Bovine adenovirus 6
Average proteome isoelectric point is 7.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 30 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K9MPD6|K9MPD6_9ADEN ORF2 OS=Bovine adenovirus 6 OX=111167 PE=4 SV=1
MM1 pKa = 6.99 NQLTRR6 pKa = 11.84 TFMGAAEE13 pKa = 4.19 LVSYY17 pKa = 7.89 FTDD20 pKa = 3.14 INTNQYY26 pKa = 11.17 AIYY29 pKa = 10.24 CIATSKK35 pKa = 10.69 SFNYY39 pKa = 7.98 WAKK42 pKa = 10.71 GPFRR46 pKa = 11.84 MALRR50 pKa = 11.84 FIGDD54 pKa = 3.32 TTVYY58 pKa = 10.94 VLAQKK63 pKa = 10.81 DD64 pKa = 3.93 LFPEE68 pKa = 4.39 FDD70 pKa = 4.16 ALDD73 pKa = 3.72 FLKK76 pKa = 10.23 WANFSNMAIGFFPVEE91 pKa = 4.01 WEE93 pKa = 3.94 LLINSLVDD101 pKa = 3.67 RR102 pKa = 11.84 PMLTAEE108 pKa = 4.31 VVLIPGDD115 pKa = 3.55 VRR117 pKa = 11.84 HH118 pKa = 6.12 EE119 pKa = 3.82 SWEE122 pKa = 3.84 FFKK125 pKa = 11.09 YY126 pKa = 9.72 YY127 pKa = 8.86 WEE129 pKa = 4.33 NYY131 pKa = 8.8 FPYY134 pKa = 10.4 FPIAPDD140 pKa = 3.33 WEE142 pKa = 4.3
Molecular weight: 16.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.355
IPC2_protein 4.571
IPC_protein 4.444
Toseland 4.279
ProMoST 4.482
Dawson 4.393
Bjellqvist 4.609
Wikipedia 4.279
Rodwell 4.279
Grimsley 4.19
Solomon 4.393
Lehninger 4.342
Nozaki 4.507
DTASelect 4.66
Thurlkill 4.291
EMBOSS 4.291
Sillero 4.558
Patrickios 2.969
IPC_peptide 4.393
IPC2_peptide 4.546
IPC2.peptide.svr19 4.495
Protein with the highest isoelectric point:
>tr|K9MPD6|K9MPD6_9ADEN ORF2 OS=Bovine adenovirus 6 OX=111167 PE=4 SV=1
MM1 pKa = 7.17 KK2 pKa = 7.71 TTSGKK7 pKa = 10.75 AKK9 pKa = 10.33 VKK11 pKa = 10.24 LAMSRR16 pKa = 11.84 LVRR19 pKa = 11.84 YY20 pKa = 9.57 KK21 pKa = 10.56 KK22 pKa = 10.26 ILIQISSGSNDD33 pKa = 2.66 TRR35 pKa = 11.84 VPPEE39 pKa = 3.77 SYY41 pKa = 8.75 KK42 pKa = 10.35 TIKK45 pKa = 10.14 KK46 pKa = 9.97 SRR48 pKa = 11.84 STTAQKK54 pKa = 10.57 KK55 pKa = 7.26 QANKK59 pKa = 10.25 EE60 pKa = 4.16 KK61 pKa = 10.65 IFF63 pKa = 3.84
Molecular weight: 7.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.207
IPC2_protein 10.072
IPC_protein 10.379
Toseland 11.096
ProMoST 10.599
Dawson 11.155
Bjellqvist 10.76
Wikipedia 11.286
Rodwell 11.769
Grimsley 11.184
Solomon 11.228
Lehninger 11.213
Nozaki 11.052
DTASelect 10.76
Thurlkill 11.067
EMBOSS 11.477
Sillero 11.082
Patrickios 11.506
IPC_peptide 11.242
IPC2_peptide 9.224
IPC2.peptide.svr19 8.447
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
30
0
30
8859
54
1073
295.3
33.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.407 ± 0.378
2.37 ± 0.51
4.651 ± 0.213
5.655 ± 0.454
5.294 ± 0.26
4.933 ± 0.377
2.246 ± 0.25
5.915 ± 0.27
6.581 ± 0.754
10.08 ± 0.53
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.133 ± 0.188
6.344 ± 0.422
5.317 ± 0.311
4.459 ± 0.27
4.707 ± 0.451
7.157 ± 0.296
6.05 ± 0.409
4.967 ± 0.346
1.456 ± 0.146
4.278 ± 0.345
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here