Flavimaricola marinus
Average proteome isoelectric point is 5.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4548 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A238LFH6|A0A238LFH6_9RHOB Carnitinyl-CoA dehydratase OS=Flavimaricola marinus OX=1819565 GN=caiD_1 PE=3 SV=1
MM1 pKa = 6.2 VTGVEE6 pKa = 4.07 LTYY9 pKa = 10.11 DD10 pKa = 3.58 TGADD14 pKa = 3.31 AMEE17 pKa = 4.09 MAEE20 pKa = 5.06 AIFGDD25 pKa = 3.99 SVEE28 pKa = 4.53 VVSASYY34 pKa = 10.47 SGSYY38 pKa = 9.69 YY39 pKa = 10.84 SSAIYY44 pKa = 10.3 TNGEE48 pKa = 4.29 SISPNVVPGDD58 pKa = 3.5 TGVILSTGAATYY70 pKa = 7.5 FTNSGGGSNDD80 pKa = 3.47 YY81 pKa = 11.54 SNTSYY86 pKa = 11.61 NSGGEE91 pKa = 4.09 NYY93 pKa = 10.13 NDD95 pKa = 4.0 SFDD98 pKa = 4.93 DD99 pKa = 3.64 VAGTYY104 pKa = 9.47 TYY106 pKa = 11.03 DD107 pKa = 3.29 AAYY110 pKa = 9.01 MDD112 pKa = 4.22 IVFIPDD118 pKa = 3.38 GDD120 pKa = 4.02 VMTMQFVFASDD131 pKa = 4.29 EE132 pKa = 4.12 YY133 pKa = 10.77 PEE135 pKa = 4.49 YY136 pKa = 10.88 SDD138 pKa = 5.55 SIYY141 pKa = 11.06 NDD143 pKa = 3.16 VVLVEE148 pKa = 4.42 VNGEE152 pKa = 4.19 VIPLSVTNSSTSVTEE167 pKa = 3.7 INQNEE172 pKa = 4.43 NVNLYY177 pKa = 10.5 NDD179 pKa = 3.84 NTADD183 pKa = 3.52 QYY185 pKa = 9.5 NTEE188 pKa = 4.03 MDD190 pKa = 3.79 GFTVTMTLTIPVVAGEE206 pKa = 4.24 EE207 pKa = 3.74 NSIRR211 pKa = 11.84 IGIADD216 pKa = 4.28 ASDD219 pKa = 3.49 SNYY222 pKa = 10.97 DD223 pKa = 3.68 SNLLIAGDD231 pKa = 4.04 SLQTDD236 pKa = 4.22 LVAIQDD242 pKa = 3.86 DD243 pKa = 4.39 VTVKK247 pKa = 10.76 EE248 pKa = 4.77 GVTKK252 pKa = 10.21 TVDD255 pKa = 3.72 VLDD258 pKa = 4.24 NDD260 pKa = 4.48 LNATSGTLVITHH272 pKa = 6.87 INDD275 pKa = 3.18 IAVTAGDD282 pKa = 4.21 TVNLTTGQSVTLNADD297 pKa = 3.25 GTLSITADD305 pKa = 3.47 SDD307 pKa = 3.54 NDD309 pKa = 4.23 TVSFTYY315 pKa = 8.58 EE316 pKa = 3.45 TAAEE320 pKa = 4.51 DD321 pKa = 3.81 GSGAVLEE328 pKa = 4.34 TDD330 pKa = 3.42 VGFVTVTTVPCFVAGTRR347 pKa = 11.84 ISTPDD352 pKa = 2.93 GDD354 pKa = 4.44 RR355 pKa = 11.84 RR356 pKa = 11.84 VEE358 pKa = 3.96 DD359 pKa = 4.34 LQPGDD364 pKa = 4.21 LVLTKK369 pKa = 10.57 DD370 pKa = 3.69 AGAQQVRR377 pKa = 11.84 WAGRR381 pKa = 11.84 RR382 pKa = 11.84 SVAAQGRR389 pKa = 11.84 MAPVLIRR396 pKa = 11.84 SGTFGTHH403 pKa = 5.6 GDD405 pKa = 3.72 VMVSPLHH412 pKa = 6.03 RR413 pKa = 11.84 VLVTGGVAEE422 pKa = 4.61 LLFAEE427 pKa = 4.68 PEE429 pKa = 4.37 VLVTARR435 pKa = 11.84 DD436 pKa = 3.73 LVNDD440 pKa = 3.94 RR441 pKa = 11.84 SVRR444 pKa = 11.84 RR445 pKa = 11.84 VEE447 pKa = 4.45 GGTVDD452 pKa = 3.41 YY453 pKa = 10.72 VHH455 pKa = 8.03 IMFDD459 pKa = 3.18 AHH461 pKa = 5.35 QVIYY465 pKa = 10.9 ADD467 pKa = 3.95 GLATEE472 pKa = 4.9 SFLPGPQTTSLFEE485 pKa = 4.27 DD486 pKa = 4.27 EE487 pKa = 5.34 IVAEE491 pKa = 4.16 ICAIFPEE498 pKa = 4.74 LDD500 pKa = 3.33 PVTGEE505 pKa = 4.56 GYY507 pKa = 10.4 SPAARR512 pKa = 11.84 RR513 pKa = 11.84 TLRR516 pKa = 11.84 AYY518 pKa = 9.73 EE519 pKa = 4.08 GRR521 pKa = 11.84 LLGQVAA527 pKa = 3.83
Molecular weight: 55.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.696
IPC2_protein 3.694
IPC_protein 3.732
Toseland 3.503
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.554
Grimsley 3.414
Solomon 3.719
Lehninger 3.681
Nozaki 3.834
DTASelect 4.088
Thurlkill 3.554
EMBOSS 3.668
Sillero 3.859
Patrickios 0.947
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.759
Protein with the highest isoelectric point:
>tr|A0A238L9L3|A0A238L9L3_9RHOB Uncharacterized protein OS=Flavimaricola marinus OX=1819565 GN=LOM8899_00414 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 VRR12 pKa = 11.84 KK13 pKa = 9.05 ARR15 pKa = 11.84 HH16 pKa = 4.79 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATKK25 pKa = 10.45 AGRR28 pKa = 11.84 KK29 pKa = 8.81 IINARR34 pKa = 11.84 RR35 pKa = 11.84 AQGRR39 pKa = 11.84 SKK41 pKa = 11.13 LSAA44 pKa = 3.7
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4548
0
4548
1434317
29
2947
315.4
34.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.253 ± 0.057
0.896 ± 0.01
6.228 ± 0.039
5.747 ± 0.033
3.709 ± 0.025
8.694 ± 0.038
2.037 ± 0.017
5.323 ± 0.027
2.903 ± 0.031
9.93 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.818 ± 0.017
2.582 ± 0.019
5.222 ± 0.028
3.178 ± 0.018
6.489 ± 0.038
5.344 ± 0.029
5.643 ± 0.028
7.321 ± 0.03
1.437 ± 0.016
2.247 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here