Mycolicibacterium wolinskyi

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Act

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6894 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A132PGK2|A0A132PGK2_9MYCO DNA helicase OS=Mycolicibacterium wolinskyi OX=59750 GN=AFM11_25505 PE=3 SV=1
MM1 pKa = 7.6SGPNPPGPDD10 pKa = 3.28GSGSDD15 pKa = 5.51LPDD18 pKa = 3.39QQPGKK23 pKa = 9.85HH24 pKa = 5.86SAPEE28 pKa = 3.99EE29 pKa = 4.03PTQVAGTDD37 pKa = 3.63PNTGEE42 pKa = 4.2TEE44 pKa = 4.73FYY46 pKa = 9.98SQAYY50 pKa = 8.25SAPEE54 pKa = 3.93SEE56 pKa = 4.76QFTSGPYY63 pKa = 9.66VPAEE67 pKa = 4.24PGLYY71 pKa = 10.11DD72 pKa = 3.91YY73 pKa = 11.42DD74 pKa = 4.3SYY76 pKa = 12.23DD77 pKa = 3.54PVTEE81 pKa = 5.41LVDD84 pKa = 3.76QPPPPRR90 pKa = 11.84WPWVVGVTAIIAAIALVASVAVLVTRR116 pKa = 11.84DD117 pKa = 3.84DD118 pKa = 3.79DD119 pKa = 4.18TTNLATPQPSTTTSAPPFQDD139 pKa = 4.19EE140 pKa = 4.65ITTTTPPPPPPPPPPTSEE158 pKa = 4.44EE159 pKa = 4.16PPPPPPEE166 pKa = 4.02TVTVTEE172 pKa = 5.03PPPPPPPPPSSEE184 pKa = 4.25APPPPPATTTTPPPTTTTAPAGPRR208 pKa = 11.84QVTYY212 pKa = 11.02SVTGTKK218 pKa = 10.45APGDD222 pKa = 4.39IITITYY228 pKa = 10.34VDD230 pKa = 3.55GNGNRR235 pKa = 11.84RR236 pKa = 11.84TLRR239 pKa = 11.84NVYY242 pKa = 9.98IPWTFTMTPISNSDD256 pKa = 3.4VGSVEE261 pKa = 3.81ASSLFLVSRR270 pKa = 11.84LNCSITASDD279 pKa = 3.87GTVLSSNANNSAQTACC295 pKa = 4.32

Molecular weight: 30.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A132PC56|A0A132PC56_9MYCO GNAT family acetyltransferase OS=Mycolicibacterium wolinskyi OX=59750 GN=AFM11_33570 PE=4 SV=1
MM1 pKa = 7.57ALAGSAVAVGVAATQAHH18 pKa = 5.73PTSRR22 pKa = 11.84TRR24 pKa = 11.84TAITRR29 pKa = 11.84AKK31 pKa = 9.5IVRR34 pKa = 11.84RR35 pKa = 11.84HH36 pKa = 4.51VARR39 pKa = 11.84AHH41 pKa = 6.65ADD43 pKa = 3.11HH44 pKa = 6.71EE45 pKa = 5.16RR46 pKa = 11.84NVSRR50 pKa = 11.84RR51 pKa = 11.84NRR53 pKa = 11.84VFPTQAIHH61 pKa = 5.8VRR63 pKa = 11.84RR64 pKa = 11.84FNATAARR71 pKa = 11.84AEE73 pKa = 4.14GRR75 pKa = 11.84RR76 pKa = 11.84LTKK79 pKa = 10.31GYY81 pKa = 10.15RR82 pKa = 11.84KK83 pKa = 8.75QAKK86 pKa = 8.17SVRR89 pKa = 11.84KK90 pKa = 9.26RR91 pKa = 11.84RR92 pKa = 11.84RR93 pKa = 11.84NRR95 pKa = 11.84RR96 pKa = 3.2

Molecular weight: 10.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6894

0

6894

2192979

32

3669

318.1

34.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.096 ± 0.039

0.788 ± 0.009

6.311 ± 0.022

5.289 ± 0.024

3.084 ± 0.019

8.762 ± 0.024

2.187 ± 0.014

4.404 ± 0.021

2.208 ± 0.017

9.889 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.062 ± 0.014

2.241 ± 0.015

5.721 ± 0.027

2.974 ± 0.015

7.136 ± 0.03

5.316 ± 0.017

6.087 ± 0.02

8.785 ± 0.029

1.488 ± 0.011

2.17 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski