Streptococcus phage Javan258

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AVJ8|A0A4D6AVJ8_9CAUD Cro/CI family transcriptional regulator OS=Streptococcus phage Javan258 OX=2548076 GN=Javan258_0056 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.25KK3 pKa = 10.41YY4 pKa = 10.66DD5 pKa = 3.87GEE7 pKa = 4.14LKK9 pKa = 10.24KK10 pKa = 11.1YY11 pKa = 7.73YY12 pKa = 10.09VCGVIDD18 pKa = 5.65DD19 pKa = 4.4YY20 pKa = 11.59PEE22 pKa = 4.12KK23 pKa = 10.71QLDD26 pKa = 3.42LGMEE30 pKa = 4.22VMANNPYY37 pKa = 8.37MAAIIAHH44 pKa = 6.65KK45 pKa = 10.46SIEE48 pKa = 4.35DD49 pKa = 3.84DD50 pKa = 5.03FMLDD54 pKa = 4.26DD55 pKa = 3.99PTITDD60 pKa = 3.15VDD62 pKa = 3.52ILEE65 pKa = 4.52VQDD68 pKa = 3.5GDD70 pKa = 4.11YY71 pKa = 11.51

Molecular weight: 8.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B081|A0A4D6B081_9CAUD Terminase small subunit OS=Streptococcus phage Javan258 OX=2548076 GN=Javan258_0025 PE=4 SV=1
MM1 pKa = 7.56AKK3 pKa = 10.44GFVTHH8 pKa = 6.56AHH10 pKa = 5.85VDD12 pKa = 3.47LGGVTKK18 pKa = 10.37KK19 pKa = 10.62VSPEE23 pKa = 3.25AVRR26 pKa = 11.84RR27 pKa = 11.84GRR29 pKa = 11.84QAMANQILLDD39 pKa = 3.73TNAYY43 pKa = 9.61VPLRR47 pKa = 11.84DD48 pKa = 3.31GALRR52 pKa = 11.84ASATIGLQGEE62 pKa = 4.5TVNWNTVYY70 pKa = 10.92ARR72 pKa = 11.84AHH74 pKa = 6.21FYY76 pKa = 8.37GTNGIVTFRR85 pKa = 11.84EE86 pKa = 4.18YY87 pKa = 7.87TTPGTGKK94 pKa = 9.57NWYY97 pKa = 9.18VKK99 pKa = 10.61AKK101 pKa = 10.43NKK103 pKa = 10.22HH104 pKa = 4.65LDD106 pKa = 2.99KK107 pKa = 11.06WKK109 pKa = 9.38DD110 pKa = 3.43TAVKK114 pKa = 10.81AMGLKK119 pKa = 10.15

Molecular weight: 13.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

12272

37

1268

208.0

23.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.676 ± 0.917

0.375 ± 0.056

6.511 ± 0.41

6.209 ± 0.59

3.968 ± 0.182

6.829 ± 0.627

1.475 ± 0.173

6.959 ± 0.205

7.969 ± 0.399

7.717 ± 0.429

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.526 ± 0.259

6.16 ± 0.423

2.746 ± 0.197

4.058 ± 0.258

3.765 ± 0.286

6.494 ± 0.496

6.91 ± 0.442

6.348 ± 0.227

1.133 ± 0.136

4.172 ± 0.422
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski