Wuhan arthropod virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 5.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KKA0|A0A1L3KKA0_9VIRU Uncharacterized protein OS=Wuhan arthropod virus 1 OX=1923690 PE=4 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84CLILIFCILFACCFDD17 pKa = 4.27FNYY20 pKa = 10.04TADD23 pKa = 3.6QPSYY27 pKa = 10.3ILGGVEE33 pKa = 3.87VTDD36 pKa = 3.82CRR38 pKa = 11.84FIANCTVSYY47 pKa = 8.86QTLSQVNSSVTIVHH61 pKa = 6.89NYY63 pKa = 6.03TQFFVSSAVNVTTQVSVLIYY83 pKa = 10.48RR84 pKa = 11.84GLTDD88 pKa = 3.84GQDD91 pKa = 3.56YY92 pKa = 10.68LYY94 pKa = 10.55AGHH97 pKa = 7.48WGGEE101 pKa = 4.12NFSAVCHH108 pKa = 6.45DD109 pKa = 3.85SEE111 pKa = 6.41FYY113 pKa = 10.75ILWGNNRR120 pKa = 11.84IASYY124 pKa = 11.12KK125 pKa = 10.79SLTTDD130 pKa = 3.13NYY132 pKa = 11.33YY133 pKa = 11.38LLLKK137 pKa = 10.55LPYY140 pKa = 9.6TVDD143 pKa = 3.06VSLNPACDD151 pKa = 2.65ICFILKK157 pKa = 10.39KK158 pKa = 10.32LIQQIKK164 pKa = 8.37PYY166 pKa = 10.28IVVTLEE172 pKa = 3.82DD173 pKa = 3.73YY174 pKa = 8.83RR175 pKa = 11.84TGFRR179 pKa = 11.84YY180 pKa = 9.73PFVATSEE187 pKa = 3.9ICEE190 pKa = 4.2NSDD193 pKa = 2.74ILAPYY198 pKa = 10.38SHH200 pKa = 7.04FTLDD204 pKa = 3.39YY205 pKa = 9.87EE206 pKa = 4.29PDD208 pKa = 3.36YY209 pKa = 10.4FIPIFDD215 pKa = 4.03PEE217 pKa = 4.18IFHH220 pKa = 6.93FNPASS225 pKa = 3.18

Molecular weight: 25.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KK17|A0A1L3KK17_9VIRU Uncharacterized protein OS=Wuhan arthropod virus 1 OX=1923690 PE=4 SV=1
MM1 pKa = 6.62LTKK4 pKa = 10.14MFGLYY9 pKa = 10.63FMFGLILIVNSSVTHH24 pKa = 5.64DD25 pKa = 3.97TIIDD29 pKa = 3.61ALNYY33 pKa = 9.57NSKK36 pKa = 10.07ILDD39 pKa = 3.7SLLSLLSEE47 pKa = 4.01ITVTSTSSTANEE59 pKa = 4.38VITNNYY65 pKa = 8.93LSKK68 pKa = 10.62IQVDD72 pKa = 3.41INKK75 pKa = 9.75NYY77 pKa = 9.93HH78 pKa = 6.24LHH80 pKa = 6.48LSWDD84 pKa = 3.62EE85 pKa = 4.0TFNAIDD91 pKa = 3.7RR92 pKa = 11.84FVKK95 pKa = 10.6LGNHH99 pKa = 7.17GIALDD104 pKa = 4.84GIPGTTMSGKK114 pKa = 9.86IKK116 pKa = 10.77SNTAFIICPAMIPMSCRR133 pKa = 11.84IKK135 pKa = 9.9MRR137 pKa = 11.84HH138 pKa = 5.79AKK140 pKa = 10.09FDD142 pKa = 3.37VTRR145 pKa = 11.84THH147 pKa = 7.71DD148 pKa = 3.43YY149 pKa = 10.31TKK151 pKa = 10.65YY152 pKa = 10.71LRR154 pKa = 11.84GGTKK158 pKa = 10.59GLGKK162 pKa = 10.46

Molecular weight: 18.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

3900

147

1960

487.5

55.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.051 ± 0.474

1.949 ± 0.381

6.205 ± 0.567

5.256 ± 0.573

5.026 ± 0.472

4.615 ± 0.411

2.051 ± 0.17

8.718 ± 0.6

7.333 ± 1.159

9.256 ± 0.603

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.359 ± 0.322

6.795 ± 0.801

3.333 ± 0.488

3.308 ± 0.29

3.231 ± 0.518

7.308 ± 0.91

6.564 ± 1.309

6.718 ± 0.339

0.538 ± 0.123

4.385 ± 0.552
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski