Pseudonocardia ammonioxydans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria;

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6995 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I4VJS5|A0A1I4VJS5_9PSEU Precorrin-8X methylmutase OS=Pseudonocardia ammonioxydans OX=260086 GN=SAMN05216207_1006119 PE=3 SV=1
MM1 pKa = 7.42SVGSAAPTVPQARR14 pKa = 11.84RR15 pKa = 11.84WFCEE19 pKa = 3.56PCGFVYY25 pKa = 10.52DD26 pKa = 4.37PAEE29 pKa = 4.48GDD31 pKa = 3.49PDD33 pKa = 4.06SGIEE37 pKa = 3.76PGTPFEE43 pKa = 5.2EE44 pKa = 5.54IPDD47 pKa = 3.6DD48 pKa = 4.02WMCPICGATKK58 pKa = 10.56ADD60 pKa = 3.75FRR62 pKa = 11.84EE63 pKa = 4.39LGPDD67 pKa = 3.12EE68 pKa = 4.92TFPGMM73 pKa = 4.27

Molecular weight: 7.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I5F8X9|A0A1I5F8X9_9PSEU 2-Methylisocitrate lyase PEP mutase family OS=Pseudonocardia ammonioxydans OX=260086 GN=SAMN05216207_103620 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.45VHH17 pKa = 5.31GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.74GRR40 pKa = 11.84SSVSAA45 pKa = 3.76

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6995

0

6995

2185542

24

6523

312.4

33.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.24 ± 0.044

0.767 ± 0.008

6.396 ± 0.027

5.485 ± 0.026

2.539 ± 0.018

10.09 ± 0.034

2.289 ± 0.015

3.024 ± 0.019

1.268 ± 0.015

10.179 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.654 ± 0.012

1.454 ± 0.013

6.527 ± 0.029

2.418 ± 0.018

8.539 ± 0.032

4.741 ± 0.024

6.1 ± 0.019

9.103 ± 0.03

1.444 ± 0.011

1.743 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski