Avibacterium paragallinarum (Haemophilus gallinarum)
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2171 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F5EPP6|A0A0F5EPP6_AVIPA YcfA-like protein OS=Avibacterium paragallinarum OX=728 GN=NCTC10926_01266 PE=3 SV=1
MM1 pKa = 7.62 CIDD4 pKa = 4.07 TSTLCDD10 pKa = 3.15 IYY12 pKa = 10.87 LDD14 pKa = 3.56 QVDD17 pKa = 4.07 VVEE20 pKa = 5.42 PIFSNFGGLTSFHH33 pKa = 6.44 GKK35 pKa = 7.51 VTTVKK40 pKa = 10.41 CFEE43 pKa = 4.51 NNGLIAEE50 pKa = 4.16 ILEE53 pKa = 4.34 EE54 pKa = 4.18 NGEE57 pKa = 4.18 GRR59 pKa = 11.84 VLLVDD64 pKa = 3.54 GGGAVRR70 pKa = 11.84 QALLDD75 pKa = 3.87 AEE77 pKa = 4.58 LAQLAADD84 pKa = 4.38 NGWEE88 pKa = 4.36 GIIVYY93 pKa = 9.79 GAVRR97 pKa = 11.84 QIQQLEE103 pKa = 4.23 NIDD106 pKa = 3.7 IGIQALAPIPVGANSDD122 pKa = 3.67 TQGEE126 pKa = 3.91 VDD128 pKa = 3.33 IPVNFGGVTFFPEE141 pKa = 4.3 DD142 pKa = 3.43 YY143 pKa = 10.81 VYY145 pKa = 11.59 ADD147 pKa = 3.49 LTGIILSQEE156 pKa = 4.03 PLDD159 pKa = 5.57 LEE161 pKa = 4.93 DD162 pKa = 3.68 LTEE165 pKa = 3.93
Molecular weight: 17.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.782
IPC2_protein 3.579
IPC_protein 3.541
Toseland 3.338
ProMoST 3.694
Dawson 3.541
Bjellqvist 3.745
Wikipedia 3.465
Rodwell 3.376
Grimsley 3.261
Solomon 3.516
Lehninger 3.478
Nozaki 3.668
DTASelect 3.846
Thurlkill 3.401
EMBOSS 3.478
Sillero 3.668
Patrickios 0.833
IPC_peptide 3.516
IPC2_peptide 3.643
IPC2.peptide.svr19 3.694
Protein with the highest isoelectric point:
>tr|A0A0F5EW23|A0A0F5EW23_AVIPA Mu-like prophage FluMu protein gp16 OS=Avibacterium paragallinarum OX=728 GN=Z012_11315 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 SRR14 pKa = 11.84 THH16 pKa = 6.4 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.15 GRR39 pKa = 11.84 KK40 pKa = 8.87 SLSAA44 pKa = 3.86
Molecular weight: 5.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2171
0
2171
635325
32
2196
292.6
32.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.369 ± 0.058
1.047 ± 0.019
4.805 ± 0.039
6.402 ± 0.055
4.36 ± 0.04
6.633 ± 0.055
2.126 ± 0.027
6.941 ± 0.049
6.174 ± 0.049
10.636 ± 0.066
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.429 ± 0.025
4.731 ± 0.044
3.815 ± 0.03
5.358 ± 0.062
4.602 ± 0.04
5.648 ± 0.039
5.096 ± 0.034
6.389 ± 0.053
1.173 ± 0.021
3.266 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here