Enterobacter phage Tyrion

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Uetakevirus; unclassified Uetakevirus

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A193GYW1|A0A193GYW1_9CAUD Putative transcriptional regulator OS=Enterobacter phage Tyrion OX=1864623 GN=BI095_gp34 PE=4 SV=1
MM1 pKa = 7.7ICQTCGGQVFWKK13 pKa = 10.33GPWSSLTHH21 pKa = 5.67TEE23 pKa = 4.32CEE25 pKa = 4.11SCGAINNQSVDD36 pKa = 3.74EE37 pKa = 4.76PDD39 pKa = 3.59DD40 pKa = 4.58SEE42 pKa = 4.98VEE44 pKa = 4.16EE45 pKa = 4.37

Molecular weight: 4.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A193GYK6|A0A193GYK6_9CAUD Uncharacterized protein OS=Enterobacter phage Tyrion OX=1864623 GN=BI095_gp41 PE=4 SV=1
MM1 pKa = 6.55TTHH4 pKa = 6.96EE5 pKa = 4.9LLLLIANAVICSGIAIRR22 pKa = 11.84VGTFRR27 pKa = 11.84RR28 pKa = 11.84NGSQHH33 pKa = 6.14RR34 pKa = 11.84RR35 pKa = 11.84WGGWIAYY42 pKa = 7.63FLIVAAASIPIRR54 pKa = 11.84AAYY57 pKa = 9.02AIWYY61 pKa = 5.87HH62 pKa = 5.56TPMAADD68 pKa = 3.93LSEE71 pKa = 4.21VVINAVMLAAVLKK84 pKa = 9.37TRR86 pKa = 11.84GNVVQIFKK94 pKa = 10.4ISRR97 pKa = 11.84SQHH100 pKa = 3.2GHH102 pKa = 4.83

Molecular weight: 11.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

12990

38

852

232.0

25.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.854 ± 0.436

1.032 ± 0.114

6.105 ± 0.223

5.735 ± 0.348

3.387 ± 0.217

7.436 ± 0.339

1.594 ± 0.141

5.643 ± 0.337

5.258 ± 0.357

7.706 ± 0.354

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.025 ± 0.253

4.681 ± 0.212

4.157 ± 0.237

4.834 ± 0.373

5.851 ± 0.261

6.659 ± 0.249

5.666 ± 0.358

6.405 ± 0.269

1.747 ± 0.146

3.226 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski