Pacific flying fox faeces associated gemycircularvirus-8

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; Cyclovirus; unclassified Cyclovirus

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140CTP0|A0A140CTP0_9CIRC RepA OS=Pacific flying fox faeces associated gemycircularvirus-8 OX=1796000 PE=3 SV=1
MM1 pKa = 7.93PSNFHH6 pKa = 6.26LKK8 pKa = 9.01NRR10 pKa = 11.84RR11 pKa = 11.84YY12 pKa = 10.69VLFTYY17 pKa = 9.79AQSGQDD23 pKa = 3.17FDD25 pKa = 3.71YY26 pKa = 10.58WAVVDD31 pKa = 5.11LLGDD35 pKa = 4.15LGAEE39 pKa = 4.21CIIGRR44 pKa = 11.84EE45 pKa = 4.02LHH47 pKa = 6.46EE48 pKa = 5.71DD49 pKa = 3.42GGIHH53 pKa = 5.03YY54 pKa = 10.3HH55 pKa = 5.8VFADD59 pKa = 4.64FGRR62 pKa = 11.84LFSTRR67 pKa = 11.84KK68 pKa = 9.68VRR70 pKa = 11.84IFDD73 pKa = 3.47VGGKK77 pKa = 9.24HH78 pKa = 6.61PNIKK82 pKa = 10.26PIGRR86 pKa = 11.84TPAQAYY92 pKa = 9.94DD93 pKa = 3.67YY94 pKa = 10.4AIKK97 pKa = 10.96DD98 pKa = 3.52GDD100 pKa = 4.04VVAGGLGRR108 pKa = 11.84PGGDD112 pKa = 4.46CDD114 pKa = 3.84WDD116 pKa = 3.81PDD118 pKa = 4.27NFWSAATHH126 pKa = 6.46AGSADD131 pKa = 3.79EE132 pKa = 5.3FLHH135 pKa = 7.02FCDD138 pKa = 4.14QLAPRR143 pKa = 11.84DD144 pKa = 3.87LVRR147 pKa = 11.84GFPNFRR153 pKa = 11.84AYY155 pKa = 10.51ANWKK159 pKa = 7.95WNDD162 pKa = 2.99RR163 pKa = 11.84VAVYY167 pKa = 8.89DD168 pKa = 3.77QPDD171 pKa = 3.71GAVFDD176 pKa = 4.0TTAAPGIDD184 pKa = 3.11EE185 pKa = 4.73WLSQSAIGTGKK196 pKa = 10.0SRR198 pKa = 11.84EE199 pKa = 3.97RR200 pKa = 3.54

Molecular weight: 22.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140CTN9|A0A140CTN9_9CIRC Replication-associated protein OS=Pacific flying fox faeces associated gemycircularvirus-8 OX=1796000 PE=3 SV=1
MM1 pKa = 8.18PYY3 pKa = 8.98RR4 pKa = 11.84TTRR7 pKa = 11.84RR8 pKa = 11.84GRR10 pKa = 11.84RR11 pKa = 11.84SFTRR15 pKa = 11.84KK16 pKa = 7.23VTPKK20 pKa = 10.36RR21 pKa = 11.84FGGTSRR27 pKa = 11.84RR28 pKa = 11.84TFPSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84FSGKK39 pKa = 9.47RR40 pKa = 11.84RR41 pKa = 11.84FTSKK45 pKa = 10.16RR46 pKa = 11.84MTRR49 pKa = 11.84KK50 pKa = 9.66KK51 pKa = 10.79VLDD54 pKa = 3.56YY55 pKa = 10.7TSKK58 pKa = 10.73KK59 pKa = 9.58KK60 pKa = 10.26QDD62 pKa = 2.94IMLQITNIDD71 pKa = 3.53AGGVTPGPLRR81 pKa = 11.84NGPSYY86 pKa = 10.96LQANKK91 pKa = 10.03TYY93 pKa = 10.14LIPFAATARR102 pKa = 11.84PAFGANLAPGNPADD116 pKa = 4.04RR117 pKa = 11.84AIRR120 pKa = 11.84TSSEE124 pKa = 3.84CYY126 pKa = 8.72MRR128 pKa = 11.84GIKK131 pKa = 9.74EE132 pKa = 4.33RR133 pKa = 11.84ITIRR137 pKa = 11.84TDD139 pKa = 4.11SGAPWQWRR147 pKa = 11.84RR148 pKa = 11.84ICIKK152 pKa = 9.46MRR154 pKa = 11.84GSKK157 pKa = 10.37FYY159 pKa = 10.55DD160 pKa = 3.38QEE162 pKa = 4.58SGTDD166 pKa = 3.31RR167 pKa = 11.84MLSYY171 pKa = 10.92LDD173 pKa = 4.13NADD176 pKa = 3.77IPQGMARR183 pKa = 11.84VATTWTQSDD192 pKa = 4.0SAWAVLCEE200 pKa = 3.99EE201 pKa = 4.83LFAGTRR207 pKa = 11.84GYY209 pKa = 10.9DD210 pKa = 2.97WSDD213 pKa = 3.17EE214 pKa = 3.95MLAPLDD220 pKa = 3.76KK221 pKa = 11.15NRR223 pKa = 11.84VSVAYY228 pKa = 10.07DD229 pKa = 3.21YY230 pKa = 7.48TTTIRR235 pKa = 11.84SGNDD239 pKa = 2.67SGVYY243 pKa = 8.07RR244 pKa = 11.84TVNRR248 pKa = 11.84WHH250 pKa = 7.12PMNKK254 pKa = 8.14TLYY257 pKa = 10.83YY258 pKa = 10.54DD259 pKa = 4.44DD260 pKa = 3.86QDD262 pKa = 4.04KK263 pKa = 11.33GDD265 pKa = 4.06RR266 pKa = 11.84LDD268 pKa = 3.35SRR270 pKa = 11.84RR271 pKa = 11.84WSMEE275 pKa = 3.24GNRR278 pKa = 11.84GMGDD282 pKa = 3.24YY283 pKa = 11.05YY284 pKa = 10.42IFDD287 pKa = 5.18FIKK290 pKa = 11.09GNGSPDD296 pKa = 3.38DD297 pKa = 3.77QLAIEE302 pKa = 4.54YY303 pKa = 10.75NSTLYY308 pKa = 9.56WHH310 pKa = 7.05EE311 pKa = 4.08KK312 pKa = 9.32

Molecular weight: 36.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

834

200

322

278.0

31.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.393 ± 0.837

1.559 ± 0.321

9.233 ± 0.679

3.357 ± 0.338

5.276 ± 0.742

9.353 ± 0.603

2.758 ± 0.961

4.796 ± 0.106

4.556 ± 0.513

6.235 ± 0.625

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.679 ± 0.788

3.477 ± 0.361

4.676 ± 0.168

2.638 ± 0.232

8.633 ± 1.407

5.276 ± 0.897

5.516 ± 1.338

4.676 ± 0.757

3.477 ± 0.591

4.436 ± 0.449
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski