Amino acid dipepetide frequency for Mycobacterium phage Tortellini

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.893AlaAla: 17.893 ± 1.413
0.888AlaCys: 0.888 ± 0.229
7.297AlaAsp: 7.297 ± 0.77
8.947AlaGlu: 8.947 ± 1.017
2.792AlaPhe: 2.792 ± 0.582
9.391AlaGly: 9.391 ± 0.873
1.904AlaHis: 1.904 ± 0.374
6.218AlaIle: 6.218 ± 0.62
4.251AlaLys: 4.251 ± 0.71
10.47AlaLeu: 10.47 ± 0.778
2.475AlaMet: 2.475 ± 0.351
3.236AlaAsn: 3.236 ± 0.415
6.218AlaPro: 6.218 ± 0.879
6.028AlaGln: 6.028 ± 0.659
8.439AlaArg: 8.439 ± 0.853
6.853AlaSer: 6.853 ± 0.759
6.662AlaThr: 6.662 ± 0.731
7.741AlaVal: 7.741 ± 0.688
1.396AlaTrp: 1.396 ± 0.334
2.919AlaTyr: 2.919 ± 0.385
0.0AlaXaa: 0.0 ± 0.0
Cys
1.015CysAla: 1.015 ± 0.224
0.127CysCys: 0.127 ± 0.082
0.888CysAsp: 0.888 ± 0.223
0.825CysGlu: 0.825 ± 0.215
0.19CysPhe: 0.19 ± 0.105
1.713CysGly: 1.713 ± 0.355
0.444CysHis: 0.444 ± 0.152
0.317CysIle: 0.317 ± 0.151
0.317CysLys: 0.317 ± 0.153
0.317CysLeu: 0.317 ± 0.159
0.127CysMet: 0.127 ± 0.087
0.254CysAsn: 0.254 ± 0.116
0.888CysPro: 0.888 ± 0.222
0.254CysGln: 0.254 ± 0.146
1.015CysArg: 1.015 ± 0.246
0.571CysSer: 0.571 ± 0.252
0.571CysThr: 0.571 ± 0.185
0.19CysVal: 0.19 ± 0.121
0.127CysTrp: 0.127 ± 0.089
0.254CysTyr: 0.254 ± 0.125
0.0CysXaa: 0.0 ± 0.0
Asp
8.566AspAla: 8.566 ± 0.702
0.571AspCys: 0.571 ± 0.189
4.822AspAsp: 4.822 ± 0.624
5.52AspGlu: 5.52 ± 0.661
1.713AspPhe: 1.713 ± 0.263
6.726AspGly: 6.726 ± 0.672
1.332AspHis: 1.332 ± 0.324
2.348AspIle: 2.348 ± 0.408
1.523AspLys: 1.523 ± 0.229
5.013AspLeu: 5.013 ± 0.601
1.459AspMet: 1.459 ± 0.226
1.586AspAsn: 1.586 ± 0.308
5.33AspPro: 5.33 ± 0.542
1.967AspGln: 1.967 ± 0.367
4.061AspArg: 4.061 ± 0.582
3.363AspSer: 3.363 ± 0.654
3.553AspThr: 3.553 ± 0.468
4.442AspVal: 4.442 ± 0.51
1.332AspTrp: 1.332 ± 0.336
1.269AspTyr: 1.269 ± 0.311
0.0AspXaa: 0.0 ± 0.0
Glu
7.36GluAla: 7.36 ± 0.976
0.888GluCys: 0.888 ± 0.229
3.553GluAsp: 3.553 ± 0.483
3.109GluGlu: 3.109 ± 0.508
2.157GluPhe: 2.157 ± 0.377
3.617GluGly: 3.617 ± 0.451
1.396GluHis: 1.396 ± 0.324
3.553GluIle: 3.553 ± 0.538
1.84GluLys: 1.84 ± 0.332
6.853GluLeu: 6.853 ± 0.852
1.777GluMet: 1.777 ± 0.315
1.396GluAsn: 1.396 ± 0.355
3.363GluPro: 3.363 ± 0.546
2.919GluGln: 2.919 ± 0.486
4.569GluArg: 4.569 ± 0.557
3.109GluSer: 3.109 ± 0.431
3.236GluThr: 3.236 ± 0.409
5.14GluVal: 5.14 ± 0.598
1.65GluTrp: 1.65 ± 0.288
1.206GluTyr: 1.206 ± 0.27
0.0GluXaa: 0.0 ± 0.0
Phe
2.982PheAla: 2.982 ± 0.476
0.063PheCys: 0.063 ± 0.068
2.792PheAsp: 2.792 ± 0.498
2.221PheGlu: 2.221 ± 0.369
0.761PhePhe: 0.761 ± 0.196
3.046PheGly: 3.046 ± 0.517
0.381PheHis: 0.381 ± 0.144
1.65PheIle: 1.65 ± 0.395
1.396PheLys: 1.396 ± 0.308
2.538PheLeu: 2.538 ± 0.33
0.698PheMet: 0.698 ± 0.19
0.571PheAsn: 0.571 ± 0.158
1.777PhePro: 1.777 ± 0.326
0.444PheGln: 0.444 ± 0.178
1.65PheArg: 1.65 ± 0.303
1.142PheSer: 1.142 ± 0.244
1.84PheThr: 1.84 ± 0.318
2.284PheVal: 2.284 ± 0.318
0.254PheTrp: 0.254 ± 0.109
0.698PheTyr: 0.698 ± 0.19
0.0PheXaa: 0.0 ± 0.0
Gly
9.201GlyAla: 9.201 ± 1.435
1.015GlyCys: 1.015 ± 0.276
5.14GlyAsp: 5.14 ± 0.453
5.076GlyGlu: 5.076 ± 0.624
2.919GlyPhe: 2.919 ± 0.686
10.406GlyGly: 10.406 ± 2.14
1.142GlyHis: 1.142 ± 0.272
4.315GlyIle: 4.315 ± 0.599
3.236GlyLys: 3.236 ± 0.399
7.36GlyLeu: 7.36 ± 0.852
1.269GlyMet: 1.269 ± 0.306
3.236GlyAsn: 3.236 ± 0.694
4.315GlyPro: 4.315 ± 0.65
3.49GlyGln: 3.49 ± 0.727
5.711GlyArg: 5.711 ± 0.613
5.076GlySer: 5.076 ± 0.749
5.964GlyThr: 5.964 ± 0.85
6.916GlyVal: 6.916 ± 0.637
1.904GlyTrp: 1.904 ± 0.312
2.792GlyTyr: 2.792 ± 0.399
0.0GlyXaa: 0.0 ± 0.0
His
1.904HisAla: 1.904 ± 0.329
0.127HisCys: 0.127 ± 0.101
1.523HisAsp: 1.523 ± 0.304
1.142HisGlu: 1.142 ± 0.285
0.635HisPhe: 0.635 ± 0.218
1.904HisGly: 1.904 ± 0.371
0.508HisHis: 0.508 ± 0.179
0.761HisIle: 0.761 ± 0.229
0.698HisLys: 0.698 ± 0.204
1.206HisLeu: 1.206 ± 0.256
0.317HisMet: 0.317 ± 0.128
0.444HisAsn: 0.444 ± 0.158
0.888HisPro: 0.888 ± 0.212
0.635HisGln: 0.635 ± 0.166
1.396HisArg: 1.396 ± 0.309
0.952HisSer: 0.952 ± 0.318
1.269HisThr: 1.269 ± 0.291
1.015HisVal: 1.015 ± 0.226
0.381HisTrp: 0.381 ± 0.148
1.015HisTyr: 1.015 ± 0.252
0.0HisXaa: 0.0 ± 0.0
Ile
5.52IleAla: 5.52 ± 0.592
0.571IleCys: 0.571 ± 0.186
3.807IleAsp: 3.807 ± 0.612
4.061IleGlu: 4.061 ± 0.638
0.888IlePhe: 0.888 ± 0.264
3.997IleGly: 3.997 ± 0.567
0.635IleHis: 0.635 ± 0.208
1.206IleIle: 1.206 ± 0.259
1.523IleLys: 1.523 ± 0.331
1.65IleLeu: 1.65 ± 0.355
0.444IleMet: 0.444 ± 0.212
1.459IleAsn: 1.459 ± 0.268
2.348IlePro: 2.348 ± 0.334
2.094IleGln: 2.094 ± 0.295
3.68IleArg: 3.68 ± 0.553
2.665IleSer: 2.665 ± 0.324
3.807IleThr: 3.807 ± 0.483
3.236IleVal: 3.236 ± 0.482
0.761IleTrp: 0.761 ± 0.184
1.142IleTyr: 1.142 ± 0.288
0.0IleXaa: 0.0 ± 0.0
Lys
4.569LysAla: 4.569 ± 0.584
0.317LysCys: 0.317 ± 0.123
1.459LysAsp: 1.459 ± 0.268
1.206LysGlu: 1.206 ± 0.263
1.396LysPhe: 1.396 ± 0.317
2.602LysGly: 2.602 ± 0.359
0.825LysHis: 0.825 ± 0.256
1.206LysIle: 1.206 ± 0.302
1.142LysLys: 1.142 ± 0.328
3.236LysLeu: 3.236 ± 0.46
0.508LysMet: 0.508 ± 0.183
0.825LysAsn: 0.825 ± 0.276
2.475LysPro: 2.475 ± 0.47
1.269LysGln: 1.269 ± 0.313
2.03LysArg: 2.03 ± 0.458
1.523LysSer: 1.523 ± 0.339
3.109LysThr: 3.109 ± 0.445
2.411LysVal: 2.411 ± 0.424
0.508LysTrp: 0.508 ± 0.194
1.015LysTyr: 1.015 ± 0.228
0.0LysXaa: 0.0 ± 0.0
Leu
10.533LeuAla: 10.533 ± 0.784
0.952LeuCys: 0.952 ± 0.246
5.33LeuAsp: 5.33 ± 0.459
3.934LeuGlu: 3.934 ± 0.463
1.967LeuPhe: 1.967 ± 0.331
6.789LeuGly: 6.789 ± 0.846
1.332LeuHis: 1.332 ± 0.333
4.949LeuIle: 4.949 ± 0.491
2.03LeuLys: 2.03 ± 0.323
6.282LeuLeu: 6.282 ± 0.594
1.396LeuMet: 1.396 ± 0.351
2.538LeuAsn: 2.538 ± 0.413
5.14LeuPro: 5.14 ± 0.445
2.03LeuGln: 2.03 ± 0.494
5.901LeuArg: 5.901 ± 0.639
3.997LeuSer: 3.997 ± 0.569
4.949LeuThr: 4.949 ± 0.453
5.14LeuVal: 5.14 ± 0.492
1.206LeuTrp: 1.206 ± 0.246
1.206LeuTyr: 1.206 ± 0.195
0.0LeuXaa: 0.0 ± 0.0
Met
2.855MetAla: 2.855 ± 0.489
0.127MetCys: 0.127 ± 0.085
1.142MetAsp: 1.142 ± 0.234
0.635MetGlu: 0.635 ± 0.195
0.381MetPhe: 0.381 ± 0.133
1.396MetGly: 1.396 ± 0.339
0.254MetHis: 0.254 ± 0.134
0.635MetIle: 0.635 ± 0.252
0.571MetLys: 0.571 ± 0.199
1.523MetLeu: 1.523 ± 0.276
0.19MetMet: 0.19 ± 0.116
0.825MetAsn: 0.825 ± 0.192
1.586MetPro: 1.586 ± 0.293
0.761MetGln: 0.761 ± 0.234
1.777MetArg: 1.777 ± 0.344
1.459MetSer: 1.459 ± 0.345
1.713MetThr: 1.713 ± 0.332
0.698MetVal: 0.698 ± 0.168
0.127MetTrp: 0.127 ± 0.078
0.254MetTyr: 0.254 ± 0.143
0.0MetXaa: 0.0 ± 0.0
Asn
3.109AsnAla: 3.109 ± 0.514
0.381AsnCys: 0.381 ± 0.167
1.967AsnAsp: 1.967 ± 0.382
1.84AsnGlu: 1.84 ± 0.362
0.761AsnPhe: 0.761 ± 0.172
3.046AsnGly: 3.046 ± 0.499
0.508AsnHis: 0.508 ± 0.181
1.142AsnIle: 1.142 ± 0.228
0.571AsnLys: 0.571 ± 0.223
2.411AsnLeu: 2.411 ± 0.349
0.254AsnMet: 0.254 ± 0.114
0.888AsnAsn: 0.888 ± 0.286
2.348AsnPro: 2.348 ± 0.317
1.142AsnGln: 1.142 ± 0.259
1.396AsnArg: 1.396 ± 0.387
2.03AsnSer: 2.03 ± 0.367
1.206AsnThr: 1.206 ± 0.284
2.094AsnVal: 2.094 ± 0.324
0.571AsnTrp: 0.571 ± 0.247
0.571AsnTyr: 0.571 ± 0.17
0.0AsnXaa: 0.0 ± 0.0
Pro
7.234ProAla: 7.234 ± 0.594
0.508ProCys: 0.508 ± 0.174
4.442ProAsp: 4.442 ± 0.481
3.617ProGlu: 3.617 ± 0.521
1.967ProPhe: 1.967 ± 0.366
6.98ProGly: 6.98 ± 1.17
1.015ProHis: 1.015 ± 0.263
1.777ProIle: 1.777 ± 0.335
2.284ProLys: 2.284 ± 0.427
3.363ProLeu: 3.363 ± 0.441
0.761ProMet: 0.761 ± 0.178
1.459ProAsn: 1.459 ± 0.368
4.188ProPro: 4.188 ± 0.544
2.03ProGln: 2.03 ± 0.298
4.569ProArg: 4.569 ± 0.602
3.49ProSer: 3.49 ± 0.54
3.109ProThr: 3.109 ± 0.445
4.251ProVal: 4.251 ± 0.41
1.523ProTrp: 1.523 ± 0.294
1.332ProTyr: 1.332 ± 0.22
0.0ProXaa: 0.0 ± 0.0
Gln
5.14GlnAla: 5.14 ± 0.581
0.635GlnCys: 0.635 ± 0.251
1.65GlnAsp: 1.65 ± 0.338
1.84GlnGlu: 1.84 ± 0.351
1.079GlnPhe: 1.079 ± 0.271
2.728GlnGly: 2.728 ± 0.584
1.079GlnHis: 1.079 ± 0.279
1.967GlnIle: 1.967 ± 0.372
1.967GlnLys: 1.967 ± 0.268
3.236GlnLeu: 3.236 ± 0.471
1.142GlnMet: 1.142 ± 0.263
0.761GlnAsn: 0.761 ± 0.176
2.03GlnPro: 2.03 ± 0.321
2.665GlnGln: 2.665 ± 0.52
2.538GlnArg: 2.538 ± 0.394
1.459GlnSer: 1.459 ± 0.355
3.109GlnThr: 3.109 ± 0.467
2.538GlnVal: 2.538 ± 0.354
0.761GlnTrp: 0.761 ± 0.183
0.444GlnTyr: 0.444 ± 0.152
0.0GlnXaa: 0.0 ± 0.0
Arg
7.868ArgAla: 7.868 ± 0.8
0.952ArgCys: 0.952 ± 0.229
5.266ArgAsp: 5.266 ± 0.612
4.188ArgGlu: 4.188 ± 0.606
2.284ArgPhe: 2.284 ± 0.353
5.203ArgGly: 5.203 ± 0.476
1.206ArgHis: 1.206 ± 0.203
3.807ArgIle: 3.807 ± 0.45
3.109ArgLys: 3.109 ± 0.44
4.822ArgLeu: 4.822 ± 0.694
1.904ArgMet: 1.904 ± 0.369
2.157ArgAsn: 2.157 ± 0.447
2.919ArgPro: 2.919 ± 0.44
3.109ArgGln: 3.109 ± 0.417
6.662ArgArg: 6.662 ± 0.904
2.665ArgSer: 2.665 ± 0.528
4.124ArgThr: 4.124 ± 0.512
4.188ArgVal: 4.188 ± 0.47
1.142ArgTrp: 1.142 ± 0.24
1.777ArgTyr: 1.777 ± 0.345
0.0ArgXaa: 0.0 ± 0.0
Ser
6.472SerAla: 6.472 ± 0.655
0.063SerCys: 0.063 ± 0.067
3.363SerAsp: 3.363 ± 0.409
2.919SerGlu: 2.919 ± 0.463
1.777SerPhe: 1.777 ± 0.342
6.409SerGly: 6.409 ± 1.066
0.698SerHis: 0.698 ± 0.197
2.411SerIle: 2.411 ± 0.356
1.586SerLys: 1.586 ± 0.472
3.236SerLeu: 3.236 ± 0.387
1.586SerMet: 1.586 ± 0.3
1.84SerAsn: 1.84 ± 0.385
3.236SerPro: 3.236 ± 0.561
1.777SerGln: 1.777 ± 0.338
3.363SerArg: 3.363 ± 0.437
2.221SerSer: 2.221 ± 0.372
2.728SerThr: 2.728 ± 0.525
3.934SerVal: 3.934 ± 0.432
1.079SerTrp: 1.079 ± 0.311
1.269SerTyr: 1.269 ± 0.242
0.0SerXaa: 0.0 ± 0.0
Thr
6.916ThrAla: 6.916 ± 0.744
0.444ThrCys: 0.444 ± 0.145
4.442ThrAsp: 4.442 ± 0.463
4.378ThrGlu: 4.378 ± 0.535
1.777ThrPhe: 1.777 ± 0.401
5.393ThrGly: 5.393 ± 0.693
1.269ThrHis: 1.269 ± 0.361
3.49ThrIle: 3.49 ± 0.528
2.348ThrLys: 2.348 ± 0.33
5.584ThrLeu: 5.584 ± 0.542
0.635ThrMet: 0.635 ± 0.216
1.523ThrAsn: 1.523 ± 0.283
4.632ThrPro: 4.632 ± 0.698
1.65ThrGln: 1.65 ± 0.339
3.426ThrArg: 3.426 ± 0.512
3.109ThrSer: 3.109 ± 0.425
3.871ThrThr: 3.871 ± 0.527
5.14ThrVal: 5.14 ± 0.66
1.332ThrTrp: 1.332 ± 0.262
1.523ThrTyr: 1.523 ± 0.297
0.0ThrXaa: 0.0 ± 0.0
Val
8.312ValAla: 8.312 ± 0.909
0.635ValCys: 0.635 ± 0.203
4.949ValAsp: 4.949 ± 0.623
4.759ValGlu: 4.759 ± 0.505
2.03ValPhe: 2.03 ± 0.386
5.266ValGly: 5.266 ± 0.628
1.84ValHis: 1.84 ± 0.379
2.538ValIle: 2.538 ± 0.453
2.03ValLys: 2.03 ± 0.361
4.949ValLeu: 4.949 ± 0.579
1.079ValMet: 1.079 ± 0.254
2.157ValAsn: 2.157 ± 0.285
3.807ValPro: 3.807 ± 0.524
2.855ValGln: 2.855 ± 0.441
4.632ValArg: 4.632 ± 0.742
3.744ValSer: 3.744 ± 0.498
5.14ValThr: 5.14 ± 0.814
5.964ValVal: 5.964 ± 0.749
1.586ValTrp: 1.586 ± 0.316
1.84ValTyr: 1.84 ± 0.346
0.0ValXaa: 0.0 ± 0.0
Trp
1.777TrpAla: 1.777 ± 0.301
0.571TrpCys: 0.571 ± 0.186
0.698TrpAsp: 0.698 ± 0.271
1.142TrpGlu: 1.142 ± 0.263
1.015TrpPhe: 1.015 ± 0.266
1.65TrpGly: 1.65 ± 0.273
0.444TrpHis: 0.444 ± 0.146
0.698TrpIle: 0.698 ± 0.192
0.508TrpLys: 0.508 ± 0.173
1.84TrpLeu: 1.84 ± 0.334
0.571TrpMet: 0.571 ± 0.185
0.571TrpAsn: 0.571 ± 0.217
0.888TrpPro: 0.888 ± 0.28
0.952TrpGln: 0.952 ± 0.272
1.015TrpArg: 1.015 ± 0.25
1.015TrpSer: 1.015 ± 0.226
1.269TrpThr: 1.269 ± 0.32
1.142TrpVal: 1.142 ± 0.296
0.761TrpTrp: 0.761 ± 0.199
0.698TrpTyr: 0.698 ± 0.225
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.855TyrAla: 2.855 ± 0.405
0.571TyrCys: 0.571 ± 0.167
1.967TyrAsp: 1.967 ± 0.363
1.332TyrGlu: 1.332 ± 0.276
0.698TyrPhe: 0.698 ± 0.186
2.221TyrGly: 2.221 ± 0.415
0.508TyrHis: 0.508 ± 0.176
0.508TyrIle: 0.508 ± 0.162
0.698TyrLys: 0.698 ± 0.195
1.713TyrLeu: 1.713 ± 0.303
0.254TyrMet: 0.254 ± 0.111
0.508TyrAsn: 0.508 ± 0.188
1.523TyrPro: 1.523 ± 0.256
0.698TyrGln: 0.698 ± 0.198
1.523TyrArg: 1.523 ± 0.338
1.523TyrSer: 1.523 ± 0.291
1.65TyrThr: 1.65 ± 0.315
1.65TyrVal: 1.65 ± 0.314
0.825TyrTrp: 0.825 ± 0.21
0.698TyrTyr: 0.698 ± 0.222
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (15761 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski