Streptococcus phage SW24

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 42 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S7W892|A0A3S7W892_9CAUD DNA methyltransferase OS=Streptococcus phage SW24 OX=2483877 GN=SW24_037 PE=4 SV=1
MM1 pKa = 7.52SVQQSIVNWFVNHH14 pKa = 6.82RR15 pKa = 11.84GKK17 pKa = 9.04LTYY20 pKa = 11.06SMYY23 pKa = 10.77GSRR26 pKa = 11.84NGADD30 pKa = 3.25GTADD34 pKa = 3.34CSGSISQALKK44 pKa = 9.74EE45 pKa = 4.19AGIGIQGLPSTVTLGQQLAKK65 pKa = 10.58NGFYY69 pKa = 10.18RR70 pKa = 11.84VSINQDD76 pKa = 2.14WDD78 pKa = 3.55ALTGDD83 pKa = 4.07IVLMSWGADD92 pKa = 2.98MSTSGGAGGHH102 pKa = 5.75VGVMMDD108 pKa = 3.33ATYY111 pKa = 9.9FISCDD116 pKa = 3.44YY117 pKa = 9.43STQGAPGQAINTYY130 pKa = 8.88PWNDD134 pKa = 3.08YY135 pKa = 10.06YY136 pKa = 11.52AANKK140 pKa = 9.23PSYY143 pKa = 10.16IEE145 pKa = 3.55VWRR148 pKa = 11.84YY149 pKa = 10.13SDD151 pKa = 5.37SAPQTNNQPNTEE163 pKa = 4.11VAPQEE168 pKa = 3.96KK169 pKa = 9.92AYY171 pKa = 11.27YY172 pKa = 8.39EE173 pKa = 4.25ANEE176 pKa = 3.96VKK178 pKa = 10.2YY179 pKa = 11.25VNGIYY184 pKa = 9.81QIKK187 pKa = 9.76CDD189 pKa = 3.74YY190 pKa = 9.43LAPVGFDD197 pKa = 2.79WTEE200 pKa = 3.54NGIPVSMVNWVDD212 pKa = 3.56KK213 pKa = 11.11DD214 pKa = 4.24GNDD217 pKa = 3.97LPDD220 pKa = 4.77GADD223 pKa = 3.18QDD225 pKa = 4.63FKK227 pKa = 11.75AGMFFSFASDD237 pKa = 3.41EE238 pKa = 4.17NNITDD243 pKa = 3.83TGNGGYY249 pKa = 10.4YY250 pKa = 9.8GGYY253 pKa = 8.76YY254 pKa = 9.56FRR256 pKa = 11.84KK257 pKa = 9.96FEE259 pKa = 4.13FGQFGTVWLSCWNKK273 pKa = 10.66DD274 pKa = 3.89DD275 pKa = 5.14LVNYY279 pKa = 7.38YY280 pKa = 8.26QQ281 pKa = 4.22

Molecular weight: 31.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S7W875|A0A3S7W875_9CAUD Uncharacterized protein OS=Streptococcus phage SW24 OX=2483877 GN=SW24_026 PE=4 SV=1
MM1 pKa = 6.97GTTVSVKK8 pKa = 10.13VDD10 pKa = 2.99LHH12 pKa = 7.19GLEE15 pKa = 4.88KK16 pKa = 10.49KK17 pKa = 10.47CSPEE21 pKa = 3.35AVKK24 pKa = 10.43RR25 pKa = 11.84GKK27 pKa = 10.24VAMIGQMITDD37 pKa = 4.47MEE39 pKa = 4.37PFIPRR44 pKa = 11.84RR45 pKa = 11.84DD46 pKa = 3.71GTLSASGSPFSDD58 pKa = 4.77GIRR61 pKa = 11.84YY62 pKa = 7.2PGPYY66 pKa = 10.0ARR68 pKa = 11.84AQFYY72 pKa = 10.7GSSYY76 pKa = 10.12NKK78 pKa = 9.31NRR80 pKa = 11.84SFVFRR85 pKa = 11.84NYY87 pKa = 7.13TTPGTGKK94 pKa = 9.94RR95 pKa = 11.84WDD97 pKa = 3.53MKK99 pKa = 11.21ASAKK103 pKa = 10.21YY104 pKa = 8.51SKK106 pKa = 10.29QWGEE110 pKa = 3.73VALRR114 pKa = 11.84AMGVKK119 pKa = 10.0

Molecular weight: 13.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

42

0

42

9955

47

1528

237.0

26.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.534 ± 1.252

0.542 ± 0.111

5.836 ± 0.652

7.283 ± 0.944

4.229 ± 0.255

6.087 ± 0.686

1.185 ± 0.177

7.233 ± 0.489

8.348 ± 0.748

7.745 ± 0.443

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.953 ± 0.354

5.645 ± 0.375

2.682 ± 0.199

4.591 ± 0.355

3.847 ± 0.483

6.911 ± 0.658

6.298 ± 0.396

5.967 ± 0.274

0.974 ± 0.14

4.108 ± 0.569
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski