Bovine ephemeral fever virus (BEFV)
Average proteome isoelectric point is 7.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A0V8W3|A0A0A0V8W3_BEFV GDP polyribonucleotidyltransferase OS=Bovine ephemeral fever virus OX=11303 GN=L PE=4 SV=1
MM1 pKa = 7.31 SHH3 pKa = 7.47 LKK5 pKa = 9.92 PKK7 pKa = 10.41 IMTGAYY13 pKa = 9.75 DD14 pKa = 3.76 AEE16 pKa = 4.35 KK17 pKa = 10.59 LRR19 pKa = 11.84 KK20 pKa = 9.14 NLQEE24 pKa = 4.04 QIALEE29 pKa = 4.09 EE30 pKa = 4.8 DD31 pKa = 3.4 EE32 pKa = 6.26 LEE34 pKa = 4.34 NQTDD38 pKa = 3.97 FEE40 pKa = 4.85 NNKK43 pKa = 9.94 EE44 pKa = 3.97 EE45 pKa = 4.88 SNISNNPLIIKK56 pKa = 8.35 QEE58 pKa = 3.87 PGIYY62 pKa = 9.52 PIEE65 pKa = 4.63 INLEE69 pKa = 4.08 DD70 pKa = 4.0 LEE72 pKa = 4.34 KK73 pKa = 11.29 ANGIEE78 pKa = 4.02 EE79 pKa = 4.12 DD80 pKa = 3.85 WEE82 pKa = 4.14 NSLLKK87 pKa = 10.15 IIEE90 pKa = 4.46 SSDD93 pKa = 3.47 VTPKK97 pKa = 10.86 LSWDD101 pKa = 3.87 DD102 pKa = 3.58 EE103 pKa = 4.76 FEE105 pKa = 4.27 NDD107 pKa = 3.52 TYY109 pKa = 11.17 KK110 pKa = 11.06 GCNVFTKK117 pKa = 10.25 DD118 pKa = 3.82 LCNDD122 pKa = 3.38 SGNQEE127 pKa = 4.11 KK128 pKa = 11.23 NNVQIKK134 pKa = 9.72 QSSLADD140 pKa = 3.51 VAQVLSLFHH149 pKa = 6.48 IRR151 pKa = 11.84 SDD153 pKa = 3.1 VDD155 pKa = 3.55 YY156 pKa = 9.08 KK157 pKa = 10.23 TEE159 pKa = 4.01 KK160 pKa = 10.43 DD161 pKa = 3.35 NKK163 pKa = 9.64 GLVRR167 pKa = 11.84 IIKK170 pKa = 10.09 LSKK173 pKa = 9.22 KK174 pKa = 10.33 DD175 pKa = 3.8 KK176 pKa = 10.76 SIEE179 pKa = 4.19 SNKK182 pKa = 9.84 RR183 pKa = 11.84 DD184 pKa = 3.51 IVDD187 pKa = 3.55 QEE189 pKa = 4.31 SDD191 pKa = 2.9 KK192 pKa = 10.89 HH193 pKa = 5.39 TEE195 pKa = 3.99 CNSNFDD201 pKa = 3.5 AVMDD205 pKa = 3.71 QFQKK209 pKa = 11.01 GIRR212 pKa = 11.84 VKK214 pKa = 10.61 KK215 pKa = 10.24 RR216 pKa = 11.84 FGKK219 pKa = 10.76 GYY221 pKa = 10.97 VKK223 pKa = 10.44 INADD227 pKa = 3.65 NMPGTYY233 pKa = 9.53 HH234 pKa = 7.52 DD235 pKa = 5.06 LYY237 pKa = 11.47 NVISAVKK244 pKa = 9.83 GEE246 pKa = 4.14 KK247 pKa = 9.12 TVEE250 pKa = 3.53 EE251 pKa = 4.31 MIRR254 pKa = 11.84 YY255 pKa = 8.27 LFKK258 pKa = 10.55 KK259 pKa = 10.03 SKK261 pKa = 9.79 SFKK264 pKa = 10.62 SISKK268 pKa = 8.5 TLNIDD273 pKa = 3.89 EE274 pKa = 5.4 MILCC278 pKa = 4.91
Molecular weight: 32.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.046
IPC2_protein 5.054
IPC_protein 4.978
Toseland 4.863
ProMoST 5.092
Dawson 4.94
Bjellqvist 5.08
Wikipedia 4.8
Rodwell 4.838
Grimsley 4.774
Solomon 4.94
Lehninger 4.889
Nozaki 5.041
DTASelect 5.194
Thurlkill 4.851
EMBOSS 4.825
Sillero 5.118
Patrickios 4.317
IPC_peptide 4.94
IPC2_peptide 5.105
IPC2.peptide.svr19 5.11
Protein with the highest isoelectric point:
>tr|A0A0A0VBA2|A0A0A0VBA2_BEFV Isoform of A0A0A0V4V7 Alpha 3 protein OS=Bovine ephemeral fever virus OX=11303 GN=alpha PE=4 SV=1
MM1 pKa = 7.65 EE2 pKa = 6.12 KK3 pKa = 10.88 GLLSNFWNDD12 pKa = 2.85 FKK14 pKa = 11.19 QWSEE18 pKa = 3.81 DD19 pKa = 3.21 RR20 pKa = 11.84 KK21 pKa = 10.5 VEE23 pKa = 4.54 IVNWWSNLEE32 pKa = 4.17 SKK34 pKa = 10.51 VRR36 pKa = 11.84 LGFWIILIILLGILAIRR53 pKa = 11.84 IAIKK57 pKa = 10.34 VYY59 pKa = 10.12 QCVKK63 pKa = 9.78 LTTKK67 pKa = 10.14 GVKK70 pKa = 9.62 KK71 pKa = 10.09 IKK73 pKa = 10.25 RR74 pKa = 11.84 VIKK77 pKa = 10.08 KK78 pKa = 9.4 KK79 pKa = 10.62 RR80 pKa = 11.84 SIKK83 pKa = 10.22 KK84 pKa = 9.1 YY85 pKa = 10.38 RR86 pKa = 11.84 KK87 pKa = 6.76 TT88 pKa = 3.33
Molecular weight: 10.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.198
IPC2_protein 9.78
IPC_protein 9.867
Toseland 10.877
ProMoST 10.35
Dawson 10.95
Bjellqvist 10.526
Wikipedia 11.052
Rodwell 11.579
Grimsley 10.965
Solomon 10.994
Lehninger 10.979
Nozaki 10.847
DTASelect 10.526
Thurlkill 10.847
EMBOSS 11.257
Sillero 10.862
Patrickios 11.316
IPC_peptide 11.008
IPC2_peptide 9.004
IPC2.peptide.svr19 8.672
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
3
12
4809
48
2144
400.8
46.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.807 ± 0.482
1.975 ± 0.22
5.614 ± 0.369
7.798 ± 0.419
4.034 ± 0.203
5.511 ± 0.312
2.371 ± 0.227
8.526 ± 0.435
8.692 ± 0.519
10.002 ± 0.79
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.599 ± 0.343
6.758 ± 0.264
3.098 ± 0.224
2.89 ± 0.15
5.053 ± 0.324
7.216 ± 0.515
4.408 ± 0.328
4.658 ± 0.396
1.871 ± 0.209
4.055 ± 0.337
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here