306ProTrp: 0.306 ± 0.121
1.53ProTyr: 1.53 ± 0.327
0.0ProXaa: 0.0 ± 0.0
Gln
3.918GlnAla: 3.918 ± 0.509
0.429GlnCys: 0.429 ± 0.166
1.775GlnAsp: 1.775 ± 0.334
3.367GlnGlu: 3.367 ± 0.358
1.714GlnPhe: 1.714 ± 0.254
1.959GlnGly: 1.959 ± 0.303
0.673GlnHis: 0.673 ± 0.166
2.632GlnIle: 2.632 ± 0.466
3.245GlnLys: 3.245 ± 0.409
4.469GlnLeu: 4.469 ± 0.424
1.163GlnMet: 1.163 ± 0.295
1.959GlnAsn: 1.959 ± 0.324
1.347GlnPro: 1.347 ± 0.324
1.714GlnGln: 1.714 ± 0.359
1.898GlnArg: 1.898 ± 0.444
2.755GlnSer: 2.755 ± 0.466
2.938GlnThr: 2.938 ± 0.65
3.612GlnVal: 3.612 ± 0.538
0.857GlnTrp: 0.857 ± 0.252
1.041GlnTyr: 1.041 ± 0.282
0.0GlnXaa: 0.0 ± 0.0
Arg
2.388ArgAla: 2.388 ± 0.39
0.673ArgCys: 0.673 ± 0.203
2.632ArgAsp: 2.632 ± 0.394
3.122ArgGlu: 3.122 ± 0.404
1.898ArgPhe: 1.898 ± 0.333
2.449ArgGly: 2.449 ± 0.28
0.796ArgHis: 0.796 ± 0.241
3.245ArgIle: 3.245 ± 0.518
4.346ArgLys: 4.346 ± 0.6
4.653ArgLeu: 4.653 ± 0.459
0.673ArgMet: 0.673 ± 0.206
2.326ArgAsn: 2.326 ± 0.354
1.408ArgPro: 1.408 ± 0.303
2.755ArgGln: 2.755 ± 0.376
1.959ArgArg: 1.959 ± 0.374
2.938ArgSer: 2.938 ± 0.4
2.755ArgThr: 2.755 ± 0.52
3.367ArgVal: 3.367 ± 0.377
0.918ArgTrp: 0.918 ± 0.238
1.592ArgTyr: 1.592 ± 0.326
0.0ArgXaa: 0.0 ± 0.0
Ser
4.469SerAla: 4.469 ± 0.516
0.551SerCys: 0.551 ± 0.175
4.224SerAsp: 4.224 ± 0.449
4.775SerGlu: 4.775 ± 0.562
2.816SerPhe: 2.816 ± 0.525
4.653SerGly: 4.653 ± 0.556
1.53SerHis: 1.53 ± 0.292
5.142SerIle: 5.142 ± 0.564
4.224SerLys: 4.224 ± 0.56
5.448SerLeu: 5.448 ± 0.572
1.775SerMet: 1.775 ± 0.252
3.245SerAsn: 3.245 ± 0.621
2.326SerPro: 2.326 ± 0.376
2.816SerGln: 2.816 ± 0.607
3.245SerArg: 3.245 ± 0.439
4.714SerSer: 4.714 ± 0.617
4.408SerThr: 4.408 ± 0.549
3.918SerVal: 3.918 ± 0.436
0.918SerTrp: 0.918 ± 0.22
2.755SerTyr: 2.755 ± 0.464
0.0SerXaa: 0.0 ± 0.0
Thr
4.408ThrAla: 4.408 ± 0.59
0.245ThrCys: 0.245 ± 0.112
2.694ThrAsp: 2.694 ± 0.494
5.081ThrGlu: 5.081 ± 0.441
2.143ThrPhe: 2.143 ± 0.444
4.224ThrGly: 4.224 ± 0.578
1.041ThrHis: 1.041 ± 0.215
4.836ThrIle: 4.836 ± 0.586
4.469ThrLys: 4.469 ± 0.566
5.387ThrLeu: 5.387 ± 0.523
1.347ThrMet: 1.347 ± 0.297
2.632ThrAsn: 2.632 ± 0.444
2.204ThrPro: 2.204 ± 0.426
1.959ThrGln: 1.959 ± 0.695
2.143ThrArg: 2.143 ± 0.391
4.836ThrSer: 4.836 ± 0.727
5.081ThrThr: 5.081 ± 0.811
5.51ThrVal: 5.51 ± 0.643
0.796ThrTrp: 0.796 ± 0.239
2.388ThrTyr: 2.388 ± 0.493
0.0ThrXaa: 0.0 ± 0.0
Val
3.796ValAla: 3.796 ± 0.488
0.979ValCys: 0.979 ± 0.299
3.245ValAsp: 3.245 ± 0.464
4.959ValGlu: 4.959 ± 0.546
2.143ValPhe: 2.143 ± 0.367
3.551ValGly: 3.551 ± 0.564
1.163ValHis: 1.163 ± 0.215
4.04ValIle: 4.04 ± 0.567
4.224ValLys: 4.224 ± 0.492
6.795ValLeu: 6.795 ± 0.525
1.224ValMet: 1.224 ± 0.254
2.694ValAsn: 2.694 ± 0.419
2.326ValPro: 2.326 ± 0.379
2.816ValGln: 2.816 ± 0.318
3.367ValArg: 3.367 ± 0.503
4.653ValSer: 4.653 ± 0.721
4.53ValThr: 4.53 ± 0.439
3.183ValVal: 3.183 ± 0.461
1.102ValTrp: 1.102 ± 0.232
2.632ValTyr: 2.632 ± 0.525
0.0ValXaa: 0.0 ± 0.0
Trp
1.163TrpAla: 1.163 ± 0.289
0.184TrpCys: 0.184 ± 0.117
0.612TrpAsp: 0.612 ± 0.183
1.163TrpGlu: 1.163 ± 0.259
0.673TrpPhe: 0.673 ± 0.219
0.735TrpGly: 0.735 ± 0.198
0.184TrpHis: 0.184 ± 0.099
0.673TrpIle: 0.673 ± 0.167
0.796TrpLys: 0.796 ± 0.234
0.918TrpLeu: 0.918 ± 0.247
0.49TrpMet: 0.49 ± 0.163
1.041TrpAsn: 1.041 ± 0.313
0.061TrpPro: 0.061 ± 0.055
0.735TrpGln: 0.735 ± 0.23
0.429TrpArg: 0.429 ± 0.233
0.735TrpSer: 0.735 ± 0.301
0.979TrpThr: 0.979 ± 0.305
0.612TrpVal: 0.612 ± 0.184
0.245TrpTrp: 0.245 ± 0.133
0.429TrpTyr: 0.429 ± 0.129
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.265TyrAla: 2.265 ± 0.376
0.796TyrCys: 0.796 ± 0.273
2.816TyrAsp: 2.816 ± 0.535
2.755TyrGlu: 2.755 ± 0.455
1.347TyrPhe: 1.347 ± 0.283
1.959TyrGly: 1.959 ± 0.348
1.041TyrHis: 1.041 ± 0.276
2.51TyrIle: 2.51 ± 0.428
2.265TyrLys: 2.265 ± 0.365
3.673TyrLeu: 3.673 ± 0.48
0.979TyrMet: 0.979 ± 0.28
1.653TyrAsn: 1.653 ± 0.291
1.286TyrPro: 1.286 ± 0.266
2.02TyrGln: 2.02 ± 0.349
2.081TyrArg: 2.081 ± 0.308
3.122TyrSer: 3.122 ± 0.532
2.449TyrThr: 2.449 ± 0.417
1.653TyrVal: 1.653 ± 0.309
0.245TyrTrp: 0.245 ± 0.122
1.102TyrTyr: 1.102 ± 0.324
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (16336 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski