Polaribacter reichenbachii
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3418 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B8U4M2|A0A1B8U4M2_9FLAO Peptidase_S8 domain-containing protein OS=Polaribacter reichenbachii OX=996801 GN=LPB301_05110 PE=4 SV=1
MM1 pKa = 7.49 KK2 pKa = 10.44 SKK4 pKa = 10.85 LSVFIFILFIFYY16 pKa = 10.82 CKK18 pKa = 10.0 DD19 pKa = 3.06 VKK21 pKa = 11.06 AQLSKK26 pKa = 11.05 KK27 pKa = 10.04 HH28 pKa = 6.45 FIPPLTYY35 pKa = 10.53 AEE37 pKa = 4.47 EE38 pKa = 4.67 GNANPEE44 pKa = 3.76 SQYY47 pKa = 11.07 FYY49 pKa = 10.92 ISTPTNKK56 pKa = 9.95 DD57 pKa = 2.52 VSYY60 pKa = 9.75 TIKK63 pKa = 10.78 QIGQDD68 pKa = 3.32 NDD70 pKa = 2.84 ITGIVSSSNPQEE82 pKa = 3.95 IFIATGDD89 pKa = 3.59 SQLFVNANEE98 pKa = 4.26 TGTVHH103 pKa = 5.92 TNKK106 pKa = 10.34 GYY108 pKa = 10.12 IIEE111 pKa = 4.52 ATDD114 pKa = 3.52 VIYY117 pKa = 11.25 VSVRR121 pKa = 11.84 VLAGGGAQAGALVSKK136 pKa = 10.56 GSSALGTTFRR146 pKa = 11.84 AGMFTNEE153 pKa = 4.17 NPQSNYY159 pKa = 10.6 LNFVSVMASEE169 pKa = 5.52 DD170 pKa = 3.41 NTQITFDD177 pKa = 5.46 DD178 pKa = 3.76 ITPGLEE184 pKa = 3.57 IKK186 pKa = 10.55 NYY188 pKa = 9.26 TSTFPFSVVLNEE200 pKa = 3.97 GEE202 pKa = 4.38 SYY204 pKa = 10.54 IVATSADD211 pKa = 3.81 DD212 pKa = 4.22 VIVNRR217 pKa = 11.84 DD218 pKa = 3.44 GLIGTLISSDD228 pKa = 3.16 KK229 pKa = 10.99 NIVVNTGSANGSFHH243 pKa = 6.99 NGGGRR248 pKa = 11.84 DD249 pKa = 3.61 YY250 pKa = 11.43 GIDD253 pKa = 3.54 QIVGADD259 pKa = 4.08 KK260 pKa = 10.85 IGTDD264 pKa = 3.82 YY265 pKa = 11.29 IFVKK269 pKa = 10.85 GDD271 pKa = 3.51 GSNGWEE277 pKa = 3.82 NVLIVAHH284 pKa = 6.89 EE285 pKa = 4.71 DD286 pKa = 3.22 NTVVSRR292 pKa = 11.84 NGINSTINKK301 pKa = 9.71 GEE303 pKa = 3.95 YY304 pKa = 9.34 TLIEE308 pKa = 4.2 GDD310 pKa = 3.58 EE311 pKa = 4.37 FNANGNLFVKK321 pKa = 9.82 TSKK324 pKa = 10.21 PAFAYY329 pKa = 10.23 QGVGANTSEE338 pKa = 4.34 ANQGMFFVPPLSCEE352 pKa = 4.01 SRR354 pKa = 11.84 GKK356 pKa = 10.39 VDD358 pKa = 4.6 NIPNIEE364 pKa = 4.48 SIGNTTFTGGITIVTNVNASVNINSQPITNFATSGPFSIDD404 pKa = 2.8 IDD406 pKa = 3.75 NDD408 pKa = 4.19 GIADD412 pKa = 3.77 YY413 pKa = 8.03 EE414 pKa = 4.66 TYY416 pKa = 10.77 KK417 pKa = 9.7 VTNLTGNISVDD428 pKa = 3.42 SSDD431 pKa = 4.26 EE432 pKa = 4.18 LYY434 pKa = 10.82 CAYY437 pKa = 10.75 FNVNGAATSGSFYY450 pKa = 10.75 SGFPTAPEE458 pKa = 4.06 INFDD462 pKa = 3.93 TTVSTLGNCIPNLTLEE478 pKa = 4.46 AANTNLFDD486 pKa = 4.58 SIKK489 pKa = 9.26 WEE491 pKa = 4.32 YY492 pKa = 10.66 YY493 pKa = 10.79 NEE495 pKa = 4.25 TTSTWEE501 pKa = 3.84 EE502 pKa = 3.48 RR503 pKa = 11.84 SSNNTYY509 pKa = 10.71 KK510 pKa = 10.63 PIEE513 pKa = 4.37 SEE515 pKa = 3.69 PGKK518 pKa = 10.63 YY519 pKa = 9.73 RR520 pKa = 11.84 LIGTIDD526 pKa = 3.35 CTGATFEE533 pKa = 4.72 SIEE536 pKa = 4.36 IPVSICPDD544 pKa = 3.77 DD545 pKa = 4.31 FDD547 pKa = 6.96 GDD549 pKa = 4.63 LIIDD553 pKa = 4.18 NLDD556 pKa = 3.02 VDD558 pKa = 4.21 IDD560 pKa = 3.72 NDD562 pKa = 4.77 GILNCDD568 pKa = 3.39 EE569 pKa = 4.47 SLGNATLNLADD580 pKa = 4.8 ADD582 pKa = 3.78 NPEE585 pKa = 5.31 IIFQDD590 pKa = 3.26 SSTNSAILSRR600 pKa = 11.84 VYY602 pKa = 10.8 SEE604 pKa = 4.89 SDD606 pKa = 3.17 VTNTYY611 pKa = 9.23 TGQNNGNFQSIIYY624 pKa = 9.21 PSVDD628 pKa = 2.7 SKK630 pKa = 11.46 LQYY633 pKa = 10.44 EE634 pKa = 4.64 LNFTQNVNFKK644 pKa = 8.89 FTQNRR649 pKa = 11.84 NLDD652 pKa = 3.53 HH653 pKa = 6.94 TISDD657 pKa = 3.98 GEE659 pKa = 4.14 FFILKK664 pKa = 10.04 IGPSNKK670 pKa = 9.51 NVTLLDD676 pKa = 4.56 PDD678 pKa = 4.11 DD679 pKa = 4.01 QLLIDD684 pKa = 4.45 SNFDD688 pKa = 3.46 GEE690 pKa = 4.52 FEE692 pKa = 4.32 SGITNISASEE702 pKa = 3.48 IHH704 pKa = 6.63 FKK706 pKa = 11.17 YY707 pKa = 10.25 KK708 pKa = 10.99 ANTTGASSNFKK719 pKa = 10.26 FVANQVDD726 pKa = 3.9 QIDD729 pKa = 4.42 FKK731 pKa = 11.29 HH732 pKa = 6.07 QSTGLAASSTFSGNLEE748 pKa = 4.19 LTCFTLDD755 pKa = 3.25 SDD757 pKa = 4.36 GDD759 pKa = 4.15 GIEE762 pKa = 4.59 NMFDD766 pKa = 3.83 LDD768 pKa = 4.16 SDD770 pKa = 3.87 NDD772 pKa = 4.8 GIPDD776 pKa = 3.4 ISEE779 pKa = 3.91 SSATKK784 pKa = 10.21 IILSNTDD791 pKa = 3.0 ADD793 pKa = 5.33 LDD795 pKa = 3.92 GLDD798 pKa = 4.53 DD799 pKa = 3.93 VFIGIITNIDD809 pKa = 3.4 TDD811 pKa = 3.58 NDD813 pKa = 4.82 GIPNHH818 pKa = 6.95 LDD820 pKa = 2.75 VDD822 pKa = 4.04 ADD824 pKa = 3.52 NDD826 pKa = 4.82 GIFDD830 pKa = 3.89 FVEE833 pKa = 4.17 AGITEE838 pKa = 4.16 TDD840 pKa = 3.41 ANNDD844 pKa = 4.17 GILDD848 pKa = 4.19 DD849 pKa = 4.13 ATTANVGLNGLLNALEE865 pKa = 4.22 TSPDD869 pKa = 3.73 NNILAYY875 pKa = 9.65 TILDD879 pKa = 3.5 TDD881 pKa = 4.1 ADD883 pKa = 3.94 NIYY886 pKa = 11.24 NFLEE890 pKa = 4.31 LDD892 pKa = 3.94 ADD894 pKa = 3.83 NDD896 pKa = 3.5 NCFDD900 pKa = 3.57 VFEE903 pKa = 6.32 AGFTDD908 pKa = 4.12 NNDD911 pKa = 3.86 DD912 pKa = 4.9 GILDD916 pKa = 3.73 ATPFSVNNNGKK927 pKa = 9.94 IINNSDD933 pKa = 3.49 GYY935 pKa = 6.96 TTPNSDD941 pKa = 4.72 YY942 pKa = 9.7 ITSAPIEE949 pKa = 4.27 INTPFEE955 pKa = 4.41 DD956 pKa = 5.37 VIFCEE961 pKa = 4.06 NDD963 pKa = 2.86 TDD965 pKa = 4.84 VITIDD970 pKa = 3.3 TTADD974 pKa = 3.13 SFQWQISPDD983 pKa = 3.31 GSTWSSITDD992 pKa = 3.19 NAVYY996 pKa = 10.75 SNSTSASLQITNTPLSYY1013 pKa = 10.69 HH1014 pKa = 6.45 NYY1016 pKa = 7.99 QYY1018 pKa = 11.21 RR1019 pKa = 11.84 VILNRR1024 pKa = 11.84 TGNACTEE1031 pKa = 4.19 TSNSITLTVNPLPILKK1047 pKa = 10.33 ANPEE1051 pKa = 4.31 LNQCIEE1057 pKa = 4.15 VADD1060 pKa = 4.14 TNTTVNLTLAEE1071 pKa = 4.21 INISEE1076 pKa = 4.45 TTNVTFEE1083 pKa = 4.46 YY1084 pKa = 10.4 YY1085 pKa = 10.75 EE1086 pKa = 4.46 DD1087 pKa = 3.45 MAGANLIADD1096 pKa = 3.85 PTSYY1100 pKa = 10.29 PVQVNITEE1108 pKa = 4.25 TVYY1111 pKa = 11.2 VKK1113 pKa = 10.69 VISEE1117 pKa = 4.3 FSCEE1121 pKa = 3.89 TDD1123 pKa = 4.02 LSEE1126 pKa = 4.16 LTINVGQTIRR1136 pKa = 11.84 NSYY1139 pKa = 11.08 NDD1141 pKa = 4.28 LQPPVCDD1148 pKa = 4.84 DD1149 pKa = 3.9 FLDD1152 pKa = 3.9 VDD1154 pKa = 4.34 GNDD1157 pKa = 3.37 TAVNSDD1163 pKa = 3.38 TDD1165 pKa = 3.98 NITNFYY1171 pKa = 10.25 LDD1173 pKa = 3.22 KK1174 pKa = 10.43 TAIINGINPPMNTEE1188 pKa = 3.7 VFFYY1192 pKa = 10.61 EE1193 pKa = 4.47 NEE1195 pKa = 4.0 SDD1197 pKa = 4.37 RR1198 pKa = 11.84 DD1199 pKa = 3.6 NSLNEE1204 pKa = 3.52 IDD1206 pKa = 3.64 VTNFRR1211 pKa = 11.84 NNISKK1216 pKa = 10.81 NEE1218 pKa = 4.09 VINIPNGIQFPIFYY1232 pKa = 10.01 KK1233 pKa = 10.42 ILSTINNDD1241 pKa = 3.36 CQGIGEE1247 pKa = 4.85 FYY1249 pKa = 10.84 VQIDD1253 pKa = 4.01 TVPTAEE1259 pKa = 4.19 VVPDD1263 pKa = 5.27 LEE1265 pKa = 5.18 LCDD1268 pKa = 5.09 DD1269 pKa = 5.4 AIDD1272 pKa = 4.19 GNATNGIVQYY1282 pKa = 11.0 FDD1284 pKa = 5.42 LEE1286 pKa = 4.47 SQTTAILNGQNAADD1300 pKa = 3.87 FTVTYY1305 pKa = 10.2 HH1306 pKa = 7.98 DD1307 pKa = 4.73 SEE1309 pKa = 4.4 TDD1311 pKa = 3.15 ANAGIDD1317 pKa = 3.71 EE1318 pKa = 4.34 LTSPFEE1324 pKa = 3.78 NSVRR1328 pKa = 11.84 DD1329 pKa = 3.63 SQPIYY1334 pKa = 11.18 VRR1336 pKa = 11.84 VTNNTTGCFTDD1347 pKa = 3.2 HH1348 pKa = 6.83 TSFNVIVNPVPIANFVNDD1366 pKa = 5.67 LEE1368 pKa = 4.59 VCDD1371 pKa = 5.04 DD1372 pKa = 4.13 NSDD1375 pKa = 3.19 GSARR1379 pKa = 11.84 NGFSQSIDD1387 pKa = 3.94 LEE1389 pKa = 4.39 SQTAGILGAQDD1400 pKa = 3.75 PNIHH1404 pKa = 6.17 IVTYY1408 pKa = 10.27 HH1409 pKa = 7.04 RR1410 pKa = 11.84 SLADD1414 pKa = 3.47 AQSGNLPLVSPYY1426 pKa = 10.63 TNNTIDD1432 pKa = 3.64 RR1433 pKa = 11.84 EE1434 pKa = 4.41 TIYY1437 pKa = 11.16 VRR1439 pKa = 11.84 ILNTNSMCVNGISNFDD1455 pKa = 3.86 VIVNPEE1461 pKa = 4.09 PTFEE1465 pKa = 4.35 TPTNLAYY1472 pKa = 10.77 CDD1474 pKa = 4.77 DD1475 pKa = 5.16 DD1476 pKa = 6.7 LDD1478 pKa = 6.17 GDD1480 pKa = 4.13 DD1481 pKa = 5.54 ANGIVQNIDD1490 pKa = 3.41 LDD1492 pKa = 4.27 SKK1494 pKa = 9.78 ITEE1497 pKa = 4.09 ILGASQSPNDD1507 pKa = 3.63 FWVTFHH1513 pKa = 7.59 KK1514 pKa = 10.75 SQADD1518 pKa = 3.5 ATSGNDD1524 pKa = 3.51 PVVSPYY1530 pKa = 11.15 QNTT1533 pKa = 3.29
Molecular weight: 166.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.707
IPC2_protein 3.656
IPC_protein 3.706
Toseland 3.478
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.376
Solomon 3.706
Lehninger 3.656
Nozaki 3.808
DTASelect 4.088
Thurlkill 3.528
EMBOSS 3.656
Sillero 3.834
Patrickios 0.795
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.731
Protein with the highest isoelectric point:
>tr|A0A1B8TWH8|A0A1B8TWH8_9FLAO Malate dehydrogenase OS=Polaribacter reichenbachii OX=996801 GN=mdh PE=3 SV=1
MM1 pKa = 7.54 SVRR4 pKa = 11.84 KK5 pKa = 9.68 LKK7 pKa = 10.5 PITPGQRR14 pKa = 11.84 FRR16 pKa = 11.84 VVNGFDD22 pKa = 3.92 TITTDD27 pKa = 3.73 KK28 pKa = 10.91 PEE30 pKa = 4.37 KK31 pKa = 10.55 SLLAPKK37 pKa = 9.91 KK38 pKa = 10.38 RR39 pKa = 11.84 SGGRR43 pKa = 11.84 NNQGRR48 pKa = 11.84 MTTRR52 pKa = 11.84 NIGGGHH58 pKa = 4.99 KK59 pKa = 9.43 QRR61 pKa = 11.84 YY62 pKa = 8.74 RR63 pKa = 11.84 IIDD66 pKa = 3.84 FKK68 pKa = 10.95 RR69 pKa = 11.84 DD70 pKa = 3.68 KK71 pKa = 11.21 NDD73 pKa = 2.9 MPATVKK79 pKa = 10.14 TIEE82 pKa = 4.0 YY83 pKa = 9.86 DD84 pKa = 3.47 PNRR87 pKa = 11.84 TAFIALLSYY96 pKa = 11.49 ADD98 pKa = 3.5 GEE100 pKa = 4.22 KK101 pKa = 10.25 RR102 pKa = 11.84 YY103 pKa = 10.74 VIAQNGLKK111 pKa = 10.19 VGQTIVSGSGVSPEE125 pKa = 4.69 IGNAMPLSEE134 pKa = 4.97 IPLGTTISCIEE145 pKa = 3.88 LHH147 pKa = 6.66 PGQGAVMARR156 pKa = 11.84 SAGSFAQLMARR167 pKa = 11.84 DD168 pKa = 3.96 GKK170 pKa = 10.52 YY171 pKa = 9.12 ATVKK175 pKa = 10.42 LPSGEE180 pKa = 4.06 TRR182 pKa = 11.84 LILLTCSATIGVVSNSDD199 pKa = 3.21 HH200 pKa = 6.09 QLLVSGKK207 pKa = 8.88 AGRR210 pKa = 11.84 RR211 pKa = 11.84 RR212 pKa = 11.84 WLGRR216 pKa = 11.84 RR217 pKa = 11.84 PRR219 pKa = 11.84 VNAVRR224 pKa = 11.84 MNPVDD229 pKa = 3.4 HH230 pKa = 7.07 PMGGGEE236 pKa = 4.08 GRR238 pKa = 11.84 SSGGHH243 pKa = 4.7 PRR245 pKa = 11.84 SRR247 pKa = 11.84 NGIPAKK253 pKa = 10.41 GFKK256 pKa = 9.24 TRR258 pKa = 11.84 SKK260 pKa = 9.37 TKK262 pKa = 10.4 ASNKK266 pKa = 9.95 YY267 pKa = 9.56 IIEE270 pKa = 4.06 RR271 pKa = 11.84 RR272 pKa = 11.84 KK273 pKa = 10.1 KK274 pKa = 9.82
Molecular weight: 29.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.296
IPC2_protein 9.94
IPC_protein 10.921
Toseland 11.169
ProMoST 11.169
Dawson 11.213
Bjellqvist 11.008
Wikipedia 11.506
Rodwell 11.403
Grimsley 11.257
Solomon 11.477
Lehninger 11.418
Nozaki 11.155
DTASelect 10.994
Thurlkill 11.155
EMBOSS 11.608
Sillero 11.169
Patrickios 11.111
IPC_peptide 11.477
IPC2_peptide 10.131
IPC2.peptide.svr19 8.642
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3418
0
3418
1227233
50
2383
359.1
40.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.955 ± 0.046
0.656 ± 0.011
5.61 ± 0.029
6.479 ± 0.041
5.46 ± 0.041
6.142 ± 0.044
1.624 ± 0.018
8.363 ± 0.04
8.775 ± 0.056
9.038 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.948 ± 0.019
6.952 ± 0.051
3.13 ± 0.02
3.156 ± 0.022
3.085 ± 0.022
6.57 ± 0.033
5.857 ± 0.034
5.967 ± 0.027
1.115 ± 0.016
4.12 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here