Bovine faeces associated circular DNA virus 2

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A168MFX8|A0A168MFX8_9VIRU Capsid protein OS=Bovine faeces associated circular DNA virus 2 OX=1843765 PE=4 SV=1
MM1 pKa = 7.47SVRR4 pKa = 11.84DD5 pKa = 3.97YY6 pKa = 11.16RR7 pKa = 11.84SKK9 pKa = 11.28NYY11 pKa = 10.27LLTYY15 pKa = 8.41PRR17 pKa = 11.84CGLQQWWVLTNLQNNEE33 pKa = 4.37RR34 pKa = 11.84IKK36 pKa = 10.5MIIQYY41 pKa = 9.97VIVAQEE47 pKa = 3.81NHH49 pKa = 5.65QDD51 pKa = 3.7GAEE54 pKa = 4.43HH55 pKa = 5.95IHH57 pKa = 6.25VFLALTEE64 pKa = 4.54PIRR67 pKa = 11.84IKK69 pKa = 10.84SNEE72 pKa = 3.81MDD74 pKa = 3.25VFDD77 pKa = 5.62IVDD80 pKa = 3.41EE81 pKa = 4.58DD82 pKa = 4.19EE83 pKa = 4.38PDD85 pKa = 3.2KK86 pKa = 11.01RR87 pKa = 11.84VYY89 pKa = 10.09HH90 pKa = 6.55CNIQSVKK97 pKa = 10.29SPKK100 pKa = 10.16DD101 pKa = 3.35AIQYY105 pKa = 7.82VKK107 pKa = 10.94KK108 pKa = 9.7NGHH111 pKa = 6.84FITYY115 pKa = 7.76GICPWKK121 pKa = 10.87VPLTMAEE128 pKa = 4.31KK129 pKa = 11.09NEE131 pKa = 3.88LLQSKK136 pKa = 9.54SLHH139 pKa = 5.75EE140 pKa = 4.09LVEE143 pKa = 4.37SGMVSIFKK151 pKa = 10.32IPQLQKK157 pKa = 10.86AISILQNEE165 pKa = 4.82LGNVEE170 pKa = 4.02RR171 pKa = 11.84QPPKK175 pKa = 8.47VHH177 pKa = 6.78WYY179 pKa = 9.64YY180 pKa = 11.5GSTGTGKK187 pKa = 8.11TRR189 pKa = 11.84TAYY192 pKa = 10.07EE193 pKa = 3.94EE194 pKa = 4.24AKK196 pKa = 10.46EE197 pKa = 4.33SEE199 pKa = 3.88DD200 pKa = 4.42GYY202 pKa = 10.34WISHH206 pKa = 5.23EE207 pKa = 4.1TGEE210 pKa = 4.47WFDD213 pKa = 4.39GYY215 pKa = 9.91IGQGIAILDD224 pKa = 4.7DD225 pKa = 3.05IRR227 pKa = 11.84AATWPLQKK235 pKa = 10.63LLRR238 pKa = 11.84LCDD241 pKa = 3.95RR242 pKa = 11.84YY243 pKa = 10.25PIQVPIKK250 pKa = 10.79GGFTWWRR257 pKa = 11.84PRR259 pKa = 11.84EE260 pKa = 4.03IYY262 pKa = 8.78ITAPGRR268 pKa = 11.84PEE270 pKa = 5.81DD271 pKa = 3.3IYY273 pKa = 11.75KK274 pKa = 10.68NYY276 pKa = 7.75TTGQPYY282 pKa = 11.2DD283 pKa = 3.97NIEE286 pKa = 3.86QLNRR290 pKa = 11.84RR291 pKa = 11.84ITEE294 pKa = 4.14MIDD297 pKa = 3.26FDD299 pKa = 5.54SEE301 pKa = 4.51TEE303 pKa = 4.74PIDD306 pKa = 3.39QTLYY310 pKa = 10.21WKK312 pKa = 10.71GG313 pKa = 3.26

Molecular weight: 36.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A168MFX8|A0A168MFX8_9VIRU Capsid protein OS=Bovine faeces associated circular DNA virus 2 OX=1843765 PE=4 SV=1
MM1 pKa = 8.04PIHH4 pKa = 7.1AIISPKK10 pKa = 8.68FTYY13 pKa = 9.31GQGYY17 pKa = 7.65NHH19 pKa = 5.59VQIRR23 pKa = 11.84VTANSTNAWKK33 pKa = 10.79NGMFDD38 pKa = 3.39YY39 pKa = 10.87SSRR42 pKa = 11.84TDD44 pKa = 2.97TEE46 pKa = 4.27MAQHH50 pKa = 6.55VIMPNIEE57 pKa = 3.99FPVIGNGHH65 pKa = 6.3SNRR68 pKa = 11.84KK69 pKa = 8.65SNRR72 pKa = 11.84LTVSSIRR79 pKa = 11.84VKK81 pKa = 10.69MNFCMDD87 pKa = 4.13GEE89 pKa = 4.54CLRR92 pKa = 11.84KK93 pKa = 9.87FNWFSSAEE101 pKa = 4.24TVTGSSQLPLNPHH114 pKa = 6.76LFLKK118 pKa = 10.61FRR120 pKa = 11.84LFLLSVDD127 pKa = 4.26DD128 pKa = 5.32DD129 pKa = 4.39IEE131 pKa = 4.25VSRR134 pKa = 11.84NKK136 pKa = 10.3LLNWFLATHH145 pKa = 6.1CMYY148 pKa = 10.52RR149 pKa = 11.84YY150 pKa = 6.49PTVEE154 pKa = 4.22EE155 pKa = 4.26VLKK158 pKa = 10.9GPSDD162 pKa = 3.61TAVTDD167 pKa = 3.78TPGPTSVHH175 pKa = 5.92SSVLRR180 pKa = 11.84VTTDD184 pKa = 2.51WTGKK188 pKa = 10.37FNVLADD194 pKa = 3.88RR195 pKa = 11.84KK196 pKa = 9.18FTISAKK202 pKa = 10.35NPQFDD207 pKa = 3.45VDD209 pKa = 3.55MTIPLRR215 pKa = 11.84KK216 pKa = 9.67EE217 pKa = 3.74FVFDD221 pKa = 3.79EE222 pKa = 4.9NYY224 pKa = 10.66DD225 pKa = 3.62ATSKK229 pKa = 10.91LLYY232 pKa = 9.28PKK234 pKa = 10.1LYY236 pKa = 10.88LFILPPLSWEE246 pKa = 3.98VDD248 pKa = 3.54CDD250 pKa = 3.58VMTSYY255 pKa = 10.78FCKK258 pKa = 10.12HH259 pKa = 5.4YY260 pKa = 10.44QSSSAIAPLCFHH272 pKa = 6.84VYY274 pKa = 9.07SWTKK278 pKa = 11.07LNFVDD283 pKa = 5.26LL284 pKa = 4.3

Molecular weight: 32.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

597

284

313

298.5

34.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.02 ± 0.129

1.675 ± 0.274

5.863 ± 0.077

5.863 ± 1.466

4.355 ± 1.462

4.858 ± 0.837

2.848 ± 0.201

7.203 ± 1.644

6.03 ± 0.248

7.873 ± 0.582

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.513 ± 0.411

5.193 ± 0.497

5.528 ± 0.066

4.188 ± 1.299

4.69 ± 0.291

6.7 ± 1.537

6.7 ± 0.655

6.365 ± 0.865

2.68 ± 0.355

4.858 ± 0.616
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski