Hubei picorna-like virus 56
Average proteome isoelectric point is 5.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KKZ0|A0A1L3KKZ0_9VIRU Uncharacterized protein OS=Hubei picorna-like virus 56 OX=1923138 PE=4 SV=1
MM1 pKa = 7.42 TSEE4 pKa = 4.31 QFTIDD9 pKa = 3.34 GDD11 pKa = 4.44 EE12 pKa = 4.16 VCGTNKK18 pKa = 9.66 KK19 pKa = 10.43 NKK21 pKa = 9.83 VITTYY26 pKa = 11.0 GIYY29 pKa = 10.52 DD30 pKa = 4.28 LFAEE34 pKa = 5.26 DD35 pKa = 4.11 EE36 pKa = 4.61 DD37 pKa = 4.21 KK38 pKa = 11.43 VSNVFHH44 pKa = 6.84 FLCLWGEE51 pKa = 4.29 GNNYY55 pKa = 9.59 FPFIKK60 pKa = 10.41 DD61 pKa = 3.33 YY62 pKa = 11.23 DD63 pKa = 4.15 NKK65 pKa = 9.91 GTTSNTVLAICSQMEE80 pKa = 4.14 DD81 pKa = 3.36 MALEE85 pKa = 5.17 LYY87 pKa = 10.52 QPTDD91 pKa = 2.65 VWGFSLLNEE100 pKa = 5.11 FYY102 pKa = 10.49 TDD104 pKa = 3.9 EE105 pKa = 4.63 LEE107 pKa = 4.23 KK108 pKa = 10.94 LCPFVIEE115 pKa = 4.32 YY116 pKa = 10.8 KK117 pKa = 10.73 NAIQSGTLDD126 pKa = 3.89 IYY128 pKa = 10.72 PDD130 pKa = 3.92 EE131 pKa = 4.57 IKK133 pKa = 10.32 ATEE136 pKa = 4.43 FYY138 pKa = 11.11 GWGTKK143 pKa = 9.37 YY144 pKa = 10.78 LFVDD148 pKa = 4.8 FRR150 pKa = 11.84 FQGLTKK156 pKa = 10.19 EE157 pKa = 4.15 KK158 pKa = 11.01 QNIFKK163 pKa = 10.8 NLTKK167 pKa = 10.75 VNFIRR172 pKa = 11.84 PGPNQIRR179 pKa = 11.84 QIINNTTEE187 pKa = 4.53 LYY189 pKa = 10.84 LPTLTRR195 pKa = 11.84 QIVINSVKK203 pKa = 10.25 QYY205 pKa = 11.18 AVNKK209 pKa = 8.85 TLFGRR214 pKa = 11.84 QSLHH218 pKa = 5.57 GPYY221 pKa = 9.9 VYY223 pKa = 10.85 EE224 pKa = 5.23 LIAGLKK230 pKa = 9.77 RR231 pKa = 11.84 EE232 pKa = 4.29 YY233 pKa = 10.63 KK234 pKa = 10.43 SGLIEE239 pKa = 4.32 LKK241 pKa = 10.6 LVNTFNGNFPIILRR255 pKa = 11.84 LDD257 pKa = 3.3 ISNKK261 pKa = 8.78 VEE263 pKa = 3.69 EE264 pKa = 4.52 TIIYY268 pKa = 10.35 AVVLDD273 pKa = 4.44 DD274 pKa = 5.91 LVPQFGTLRR283 pKa = 11.84 YY284 pKa = 8.51 PAEE287 pKa = 3.99 TLEE290 pKa = 4.48 VYY292 pKa = 10.34 SVQQVNNSLYY302 pKa = 10.61 HH303 pKa = 6.67 EE304 pKa = 4.9 PLQSSDD310 pKa = 3.85 FISNVIVANAAMAAAMIGGGLLSGLGQGFGAYY342 pKa = 8.71 GQSKK346 pKa = 10.59 ALMEE350 pKa = 4.19 MQQKK354 pKa = 8.98 QLAWLRR360 pKa = 11.84 EE361 pKa = 4.2 QQGSSQDD368 pKa = 3.5 FQRR371 pKa = 11.84 GFQQSMFDD379 pKa = 3.8 FQSLQQQRR387 pKa = 11.84 AQDD390 pKa = 3.73 FQFKK394 pKa = 8.32 QQSYY398 pKa = 10.1 GYY400 pKa = 8.84 QQQQLLQSQAFRR412 pKa = 11.84 QQDD415 pKa = 3.45 KK416 pKa = 11.03 YY417 pKa = 11.46 QLNQNQFTKK426 pKa = 10.73 EE427 pKa = 3.92 LVEE430 pKa = 4.73 SNTDD434 pKa = 2.91 ADD436 pKa = 3.49 IRR438 pKa = 11.84 KK439 pKa = 9.16 ARR441 pKa = 11.84 YY442 pKa = 7.83 AQQLAGYY449 pKa = 7.87 RR450 pKa = 11.84 TDD452 pKa = 3.27 GAVYY456 pKa = 10.24 GLSQGTGRR464 pKa = 11.84 GGLGHH469 pKa = 7.71 PDD471 pKa = 3.28 APIGATLMPRR481 pKa = 11.84 SVSTQTNSPNVYY493 pKa = 8.43 LTKK496 pKa = 9.55 PTGSYY501 pKa = 9.51 VGPMSNRR508 pKa = 11.84 MDD510 pKa = 3.87 FF511 pKa = 3.54
Molecular weight: 57.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.087
IPC2_protein 5.258
IPC_protein 5.194
Toseland 5.118
ProMoST 5.321
Dawson 5.169
Bjellqvist 5.308
Wikipedia 5.041
Rodwell 5.08
Grimsley 5.041
Solomon 5.169
Lehninger 5.13
Nozaki 5.296
DTASelect 5.448
Thurlkill 5.118
EMBOSS 5.08
Sillero 5.359
Patrickios 3.745
IPC_peptide 5.181
IPC2_peptide 5.359
IPC2.peptide.svr19 5.365
Protein with the highest isoelectric point:
>tr|A0A1L3KKZ0|A0A1L3KKZ0_9VIRU Uncharacterized protein OS=Hubei picorna-like virus 56 OX=1923138 PE=4 SV=1
MM1 pKa = 7.71 SDD3 pKa = 2.97 SGEE6 pKa = 4.14 NFSKK10 pKa = 10.86 EE11 pKa = 3.84 FLTTCEE17 pKa = 3.78 GLYY20 pKa = 10.66 DD21 pKa = 3.54 ITRR24 pKa = 11.84 EE25 pKa = 4.6 SLNLWFNQLFTNALDD40 pKa = 3.81 PHH42 pKa = 6.23 STMSWISNIVAGCSSLILSIVTLFSSSSGVIMKK75 pKa = 10.23 ILGGANLLANFHH87 pKa = 6.6 SLLITIKK94 pKa = 10.29 QVNDD98 pKa = 3.39 SEE100 pKa = 4.53 FDD102 pKa = 3.44 LDD104 pKa = 4.0 KK105 pKa = 11.55 ALFKK109 pKa = 10.85 AKK111 pKa = 10.05 HH112 pKa = 6.52 DD113 pKa = 3.98 LTEE116 pKa = 4.22 HH117 pKa = 6.55 FPTEE121 pKa = 3.97 EE122 pKa = 3.71 EE123 pKa = 4.24 KK124 pKa = 10.38 IYY126 pKa = 11.26 ANAGFDD132 pKa = 5.19 IGGDD136 pKa = 3.23 WLKK139 pKa = 11.01 AISFGLTTLFTAIVAGFGYY158 pKa = 8.04 STKK161 pKa = 10.16 GLEE164 pKa = 4.93 GITSLFRR171 pKa = 11.84 TLNFTNAGFSTLKK184 pKa = 10.22 TGIDD188 pKa = 3.48 KK189 pKa = 10.18 MLSTLICEE197 pKa = 4.83 KK198 pKa = 10.67 YY199 pKa = 10.32 DD200 pKa = 3.52 PLYY203 pKa = 10.77 EE204 pKa = 4.01 EE205 pKa = 5.07 RR206 pKa = 11.84 KK207 pKa = 9.87 VLTEE211 pKa = 3.65 LVEE214 pKa = 4.58 RR215 pKa = 11.84 SQEE218 pKa = 4.01 MVRR221 pKa = 11.84 TPIANISVCNKK232 pKa = 9.46 KK233 pKa = 10.26 RR234 pKa = 11.84 LALKK238 pKa = 10.74 DD239 pKa = 3.91 NIHH242 pKa = 7.08 EE243 pKa = 4.38 INVALVQYY251 pKa = 9.56 KK252 pKa = 9.85 HH253 pKa = 6.45 QDD255 pKa = 3.14 TGPLRR260 pKa = 11.84 TVLIGLVKK268 pKa = 10.22 EE269 pKa = 4.19 LNKK272 pKa = 10.39 KK273 pKa = 10.37 LEE275 pKa = 4.75 EE276 pKa = 4.42 IEE278 pKa = 4.38 HH279 pKa = 6.28 SPDD282 pKa = 2.61 IRR284 pKa = 11.84 QVTTGIFLQGKK295 pKa = 9.11 RR296 pKa = 11.84 GIGKK300 pKa = 8.35 STFVNYY306 pKa = 9.68 LAKK309 pKa = 10.43 QLAKK313 pKa = 10.66 EE314 pKa = 4.04 FDD316 pKa = 3.65 WNDD319 pKa = 3.38 RR320 pKa = 11.84 IYY322 pKa = 11.09 NVSFGVRR329 pKa = 11.84 FHH331 pKa = 6.7 EE332 pKa = 4.81 PYY334 pKa = 9.88 MNEE337 pKa = 4.97 DD338 pKa = 2.9 ILIYY342 pKa = 11.0 NEE344 pKa = 4.16 FCSTIDD350 pKa = 3.7 PDD352 pKa = 4.2 PFLAEE357 pKa = 4.08 ANKK360 pKa = 9.93 ICSSDD365 pKa = 3.2 PHH367 pKa = 7.59 NFEE370 pKa = 4.44 SASLSGKK377 pKa = 9.84 VSNCQGKK384 pKa = 9.07 VLVVTTNSMEE394 pKa = 3.9 ADD396 pKa = 3.03 IPEE399 pKa = 4.33 FRR401 pKa = 11.84 PHH403 pKa = 6.51 AVGAFWDD410 pKa = 3.7 RR411 pKa = 11.84 FIILEE416 pKa = 4.1 VLDD419 pKa = 4.16 PLISGRR425 pKa = 11.84 RR426 pKa = 11.84 KK427 pKa = 9.68 IVDD430 pKa = 2.91 HH431 pKa = 6.79 RR432 pKa = 11.84 KK433 pKa = 9.33 PDD435 pKa = 4.02 FSHH438 pKa = 6.62 LRR440 pKa = 11.84 FNIKK444 pKa = 9.36 EE445 pKa = 4.18 VQDD448 pKa = 4.78 LEE450 pKa = 4.32 NQSPSFTTLFKK461 pKa = 10.78 DD462 pKa = 3.54 VSVFDD467 pKa = 3.64 VMTLIKK473 pKa = 9.91 TKK475 pKa = 10.26 ISYY478 pKa = 9.87 EE479 pKa = 3.69 IIKK482 pKa = 9.97 FHH484 pKa = 6.03 EE485 pKa = 4.56 AKK487 pKa = 10.4 QIEE490 pKa = 4.43 LSEE493 pKa = 4.25 HH494 pKa = 5.82 NKK496 pKa = 10.55 NIINQYY502 pKa = 9.48 VPPTEE507 pKa = 3.77 GTIPFKK513 pKa = 10.89 YY514 pKa = 10.11 FGMEE518 pKa = 5.5 AIIQPNAHH526 pKa = 6.31 SRR528 pKa = 11.84 DD529 pKa = 3.68 FLILRR534 pKa = 11.84 LQGKK538 pKa = 9.11 EE539 pKa = 4.03 GTGKK543 pKa = 7.18 TTLANSIAEE552 pKa = 4.04 RR553 pKa = 11.84 WIKK556 pKa = 9.73 LTTDD560 pKa = 3.27 AEE562 pKa = 4.51 GQSTFKK568 pKa = 10.52 TVLVTNLRR576 pKa = 11.84 DD577 pKa = 3.72 FRR579 pKa = 11.84 AVSEE583 pKa = 4.09 PAVYY587 pKa = 10.35 ILDD590 pKa = 4.09 DD591 pKa = 4.76 CISSKK596 pKa = 10.33 MNYY599 pKa = 8.35 IASHH603 pKa = 6.16 KK604 pKa = 10.25 FVEE607 pKa = 4.95 TMNSTHH613 pKa = 6.81 KK614 pKa = 10.69 SSLFIITSNEE624 pKa = 3.79 SLKK627 pKa = 10.74 EE628 pKa = 3.96 LEE630 pKa = 3.96 FSKK633 pKa = 11.19 NKK635 pKa = 8.95 WNRR638 pKa = 11.84 LLSLIKK644 pKa = 8.97 WTDD647 pKa = 2.91 CSLAYY652 pKa = 10.42 NLSEE656 pKa = 3.9 YY657 pKa = 11.01 DD658 pKa = 3.36 HH659 pKa = 7.21 LYY661 pKa = 10.73 SGIARR666 pKa = 11.84 RR667 pKa = 11.84 IGCDD671 pKa = 2.51 SVYY674 pKa = 10.65 YY675 pKa = 10.72 SKK677 pKa = 11.48 GQINNTLAVKK687 pKa = 10.17 HH688 pKa = 6.48 CILIDD693 pKa = 3.83 FPGEE697 pKa = 3.84 YY698 pKa = 8.37 PTFKK702 pKa = 10.86 KK703 pKa = 10.69 NIITEE708 pKa = 3.97 NQIFQKK714 pKa = 10.96 LVDD717 pKa = 3.83 TYY719 pKa = 11.46 VGYY722 pKa = 10.62 KK723 pKa = 9.9 KK724 pKa = 10.28 EE725 pKa = 3.9 IYY727 pKa = 10.56 NIVILNKK734 pKa = 10.27 RR735 pKa = 11.84 PDD737 pKa = 3.66 SEE739 pKa = 4.69 LEE741 pKa = 4.03 SVDD744 pKa = 5.63 FEE746 pKa = 4.4 LHH748 pKa = 6.08 CEE750 pKa = 4.03 DD751 pKa = 6.01 RR752 pKa = 11.84 EE753 pKa = 4.34 TLLSKK758 pKa = 10.22 LRR760 pKa = 11.84 KK761 pKa = 7.3 RR762 pKa = 11.84 TEE764 pKa = 4.0 VINMYY769 pKa = 10.27 LRR771 pKa = 11.84 PNEE774 pKa = 4.21 EE775 pKa = 3.45 IGLQVSDD782 pKa = 4.28 RR783 pKa = 11.84 VLDD786 pKa = 3.74 QLQQNHH792 pKa = 6.06 GGIINPSDD800 pKa = 4.36 FICKK804 pKa = 9.49 EE805 pKa = 3.97 VFQEE809 pKa = 5.03 NIPPDD814 pKa = 3.63 EE815 pKa = 4.76 KK816 pKa = 11.15 YY817 pKa = 10.38 IAIATRR823 pKa = 11.84 MSKK826 pKa = 10.29 VLLKK830 pKa = 9.91 YY831 pKa = 10.69 FPDD834 pKa = 3.49 PTFRR838 pKa = 11.84 IYY840 pKa = 10.21 IKK842 pKa = 9.7 KK843 pKa = 7.82 TRR845 pKa = 11.84 QTAYY849 pKa = 9.28 FHH851 pKa = 7.01 NGFLYY856 pKa = 10.47 YY857 pKa = 10.7 YY858 pKa = 9.08 YY859 pKa = 10.64 DD860 pKa = 3.78 HH861 pKa = 7.4 PLEE864 pKa = 4.14 VLRR867 pKa = 11.84 VEE869 pKa = 5.4 DD870 pKa = 3.48 NLLRR874 pKa = 11.84 INLSKK879 pKa = 10.85 VKK881 pKa = 10.15 YY882 pKa = 10.44 VEE884 pKa = 5.09 AIDD887 pKa = 3.62 IARR890 pKa = 11.84 SLVLTSPDD898 pKa = 3.17 EE899 pKa = 4.27 KK900 pKa = 11.14 FKK902 pKa = 10.59 GTASSLSIMEE912 pKa = 4.2 YY913 pKa = 9.23 EE914 pKa = 4.48 TIKK917 pKa = 10.48 QYY919 pKa = 11.3 YY920 pKa = 8.75 YY921 pKa = 11.27 SSLKK925 pKa = 10.22 KK926 pKa = 10.44 KK927 pKa = 9.48 EE928 pKa = 4.0 VNHH931 pKa = 7.01 FIIDD935 pKa = 3.7 VTKK938 pKa = 10.56 HH939 pKa = 4.96 IEE941 pKa = 4.19 GFKK944 pKa = 10.83 KK945 pKa = 8.78 EE946 pKa = 4.42 TNPKK950 pKa = 9.6 LLKK953 pKa = 10.11 NLLRR957 pKa = 11.84 ARR959 pKa = 11.84 SNKK962 pKa = 9.28 VLIFAVTGLVGISSAGILYY981 pKa = 8.86 TLYY984 pKa = 10.83 KK985 pKa = 9.87 IFKK988 pKa = 9.73 SKK990 pKa = 10.28 YY991 pKa = 9.69 DD992 pKa = 3.64 KK993 pKa = 11.43 VNSNAFANIGSTPPTRR1009 pKa = 11.84 TSASQNTHH1017 pKa = 6.9 DD1018 pKa = 5.81 LLDD1021 pKa = 3.72 TKK1023 pKa = 10.47 ILRR1026 pKa = 11.84 EE1027 pKa = 3.86 KK1028 pKa = 10.83 DD1029 pKa = 3.33 LLASRR1034 pKa = 11.84 QFPPRR1039 pKa = 11.84 EE1040 pKa = 3.84 VNINALVPMSDD1051 pKa = 3.77 YY1052 pKa = 11.3 SWVRR1056 pKa = 11.84 KK1057 pKa = 9.46 GKK1059 pKa = 10.04 GYY1061 pKa = 11.12 SNIYY1065 pKa = 9.22 PNNLGLEE1072 pKa = 4.25 LEE1074 pKa = 4.34 HH1075 pKa = 8.4 DD1076 pKa = 5.15 GINTLIKK1083 pKa = 10.11 KK1084 pKa = 10.1 DD1085 pKa = 3.71 LAKK1088 pKa = 10.45 KK1089 pKa = 9.03 PHH1091 pKa = 5.36 EE1092 pKa = 4.38 HH1093 pKa = 5.88 VCIKK1097 pKa = 10.48 CGCLYY1102 pKa = 11.05 SHH1104 pKa = 6.42 IHH1106 pKa = 6.22 KK1107 pKa = 10.34 FNPSYY1112 pKa = 9.86 THH1114 pKa = 5.06 TQYY1117 pKa = 10.99 PGQCPNSSCEE1127 pKa = 4.03 YY1128 pKa = 9.52 YY1129 pKa = 10.96 HH1130 pKa = 7.62 NGDD1133 pKa = 3.53 NPKK1136 pKa = 10.48 NAILIQKK1143 pKa = 8.62 DD1144 pKa = 3.63 YY1145 pKa = 11.71 HH1146 pKa = 4.66 MTEE1149 pKa = 3.9 YY1150 pKa = 11.04 HH1151 pKa = 6.96 LFTPNALSKK1160 pKa = 11.13 CMGPEE1165 pKa = 3.81 FEE1167 pKa = 5.62 IVTEE1171 pKa = 3.94 SLKK1174 pKa = 10.88 RR1175 pKa = 11.84 FFSALSQEE1183 pKa = 4.38 QRR1185 pKa = 11.84 RR1186 pKa = 11.84 EE1187 pKa = 3.81 VYY1189 pKa = 10.38 DD1190 pKa = 3.81 QIRR1193 pKa = 11.84 QCKK1196 pKa = 9.84 FEE1198 pKa = 5.62 GDD1200 pKa = 3.72 VAFKK1204 pKa = 10.84 KK1205 pKa = 10.48 LIKK1208 pKa = 10.01 RR1209 pKa = 11.84 YY1210 pKa = 9.46 PEE1212 pKa = 3.59 IKK1214 pKa = 9.79 EE1215 pKa = 4.14 CVDD1218 pKa = 3.01 IAMHH1222 pKa = 6.92 ANMLTDD1228 pKa = 3.66 SDD1230 pKa = 4.63 MIEE1233 pKa = 4.55 CDD1235 pKa = 3.41 LSEE1238 pKa = 4.93 VEE1240 pKa = 4.66 ILHH1243 pKa = 6.6 KK1244 pKa = 10.51 KK1245 pKa = 10.22 LNRR1248 pKa = 11.84 CYY1250 pKa = 11.05 VRR1252 pKa = 11.84 LTKK1255 pKa = 10.39 DD1256 pKa = 3.08 LYY1258 pKa = 11.0 KK1259 pKa = 10.71 SYY1261 pKa = 11.13 ALHH1264 pKa = 6.77 IKK1266 pKa = 10.1 DD1267 pKa = 4.2 GYY1269 pKa = 9.52 FISVAHH1275 pKa = 6.59 CFDD1278 pKa = 3.46 KK1279 pKa = 11.8 VEE1281 pKa = 4.35 DD1282 pKa = 3.91 QLILSDD1288 pKa = 3.38 NGNRR1292 pKa = 11.84 FAAVVVKK1299 pKa = 10.39 ILRR1302 pKa = 11.84 EE1303 pKa = 3.5 RR1304 pKa = 11.84 DD1305 pKa = 3.21 LAVIRR1310 pKa = 11.84 CDD1312 pKa = 4.42 LLKK1315 pKa = 10.79 ALPKK1319 pKa = 9.88 SLQYY1323 pKa = 10.16 FASSHH1328 pKa = 5.76 EE1329 pKa = 4.06 EE1330 pKa = 3.79 MPLYY1334 pKa = 10.7 AHH1336 pKa = 7.0 FMRR1339 pKa = 11.84 CGPTMSLLTGGLEE1352 pKa = 3.85 YY1353 pKa = 10.72 KK1354 pKa = 10.23 EE1355 pKa = 3.94 YY1356 pKa = 10.94 QEE1358 pKa = 5.89 FIGKK1362 pKa = 8.94 VCDD1365 pKa = 3.37 NEE1367 pKa = 4.34 YY1368 pKa = 10.51 YY1369 pKa = 10.5 KK1370 pKa = 10.86 PSTEE1374 pKa = 3.68 ILKK1377 pKa = 10.26 LRR1379 pKa = 11.84 RR1380 pKa = 11.84 AGLRR1384 pKa = 11.84 VLDD1387 pKa = 3.98 VVSKK1391 pKa = 10.61 GDD1393 pKa = 3.6 CGFPFIGKK1401 pKa = 8.8 IRR1403 pKa = 11.84 GSLRR1407 pKa = 11.84 ILGIHH1412 pKa = 6.06 NAYY1415 pKa = 9.63 INEE1418 pKa = 3.95 GVIFGSFISSQLLKK1432 pKa = 10.46 EE1433 pKa = 4.34 LLSSPFPNALALSTPVVLKK1452 pKa = 10.69 IPIVKK1457 pKa = 10.01 EE1458 pKa = 3.91 DD1459 pKa = 3.82 MSPSTLEE1466 pKa = 3.22 MSMPIEE1472 pKa = 4.15 YY1473 pKa = 10.66 ADD1475 pKa = 3.49 VFDD1478 pKa = 6.36 RR1479 pKa = 11.84 SIKK1482 pKa = 9.68 PDD1484 pKa = 2.97 PRR1486 pKa = 11.84 MPVDD1490 pKa = 4.36 SPLKK1494 pKa = 9.96 SIGYY1498 pKa = 7.99 HH1499 pKa = 5.4 TIFANPSKK1507 pKa = 10.66 LKK1509 pKa = 10.07 EE1510 pKa = 3.78 KK1511 pKa = 10.74 HH1512 pKa = 5.41 KK1513 pKa = 9.48 THH1515 pKa = 6.95 DD1516 pKa = 4.3 LEE1518 pKa = 6.42 LITPNLQLPAAFNLNYY1534 pKa = 9.36 VTDD1537 pKa = 4.42 FSDD1540 pKa = 3.07 IVKK1543 pKa = 10.57 NGFGRR1548 pKa = 11.84 YY1549 pKa = 9.37 DD1550 pKa = 3.55 PLLTQAFKK1558 pKa = 11.46 YY1559 pKa = 10.47 KK1560 pKa = 9.49 FTNQPIDD1567 pKa = 3.42 FDD1569 pKa = 4.23 AYY1571 pKa = 9.92 KK1572 pKa = 10.44 LAEE1575 pKa = 4.57 QIVQDD1580 pKa = 4.23 YY1581 pKa = 11.15 NLLTYY1586 pKa = 10.52 CSEE1589 pKa = 4.1 GPLRR1593 pKa = 11.84 IITEE1597 pKa = 4.11 YY1598 pKa = 10.81 EE1599 pKa = 4.14 AINGMRR1605 pKa = 11.84 RR1606 pKa = 11.84 PFLANFDD1613 pKa = 3.89 ITTSAGPYY1621 pKa = 9.99 AKK1623 pKa = 10.46 YY1624 pKa = 10.34 FGNAYY1629 pKa = 9.78 FKK1631 pKa = 11.04 KK1632 pKa = 10.52 DD1633 pKa = 3.12 IFNVTMHH1640 pKa = 6.68 NGVPEE1645 pKa = 4.07 YY1646 pKa = 10.06 TFNSSPIAQSIRR1658 pKa = 11.84 NNMKK1662 pKa = 8.98 QQKK1665 pKa = 9.79 KK1666 pKa = 10.25 LLFNKK1671 pKa = 9.78 HH1672 pKa = 6.06 IPPCLISQDD1681 pKa = 3.57 NAKK1684 pKa = 10.43 VEE1686 pKa = 4.32 MIEE1689 pKa = 4.72 KK1690 pKa = 10.22 EE1691 pKa = 3.94 KK1692 pKa = 10.93 ALKK1695 pKa = 10.69 GKK1697 pKa = 9.82 VRR1699 pKa = 11.84 LFNNIDD1705 pKa = 3.38 PSINAILKK1713 pKa = 8.74 MLFGHH1718 pKa = 7.29 WFAQTMTKK1726 pKa = 10.35 SADD1729 pKa = 2.99 GYY1731 pKa = 10.13 YY1732 pKa = 10.53 AIGQNPYY1739 pKa = 7.74 TTSTEE1744 pKa = 3.13 IWHH1747 pKa = 6.61 RR1748 pKa = 11.84 FSTKK1752 pKa = 10.02 DD1753 pKa = 3.43 GKK1755 pKa = 10.47 ILNTDD1760 pKa = 4.01 FRR1762 pKa = 11.84 AFDD1765 pKa = 3.54 KK1766 pKa = 10.53 TLHH1769 pKa = 6.12 PRR1771 pKa = 11.84 LIKK1774 pKa = 10.33 SFCKK1778 pKa = 10.03 IACSLMKK1785 pKa = 10.47 VKK1787 pKa = 10.48 PKK1789 pKa = 10.25 YY1790 pKa = 9.76 NKK1792 pKa = 8.81 EE1793 pKa = 4.1 AIYY1796 pKa = 10.09 EE1797 pKa = 4.56 AISKK1801 pKa = 7.77 TLIHH1805 pKa = 6.68 AVHH1808 pKa = 6.73 LLDD1811 pKa = 4.0 GSIYY1815 pKa = 10.56 VVNNGNEE1822 pKa = 3.97 SGTFVTTLLNCVCAHH1837 pKa = 7.38 IIFTYY1842 pKa = 10.0 TFIKK1846 pKa = 9.5 CWQRR1850 pKa = 11.84 IPKK1853 pKa = 9.1 YY1854 pKa = 10.42 INIKK1858 pKa = 8.75 VTLKK1862 pKa = 10.89 SIMQYY1867 pKa = 10.68 SEE1869 pKa = 4.14 LAILGDD1875 pKa = 3.47 DD1876 pKa = 3.73 TTRR1879 pKa = 11.84 KK1880 pKa = 9.51 VSSEE1884 pKa = 3.87 IPITEE1889 pKa = 3.54 QDD1891 pKa = 3.36 LFHH1894 pKa = 6.36 YY1895 pKa = 10.05 AKK1897 pKa = 10.57 EE1898 pKa = 4.22 LGTEE1902 pKa = 4.0 CTKK1905 pKa = 11.01 AKK1907 pKa = 10.5 SGEE1910 pKa = 4.12 DD1911 pKa = 3.12 TDD1913 pKa = 5.96 NIDD1916 pKa = 3.21 FCSRR1920 pKa = 11.84 TFYY1923 pKa = 10.26 WCDD1926 pKa = 3.05 KK1927 pKa = 10.52 DD1928 pKa = 4.07 QIVYY1932 pKa = 9.46 PRR1934 pKa = 11.84 LKK1936 pKa = 9.29 QCSIFGLLRR1945 pKa = 11.84 WFAEE1949 pKa = 4.24 FSKK1952 pKa = 11.06 NQIRR1956 pKa = 11.84 NNLMVALFEE1965 pKa = 4.65 ASMYY1969 pKa = 10.31 DD1970 pKa = 2.78 IKK1972 pKa = 10.85 FYY1974 pKa = 11.11 QDD1976 pKa = 3.26 VLQDD1980 pKa = 3.82 SMTVCNHH1987 pKa = 5.98 YY1988 pKa = 10.88 DD1989 pKa = 3.07 VDD1991 pKa = 3.43 IRR1993 pKa = 11.84 TVPFTNYY2000 pKa = 9.08 EE2001 pKa = 3.88 MARR2004 pKa = 11.84 ARR2006 pKa = 11.84 LKK2008 pKa = 11.3 SMLLNDD2014 pKa = 5.34 CEE2016 pKa = 4.43 YY2017 pKa = 11.39 QDD2019 pKa = 4.14 LTALANRR2026 pKa = 11.84 NQIFEE2031 pKa = 4.33 SDD2033 pKa = 2.94 IHH2035 pKa = 6.88 NITVILKK2042 pKa = 10.18 QNLLNTASAISANPEE2057 pKa = 3.93 RR2058 pKa = 11.84 IEE2060 pKa = 5.35 SIVNQHH2066 pKa = 6.39 KK2067 pKa = 10.83 ADD2069 pKa = 3.77 NKK2071 pKa = 9.2 TSKK2074 pKa = 10.8 ALQKK2078 pKa = 9.66 WALALNEE2085 pKa = 4.39 DD2086 pKa = 4.58 KK2087 pKa = 10.63 MVLGNPTNNPVSDD2100 pKa = 4.07 CLEE2103 pKa = 4.98 LISKK2107 pKa = 10.23 LKK2109 pKa = 9.49 TEE2111 pKa = 4.65 KK2112 pKa = 9.21 PTEE2115 pKa = 4.39 RR2116 pKa = 11.84 YY2117 pKa = 8.14 TSEE2120 pKa = 4.5 GPSNSPTHH2128 pKa = 6.29 SCTITYY2134 pKa = 9.53 NRR2136 pKa = 11.84 KK2137 pKa = 8.64 QFGGEE2142 pKa = 4.32 GPSKK2146 pKa = 10.9 ASAKK2150 pKa = 8.67 NQAYY2154 pKa = 9.11 GALRR2158 pKa = 11.84 EE2159 pKa = 4.0 YY2160 pKa = 11.02 LRR2162 pKa = 11.84 DD2163 pKa = 3.95 NIVVNAICEE2172 pKa = 4.15 DD2173 pKa = 3.69 QASKK2177 pKa = 10.76 SYY2179 pKa = 10.66 EE2180 pKa = 4.13 AIKK2183 pKa = 10.49 KK2184 pKa = 8.41 VAIRR2188 pKa = 11.84 ATALLIEE2195 pKa = 4.27 HH2196 pKa = 7.31 HH2197 pKa = 6.67 ISQAKK2202 pKa = 8.09 ANSMGRR2208 pKa = 11.84 PSVILVSKK2216 pKa = 10.48 RR2217 pKa = 11.84 SYY2219 pKa = 11.06 NGDD2222 pKa = 2.79 ISKK2225 pKa = 10.15 IDD2227 pKa = 3.37 GFDD2230 pKa = 3.2 RR2231 pKa = 11.84 FLLDD2235 pKa = 2.9 GHH2237 pKa = 6.85 CYY2239 pKa = 9.48 FLSPIIQEE2247 pKa = 4.37 LGRR2250 pKa = 11.84 TQAASCYY2257 pKa = 9.74 LRR2259 pKa = 11.84 YY2260 pKa = 9.59 PGITMNSDD2268 pKa = 2.99 KK2269 pKa = 10.75 EE2270 pKa = 4.24 IIMDD2274 pKa = 4.22 LRR2276 pKa = 11.84 NEE2278 pKa = 3.86 HH2279 pKa = 6.66 RR2280 pKa = 11.84 SYY2282 pKa = 11.47 SLRR2285 pKa = 11.84 NVEE2288 pKa = 4.23 EE2289 pKa = 4.01 EE2290 pKa = 4.16 KK2291 pKa = 10.88 DD2292 pKa = 3.76 YY2293 pKa = 11.69 DD2294 pKa = 4.74 DD2295 pKa = 3.95 EE2296 pKa = 4.84 TEE2298 pKa = 4.91 LIYY2301 pKa = 11.26 ANATKK2306 pKa = 10.71 EE2307 pKa = 4.14 EE2308 pKa = 4.57 GEE2310 pKa = 4.39 SLGGVLNDD2318 pKa = 3.89 IPPAASTNILLDD2330 pKa = 3.94 SEE2332 pKa = 5.2 NSLHH2336 pKa = 6.2 MPIVMNYY2343 pKa = 9.67 SGPSNALMNAGGINFNIMDD2362 pKa = 4.66 LVYY2365 pKa = 9.8 GQFVASEE2372 pKa = 4.26 YY2373 pKa = 11.15 KK2374 pKa = 10.61 LDD2376 pKa = 3.64 VDD2378 pKa = 3.88 GTKK2381 pKa = 10.81 DD2382 pKa = 3.16 RR2383 pKa = 11.84 GQVLAVVPYY2392 pKa = 10.83 DD2393 pKa = 4.2 PINNSFINDD2402 pKa = 3.68 YY2403 pKa = 10.55 IKK2405 pKa = 10.72 NYY2407 pKa = 10.22 CYY2409 pKa = 10.65 LHH2411 pKa = 6.7 DD2412 pKa = 4.31 RR2413 pKa = 11.84 CAGSWLLCVSSAGNKK2428 pKa = 10.19 SMIGSLGFSWQPTIWKK2444 pKa = 7.28 GTTIAMAEE2452 pKa = 3.87 ATKK2455 pKa = 11.0 YY2456 pKa = 11.09 SFFTTSVSEE2465 pKa = 4.34 SFSYY2469 pKa = 9.61 TIAMTDD2475 pKa = 3.02 ARR2477 pKa = 11.84 QSEE2480 pKa = 5.24 FYY2482 pKa = 9.08 RR2483 pKa = 11.84 TLVRR2487 pKa = 11.84 DD2488 pKa = 4.04 SKK2490 pKa = 11.19 GKK2492 pKa = 10.5 SSMTEE2497 pKa = 3.74 TPSIVIFVHH2506 pKa = 6.26 TKK2508 pKa = 7.75 SQPVFTEE2515 pKa = 3.88 KK2516 pKa = 10.65 RR2517 pKa = 11.84 ATTVNFYY2524 pKa = 11.29 SKK2526 pKa = 10.51 LASNVDD2532 pKa = 3.44 AAKK2535 pKa = 10.76 NPAVKK2540 pKa = 10.04 PFIASNPMLAIKK2552 pKa = 8.34 QTSEE2556 pKa = 3.62 TDD2558 pKa = 2.89 KK2559 pKa = 11.35 TSILNFF2565 pKa = 3.76
Molecular weight: 291.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.726
IPC2_protein 7.044
IPC_protein 6.883
Toseland 6.81
ProMoST 7.468
Dawson 7.541
Bjellqvist 7.892
Wikipedia 7.468
Rodwell 7.556
Grimsley 6.912
Solomon 7.541
Lehninger 7.571
Nozaki 8.083
DTASelect 7.746
Thurlkill 7.805
EMBOSS 7.805
Sillero 8.039
Patrickios 4.139
IPC_peptide 7.541
IPC2_peptide 7.117
IPC2.peptide.svr19 7.051
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
3076
511
2565
1538.0
174.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.624 ± 0.157
1.723 ± 0.433
5.397 ± 0.142
6.014 ± 0.337
5.137 ± 0.339
5.137 ± 1.241
2.243 ± 0.673
7.672 ± 1.011
7.445 ± 0.996
9.33 ± 0.029
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.081 ± 0.033
6.112 ± 0.021
3.934 ± 0.19
4.031 ± 2.562
3.901 ± 0.175
7.542 ± 0.771
6.112 ± 0.16
5.137 ± 0.158
0.715 ± 0.031
4.714 ± 0.353
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here