Mycobacterium phage Soul22
Average proteome isoelectric point is 6.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 102 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7D5FNK8|A0A7D5FNK8_9CAUD CRO protein OS=Mycobacterium phage Soul22 OX=2743996 GN=50 PE=4 SV=1
MM1 pKa = 7.4 LLDD4 pKa = 3.67 GTFDD8 pKa = 4.48 LDD10 pKa = 3.48 SDD12 pKa = 4.11 TFKK15 pKa = 11.17 VALVTSSSNIGASSTTWSGVTGEE38 pKa = 4.12 VSNGNGYY45 pKa = 6.18 TTGGVSVTLTLTGTTSVAVSFATNPVWTASGSGITARR82 pKa = 11.84 WAVLYY87 pKa = 9.06 EE88 pKa = 3.88 VSGNVLAYY96 pKa = 10.61 VLLDD100 pKa = 3.61 TTGGGQDD107 pKa = 3.4 VSVSAGNTLTIDD119 pKa = 3.36 SDD121 pKa = 4.13 GTPSPVFTLSS131 pKa = 2.89
Molecular weight: 13.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.898
IPC2_protein 3.503
IPC_protein 3.414
Toseland 3.21
ProMoST 3.656
Dawson 3.452
Bjellqvist 3.63
Wikipedia 3.478
Rodwell 3.261
Grimsley 3.134
Solomon 3.389
Lehninger 3.35
Nozaki 3.617
DTASelect 3.834
Thurlkill 3.325
EMBOSS 3.478
Sillero 3.554
Patrickios 1.812
IPC_peptide 3.376
IPC2_peptide 3.503
IPC2.peptide.svr19 3.687
Protein with the highest isoelectric point:
>tr|A0A7D5JQI1|A0A7D5JQI1_9CAUD Excise OS=Mycobacterium phage Soul22 OX=2743996 GN=54 PE=4 SV=1
MM1 pKa = 7.46 TGPGARR7 pKa = 11.84 QRR9 pKa = 11.84 IPAPNPITEE18 pKa = 4.64 LSRR21 pKa = 11.84 EE22 pKa = 4.02 GLAVDD27 pKa = 4.22 ATQEE31 pKa = 3.52 QWRR34 pKa = 11.84 PVVGYY39 pKa = 9.66 EE40 pKa = 4.2 GMYY43 pKa = 10.11 EE44 pKa = 4.0 VSDD47 pKa = 3.91 LGRR50 pKa = 11.84 VRR52 pKa = 11.84 SVDD55 pKa = 2.96 RR56 pKa = 11.84 CVVTKK61 pKa = 10.55 SGPRR65 pKa = 11.84 TYY67 pKa = 10.39 RR68 pKa = 11.84 GRR70 pKa = 11.84 LLHH73 pKa = 5.53 QHH75 pKa = 6.62 PDD77 pKa = 2.75 GRR79 pKa = 11.84 GYY81 pKa = 11.11 LRR83 pKa = 11.84 ASLSRR88 pKa = 11.84 VGDD91 pKa = 3.61 KK92 pKa = 10.65 PRR94 pKa = 11.84 MFKK97 pKa = 9.06 VHH99 pKa = 6.88 RR100 pKa = 11.84 LVLEE104 pKa = 4.17 AFVGPRR110 pKa = 11.84 PGNLSGCHH118 pKa = 5.25 NNGINTDD125 pKa = 3.3 NRR127 pKa = 11.84 LEE129 pKa = 3.91 NLRR132 pKa = 11.84 WDD134 pKa = 3.55 SHH136 pKa = 4.99 TEE138 pKa = 3.56 NMLDD142 pKa = 3.6 VVRR145 pKa = 11.84 HH146 pKa = 5.38 GRR148 pKa = 11.84 HH149 pKa = 5.83 HH150 pKa = 5.07 YY151 pKa = 10.47 AKK153 pKa = 10.07 RR154 pKa = 11.84 DD155 pKa = 3.55 RR156 pKa = 11.84 CPKK159 pKa = 9.06 GHH161 pKa = 6.52 VFNEE165 pKa = 4.08 PNTLISPRR173 pKa = 11.84 GARR176 pKa = 11.84 VCRR179 pKa = 11.84 TCQKK183 pKa = 10.44 QYY185 pKa = 10.88 NDD187 pKa = 3.98 RR188 pKa = 11.84 YY189 pKa = 9.46 YY190 pKa = 10.02 QTACDD195 pKa = 3.81 EE196 pKa = 3.94 KK197 pKa = 10.6 RR198 pKa = 11.84 ARR200 pKa = 11.84 GWIPKK205 pKa = 9.63 RR206 pKa = 11.84 EE207 pKa = 3.94 RR208 pKa = 11.84 TKK210 pKa = 10.93 CPLGHH215 pKa = 6.7 EE216 pKa = 3.92 YY217 pKa = 10.61 DD218 pKa = 3.52 YY219 pKa = 11.34 FYY221 pKa = 11.65 VNKK224 pKa = 10.32 RR225 pKa = 11.84 NGKK228 pKa = 6.05 VTRR231 pKa = 11.84 HH232 pKa = 6.06 CKK234 pKa = 7.53 TCRR237 pKa = 11.84 AQNHH241 pKa = 5.53 RR242 pKa = 11.84 NFKK245 pKa = 9.89 KK246 pKa = 10.08 RR247 pKa = 11.84 RR248 pKa = 11.84 AGASCVV254 pKa = 3.28
Molecular weight: 29.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.336
IPC2_protein 9.516
IPC_protein 10.101
Toseland 10.248
ProMoST 9.999
Dawson 10.438
Bjellqvist 10.189
Wikipedia 10.643
Rodwell 10.657
Grimsley 10.511
Solomon 10.496
Lehninger 10.452
Nozaki 10.321
DTASelect 10.16
Thurlkill 10.306
EMBOSS 10.657
Sillero 10.379
Patrickios 10.233
IPC_peptide 10.496
IPC2_peptide 9.385
IPC2.peptide.svr19 8.48
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
102
0
102
17615
30
1183
172.7
18.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.701 ± 0.463
1.408 ± 0.17
6.404 ± 0.198
5.949 ± 0.353
3.037 ± 0.14
8.839 ± 0.597
2.231 ± 0.149
4.627 ± 0.18
3.633 ± 0.178
7.584 ± 0.296
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.112 ± 0.124
3.247 ± 0.182
5.876 ± 0.206
3.616 ± 0.174
6.852 ± 0.436
5.853 ± 0.267
6.341 ± 0.261
6.983 ± 0.266
2.288 ± 0.137
2.418 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here