Ferroglobus placidus (strain DSM 10642 / AEDII12DO)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales; Archaeoglobaceae; Ferroglobus; Ferroglobus placidus

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2463 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D3RXM1|D3RXM1_FERPA Chemotaxis protein CheA OS=Ferroglobus placidus (strain DSM 10642 / AEDII12DO) OX=589924 GN=Ferp_1075 PE=4 SV=1
MM1 pKa = 7.14GKK3 pKa = 8.85VWKK6 pKa = 10.46VGLAVLVTLLITMATANAIQPSSIFNNSSLTVTIEE41 pKa = 3.96PNPTFAGTPIVSPVNFSVVNAGWYY65 pKa = 10.46GDD67 pKa = 3.49IPITFNVTSPNMGNVTVNVSTDD89 pKa = 3.14LTAYY93 pKa = 10.33NSSTSGTSIMLNLTTEE109 pKa = 3.54TWFAVNASTAGCYY122 pKa = 7.28WVNVSHH128 pKa = 7.79DD129 pKa = 3.71LTNDD133 pKa = 3.2YY134 pKa = 11.69VNFTVCYY141 pKa = 8.41WKK143 pKa = 10.03IDD145 pKa = 3.63SVTLYY150 pKa = 10.62DD151 pKa = 4.17VNGNSYY157 pKa = 11.3DD158 pKa = 3.76LLAGGTQNIEE168 pKa = 3.77AGDD171 pKa = 3.6YY172 pKa = 9.66TIYY175 pKa = 10.78INVTAPSGEE184 pKa = 4.2GYY186 pKa = 10.23EE187 pKa = 4.25VANASLNLTPFGIGWVTLDD206 pKa = 4.44VIDD209 pKa = 4.63SNSTAGTYY217 pKa = 10.18NLSGTIQFHH226 pKa = 6.59KK227 pKa = 10.53ATDD230 pKa = 3.17AGYY233 pKa = 10.6NITGNITIGDD243 pKa = 4.06PVEE246 pKa = 4.45IPAINTSQVDD256 pKa = 3.75VDD258 pKa = 3.83PAPAVKK264 pKa = 9.18WAFLEE269 pKa = 4.3TNPYY273 pKa = 10.92DD274 pKa = 4.53SDD276 pKa = 5.81DD277 pKa = 3.73NVIWDD282 pKa = 4.06GVSDD286 pKa = 4.3YY287 pKa = 11.51INATLYY293 pKa = 10.1VAAVDD298 pKa = 3.59EE299 pKa = 4.77FGNINTSGFSGYY311 pKa = 9.92ISVVSGPGILNKK323 pKa = 10.14TSVNASRR330 pKa = 11.84VDD332 pKa = 3.23AFNISVPNADD342 pKa = 2.65ATAFVTVKK350 pKa = 10.61VYY352 pKa = 11.01GGGLASTTKK361 pKa = 10.2TVGFYY366 pKa = 11.15DD367 pKa = 5.28RR368 pKa = 11.84IDD370 pKa = 3.87HH371 pKa = 7.21LDD373 pKa = 3.48VAVEE377 pKa = 4.12KK378 pKa = 10.54PVLYY382 pKa = 10.79ANNSDD387 pKa = 3.58STTVYY392 pKa = 9.2VTLKK396 pKa = 10.66DD397 pKa = 3.59SAGNTIPVEE406 pKa = 4.48GINITIDD413 pKa = 3.17EE414 pKa = 4.66LTTLGLDD421 pKa = 3.37IPTSTNATDD430 pKa = 3.51NSGVATFTVTAGANSGTATIGAIVDD455 pKa = 4.32SGIAWDD461 pKa = 4.5GRR463 pKa = 11.84YY464 pKa = 10.28GDD466 pKa = 4.92ANITLKK472 pKa = 10.44QAPDD476 pKa = 3.4TTAVQTAATSAVPSTITAGEE496 pKa = 4.57GYY498 pKa = 10.0KK499 pKa = 9.78IEE501 pKa = 4.23FTIVDD506 pKa = 3.67HH507 pKa = 6.99AGQPIANSAEE517 pKa = 4.01FPVKK521 pKa = 10.45FNITAGDD528 pKa = 4.04AVWLEE533 pKa = 4.05NNAKK537 pKa = 10.11VLFVNATDD545 pKa = 3.86ANGNVSATLYY555 pKa = 8.81STNASTSINVNISIKK570 pKa = 10.54NEE572 pKa = 3.59SGQWVTLVPDD582 pKa = 3.79TPVNVDD588 pKa = 3.44PAEE591 pKa = 4.12VADD594 pKa = 5.11FVVEE598 pKa = 3.99PDD600 pKa = 4.11SIGLPAVIGASEE612 pKa = 4.57VINVTLVDD620 pKa = 3.84AYY622 pKa = 11.08GNVNSTNATMIVTTDD637 pKa = 3.44NPSLGLLNNGTNHH650 pKa = 7.28PDD652 pKa = 3.61SIVVSITGGKK662 pKa = 10.45GSFTYY667 pKa = 10.34YY668 pKa = 10.65VNSSTPGVANLTLNVTDD685 pKa = 3.93FNIVKK690 pKa = 8.55TVKK693 pKa = 10.08IVTTAPQGVVLTFDD707 pKa = 3.04QTLPFVASDD716 pKa = 3.48INVTAQVTTAEE727 pKa = 4.3GLPLKK732 pKa = 10.4YY733 pKa = 10.74SNVDD737 pKa = 3.14LTLVVKK743 pKa = 10.9NPDD746 pKa = 3.19GNVIFIGTASTNASGAAVWTLNGTNFTAPGVYY778 pKa = 8.81TFKK781 pKa = 10.99VYY783 pKa = 8.83NTSYY787 pKa = 10.81GISDD791 pKa = 3.88TKK793 pKa = 10.97QLTFAGPAVKK803 pKa = 10.47VVVTVNNTAPLVNDD817 pKa = 3.63TVMITATFYY826 pKa = 11.22DD827 pKa = 3.96VNDD830 pKa = 4.42LVTSDD835 pKa = 5.12LDD837 pKa = 3.93GADD840 pKa = 3.34VTFLINDD847 pKa = 3.79VVVGTATITNGVASINYY864 pKa = 7.22TFTEE868 pKa = 4.21AGTFNITAFYY878 pKa = 10.77NATLQDD884 pKa = 4.1TIAITVVTTVAPGDD898 pKa = 3.84SDD900 pKa = 4.08NDD902 pKa = 3.33GWNDD906 pKa = 3.39SVEE909 pKa = 4.1ALIASYY915 pKa = 11.04NPSFDD920 pKa = 3.71PAQVPTQDD928 pKa = 3.79DD929 pKa = 4.13VINAVVGAVNKK940 pKa = 10.38YY941 pKa = 9.76FDD943 pKa = 4.11PATTEE948 pKa = 3.99SEE950 pKa = 4.01KK951 pKa = 10.58QQIVNDD957 pKa = 4.59IITLVQEE964 pKa = 4.41YY965 pKa = 9.93FLHH968 pKa = 6.69FF969 pKa = 4.1

Molecular weight: 101.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D3S0Z0|D3S0Z0_FERPA CRM domain-containing protein OS=Ferroglobus placidus (strain DSM 10642 / AEDII12DO) OX=589924 GN=Ferp_0034 PE=4 SV=1
MM1 pKa = 7.34GKK3 pKa = 8.36KK4 pKa = 8.33TVGVKK9 pKa = 10.11KK10 pKa = 10.67RR11 pKa = 11.84LGKK14 pKa = 9.83FLKK17 pKa = 10.06QNRR20 pKa = 11.84RR21 pKa = 11.84PPVWVILKK29 pKa = 6.93TKK31 pKa = 10.0RR32 pKa = 11.84RR33 pKa = 11.84VVTSPKK39 pKa = 9.41RR40 pKa = 11.84RR41 pKa = 11.84FWRR44 pKa = 11.84HH45 pKa = 4.28TKK47 pKa = 10.74LKK49 pKa = 10.26VV50 pKa = 2.91

Molecular weight: 6.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2463

0

2463

660597

31

1864

268.2

30.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.075 ± 0.053

1.128 ± 0.028

4.758 ± 0.033

9.528 ± 0.081

4.764 ± 0.045

6.73 ± 0.04

1.487 ± 0.021

7.697 ± 0.049

8.438 ± 0.056

9.446 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.2 ± 0.022

3.387 ± 0.033

3.687 ± 0.028

1.5 ± 0.02

5.252 ± 0.048

5.525 ± 0.048

3.965 ± 0.043

8.579 ± 0.044

1.035 ± 0.024

3.821 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski