Sodalis-like symbiont of Philaenus spumarius
Average proteome isoelectric point is 7.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1600 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A261QLW3|A0A261QLW3_9GAMM FAD-dependent 2-octaprenylphenol hydroxylase OS=Sodalis-like symbiont of Philaenus spumarius OX=1189304 GN=CE195_12850 PE=3 SV=1
MM1 pKa = 7.34 AHH3 pKa = 6.58 SFLDD7 pKa = 3.81 EE8 pKa = 3.95 QHH10 pKa = 6.65 EE11 pKa = 4.36 EE12 pKa = 3.64 TRR14 pKa = 11.84 LIIDD18 pKa = 3.94 EE19 pKa = 4.4 LLEE22 pKa = 5.14 DD23 pKa = 4.52 GSDD26 pKa = 3.56 PEE28 pKa = 3.94 ALYY31 pKa = 9.94 TIEE34 pKa = 4.44 HH35 pKa = 6.77 HH36 pKa = 7.21 LSADD40 pKa = 4.33 DD41 pKa = 4.55 FDD43 pKa = 4.5 TLEE46 pKa = 4.28 KK47 pKa = 10.7 LAVEE51 pKa = 4.3 AFKK54 pKa = 10.95 LGYY57 pKa = 9.79 EE58 pKa = 4.19 VTDD61 pKa = 3.75 AEE63 pKa = 4.26 EE64 pKa = 4.96 LEE66 pKa = 4.45 VEE68 pKa = 4.3 GGEE71 pKa = 5.58 KK72 pKa = 10.49 LMCCDD77 pKa = 3.75 AVSEE81 pKa = 4.25 IALQAEE87 pKa = 4.82 LIDD90 pKa = 4.04 AQVEE94 pKa = 4.25 QLFTLTLRR102 pKa = 11.84 LGVNYY107 pKa = 9.91 DD108 pKa = 2.63 GWGTYY113 pKa = 10.12 FEE115 pKa = 5.85 DD116 pKa = 4.77 PDD118 pKa = 4.88 GEE120 pKa = 4.84 EE121 pKa = 4.34 FDD123 pKa = 5.31 QDD125 pKa = 5.28 DD126 pKa = 4.17 EE127 pKa = 5.47 DD128 pKa = 5.83 GPADD132 pKa = 3.97 SDD134 pKa = 3.47 EE135 pKa = 4.21 MHH137 pKa = 6.81 ATRR140 pKa = 11.84 HH141 pKa = 4.79
Molecular weight: 15.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.909
IPC2_protein 3.757
IPC_protein 3.732
Toseland 3.541
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.872
Wikipedia 3.617
Rodwell 3.567
Grimsley 3.452
Solomon 3.706
Lehninger 3.656
Nozaki 3.821
DTASelect 4.012
Thurlkill 3.579
EMBOSS 3.63
Sillero 3.859
Patrickios 2.587
IPC_peptide 3.706
IPC2_peptide 3.834
IPC2.peptide.svr19 3.779
Protein with the highest isoelectric point:
>tr|A0A261QQ34|A0A261QQ34_9GAMM Thymidine kinase OS=Sodalis-like symbiont of Philaenus spumarius OX=1189304 GN=tdk PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 NRR14 pKa = 11.84 SHH16 pKa = 7.16 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.36 GRR39 pKa = 11.84 TRR41 pKa = 11.84 LTVSSEE47 pKa = 3.72
Molecular weight: 5.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.422
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.31
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.047
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.101
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1600
0
1600
376700
16
1486
235.4
26.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.876 ± 0.081
1.308 ± 0.025
5.374 ± 0.046
5.662 ± 0.059
3.615 ± 0.037
7.319 ± 0.058
2.537 ± 0.034
5.68 ± 0.056
4.133 ± 0.055
10.753 ± 0.083
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.662 ± 0.038
3.294 ± 0.035
4.434 ± 0.044
4.516 ± 0.055
6.715 ± 0.063
5.53 ± 0.042
5.309 ± 0.04
7.181 ± 0.058
1.279 ± 0.029
2.823 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here